##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768501
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55609947156868	34.0	31.0	34.0	31.0	34.0
2	32.72449352700907	34.0	31.0	34.0	31.0	34.0
3	32.73040633649143	34.0	31.0	34.0	31.0	34.0
4	36.15642399944828	37.0	35.0	37.0	35.0	37.0
5	36.16594383091239	37.0	35.0	37.0	35.0	37.0
6	36.14995165913903	37.0	36.0	37.0	35.0	37.0
7	36.25468021512008	37.0	37.0	37.0	35.0	37.0
8	36.24908230438217	37.0	37.0	37.0	35.0	37.0
9	37.61994324015193	39.0	38.0	39.0	35.0	39.0
10	37.649965322101075	39.0	37.0	39.0	35.0	39.0
11	37.625617923724235	39.0	37.0	39.0	35.0	39.0
12	37.498027979143814	39.0	37.0	39.0	35.0	39.0
13	37.42392267544219	39.0	37.0	39.0	35.0	39.0
14	38.653010210786974	40.0	38.0	41.0	35.0	41.0
15	38.70992880946154	40.0	38.0	41.0	35.0	41.0
16	38.73523782011995	40.0	38.0	41.0	35.0	41.0
17	38.68704269740703	40.0	38.0	41.0	35.0	41.0
18	38.64558927054096	40.0	38.0	41.0	35.0	41.0
19	38.63141752580673	40.0	38.0	41.0	35.0	41.0
20	38.58063034400736	40.0	38.0	41.0	35.0	41.0
21	38.55611118267901	40.0	38.0	41.0	35.0	41.0
22	38.5017846430909	40.0	38.0	41.0	35.0	41.0
23	38.476067044805404	40.0	37.0	41.0	35.0	41.0
24	38.46132145566499	40.0	37.0	41.0	35.0	41.0
25	38.36217649684256	40.0	37.0	41.0	34.0	41.0
26	38.27594238654211	40.0	37.0	41.0	34.0	41.0
27	38.18451895313083	40.0	37.0	41.0	34.0	41.0
28	38.08109293286541	40.0	37.0	41.0	34.0	41.0
29	37.9717970438555	40.0	36.0	41.0	34.0	41.0
30	37.86877310504475	40.0	36.0	41.0	34.0	41.0
31	37.73842584459877	40.0	36.0	41.0	33.0	41.0
32	37.599572414349495	40.0	36.0	41.0	33.0	41.0
33	37.5017573171668	40.0	36.0	41.0	33.0	41.0
34	37.39278803801166	40.0	35.0	41.0	33.0	41.0
35	37.28113040841847	40.0	35.0	41.0	32.0	41.0
36	37.18754692576848	40.0	35.0	41.0	32.0	41.0
37	37.036998000002605	39.0	35.0	41.0	32.0	41.0
38	36.88378154355036	39.0	35.0	41.0	31.0	41.0
39	36.77545117052548	39.0	35.0	41.0	31.0	41.0
40	36.693049195772026	39.0	35.0	41.0	31.0	41.0
41	36.64155674488387	39.0	35.0	41.0	31.0	41.0
42	36.53552565318718	39.0	35.0	41.0	31.0	41.0
43	36.454349441314974	39.0	35.0	41.0	31.0	41.0
44	36.308313196729735	39.0	35.0	41.0	31.0	41.0
45	36.13813905251912	38.0	35.0	40.0	30.0	41.0
46	36.002590757851976	38.0	35.0	40.0	30.0	41.0
47	35.89408471817213	38.0	35.0	40.0	30.0	41.0
48	35.741725775242976	38.0	35.0	40.0	29.0	41.0
49	35.60407078195084	37.0	35.0	40.0	29.0	41.0
50	35.4489831503147	37.0	35.0	40.0	29.0	41.0
51	33.68129124100034	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	33.0
10	36.0
11	27.0
12	18.0
13	24.0
14	20.0
15	34.0
16	61.0
17	124.0
18	192.0
19	351.0
20	556.0
21	897.0
22	1446.0
23	2182.0
24	3223.0
25	4039.0
26	5123.0
27	5980.0
28	6974.0
29	8203.0
30	9805.0
31	12440.0
32	15760.0
33	23056.0
34	44822.0
35	63330.0
36	54249.0
37	88040.0
38	162290.0
39	255042.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07333237041981	19.862043120308236	24.69821119295876	11.366413316313187
2	32.07946378729501	25.162231408937657	25.150520298607287	17.607784505160044
