##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549923_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451050
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51049329342645	33.0	31.0	34.0	31.0	34.0
2	32.6923068395965	34.0	31.0	34.0	31.0	34.0
3	32.70032590621882	34.0	31.0	34.0	31.0	34.0
4	36.13425119166389	37.0	35.0	37.0	35.0	37.0
5	36.1377652144995	37.0	35.0	37.0	35.0	37.0
6	36.1285356390644	37.0	35.0	37.0	35.0	37.0
7	36.19965857443742	37.0	36.0	37.0	35.0	37.0
8	36.18164726748697	37.0	36.0	37.0	35.0	37.0
9	37.605205631304734	39.0	38.0	39.0	35.0	39.0
10	37.64328123267931	39.0	37.0	39.0	35.0	39.0
11	37.58906773085024	39.0	37.0	39.0	35.0	39.0
12	37.347872741381224	39.0	37.0	39.0	35.0	39.0
13	37.19606473783394	39.0	37.0	39.0	34.0	39.0
14	38.33987806229908	40.0	37.0	41.0	34.0	41.0
15	38.433153752355615	40.0	37.0	41.0	35.0	41.0
16	38.49281676089125	40.0	37.0	41.0	35.0	41.0
17	38.455996009311605	40.0	37.0	41.0	35.0	41.0
18	38.385079259505595	40.0	37.0	41.0	35.0	41.0
19	38.3332357831726	40.0	37.0	41.0	34.0	41.0
20	38.28883494069394	40.0	36.0	41.0	34.0	41.0
21	38.23621993127148	40.0	36.0	41.0	34.0	41.0
22	38.16154971732624	40.0	36.0	41.0	34.0	41.0
23	38.134441857887154	40.0	36.0	41.0	34.0	41.0
24	38.12900787052433	40.0	36.0	41.0	34.0	41.0
25	38.045533754572666	40.0	35.0	41.0	34.0	41.0
26	37.93406717658796	40.0	35.0	41.0	34.0	41.0
27	37.88719654140339	40.0	35.0	41.0	34.0	41.0
28	37.81459483427558	40.0	35.0	41.0	34.0	41.0
29	37.71981598492407	40.0	35.0	41.0	34.0	41.0
30	37.63306728744042	40.0	36.0	41.0	33.0	41.0
31	37.43310719432436	40.0	35.0	41.0	33.0	41.0
32	37.26681520895688	39.0	35.0	41.0	33.0	41.0
33	37.158521228245206	39.0	35.0	41.0	33.0	41.0
34	36.99780290433433	39.0	35.0	41.0	32.0	41.0
35	36.922680412371136	39.0	35.0	41.0	32.0	41.0
36	36.814592617226474	39.0	35.0	41.0	32.0	41.0
37	36.6171555259949	39.0	35.0	41.0	31.0	41.0
38	36.49029819310498	39.0	35.0	41.0	31.0	41.0
39	36.380518789491184	39.0	35.0	41.0	31.0	41.0
40	36.191304733399846	39.0	35.0	41.0	30.0	41.0
41	36.12717215386321	38.0	35.0	40.0	30.0	41.0
42	36.013422015297635	38.0	35.0	40.0	30.0	41.0
43	35.90850238332779	38.0	35.0	40.0	30.0	41.0
44	35.79883604921849	38.0	35.0	40.0	30.0	41.0
45	35.660086464915196	38.0	35.0	40.0	30.0	41.0
46	35.53352399955659	38.0	35.0	40.0	29.0	41.0
47	35.46076488194213	38.0	35.0	40.0	29.0	41.0
48	35.34685289879171	37.0	35.0	40.0	28.0	41.0
49	35.26662897683184	37.0	35.0	40.0	28.0	41.0
50	35.078722979714	37.0	34.0	40.0	27.0	41.0
51	33.31573661456601	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	22.0
10	20.0
11	9.0
12	7.0
13	15.0
14	20.0
15	29.0
16	44.0
17	61.0
18	150.0
19	241.0
20	460.0
21	739.0
22	1136.0
23	1823.0
24	2736.0
25	3225.0
26	3646.0
27	3860.0
28	4536.0
29	5130.0
30	6024.0
31	7586.0
32	9978.0
33	14201.0
34	29506.0
35	47197.0
36	30446.0
37	48822.0
38	93482.0
39	135822.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22758009089902	21.657687617780734	24.052100654029488	11.062631637290767
2	31.388759561024276	24.895466134574882	24.792373351069728	18.923400953331114
3	30.52743598270702	25.942578428112185	26.631193880944465	16.898791708236338
4	26.80146325241104	27.1315818645383	24.59505598048997	21.47189890256069
