FastQCFastQC Report
Sat 18 Jun 2016
SRR3549924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497133
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC28690.5771091438307254No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC24420.4912166361919245No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT16400.3298915984253711No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC9560.19230266347235048No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.14784775905039496No Hit
ACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC7000.14080738957180472No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG6760.13597970764362857No Hit
TTAGTAGGGAAGAATTCTTTTATTGCTGTTTCTTTATTTCCTTGTAAAGAT6220.1251174233052322No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG207.0294185E-445.0000041
TAAACGG207.0294185E-445.0000042
TAACGAC207.0294185E-445.00000414
AATGCGC207.0294185E-445.00000435
AAACCCG207.0294185E-445.00000433
CTTGACG207.0294185E-445.0000041
TTAACGG406.8012014E-945.0000042
TTAACGA207.0294185E-445.00000413
TTACGAG207.0294185E-445.0000041
CGGTCAT207.0294185E-445.00000438
GCTAATT207.0294185E-445.00000432
ACCTTGC207.0294185E-445.00000411
ACGGTCA207.0294185E-445.00000437
TTTAGCG253.887601E-545.01
CAAACGT253.887601E-545.027
ACACGCG1000.045.036
GGTAACG253.887601E-545.01
CAGTTCG253.887601E-545.012
TTATGCG351.2100645E-745.01
CGCAGTA351.2100645E-745.037