##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549920_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1304106 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.666552412150544 34.0 31.0 34.0 31.0 34.0 2 32.85413302292912 34.0 31.0 34.0 31.0 34.0 3 32.840281388169366 34.0 31.0 34.0 31.0 34.0 4 36.246303598020404 37.0 37.0 37.0 35.0 37.0 5 36.25113986133029 37.0 37.0 37.0 35.0 37.0 6 36.24240590872214 37.0 37.0 37.0 35.0 37.0 7 36.31880000552103 37.0 37.0 37.0 35.0 37.0 8 36.31672272039236 37.0 37.0 37.0 35.0 37.0 9 37.700301969318446 39.0 38.0 39.0 35.0 39.0 10 37.758075647225 39.0 37.0 39.0 35.0 39.0 11 37.71520566579711 39.0 37.0 39.0 35.0 39.0 12 37.58605665490382 39.0 37.0 39.0 35.0 39.0 13 37.547648734075295 39.0 37.0 39.0 35.0 39.0 14 38.8450049305808 40.0 38.0 41.0 35.0 41.0 15 38.88628378368016 40.0 38.0 41.0 35.0 41.0 16 38.88466965108665 40.0 38.0 41.0 35.0 41.0 17 38.83781993181536 40.0 38.0 41.0 35.0 41.0 18 38.771373646007305 40.0 38.0 41.0 35.0 41.0 19 38.73998586004512 40.0 38.0 41.0 35.0 41.0 20 38.69109412885149 40.0 38.0 41.0 35.0 41.0 21 38.66884517056129 40.0 38.0 41.0 35.0 41.0 22 38.6225038455463 40.0 38.0 41.0 35.0 41.0 23 38.58100108426769 40.0 38.0 41.0 35.0 41.0 24 38.55159166509471 40.0 38.0 41.0 35.0 41.0 25 38.45802871852442 40.0 37.0 41.0 35.0 41.0 26 38.36986180571211 40.0 37.0 41.0 34.0 41.0 27 38.309717921702685 40.0 37.0 41.0 34.0 41.0 28 38.21075970818323 40.0 37.0 41.0 34.0 41.0 29 38.12826948116181 40.0 37.0 41.0 34.0 41.0 30 38.029204681214566 40.0 37.0 41.0 34.0 41.0 31 37.91040068828761 40.0 36.0 41.0 34.0 41.0 32 37.81810911076247 40.0 36.0 41.0 33.0 41.0 33 37.71623932410402 40.0 36.0 41.0 33.0 41.0 34 37.55969836807744 40.0 36.0 41.0 33.0 41.0 35 37.47349371906885 40.0 36.0 41.0 33.0 41.0 36 37.37785502098756 40.0 35.0 41.0 33.0 41.0 37 37.27829562934301 40.0 35.0 41.0 33.0 41.0 38 37.15006218819636 40.0 35.0 41.0 32.0 41.0 39 37.04539968376804 39.0 35.0 41.0 32.0 41.0 40 36.886642650214014 39.0 35.0 41.0 32.0 41.0 41 36.78306824751976 39.0 35.0 41.0 31.0 41.0 42 36.67833136263463 39.0 35.0 41.0 31.0 41.0 43 36.622420263383496 39.0 35.0 41.0 31.0 41.0 44 36.526956397716134 39.0 35.0 41.0 31.0 41.0 45 36.381352436074984 39.0 35.0 41.0 31.0 41.0 46 36.23706508520013 38.0 35.0 41.0 30.0 41.0 47 36.148164336334624 38.0 35.0 40.0 30.0 41.0 48 36.03205184241158 38.0 35.0 40.0 30.0 41.0 49 35.933840500695496 38.0 35.0 40.0 30.0 41.0 50 35.839423329085214 38.0 35.0 40.0 30.0 41.0 51 34.1993281221005 36.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 23.0 9 49.0 10 54.0 11 41.0 12 23.0 13 34.0 14 55.0 15 71.0 16 114.0 17 185.0 18 326.0 19 576.0 20 929.0 21 1450.0 22 2132.0 23 3140.0 24 4461.0 25 6245.0 26 8080.0 27 9629.0 28 10904.0 29 12663.0 30 14919.0 31 19198.0 32 24529.0 33 35977.0 34 74226.0 35 99832.0 36 88708.0 37 143219.0 38 266294.0 39 475740.0 40 278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.87643182379346 21.54150046085211 25.610571533295605 11.97149618205882 2 32.15720194524065 25.085000759140748 