##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	524906
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.341346831623184	33.0	31.0	34.0	30.0	34.0
2	32.51620480619387	34.0	31.0	34.0	31.0	34.0
3	32.534295664366574	34.0	31.0	34.0	31.0	34.0
4	36.00003238675115	37.0	35.0	37.0	35.0	37.0
5	36.01737263433834	37.0	35.0	37.0	35.0	37.0
6	36.01021135212781	37.0	35.0	37.0	35.0	37.0
7	36.15726815848933	37.0	35.0	37.0	35.0	37.0
8	36.15796352108759	37.0	36.0	37.0	35.0	37.0
9	37.51406918572087	39.0	38.0	39.0	35.0	39.0
10	37.52893470449947	39.0	37.0	39.0	35.0	39.0
11	37.5092492751083	39.0	37.0	39.0	35.0	39.0
12	37.35367475319391	39.0	37.0	39.0	35.0	39.0
13	37.20557585548651	39.0	37.0	39.0	34.0	39.0
14	38.32682042118017	40.0	38.0	41.0	34.0	41.0
15	38.395257436569594	40.0	37.0	41.0	34.0	41.0
16	38.43553893459019	40.0	37.0	41.0	34.0	41.0
17	38.41012105024519	40.0	37.0	41.0	34.0	41.0
18	38.38164547557086	40.0	37.0	41.0	34.0	41.0
19	38.366667555714734	40.0	37.0	41.0	34.0	41.0
20	38.32225960457682	40.0	37.0	41.0	34.0	41.0
21	38.27819647708352	40.0	37.0	41.0	34.0	41.0
22	38.26009799849878	40.0	37.0	41.0	34.0	41.0
23	38.24813395160276	40.0	37.0	41.0	34.0	41.0
24	38.24497910101999	40.0	37.0	41.0	34.0	41.0
25	38.172019371087394	40.0	37.0	41.0	34.0	41.0
26	38.11380513844384	40.0	36.0	41.0	34.0	41.0
27	38.037090450480655	40.0	36.0	41.0	34.0	41.0
28	37.97324854354875	40.0	36.0	41.0	34.0	41.0
29	37.93748785496832	40.0	36.0	41.0	34.0	41.0
30	37.82126514080616	40.0	36.0	41.0	34.0	41.0
31	37.72175208513524	40.0	36.0	41.0	33.0	41.0
32	37.636748293980254	40.0	36.0	41.0	33.0	41.0
33	37.54146647209215	40.0	36.0	41.0	33.0	41.0
34	37.46581292650494	40.0	36.0	41.0	33.0	41.0
35	37.40509348340465	40.0	35.0	41.0	33.0	41.0
36	37.30836949853879	40.0	35.0	41.0	33.0	41.0
37	37.19504063584718	40.0	35.0	41.0	32.0	41.0
38	37.075518283273574	39.0	35.0	41.0	32.0	41.0
39	37.00224421134451	39.0	35.0	41.0	32.0	41.0
40	36.86922610905572	39.0	35.0	41.0	31.0	41.0
41	36.7800310912811	39.0	35.0	41.0	31.0	41.0
42	36.6551477788404	39.0	35.0	41.0	31.0	41.0
43	36.59287758189085	39.0	35.0	41.0	31.0	41.0
44	36.49514770263628	39.0	35.0	41.0	31.0	41.0
45	36.40772443065997	39.0	35.0	41.0	31.0	41.0
46	36.23939714920386	38.0	35.0	40.0	30.0	41.0
47	36.18326519414905	38.0	35.0	40.0	30.0	41.0
48	36.06777213443931	38.0	35.0	40.0	30.0	41.0
49	35.99627933382358	38.0	35.0	40.0	30.0	41.0
50	35.84365010116097	38.0	35.0	40.0	29.0	41.0
51	34.01517033526003	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	13.0
10	17.0
11	12.0
12	8.0
13	10.0
14	9.0
15	19.0
16	33.0
17	59.0
18	121.0
19	228.0
20	411.0
21	686.0
22	1021.0
23	1480.0
24	2195.0
25	2765.0
26	3432.0
27	3964.0
28	4646.0
29	5745.0
30	6974.0
31	8949.0
32	11619.0
33	16427.0
34	32546.0
35	46162.0
36	35042.0
37	54856.0
38	106627.0
39	178756.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31539742353869	22.107386846406783	25.040483438939543	11.53673229111498
2	31.176820230669872	25.40378658274053	25.067535901666204	18.351857284923394
3	30.948588890201297	24.761957379035486	27.222016894453482	17.067436836309742
4	29.129787047585666	25.93873950764518	25.344537879163127	19.58693556560603
5	23.860081614612902	30.750267666972753	25.450652116759954	19.938998601654394
6	24.744430431353422	33.787382883792525	25.189462494237063	16.278724190616987
7	76.3626249271298	6.902378711616937	10.224687848871989	6.510308512381265
8	74.99304637401745	5.893245647792176	10.328896983459895	8.784810994730485
9	68.41091547819991	8.165462006530694	14.144246779423364	9.279375735846038
10	37.43832229008623	25.04086445954133	20.971564432488865	16.549248817883583
11	29.78057023543263	24.15994482821686	25.179174937988897	20.88030999836161