5	22.5826405054872	32.76399512249196	24.09511140671766	20.558252965303183
6	24.17514687950338	35.027158851568565	24.979270590843587	15.81842367808447
7	78.76399512249196	7.592506374016185	8.43476333000776	5.208735173484093
8	78.11905553708016	6.0840261611794695	9.117392750249419	6.679525551490966
9	71.3818867087906	7.482319033366588	12.671100764881944	8.464693492960869
10	40.999889147544614	23.747478106640063	19.679414699035583	15.573218046779736
11	32.323910874625874	25.04356501496508	23.09234009533311	19.540184015075933
12	28.94113734619222	20.444296641170602	30.91142888814987	19.70313712448731
13	25.43864316594612	25.796918301740384	30.91941026493737	17.845028267376122
14	18.186010420130806	30.179580977718658	29.209178583305622	22.425230018844918
15	16.77485866311939	24.61678306174482	38.0789269482319	20.52943132690389
16	19.90089790488859	24.674869748364927	32.003768983482985	23.420463363263497
17	19.892916528101097	23.827735284336548	26.082474226804127	30.196873960758232
18	21.807116727635517	25.03868750692828	29.90843587185456	23.245759893581646
19	22.72142777962532	27.49761667220929	25.530428999002332	24.250526549163066
20	24.896352954217935	25.78472453164838	25.70335882939807	23.615563684735616
21	23.2668218601042	27.431992018623212	27.22735838598825	22.073827735284336
22	20.18268484646935	24.847577873849904	27.133577208735172	27.83616007094557
23	19.455714444074935	27.0293759006762	26.558474670213943	26.956434985034917
24	22.409710675091453	23.831504267819533	29.15752133909766	24.601263717991355
25	20.2760226139009	26.423456379558807	26.88837157743044	26.412149429109853
26	18.64760004434098	28.64893027380557	27.97938144329897	24.724088238554483
27	20.65513801130695	26.135018290655136	30.71100764881942	22.49883604921849
28	19.74858663119388	25.41048664227913	31.369471233787827	23.471455492739164
29	21.53885378561135	26.81786941580756	27.64327679858109	24.0
30	24.78261833499612	26.438754018401507	27.209621993127147	21.569005653475223
31	24.16982596164505	30.91076377341758	23.34375346413923	21.575656800798136
32	23.077042456490414	31.52000886819643	21.874071610686176	23.528877064626982
33	24.656024830950006	27.12404389757233	24.86420574215719	23.355725529320477
34	21.117171045338655	26.90211728189779	27.783394302183794	24.197317370579757
35	20.70324797694269	26.821194989469017	26.23035140228356	26.245205631304735
36	26.1352399955659	25.011417802904333	27.64527214277796	21.2080700587518
37	21.86897239773861	27.57743044008425	29.23689169715109	21.31670546502605
38	22.989247311827956	29.561024276687732	23.939031149539964	23.51069726194435
39	26.41104090455604	25.73661456601264	25.021172818977938	22.831171710453386
40	25.182130584192443	24.570225030484423	26.881277020286	23.366367365037135
41	21.867420463363263	24.40416805232236	25.133133798913647	28.59527768540073
42	24.12526327458153	23.620884602593947	26.047001441081918	26.2068506817426
43	23.473672541846803	22.434541625096998	29.747921516461588	24.343864316594612
44	21.489857000332556	23.8720762664893	29.781842367808448	24.85622436536969
45	21.605365258840482	26.06717658796142	26.906329675202308	25.421128477995786
46	24.824963972952002	25.65369692938699	27.403170380223923	22.118168717437094
