##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549925_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1493048 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.55081283388076 34.0 31.0 34.0 31.0 34.0 2 32.72411402714447 34.0 31.0 34.0 31.0 34.0 3 32.72997519168841 34.0 31.0 34.0 31.0 34.0 4 36.152283114809435 37.0 35.0 37.0 35.0 37.0 5 36.165408613788706 37.0 35.0 37.0 35.0 37.0 6 36.159787227202344 37.0 36.0 37.0 35.0 37.0 7 36.27967151759354 37.0 37.0 37.0 35.0 37.0 8 36.269492340500776 37.0 37.0 37.0 35.0 37.0 9 37.66511525416463 39.0 38.0 39.0 35.0 39.0 10 37.655890500506345 39.0 37.0 39.0 35.0 39.0 11 37.620575493889014 39.0 37.0 39.0 35.0 39.0 12 37.545659617105414 39.0 37.0 39.0 35.0 39.0 13 37.49684537938499 39.0 37.0 39.0 35.0 39.0 14 38.74945949493921 40.0 38.0 41.0 35.0 41.0 15 38.78818296531659 40.0 38.0 41.0 35.0 41.0 16 38.81408836152622 40.0 38.0 41.0 35.0 41.0 17 38.770880105663046 40.0 38.0 41.0 35.0 41.0 18 38.71826357893383 40.0 38.0 41.0 35.0 41.0 19 38.684981996560055 40.0 38.0 41.0 35.0 41.0 20 38.64570328616361 40.0 38.0 41.0 35.0 41.0 21 38.61500367034416 40.0 38.0 41.0 35.0 41.0 22 38.59200374000032 40.0 38.0 41.0 35.0 41.0 23 38.56235231553172 40.0 38.0 41.0 35.0 41.0 24 38.53879781493964 40.0 38.0 41.0 35.0 41.0 25 38.44256514191105 40.0 38.0 41.0 35.0 41.0 26 38.33381110319293 40.0 37.0 41.0 34.0 41.0 27 38.255330036274785 40.0 37.0 41.0 34.0 41.0 28 38.19241578301568 40.0 37.0 41.0 34.0 41.0 29 38.08342598496498 40.0 37.0 41.0 34.0 41.0 30 37.97776025954959 40.0 37.0 41.0 34.0 41.0 31 37.85908490550873 40.0 36.0 41.0 34.0 41.0 32 37.730551864374085 40.0 36.0 41.0 33.0 41.0 33 37.667855286635124 40.0 36.0 41.0 33.0 41.0 34 37.53656613853004 40.0 36.0 41.0 33.0 41.0 35 37.41414140737605 40.0 36.0 41.0 33.0 41.0 36 37.306284191800934 40.0 35.0 41.0 33.0 41.0 37 37.23572852312853 40.0 35.0 41.0 32.0 41.0 38 37.1129896694547 39.0 35.0 41.0 32.0 41.0 39 37.04020902208101 39.0 35.0 41.0 32.0 41.0 40 36.85837427865682 39.0 35.0 41.0 31.0 41.0 41 36.70020052938686 39.0 35.0 41.0 31.0 41.0 42 36.575443656198594 39.0 35.0 41.0 31.0 41.0 43 36.548684971949996 39.0 35.0 41.0 31.0 41.0 44 36.45741664032235 39.0 35.0 41.0 31.0 41.0 45 36.32365603785009 39.0 35.0 41.0 30.0 41.0 46 36.14958862675547 38.0 35.0 40.0 30.0 41.0 47 36.0560645069683 38.0 35.0 40.0 30.0 41.0 48 35.92679337837765 38.0 35.0 40.0 30.0 41.0 49 35.84923726497742 38.0 35.0 40.0 29.0 41.0 50 35.73443117702847 38.0 35.0 40.0 29.0 41.0 51 33.98959176128296 36.0 33.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 27.0 9 58.0 10 65.0 11 47.0 12 37.0 13 31.0 14 47.0 15 65.0 16 96.0 17 203.0 18 321.0 19 583.0 20 1006.0 21 1748.0 22 2495.0 23 3881.0 24 5699.0 25 7650.0 26 9363.0 27 11378.0 28 13180.0 29 15819.0 30 18920.0 31 23516.0 32 30685.0 33 42588.0 34 81488.0 35 112174.0 36 103046.0 37 164627.0 38 318310.0 39 523662.0 40 230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.450144938407874 20.154341990344584 24.115299709051552 11.280213362195992 2 32.938659708194244 24.38186849987408 24.490639282862976 