FastQCFastQC Report
Sat 18 Jun 2016
SRR3549917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502812
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.27315459285659155No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC33930.22577674386416932No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC28740.19124148596098514No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT22100.14705764926018688Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG16580.11032650790651125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG453.8562575E-1045.0000042
TCGTGAT207.033979E-445.00000426
ATAACGG1150.043.043482
TAATCGT1350.041.66666821
TCGGACG501.0822987E-940.51
TTACGAG501.0822987E-940.51
TATCGAC501.0822987E-940.515
CGGTCTA1300.039.8076931
GCGTTAG850.039.7058831
TTATGCG1050.038.5714261
ATAGTCG356.249571E-638.5714261
TGATTCG2200.037.8409115
TACGGGA5950.037.8151254
CGTTTTT19550.037.519181
CGCATCG850.037.05882321
CGATTCG850.037.05882310
TTACGCG850.037.0588231
CACGACG1400.036.96428326
TAACGGG4350.036.724143
ATAACGC800.036.56250411
Produced by FastQC (version 0.11.5)