##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502812
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.674130895947066	34.0	31.0	34.0	31.0	34.0
2	32.85879670910267	34.0	31.0	34.0	31.0	34.0
3	32.83068873551715	34.0	31.0	34.0	31.0	34.0
4	36.24451628014682	37.0	37.0	37.0	35.0	37.0
5	36.25646654405208	37.0	37.0	37.0	35.0	37.0
6	36.25324791124905	37.0	37.0	37.0	35.0	37.0
7	36.34746595049813	37.0	37.0	37.0	35.0	37.0
8	36.347021450454214	37.0	37.0	37.0	35.0	37.0
9	37.78513679688477	39.0	38.0	39.0	35.0	39.0
10	37.757945770994645	39.0	37.0	39.0	35.0	39.0
11	37.73522236979742	39.0	37.0	39.0	35.0	39.0
12	37.67074058498335	39.0	37.0	39.0	35.0	39.0
13	37.664165577597196	39.0	37.0	39.0	35.0	39.0
14	38.98595765804372	40.0	38.0	41.0	35.0	41.0
15	39.00485489868327	40.0	38.0	41.0	35.0	41.0
16	39.00389137164196	40.0	38.0	41.0	35.0	41.0
17	38.95558459740806	40.0	38.0	41.0	35.0	41.0
18	38.89815359472775	40.0	38.0	41.0	35.0	41.0
19	38.86098593836088	40.0	38.0	41.0	35.0	41.0
20	38.825167752187234	40.0	38.0	41.0	35.0	41.0
21	38.799576394119825	40.0	38.0	41.0	35.0	41.0
22	38.771656734175664	40.0	38.0	41.0	35.0	41.0
23	38.74736028192482	40.0	38.0	41.0	35.0	41.0
24	38.727552747782156	40.0	38.0	41.0	35.0	41.0
25	38.65923615196046	40.0	38.0	41.0	35.0	41.0
26	38.58795644431905	40.0	38.0	41.0	35.0	41.0
27	38.51856186934893	40.0	38.0	41.0	35.0	41.0
28	38.42456541470257	40.0	38.0	41.0	35.0	41.0
29	38.35602191092432	40.0	38.0	41.0	34.0	41.0
30	38.25618573713811	40.0	37.0	41.0	34.0	41.0
31	38.161760087090066	40.0	37.0	41.0	34.0	41.0
32	38.06416238358491	40.0	37.0	41.0	34.0	41.0
33	37.92138271453781	40.0	37.0	41.0	34.0	41.0
34	37.80285158755719	40.0	37.0	41.0	33.0	41.0
35	37.74374239758533	40.0	37.0	41.0	33.0	41.0
36	37.583451556149406	40.0	36.0	41.0	33.0	41.0
37	37.47972933407505	40.0	36.0	41.0	33.0	41.0
38	37.35987069573573	40.0	36.0	41.0	33.0	41.0
39	37.26977625943897	40.0	35.0	41.0	33.0	41.0
40	37.203595659337296	40.0	35.0	41.0	32.0	41.0
41	37.09813203514478	39.0	35.0	41.0	32.0	41.0
42	36.9376868164481	39.0	35.0	41.0	32.0	41.0
43	36.882300647053654	39.0	35.0	41.0	31.0	41.0
44	36.81722131577337	39.0	35.0	41.0	31.0	41.0
45	36.73282020638643	39.0	35.0	41.0	31.0	41.0
46	36.62551004383782	39.0	35.0	41.0	31.0	41.0
47	36.53985861172256	39.0	35.0	41.0	31.0	41.0
48	36.389107220330956	39.0	35.0	41.0	31.0	41.0
49	36.29310519213315	39.0	35.0	40.0	31.0	41.0
50	36.202712648022505	38.0	35.0	40.0	30.0	41.0
51	34.54333609260506	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	24.0
9	42.0
10	67.0
11	53.0
12	24.0
13	26.0
14	33.0
15	59.0
16	78.0
17	196.0
18	284.0
19	558.0
20	956.0
21	1361.0
22	2102.0
23	3266.0
24	4791.0
25	6648.0
26	8346.0
27	9949.0
28	11558.0
29	13325.0
30	16002.0
31	20034.0
32	26348.0
33	38011.0
34	77003.0
35	101886.0
36	97340.0
37	157687.0
38	314180.0
39	590266.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.085902960583226	20.791423012326227	25.12257022169107	12.000103805399478