18.188832509068696 3 32.330038953871544 24.016977351029574 25.869965332661778 17.783018362437108 4 29.047291178850244 25.893139403421728 24.87883845663368 20.18073096109435 5 24.0757162529269 31.268050323901175 23.470712261092743 21.185521162079183 6 25.948797359495472 34.91943996442177 24.057565463401044 15.074197212681709 7 81.17528706377826 5.823322491976145 7.865386779259609 5.136003664985989 8 80.7765055108744 5.325012993554125 8.098534005604643 5.799947489966833 9 73.92823271589393 7.495338395014761 10.989733752699177 7.586695136392133 10 38.598826025687046 25.84243775149895 18.427471856229673 17.13126436658433 11 29.288475655169826 25.66166660415472 23.60875202940562 21.44110571126983 12 27.31318751975824 21.19710819745916 30.51562977211717 20.97407451066543 13 25.421352829915715 23.51270689220976 30.31878412482385 20.74715615305067 14 20.354335560544605 27.869030332581403 28.655877105089722 23.12075700178427 15 18.950294967074065 25.458592088131127 34.028644758909294 21.562468185885518 16 21.105617501915543 23.976255284491856 31.456992675386193 23.46113453820641 17 20.888879660935213 23.817720528743884 28.217378141894965 27.07602166842593 18 21.829438839206777 25.052643987333294 29.584514362565706 23.533402810894223 19 23.72951171027321 26.545630147188838 26.513146261875036 23.211711880662914 20 25.40105877372998 25.35524644887505 26.443222187096467 22.800472590298504 21 24.356819070786738 26.005928811397894 27.704199731020037 21.933052386795335 22 21.615647989883783 24.369008899914803 26.09239622570741 27.922946884494003 23 21.425232142570096 26.63725479689869 26.403370822639328 25.53414223789188 24 23.112987660142206 24.472421516254 28.132384223414114 24.282206600189678 25 22.724051738457167 25.9189255804234 25.99561434059722 25.361408340522207 26 20.934691985790142 27.49382471293622 26.10893956523836 25.462543736035276 27 22.055821380156566 26.081545938241774 27.065238358043413 24.79739432355825 28 20.510124255884605 25.9564327469713 28.00171193424458 25.53173106289952 29 21.162548022568597 26.041158757119664 26.982722591638048 25.813570628673695 30 23.330730157369352 24.39908161023624 25.839624714007854 26.430563518386545 31 23.835469455771012 26.738390192411764 23.611765998146076 25.81437435367115 32 22.292585368990146 26.898532398154646 25.560062369059803 25.2488198637954 33 22.46739555593658 24.30765789177575 25.482368952639167 27.742577599648506 34 21.759179879012596 24.283747073101466 27.05003456017489 26.907038487711045 35 21.690997208395178 23.13957756214134 27.69468898521682 27.474736244246667 36 22.131907346582295 25.315662992750397 26.520915603517096 26.031514057150208 37 21.77063296022633 24.75553364660748 28.40357443297201 25.07025896019418 38 21.03502365630576 24.926861025231606 28.107066885994286 25.931048432468344 39 22.554599718160436 22.398543114487946 28.64482588637472 26.4020312809769 40 23.566824375371723 22.56960258477959 30.200435618948617 23.663137420900064 41 20.489830199698872 24.214827654569714 28.682199098756367 26.613143046975047 42 21.13676184556692 24.52707481608093 29.016883583113202 