FastQCFastQC Report
Sat 18 Jun 2016
SRR3549914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524906
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC44670.8510095140844266Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC40560.7727097804178272Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT27860.5307616982850263TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC17110.32596312482615936Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC13070.24899696326580378TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG8250.1571709982358746TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG7560.14402578747432873No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG6640.12649883979226756TruSeq Adapter, Index 27 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG6530.12440322648245591TruSeq Adapter, Index 27 (95% over 22bp)
AGGCAAGGTGTAAATTTTTTTTATTATTTTAAAGATATGATTTATCCTGAG6190.11792587625212894No Hit
GGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG5970.11373464963250564No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC5760.10973393331377428No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.10535219639325898No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5430.10344709338433929No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.10154199037541961No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.0297794E-445.0000041
CTCGTGC302.1632386E-645.00000445
CAACGAT207.0297794E-445.0000043
CCGATCA207.0297794E-445.00000412
CGTGCCT207.0297794E-445.00000423
GTGATAA207.0297794E-445.00000415
ATTCGCA406.8030204E-945.00000439
AGTGCTC207.0297794E-445.00000412
ACGATAT207.0297794E-445.00000439
CCGTAAA207.0297794E-445.0000041
TACGATA207.0297794E-445.00000438
CTACGAT207.0297794E-445.00000437
TCTCGGA207.0297794E-445.00000413
AGTCTAT207.0297794E-445.00000421
ATCCACT207.0297794E-445.00000421
ATCCACG207.0297794E-445.00000436
CATACAG207.0297794E-445.00000444
TATAGTG302.1632386E-645.0000041
TGTAGCG453.8380676E-1045.0000041
CGTTTTA207.0297794E-445.0000046
Produced by FastQC (version 0.11.5)