2	31.637423709685574	24.639542404505686	25.94210054218359	17.78093334362515
3	31.987700391000338	23.676680782426544	27.490531084393787	16.845087742179327
4	28.385919196812377	25.934381679145496	26.529798803842397	19.14990032019973
5	25.38487848114069	30.426094548087185	25.04065711479546	19.148369855976664
6	26.320923708354737	35.44987663127523	23.38742304426635	14.841776616103678
7	82.36459384141197	5.8023891211941345	7.272699446105035	4.560317591288864
8	84.0096432554438	4.655805250423873	7.073539471337733	4.261012022794601
9	77.40921685480286	6.979049941043856	9.321591789259069	6.290141414894211
10	38.70204656337586	26.80801058282739	17.66475114651733	16.82519170727942
11	29.14829000566937	26.340087782104483	23.873911041434322	20.63771117079182
12	26.106725259047703	22.921563043148442	30.025578715102085	20.946132982701762
13	24.807427675584172	23.53155284892588	31.043470507289	20.617548968200946
14	21.353835343343015	26.938565835247523	29.66159439770244	22.046004423707025
15	20.75222981983109	25.295978472357156	32.57413435612705	21.37765735168471
16	23.065892473576202	24.39972531494292	29.93408357133161	22.600298640149266
17	22.78076033462602	24.728908206748414	28.274461476219248	24.215869982406314
18	23.72598834717849	24.542990074606806	29.203919053081822	22.527102525132882
19	24.31208960269149	26.10566058828383	27.011163072959228	22.571086736065457
20	25.357330125125433	25.438577812793618	27.154694000314077	22.049398061766876
21	24.624969723425153	26.01516357335448	27.998778290298453	21.36108841292191
22	23.83897653199469	23.9781822343713	26.579638703976276	25.603202529657736
23	22.93347404731929	25.49893133672076	27.371487584608055	24.196107031351893
24	23.069951530863474	24.721721679092262	28.92943362177039	23.278893168273875
25	23.47991631687796	26.05834928121415	26.479027316790127	23.982707085117767
26	22.35302885523938	26.575912356302716	26.908488886168065	24.16256990228984
27	23.682802639318822	24.94929505487047	27.89756802580762	23.470334280003087
28	21.790084188840652	25.31254741111995	28.6477616628028	24.249606737236594
29	22.36660340747878	25.790850751790646	27.562396360955326	24.280149479775247
30	24.480374125306426	25.051836157816147	27.602853849982566	22.86493586689486
31	25.49547115673817	26.343747587855297	25.58144332092105	22.579337934485487
32	24.49408176139131	26.099804899082518	26.51329640700234	22.89281693252383
33	24.982299848550586	25.514435604719687	26.290447507738822	23.212817038990906
34	23.48484041916088	24.848417499993346	28.384654900280275	23.2820871805655
35	24.987623202369956	24.524890671620938	27.436565585049895	23.050920540959215
36	25.205281831659583	25.966987221289155	27.4963867735951	21.33134417345616
37	24.02835484411889	26.68217980692196	28.124808691972113	21.164656656987034
38	23.777159085767217	26.96831007471327	27.23966803565582	22.014862803863693
39	24.48729448527161	25.477904089134235	27.230618334162887	22.804183091431263
40	25.414423094838213	23.89680146285763	28.767736749506923	21.921038692797236
