FastQCFastQC Report
Sat 18 Jun 2016
SRR3549920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304106
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26990.2069617040332611No Hit
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC25000.19170220825607737RNA PCR Primer, Index 30 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC20040.1536684901380716Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17520.13434490754585898No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT14580.11180072785494431RNA PCR Primer, Index 30 (96% over 25bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13310.10206225567553556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA302.165727E-645.00000428
CCGTCGA207.033636E-445.035
ATCGTAG253.8910955E-545.01
GTCGTAC406.8157533E-945.045
TGCGTTA207.033636E-445.034
GTACGAG650.041.5384641
CGAATAT3100.039.19354614
GCTACGA3300.038.18181610
CTATACG659.094947E-1238.0769271
TATTGCG1250.037.81
GCGATAT1250.037.89
TCGACCC301.1400061E-437.50000438
AGTAACG601.5643309E-1037.5000041
TATACGG1150.037.1739122
TAGGGTA3700.037.0945935
CTACGAA3350.036.940311
TCGACGT552.750312E-936.8181826
ATCTACG504.879803E-836.01
TACCACG250.002107212336.01
ATTACGA250.002107212336.012
Produced by FastQC (version 0.11.5)