SRR3549919_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768501
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2992	0.3893293567607589	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2690	0.3500320754299604	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	1955	0.2543913410652686	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.15770961911565504	No Hit
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	920	0.11971357226600876	Illumina PCR Primer Index 7 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	901	0.11724122675181944	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	786	0.10227703021856835	Illumina PCR Primer Index 7 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	20	7.031825E-4	45.000004	29
CACATCG	20	7.031825E-4	45.000004	17
TATGCGC	20	7.031825E-4	45.000004	1
TCGTAAG	20	7.031825E-4	45.000004	1
CGGTCTA	80	0.0	45.000004	31
TTCGATA	20	7.031825E-4	45.000004	28
CGACAGG	75	0.0	45.0	2
TGTTGCG	35	1.2112287E-7	45.0	1
ACGATTC	25	3.8895952E-5	45.0	36
CGTAGGC	25	3.8895952E-5	45.0	33
TTGCGTA	25	3.8895952E-5	45.0	36
ATAAGCG	25	3.8895952E-5	45.0	1
GTACGAG	35	1.2112287E-7	45.0	1
CAGACGA	25	3.8895952E-5	45.0	33
TCGTTCA	65	0.0	44.999996	16
GGTTACG	30	2.1645592E-6	44.999996	1
CCTATGC	60	3.6379788E-12	41.249996	35
TATGGGA	360	0.0	40.625	4
GTTAGCG	100	0.0	40.5	1
TACGGGA	225	0.0	40.0	4