26.038910947422984 16.71888634819562 3 32.716052222748765 24.152177813766674 26.390876201781143 16.740893761703422 4 29.376293031394685 25.720225196418085 25.688172587197666 19.21530918498956 5 25.931327668149674 31.1179459338428 23.381995021877056 19.56873137613047 6 27.041666858368874 35.39551232798561 22.75029790523163 14.812522908413886 7 82.6163670744556 6.196045413486327 6.899439156019525 4.288148356038543 8 83.85330640300712 5.256474550381641 6.594249240475851 4.295969806135391 9 76.63280438860032 7.93831176300086 8.92849200908515 6.500391839313675 10 41.04612661854175 27.320555230939814 15.502344134602556 16.130974015915882 11 32.71030115650108 24.737943081313944 22.095059757412358 20.456696004772617 12 28.550133194694293 22.81424976190586 27.88431308497929 20.751303958420557 13 25.62215034667427 23.301863498826016 29.579037286846315 21.496948867653394 14 21.995604651769103 26.635334857749292 28.59345789376017 22.77560259672143 15 21.850907824977416 25.2540054259393 30.70371580224307 22.191370946840212 16 24.07227633336554 23.69968392139903 29.39086239922215 22.837177346013284 17 23.956488199578867 24.124956100194307 27.18912419695945 24.72943150326737 18 24.658885090629138 24.09213668214087 27.954246050551106 23.294732176678888 19 25.801353570952056 25.83401962723889 25.456366276974418 22.90826052483464 20 26.788313219937642 24.997814594825883 26.064675724212606 22.149196461023873 21 26.817528636475867 25.561572448865356 26.08806339361984 21.532835521038933 22 24.937926824966684 24.155475091748677 25.40874744844361 25.497850634841036 23 24.300018556773757 25.35591431984823 26.133765200068094 24.210301923309917 24 24.713098475123953 24.441648148233348 27.28344168342144 23.56181169322126 25 24.295494384658916 26.015599958899045 25.36059185372968 24.328313802712355 26 23.147581561621525 26.36150742347631 25.81285570344742 24.678055311454745 27 23.87420961179536 25.13070256558899 26.883320834349355 24.111766988266293 28 22.56419340145663 26.01797706628142 27.1970990088229 24.220730523439045 29 23.97036743945661 25.925576601901994 26.103246208513724 24.000809750127676 30 24.620544648977923 25.265584239317967 26.645073329928703 23.468797781775407 31 25.37638811568998 26.59323705281626 24.137071679756094 23.893303151737666 32 25.21137085482315 27.238430004922915 24.16751399042716 23.38268514982678 33 25.279616840962316 26.054707209383288 24.456754282243928 24.208921667410472 34 23.24642322019836 26.630350600334634 25.578058838775377 24.54516734069163 35 23.701140858181773 26.15538920915938 25.836856819921078 24.306613112737768 36 23.762408883940417 27.87457461279988 24.57982709994433 23.783189403315376 37 23.74952649554561 28.235281487854515 25.424850433937117 22.59034158266276 38 24.07227633336554 27.75824971283009 24.402617578632412 23.766856375171958 39 24.764244624286675 25.611491703895233 25.211524216589755 24.412739455228333 40 23.93478750960428 26.01506319271593 27.13107676829951 22.91907252938028 41 21.41950117551794 27.00846403589892 26.348164949781687 25.22386983880145 