41	23.13702578898758	24.665959547834326	28.23985967639332	23.957154986784772
42	22.75301235284254	25.926396648416432	28.648626707798446	22.67196429094258
43	23.58006191060492	23.92993934038323	29.282039270381127	23.207959478630727
44	23.14820483200826	24.320873136493454	28.394835814459825	24.136086217038457
45	23.862865082259123	24.110201409091754	27.67278941078458	24.354144097864534
46	24.58378027324775	24.411835944881993	28.13073092309617	22.873652858774086
47	23.376310543168408	24.713803190285944	29.42164422429419	22.48824204225146
48	22.26246529838729	24.684591286202135	29.395825958270226	23.657117457140348
49	22.990966268568524	23.81415639481186	29.945928033579715	23.2489493030399
50	22.505210232550713	23.567418945283908	29.74430600767095	24.18306481449443
51	22.91005129051405	23.231581861204194	28.243652565989624	25.614714282292127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	663.0
1	1409.0
2	2155.0
3	4714.5
4	7274.0
5	5123.0
6	2972.0
7	2623.5
8	2275.0
9	2242.0
10	2209.0
11	2335.5
12	2462.0
13	2493.5
14	2525.0
15	2580.0
16	2635.0
17	2512.0
18	2389.0
19	2605.0
20	2821.0
21	3350.5
22	3880.0
23	4707.0
24	5534.0
25	6916.0
26	9887.5
27	11477.0
28	14541.0
29	17605.0
30	21246.0
31	24887.0
32	27723.0
33	30559.0
34	33479.5
35	36400.0
36	40957.0
37	45514.0
38	50078.5
39	54643.0
40	59544.5
41	64446.0
42	69860.0
43	75274.0
44	84741.5
45	94209.0
46	106686.0
47	119163.0
48	130387.5
49	141612.0
50	139211.0
51	136810.0
52	121646.5
53	106483.0
54	96059.0
55	85635.0
56	80624.0
57	75613.0
58	73543.0
59	71473.0
60	67942.5
61	64412.0
62	60063.5
63	55715.0
64	50534.5
65	45354.0
66	38199.0
67	31044.0
68	26155.5
69	21267.0
70	19176.5
71	17086.0
72	14536.5
73	11987.0
74	9450.5
75	5607.5
76	4301.0
77	3281.0
78	2261.0
79	1772.0
80	1283.0
81	1002.0
82	721.0
83	482.5
84	244.0
85	195.0
86	146.0
87	111.5
88	77.0
89	61.5
90	46.0
91	40.5
92	35.0
93	23.0
94	11.0
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1502812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.211534868394708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24159458089456	17.961099458562625
2	10.616792153461496	5.353312511349877
3	4.025310673065905	3.0445278117036736
4	1.9693568504786634	1.9860203561661922
5	1.1966941666452826	1.5085248354591045
6	0.8432848394596718	1.2756303080415687
7	0.6250604405504462	1.1031113164254214
8	0.5047554731550071	1.0180528169168426
9	0.46126388891757925	1.0466253557079201
>10	7.729552327190421	49.69000781067572
>50	0.715653651012239	10.863245623781694
>100	0.06191226529656009	2.7847719485939577
>500	0.006908664747759587	1.2666093244379497
>1k	0.001860025124396812	1.0984605221774506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4105	0.27315459285659155	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	3393	0.22577674386416932	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	2874	0.19124148596098514	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	2210	0.14705764926018688	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	1658	0.11032650790651125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3308384548433203E-4	0.0	0.0	0.16216266572265858	0.0