3	32.70587806652171	24.689362798486925	25.568606937401512	17.036152197589853
4	29.2379580508028	25.716036804116065	25.52865903883014	19.517346106250997
5	25.40673336794617	30.857344362596795	24.017145065523664	19.71877720393337
6	26.412977992221222	35.16872456899861	23.992291486933652	14.426005951846516
7	80.53079956955163	6.654252889716473	8.012871811487559	4.802075729244334
8	81.07354447164025	5.864793930001393	7.684049858100379	5.377611740257983
9	73.98936370935107	8.114498224465551	10.399986467161396	7.496151599021992
10	39.428055396154335	26.701591800140793	18.02912423015715	15.841228573547724
11	31.20061001872476	25.176805235126565	23.007907601941962	20.61467714420671
12	27.494173722610643	22.24837703529338	29.47283087465078	20.784618367445194
13	25.193591159933426	24.624170950981195	30.294170079154092	19.888067809931282
14	20.68585467032574	28.56964402128299	28.647457843255896	22.097043465135375
15	20.277657413588273	25.15182153308844	33.87516737128514	20.69535368203815
16	21.77785064690872	24.747788226690663	30.33763131082458	23.136729815576036
17	21.767440771059505	23.811550017501602	27.787341851214247	26.633667360224646
18	22.886242177954223	24.834450443135403	29.3131694038134	22.966137975096977
19	24.72033217913835	25.83640099362265	26.76652340075029	22.67674342648871
20	25.484156819574732	25.30940102875598	26.744662661466933	22.461779490202353
21	24.353514178901527	26.034188634757793	28.008811959906367	21.60348522643432
22	22.528532819085466	24.94115167058989	26.228723189690058	26.301592320634587
23	21.92736248879312	26.162360231151293	26.961057955682556	24.949219324373033
24	22.76913107465052	25.098080549016856	27.922409990357856	24.210378385974774
25	21.586829425075567	27.14700436303922	26.214149363501154	25.052016848384064
26	22.01155235972367	26.570947858233108	27.300289784918952	24.117209997124274
27	24.48728108356398	26.06112418851765	26.553251069289434	22.898343658628942
28	22.613893801049056	25.439654600319322	28.553899084061047	23.39255251457057
29	23.21129055134606	24.649805270259893	27.771336667096076	24.36756751129797
30	25.04772277459626	25.525145705731028	25.611677798727655	23.815453720945058
31	25.837702228103804	26.85240487650634	24.152863821907843	23.157029073482015
32	25.297169424633147	26.51005008451518	25.447982500998695	22.744797989852973
33	24.688191687453887	25.904715803883143	24.630677123386956	24.776415385276014
34	22.782273542910158	26.038482708545597	26.787343152448727	24.391900596095518
35	24.544665524182793	24.111094195062858	27.079079923123068	24.265160357631284
36	26.72787673666007	25.887799755628166	25.104977091766962	22.279346415944808
37	23.70615002452827	27.207902136757138	27.715123337510295	21.370824501204293
38	22.658526143752578	27.031194494216663	26.290401704096677	24.01987765793408
39	22.59307404935062	25.671274337964427	27.4904001426153	24.245251470069658
40	23.493658433756103	24.357027512000634	28.91564226982138	23.23367178442188
41	21.125151431162745	26.383570092947178	26.972899189461042	25.51837928642903