25.31927975523895 43 22.32781531471192 22.757205394602185 28.74448778605912 26.170491504626774 44 21.88148003279198 23.05056501867321 27.58035910432886 27.48759584420595 45 22.282136944023236 22.410264104034162 27.1585374348313 28.149061517111306 46 22.869057123414652 23.607881327325043 27.600452229265233 25.922609319995072 47 21.67445386886423 23.74384480606116 29.793147976488367 24.788553348586248 48 20.531623899566526 22.57067421810953 30.053822783996225 26.843879098327715 49 21.369708140662592 21.91436578060451 30.479194238899222 26.23673183983368 50 20.413744233273142 21.895679174413683 30.100773719264218 27.589802873048956 51 20.99182343769256 20.93194592538217 27.942571169848524 30.133659467076747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 785.0 1 1569.5 2 2354.0 3 3360.0 4 4366.0 5 3368.5 6 2371.0 7 2264.5 8 2158.0 9 2191.0 10 2224.0 11 2458.5 12 2693.0 13 2771.0 14 2849.0 15 2846.0 16 2843.0 17 2892.5 18 2942.0 19 3115.0 20 3288.0 21 3359.0 22 3430.0 23 4011.5 24 4593.0 25 5851.0 26 8345.0 27 9581.0 28 11367.0 29 13153.0 30 15909.5 31 18666.0 32 21258.5 33 23851.0 34 27364.5 35 30878.0 36 32897.0 37 34916.0 38 39042.0 39 43168.0 40 48052.5 41 52937.0 42 60681.0 43 68425.0 44 81145.5 45 93866.0 46 110811.5 47 127757.0 48 143075.5 49 158394.0 50 157371.0 51 156348.0 52 137080.0 53 117812.0 54 103703.0 55 89594.0 56 81718.0 57 73842.0 58 72975.5 59 72109.0 60 69052.5 61 65996.0 62 59936.0 63 53876.0 64 48804.0 65 43732.0 66 37217.5 67 30703.0 68 26283.0 69 21863.0 70 19023.5 71 16184.0 72 14500.5 73 12817.0 74 10242.5 75 6310.0 76 4952.0 77 3910.0 78 2868.0 79 2199.0 80 1530.0 81 1190.5 82 851.0 83 625.0 84 399.0 85 270.5 86 142.0 87 108.5 88 75.0 89 56.0 90 37.0 91 29.5 92 22.0 93 24.0 94 26.0 95 14.0 96 2.0 97 1.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1493048.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.422554550358733 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81571098990406 18.447094321028196 2 11.918787184461825 6.298496091111183 3 4.866210501273784 3.8573313727030523 4 2.464577428581692 2.6048172620113044 5 1.505608054538375 1.9891005476249852 6 0.9817846829146399 1.5564755604611233 7 0.6826906345563749 1.2626901372809387 8 0.529393546061003 1.1190343879523759 9 0.4159190305331386 0.9890678945514757 >10 5.726654101615279 38.83717382936928 >50 1.0017244185917624 16.351298125989043 >100 0.08276509654198852 4.036048838904782 >500 0.005619852167913216 1.0111551958707363 >1k 0.002299030432328134 1.2822184938226007 >5k 2.554478258142371E-4 0.35799794131889634 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC 5304 0.3552464488750529 TruSeq Adapter, Index 15 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC 4921 0.3295942260396183 TruSeq Adapter, Index 15 (95% over 21bp) CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT 3405 0.22805696802781958 TruSeq Adapter, Index 14 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.13576254748675193 No Hit TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC 1821 0.12196526836377665 TruSeq Adapter, Index 15 (95% over 21bp) CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG 1544 0.1034126163391934 TruSeq Adapter, Index 14 (95% over 21bp) GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT 1520 0.10180516634428363 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3395416624247848E-4 0.0 0.0 0.2587324720973472 0.0 2 1.3395416624247848E-4 0.0 0.0 1.2922558417411898 0.0 3 1.3395416624247848E-4 0.0 0.0 1.8472949295668994 0.0 4 1.3395416624247848E-4 0.0 0.0 2.4052810090499435 0.0 5 1.3395416624247848E-4 0.0 0.0 3.9553986208079044 0.0 6 1.3395416624247848E-4 0.0 0.0 4.9861759300437765 0.0 7 1.3395416624247848E-4 0.0 0.0 5.963170641533293 0.0 8 1.3395416624247848E-4 0.0 0.0 7.56874527811564 0.0 9 1.3395416624247848E-4 0.0 0.0 8.249835236375521 0.0 10 1.3395416624247848E-4 0.0 0.0 9.606121169580616 0.0 11 1.3395416624247848E-4 0.0 0.0 11.374851980646302 0.0 12 1.3395416624247848E-4 0.0 0.0 12.755517572107527 0.0 13 1.3395416624247848E-4 0.0 0.0 13.308279439107116 0.0 14 1.3395416624247848E-4 0.0 0.0 13.534126163391933 0.0 15 1.3395416624247848E-4 0.0 0.0 13.94737476624998 0.0 16 1.3395416624247848E-4 0.0 0.0 14.840514169671705 0.0 17 1.3395416624247848E-4 0.0 0.0 15.903038616307045 0.0 18 2.6790833248495696E-4 0.0 0.0 17.157318451918492 0.0 19 2.6790833248495696E-4 0.0 0.0 17.76245639791889 0.0 20 2.6790833248495696E-4 0.0 0.0 18.37342135015083 0.0 21 2.6790833248495696E-4 0.0 0.0 19.101127358263096 0.0 22 2.6790833248495696E-4 0.0 0.0 19.926084091067402 0.0 23 2.6790833248495696E-4 0.0 0.0 20.78921776125081 0.0 24 2.6790833248495696E-4 0.0 0.0 21.424629348822005 0.0 25 2.6790833248495696E-4 0.0 0.0 21.9950731657656 0.0 26 2.6790833248495696E-4 0.0 0.0 22.512672064126537 0.0 27 2.6790833248495696E-4 0.0 0.0 23.020559285434896 0.0 28 2.6790833248495696E-4 0.0 0.0 23.5591889878959 0.0 29 2.6790833248495696E-4 0.0 0.0 24.155887821423022 0.0 30 2.6790833248495696E-4 0.0 0.0 24.889621767016198 0.0 31 2.6790833248495696E-4 0.0 0.0 25.45765440896743 0.0 32 2.6790833248495696E-4 0.0 0.0 26.011086046798226 0.0 33 2.6790833248495696E-4 0.0 0.0 26.544960376357626 0.0 34 2.6790833248495696E-4 0.0 0.0 27.064903472627805 0.0 35 2.6790833248495696E-4 0.0 0.0 27.658856245746954 0.0 36 3.348854156061962E-4 0.0 0.0 28.1856310044955 0.0 37 3.348854156061962E-4 0.0 0.0 28.716424388231324 0.0 38 3.348854156061962E-4 0.0 0.0 29.221699503297952 0.0 39 3.348854156061962E-4 0.0 0.0 29.747804491215287 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAACG 35 1.2122473E-7 45.000004 1 ACTATCG 20 7.033966E-4 45.0 22 TCTAGCG 55 1.8189894E-12 45.0 1 CTACGTC 20 7.033966E-4 45.0 34 ATAAGCG 55 1.8189894E-12 45.0 1 TACTCGT 25 3.891368E-5 45.0 39 TATACGG 45 3.8562575E-10 45.0 2 CGACGGT 100 0.0 42.75 28 GCCGATA 90 0.0 42.5 9 CGTTGAT 45 1.9290383E-8 40.0 25 CGGTCTA 110 0.0 38.863636 31 ATAGCGG 195 0.0 38.076923 2 CGTTCGG 415 0.0 37.95181 45 TACGAAG 90 0.0 37.5 1 TTATCCG 30 1.1400855E-4 37.499996 1 ATCGCGA 30 1.1400855E-4 37.499996 38 ATGCGAA 325 0.0 37.384617 26 CGAATAT 200 0.0 37.125 14 CACAACG 195 0.0 36.923077 12 AATGCGA 330 0.0 36.818184 25 >>END_MODULE