2	1.3308384548433203E-4	0.0	0.0	0.7590437127198878	0.0
3	1.3308384548433203E-4	0.0	0.0	1.1556335722631972	0.0
4	1.3308384548433203E-4	0.0	0.0	1.5131633231568553	0.0
5	1.3308384548433203E-4	0.0	0.0	2.5224712073100295	0.0
6	1.3308384548433203E-4	0.0	0.0	3.3273623047992698	0.0
7	1.3308384548433203E-4	0.0	0.0	3.9965744218172334	0.0
8	1.3308384548433203E-4	0.0	0.0	5.0426799892468255	0.0
9	1.3308384548433203E-4	0.0	0.0	5.509804286896831	0.0
10	1.3308384548433203E-4	0.0	0.0	6.397673162045552	0.0
11	1.3308384548433203E-4	0.0	0.0	7.51923726986476	0.0
12	1.3308384548433203E-4	0.0	0.0	8.372238177496586	0.0
13	1.3308384548433203E-4	0.0	0.0	8.734891656441391	0.0
14	1.3308384548433203E-4	0.0	0.0	8.894725354868074	0.0
15	1.3308384548433203E-4	0.0	0.0	9.17506647538082	0.0
16	1.3308384548433203E-4	0.0	0.0	9.759836892438974	0.0
17	1.3308384548433203E-4	0.0	0.0	10.451872888957501	0.0
18	1.9962576822649805E-4	0.0	0.0	11.221896018929845	0.0
19	1.9962576822649805E-4	0.0	0.0	11.645302273338249	0.0
20	1.9962576822649805E-4	0.0	0.0	12.090933529942534	0.0
21	1.9962576822649805E-4	0.0	0.0	12.631719736068117	0.0
22	1.9962576822649805E-4	0.0	0.0	13.206309238946721	0.0
23	1.9962576822649805E-4	0.0	0.0	13.800195899420553	0.0
24	1.9962576822649805E-4	0.0	0.0	14.299792655368735	0.0
25	1.9962576822649805E-4	0.0	0.0	14.730185811665065	0.0
26	1.9962576822649805E-4	0.0	0.0	15.120720356238838	0.0
27	1.9962576822649805E-4	0.0	0.0	15.514848164640688	0.0
28	1.9962576822649805E-4	0.0	0.0	15.926809208337437	0.0
29	1.9962576822649805E-4	0.0	0.0	16.36458851805815	0.0
30	1.9962576822649805E-4	0.0	0.0	16.91914890219136	0.0
31	1.9962576822649805E-4	0.0	0.0	17.36305006880435	0.0
32	1.9962576822649805E-4	0.0	0.0	17.799498540070214	0.0
33	1.9962576822649805E-4	0.0	0.0	18.2149863056723	0.0
34	1.9962576822649805E-4	0.0	0.0	18.63985648238103	0.0
35	1.9962576822649805E-4	0.0	0.0	19.095335943551156	0.0
36	1.9962576822649805E-4	0.0	0.0	19.50496801995193	0.0
37	1.9962576822649805E-4	0.0	0.0	19.913069632129634	0.0
38	1.9962576822649805E-4	0.0	0.0	20.328823565422688	0.0
39	1.9962576822649805E-4	0.0	0.0	20.755224206354487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	45	3.8562575E-10	45.000004	2
TCGTGAT	20	7.033979E-4	45.000004	26
ATAACGG	115	0.0	43.04348	2
TAATCGT	135	0.0	41.666668	21
TCGGACG	50	1.0822987E-9	40.5	1
TTACGAG	50	1.0822987E-9	40.5	1
TATCGAC	50	1.0822987E-9	40.5	15
CGGTCTA	130	0.0	39.80769	31
GCGTTAG	85	0.0	39.705883	1
TTATGCG	105	0.0	38.571426	1
ATAGTCG	35	6.249571E-6	38.571426	1
TGATTCG	220	0.0	37.84091	15
TACGGGA	595	0.0	37.815125	4
CGTTTTT	1955	0.0	37.51918	1
CGCATCG	85	0.0	37.058823	21
CGATTCG	85	0.0	37.058823	10
TTACGCG	85	0.0	37.058823	1
CACGACG	140	0.0	36.964283	26
TAACGGG	435	0.0	36.72414	3
ATAACGC	80	0.0	36.562504	11
>>END_MODULE