42	23.06112809222109	25.72709729720586	27.367693731042642	23.84408087953041
43	24.73256378326118	23.917730751163628	27.722280127156633	23.627425338418558
44	23.28468017608305	24.252798630060337	27.142319918907067	25.320201274949543
45	23.019228341927985	23.88689149396032	27.252664602908776	25.84121556120291
46	23.935817910451647	24.75624625081815	27.362098422773684	23.945837415956518
47	22.028078037634305	25.022999319454364	29.975497754719903	22.973424888191428
48	22.479996772938488	24.30133467620732	29.036657076568538	24.182011474285655
49	23.07531154806565	23.460607077934835	29.6037350634547	23.860346310544813
50	21.974076806666485	22.862299463501024	29.71746295710741	25.446160772725086
51	21.76613953657835	23.40465399524529	27.482332488832157	27.346873979344206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	545.0
1	959.5
2	1374.0
3	1980.5
4	2587.0
5	2034.0
6	1481.0
7	1389.0
8	1297.0
9	1337.0
10	1377.0
11	1466.5
12	1556.0
13	1525.5
14	1495.0
15	1544.5
16	1594.0
17	1568.5
18	1543.0
19	1657.0
20	1771.0
21	1933.5
22	2096.0
23	2404.5
24	2713.0
25	3167.0
26	4316.5
27	5012.0
28	6868.5
29	8725.0
30	10172.0
31	11619.0
32	12407.0
33	13195.0
34	14541.0
35	15887.0
36	17640.0
37	19393.0
38	20622.5
39	21852.0
40	24976.5
41	28101.0
42	31861.5
43	35622.0
44	43305.5
45	50989.0
46	59213.0
47	67437.0
48	74126.0
49	80815.0
50	78860.0
51	76905.0
52	67393.5
53	57882.0
54	51373.5
55	44865.0
56	41874.5
57	38884.0
58	37313.5
59	35743.0
60	33532.0
61	31321.0
62	29694.5
63	28068.0
64	24609.0
65	21150.0
66	18364.0
67	15578.0
68	13177.5
69	10777.0
70	9439.5
71	8102.0
72	7011.5
73	5921.0
74	4867.0
75	3147.5
76	2482.0
77	2095.5
78	1709.0
79	1246.5
80	784.0
81	588.0
82	392.0
83	328.0
84	264.0
85	175.0
86	86.0
87	66.0
88	46.0
89	30.0
90	14.0
91	12.5
92	11.0
93	8.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	768501.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.815673493890674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39075622822037	19.8578196572612
2	11.427109752891088	6.357055077305445
3	4.393524441145975	3.6662652402703477
4	2.2948951071560466	2.553362120135194
5	1.3530999299647513	1.8818692928252927
6	0.8557566104688323	1.4282067880223774
7	0.6207795712333211	1.2087181305572454
8	0.4773758560946642	1.0622824757596576
9	0.4302629785924922	1.077124907613387
>10	5.884564306573142	41.86302678374168
>50	0.7951803118461246	13.466225480389916
>100	0.07057813418395009	3.6198390949680914
>500	0.0042346880510370066	0.8000606113073954
>1k	0.0018820835782386695	1.1581443398428173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2992	0.3893293567607589	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2690	0.3500320754299604	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	1955	0.2543913410652686	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.15770961911565504	No Hit