47	20.073827735284336	25.859217381665005	29.745261057532424	24.321693825518235
48	21.710675091453275	23.041347965857444	28.812770202859994	26.435206739829287
49	24.065402948675313	19.932823412038577	30.9859217381665	25.01585190111961
50	21.05176809666334	20.782174925174594	30.846247644385322	27.319809333776746
51	20.343864316594612	22.012415475002772	26.4877508036803	31.155969404722317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	670.0
1	851.5
2	1033.0
3	1319.0
4	1605.0
5	1336.0
6	1067.0
7	1087.5
8	1108.0
9	1224.5
10	1341.0
11	1404.0
12	1467.0
13	1475.5
14	1484.0
15	1469.0
16	1454.0
17	1446.5
18	1439.0
19	1356.0
20	1273.0
21	1345.0
22	1417.0
23	1397.0
24	1377.0
25	1382.0
26	1777.0
27	2167.0
28	2243.0
29	2319.0
30	2692.0
31	3065.0
32	3628.5
33	4192.0
34	5577.5
35	6963.0
36	7584.0
37	8205.0
38	9230.0
39	10255.0
40	14001.5
41	17748.0
42	24219.5
43	30691.0
44	38192.5
45	45694.0
46	51377.0
47	57060.0
48	56272.0
49	55484.0
50	46107.0
51	36730.0
52	31583.0
53	26436.0
54	23743.0
55	21050.0
56	19683.0
57	18316.0
58	17728.0
59	17140.0
60	17039.5
61	16939.0
62	17041.5
63	17144.0
64	14481.0
65	11818.0
66	9790.5
67	7763.0
68	6222.5
69	4682.0
70	4270.0
71	3858.0
72	3359.0
73	2860.0
74	2290.5
75	1307.0
76	893.0
77	753.5
78	614.0
79	614.0
80	614.0
81	447.0
82	280.0
83	197.0
84	114.0
85	82.5
86	51.0
87	37.5
88	24.0
89	18.0
90	12.0
91	12.5
92	13.0
93	10.5
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	451050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.488436334054235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79955388583865	22.19544563795942
2	11.380245610763573	6.939317875393308
3	4.453266504592984	4.073193969115783
4	2.2429685369578327	2.7353841375330266
5	1.3015336554901198	1.9840862996019701
6	0.8903919478237257	1.6287994928146925
7	0.6547758547294775	1.3974164371996933
8	0.4916132805576299	1.1990816164205473
9	0.3651886114906856	1.002062675821989
>10	4.556491050536117	34.34859821108213
>50	0.7821749844174265	14.766394750597895
>100	0.07230188931589655	3.9625248576027277
>500	0.004381932685811913	0.9216054386164133
>1k	0.005112254800113898	2.846088600240395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2770	0.6141226028156523	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2587	0.5735506041458819	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	2014	0.4465136902782396	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	1912	0.4238997893803348	No Hit
GGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	1276	0.2828954661345749	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1217	0.26981487639951224	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1006	0.22303514022835605	No Hit
TGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	866	0.1919964527214278	No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	765	0.16960425673428667	No Hit
GTGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGC	706	0.15652366699922404	No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	681	0.1509810442301297	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.13523999556590177	No Hit
AAAACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC	511	0.11329120940028821	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	499	0.11063075047112293	No Hit
GCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTA	488	0.10819199645272144	No Hit
AGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	469	0.10397960314820973	No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG	462	0.10242766877286331	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	456	0.10109743930828068	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2170491076377343E-4	0.0	0.0	0.5043786719875846	0.0
2	2.2170491076377343E-4	0.0	0.0	2.293537301851236	0.0
3	2.2170491076377343E-4	0.0	0.0	3.1326903890921183	0.0
4	2.2170491076377343E-4	0.0	0.0	3.998004655803126	0.0
5	2.2170491076377343E-4	0.0	0.0	6.277796253187008	0.0
6	2.2170491076377343E-4	0.0	0.0	7.604478439197428	0.0
7	2.2170491076377343E-4	0.0	0.0	8.90655138011307	0.0
8	2.2170491076377343E-4	0.0	0.0	11.252189335993792	0.0
9	2.2170491076377343E-4	0.0	0.0	12.223478550049883	0.0
10	2.2170491076377343E-4	0.0	0.0	14.020175146879504	0.0
11	2.2170491076377343E-4	0.0	0.0	16.305731071943242	0.0
12	2.2170491076377343E-4	0.0	0.0	18.073606030373572	0.0
13	2.2170491076377343E-4	0.0	0.0	18.736060303735727	0.0
14	2.2170491076377343E-4	0.0	0.0	18.97816206628977	0.0
15	2.2170491076377343E-4	0.0	0.0	19.4534973949673	0.0
16	2.2170491076377343E-4	0.0	0.0	20.47777408269593	0.0
17	2.2170491076377343E-4	0.0	0.0	21.832834497284114	0.0
18	2.2170491076377343E-4	0.0	0.0	23.639064405276578	0.0
19	2.2170491076377343E-4	0.0	0.0	24.38842700365813	0.0
20	2.2170491076377343E-4	0.0	0.0	25.1340206185567	0.0
21	2.2170491076377343E-4	0.0	0.0	25.892694823190332	0.0
22	2.2170491076377343E-4	0.0	0.0	26.671765879614235	0.0
23	2.2170491076377343E-4	0.0	0.0	27.540184015075933	0.0
24	2.2170491076377343E-4	0.0	0.0	28.107526881720432	0.0
25	4.4340982152754686E-4	0.0	0.0	28.64737833943022	0.0
26	4.4340982152754686E-4	0.0	0.0	29.09278350515464	0.0
27	4.4340982152754686E-4	0.0	0.0	29.563019620884603	0.0
28	4.4340982152754686E-4	0.0	0.0	30.058086686620108	0.0
29	4.4340982152754686E-4	0.0	0.0	30.699257288548942	0.0
30	4.4340982152754686E-4	0.0	0.0	31.567232014189113	0.0
31	4.4340982152754686E-4	0.0	0.0	32.06274248974615	0.0
32	4.4340982152754686E-4	0.0	0.0	32.52544063851014	0.0
33	4.4340982152754686E-4	0.0	0.0	33.009644163618226	0.0
34	4.4340982152754686E-4	0.0	0.0	33.50471122935373	0.0
35	4.4340982152754686E-4	0.0	0.0	34.055426227690944	0.0
36	4.4340982152754686E-4	0.0	0.0	34.51302516350737	0.0
37	4.4340982152754686E-4	0.0	0.0	34.973949672985256	0.0
38	4.4340982152754686E-4	0.0	0.0	35.42112847799579	0.0
39	4.4340982152754686E-4	0.0	0.0	35.94302183793371	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTTAG	45	3.8380676E-10	45.000004	1
ACTATAT	20	7.028725E-4	45.0	22
CTCACGT	25	3.887024E-5	45.0	45
GCGACGT	25	3.887024E-5	45.0	17
GGTAAAC	25	3.887024E-5	45.0	8
GACCGTT	20	7.028725E-4	45.0	15
CGCATAC	20	7.028725E-4	45.0	11
ACCGAGT	20	7.028725E-4	45.0	21
CCTACCG	20	7.028725E-4	45.0	36
ATTCGTC	20	7.028725E-4	45.0	30
ATTCGCG	20	7.028725E-4	45.0	1
CAACACG	20	7.028725E-4	45.0	1
CCTCGTG	155	0.0	45.0	15
ATCACGG	25	3.887024E-5	45.0	2
TATTGAG	20	7.028725E-4	45.0	1
ATTCCCC	20	7.028725E-4	45.0	42
ATTCCAC	20	7.028725E-4	45.0	16
TCAATCG	25	3.887024E-5	45.0	43
TGACCTC	20	7.028725E-4	45.0	14
TACGCGA	20	7.028725E-4	45.0	1
>>END_MODULE