42 21.65383795489017 26.583958665936663 26.283753007807647 25.47845037136552 43 23.6578161591159 24.22341435435463 27.218262932614373 24.900506553915097 44 23.61840218509845 24.559583346752486 26.455441505521794 25.36657296262727 45 24.327930398295845 24.771682669967014 25.72735651856521 25.173030413171936 46 23.413970950214168 26.52384085342756 25.713400597804164 24.348787598554107 47 22.73542181387096 25.810708638714953 27.62099093171874 23.83287861569535 48 22.563349911740303 25.097883147535555 27.179155682130133 25.159611258594012 49 22.866776166968023 25.16957977342333 27.36050597114038 24.603138088468267 50 21.66043251085418 24.779504120063862 28.23773527612019 25.322328092961772 51 22.378242259448236 24.43896431731776 26.73847064579106 26.444322777442935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 680.0 1 1582.5 2 2485.0 3 3910.0 4 5335.0 5 3880.5 6 2426.0 7 2217.0 8 2008.0 9 1977.5 10 1947.0 11 2060.0 12 2173.0 13 2314.5 14 2456.0 15 2511.5 16 2567.0 17 2454.5 18 2342.0 19 2390.0 20 2438.0 21 2812.5 22 3187.0 23 3646.5 24 4106.0 25 5008.5 26 7225.5 27 8540.0 28 11448.5 29 14357.0 30 17250.0 31 20143.0 32 21588.5 33 23034.0 34 27167.5 35 31301.0 36 33886.0 37 36471.0 38 38216.5 39 39962.0 40 45363.5 41 50765.0 42 56688.0 43 62611.0 44 69588.5 45 76566.0 46 85601.5 47 94637.0 48 102582.0 49 110527.0 50 108881.0 51 107235.0 52 97249.0 53 87263.0 54 80990.5 55 74718.0 56 73663.5 57 72609.0 58 70924.0 59 69239.0 60 68211.0 61 67183.0 62 63941.5 63 60700.0 64 54219.5 65 47739.0 66 40615.5 67 33492.0 68 29360.5 69 25229.0 70 21204.5 71 17180.0 72 15590.5 73 14001.0 74 11316.5 75 6660.0 76 4688.0 77 3763.5 78 2839.0 79 1975.5 80 1112.0 81 845.5 82 579.0 83 481.0 84 383.0 85 272.5 86 162.0 87 118.5 88 75.0 89 53.0 90 31.0 91 25.5 92 20.0 93 17.5 94 15.0 95 9.0 96 3.0 97 3.5 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1304106.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.60364878791331 #Duplication Level Percentage of deduplicated Percentage of total 1 72.17064822710742 17.756612817757894 2 10.05609705248747 4.94833360113144 3 3.7127228818767746 2.740395894976366 4 1.837231439013351 1.8081038827038824 5 1.1248401534684633 1.383758603924029 6 0.8158218599040108 1.2043316708748302 7 0.5973109391771697 1.0287219995285612 8 0.5103447354580838 1.004507410557698 9 0.4099008312072162 0.9076550480006474 >10 7.810924160165987 49.307167107342885 >50 0.878476054003291 13.046088356941702 >100 0.06880151466457227 3.0532154102629128 >500 0.004691012363336159 0.8091432487512121 >1k 0.0021891391028902077 1.001964947245938 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2699 0.2069617040332611 No Hit CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC 2500 0.19170220825607737 RNA PCR Primer, Index 30 (95% over 24bp) GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC 2004 0.1536684901380716 Illumina Single End Adapter 2 (95% over 22bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 1752 0.13434490754585898 No Hit CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT 1458 0.11180072785494431 RNA PCR Primer, Index 30 (96% over 25bp) AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1331 0.10206225567553556 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.668088330243093E-5 0.0 0.0 0.12429971183324055 0.0 2 7.668088330243093E-5 0.0 0.0 0.6142138752524718 0.0 3 7.668088330243093E-5 0.0 0.0 0.8944058228395545 0.0 4 7.668088330243093E-5 0.0 0.0 1.1849496896724652 0.0 5 7.668088330243093E-5 0.0 0.0 2.019467742652821 0.0 6 7.668088330243093E-5 0.0 0.0 2.629234126673752 0.0 7 7.668088330243093E-5 0.0 0.0 3.1525044743295405 0.0 8 7.668088330243093E-5 0.0 0.0 3.9410906782117405 0.0 9 7.668088330243093E-5 0.0 0.0 4.265297452814418 0.0 10 7.668088330243093E-5 0.0 0.0 4.938555608209762 0.0 11 7.668088330243093E-5 0.0 0.0 5.859262974022051 0.0 12 7.668088330243093E-5 0.0 0.0 6.5906452389606365 0.0 13 7.668088330243093E-5 0.0 0.0 6.885253192608577 0.0 14 7.668088330243093E-5 0.0 0.0 7.01185333094089 0.0 15 7.668088330243093E-5 0.0 0.0 7.2586124134081125 0.0 16 7.668088330243093E-5 0.0 0.0 7.756884793107309 0.0 17 7.668088330243093E-5 0.0 0.0 8.383444290571472 0.0 18 7.668088330243093E-5 0.0 0.0 9.055552232717279 0.0 19 1.5336176660486186E-4 0.0 0.0 9.451839037624243 0.0 20 1.5336176660486186E-4 0.0 0.0 9.845825416032133 0.0 21 1.5336176660486186E-4 0.0 0.0 10.330448598503496 0.0 22 1.5336176660486186E-4 0.0 0.0 10.833628554734048 0.0 23 1.5336176660486186E-4 0.0 0.0 11.34532008901117 0.0 24 1.5336176660486186E-4 0.0 0.0 11.768751926607193 0.0 25 1.5336176660486186E-4 0.0 0.0 12.166188944763693 0.0 26 2.3004264990729282E-4 0.0 0.0 12.508569088709047 0.0 27 2.3004264990729282E-4 0.0 0.0 12.887602694872962 0.0 28 2.3004264990729282E-4 0.0 0.0 13.277524986465824 0.0 29 2.3004264990729282E-4 0.0 0.0 13.686924222417503 0.0 30 2.3004264990729282E-4 0.0 0.0 14.151533694346933 0.0 31 2.3004264990729282E-4 0.0 0.0 14.541149262406584 0.0 32 2.3004264990729282E-4 0.0 0.0 14.95392245722357 0.0 33 2.3004264990729282E-4 0.0 0.0 15.360944585792872 0.0 34 2.3004264990729282E-4 0.0 0.0 15.757844837766255 0.0 35 2.3004264990729282E-4 0.0 0.0 16.18794791220959 0.0 36 2.3004264990729282E-4 0.0 0.0 16.578867055285382 0.0 37 2.3004264990729282E-4 0.0 0.0 16.984815651488454 0.0 38 2.3004264990729282E-4 0.0 0.0 17.354877594305986 0.0 39 2.3004264990729282E-4 0.0 0.0 17.75476840072816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGAA 30 2.165727E-6 45.000004 28 CCGTCGA 20 7.033636E-4 45.0 35 ATCGTAG 25 3.8910955E-5 45.0 1 GTCGTAC 40 6.8157533E-9 45.0 45 TGCGTTA 20 7.033636E-4 45.0 34 GTACGAG 65 0.0 41.538464 1 CGAATAT 310 0.0 39.193546 14 GCTACGA 330 0.0 38.181816 10 CTATACG 65 9.094947E-12 38.076927 1 TATTGCG 125 0.0 37.8 1 GCGATAT 125 0.0 37.8 9 TCGACCC 30 1.1400061E-4 37.500004 38 AGTAACG 60 1.5643309E-10 37.500004 1 TATACGG 115 0.0 37.173912 2 TAGGGTA 370 0.0 37.094593 5 CTACGAA 335 0.0 36.9403 11 TCGACGT 55 2.750312E-9 36.81818 26 ATCTACG 50 4.879803E-8 36.0 1 TACCACG 25 0.0021072123 36.0 1 ATTACGA 25 0.0021072123 36.0 12 >>END_MODULE