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	920	0.11971357226600876	Illumina PCR Primer Index 7 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	901	0.11724122675181944	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG	786	0.10227703021856835	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6024689623045384E-4	0.0	0.0	0.2765123272448572	0.0
2	2.6024689623045384E-4	0.0	0.0	1.3319436149074626	0.0
3	2.6024689623045384E-4	0.0	0.0	1.8199065453395638	0.0
4	2.6024689623045384E-4	0.0	0.0	2.34261243641843	0.0
5	2.6024689623045384E-4	0.0	0.0	3.763560489836708	0.0
6	2.6024689623045384E-4	0.0	0.0	4.548725375763987	0.0
7	2.6024689623045384E-4	0.0	0.0	5.3578329761444685	0.0
8	3.9037034434568073E-4	0.0	0.0	6.705521528273874	0.0
9	3.9037034434568073E-4	0.0	0.0	7.245143467607719	0.0
10	3.9037034434568073E-4	0.0	0.0	8.473899188159807	0.0
11	3.9037034434568073E-4	0.0	0.0	9.982160075263403	0.0
12	3.9037034434568073E-4	0.0	0.0	11.276107643321218	0.0
13	3.9037034434568073E-4	0.0	0.0	11.787883164758407	0.0
14	3.9037034434568073E-4	0.0	0.0	11.985540682445436	0.0
15	3.9037034434568073E-4	0.0	0.0	12.376171273687348	0.0
16	3.9037034434568073E-4	0.0	0.0	13.190093441648091	0.0
17	3.9037034434568073E-4	0.0	0.0	14.182805227319157	0.0
18	3.9037034434568073E-4	0.0	0.0	15.378509592049978	0.0
19	3.9037034434568073E-4	0.0	0.0	15.993342884394425	0.0
20	3.9037034434568073E-4	0.0	0.0	16.578117660224255	0.0
21	3.9037034434568073E-4	0.0	0.0	17.31760921586309	0.0
22	3.9037034434568073E-4	0.0	0.0	18.03576052601103	0.0
23	3.9037034434568073E-4	0.0	0.0	18.765102452696873	0.0
24	3.9037034434568073E-4	0.0	0.0	19.39932413881049	0.0
25	3.9037034434568073E-4	0.0	0.0	19.914743116794902	0.0
26	3.9037034434568073E-4	0.0	0.0	20.395028763788204	0.0
27	3.9037034434568073E-4	0.0	0.0	20.881039842498577	0.0
28	5.204937924609077E-4	0.0	0.0	21.385398327393197	0.0
29	5.204937924609077E-4	0.0	0.0	21.932307179821496	0.0
30	5.204937924609077E-4	0.0	0.0	22.552865903883013	0.0
31	5.204937924609077E-4	0.0	0.0	23.092878213561207	0.0
32	5.204937924609077E-4	0.0	0.0	23.6295073135884	0.0
33	5.204937924609077E-4	0.0	0.0	24.142193699162394	0.0
34	6.506172405761346E-4	0.0	0.0	24.690403786071847	0.0
35	6.506172405761346E-4	0.0	0.0	25.24147658883983	0.0
36	6.506172405761346E-4	0.0	0.0	25.744663962701416	0.0
37	6.506172405761346E-4	0.0	0.0	26.230544917963673	0.0
38	6.506172405761346E-4	0.0	0.0	26.70471476289556	0.0
39	6.506172405761346E-4	0.0	0.0	27.22846164155935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	20	7.031825E-4	45.000004	29
CACATCG	20	7.031825E-4	45.000004	17
TATGCGC	20	7.031825E-4	45.000004	1
TCGTAAG	20	7.031825E-4	45.000004	1
CGGTCTA	80	0.0	45.000004	31
TTCGATA	20	7.031825E-4	45.000004	28
CGACAGG	75	0.0	45.0	2
TGTTGCG	35	1.2112287E-7	45.0	1
ACGATTC	25	3.8895952E-5	45.0	36
CGTAGGC	25	3.8895952E-5	45.0	33
TTGCGTA	25	3.8895952E-5	45.0	36
ATAAGCG	25	3.8895952E-5	45.0	1
GTACGAG	35	1.2112287E-7	45.0	1
CAGACGA	25	3.8895952E-5	45.0	33
TCGTTCA	65	0.0	44.999996	16
GGTTACG	30	2.1645592E-6	44.999996	1
CCTATGC	60	3.6379788E-12	41.249996	35
TATGGGA	360	0.0	40.625	4
GTTAGCG	100	0.0	40.5	1
TACGGGA	225	0.0	40.0	4
>>END_MODULE