12	26.896053769627326	19.903373175387593	32.31969152572079	20.880881529264286
13	23.440577932048786	25.95512339352189	31.664717111254205	18.93958156317512
14	18.09428735811745	29.478611408518855	29.56357900271668	22.86352223064701
15	16.45227907472957	24.8090134233558	38.817807378844975	19.920900123069654
16	17.548475346061963	25.43960251930822	32.55935348424289	24.452568650386926
17	17.588673019550168	24.608977607419234	28.276681920191425	29.525667452839176
18	19.485774595832396	24.473715293785936	32.81139861232297	23.2291114980587
19	20.926032470575684	26.52874990950761	28.039687105881818	24.50553051403489
20	22.756264931244832	26.18278320308779	28.662655789798553	22.398296075868824
21	21.5874842352726	27.777544931854464	29.456512213615387	21.178458619257544
22	18.632288447836373	26.237459659443786	26.495791627453297	28.634460265266544
23	18.39167393780982	26.762506048702058	28.091696418025325	26.754123595462808
24	21.397164444681525	23.812835059991695	29.617493417869106	25.172507077457677
25	18.6696284668112	26.57085268600473	27.33022674536012	27.429292101823943
26	17.614201399869692	27.232113940400758	27.950528285064376	27.203156374665177
27	20.61912037583872	26.177067894061036	29.21265902847367	23.99115270162658
28	16.542580957352364	24.734523895707042	31.98953717427501	26.733357972665583
29	18.13200839769406	22.351049521247614	28.84345006534503	30.67349201571329
30	19.633229568722783	23.326652772115388	31.68910242976838	25.351015229393454
31	20.347262176465883	24.95570635504262	29.172842375587248	25.524189092904255
32	22.101862047680918	23.72234266706801	25.85548650615539	28.320308779095686
33	20.268962442799282	22.22778935657051	30.27875467226513	27.22449352836508
34	18.080570616453233	20.32097175494279	33.4438928112843	28.15456481731967
35	20.26000845865736	19.47358193657531	29.28638651491886	30.98002308984847
36	21.081869896705317	21.204177509877958	32.03068739926768	25.683265194149048
37	19.78582831973725	21.646351918248218	34.74850735179251	23.81931241022202
38	19.857079172270844	24.60211923658712	28.729334395110744	26.81146719603129
39	20.372790556785407	20.794580362960225	31.912952033316444	26.919677046937927
40	22.918579707604792	19.91385124193665	32.49115079652356	24.67641825393499
41	19.077320510720014	21.307243582660515	28.667609057621746	30.94782684899772
42	20.789627095137035	20.363074531439914	29.99127462821915	28.856023745203903
43	22.441351403870407	19.956906569938237	30.98707197098147	26.61467005520988
44	19.329175128499198	21.02890803305735	30.955066240431623	28.686850598011837
45	20.109695831253596	19.633610589324565	30.448499350359874	29.808194229061964
46	23.02698006881232	20.737617782993524	31.668527317272044	24.56687483092211
47	18.79422220359455	19.53149706804647	35.06113475555623	26.613145972802748
48	19.611701904721986	19.226490076318427	32.038117301002465	29.123690717957118
49	20.716852160196304	17.534187073495065	34.798040030024424	26.95092073628421
50	19.983196991461327	17.910063897154917	33.546577863465075	28.560161247918675
51	19.201914247503364	17.95750096207702	29.197799225004097	33.64278556541552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	686.0
1	789.0
2	892.0
3	1097.0
4	1302.0
5	1011.5
6	721.0
7	729.0
8	737.0
9	795.5
10	854.0
11	884.0
12	914.0
13	1014.0
14	1114.0
15	1107.5
16	1101.0
17	1122.5
18	1144.0
19	1181.5
20	1219.0
21	1316.0
22	1413.0
23	1894.5
24	2376.0
25	2191.0
26	2567.0
27	3128.0
28	3945.0
29	4762.0
30	5273.0
31	5784.0
32	6664.0
33	7544.0
34	8962.0
35	10380.0
36	10652.5
37	10925.0
38	11845.0
39	12765.0
40	15372.0
41	17979.0
42	22161.0
43	26343.0
44	32630.5
45	38918.0
46	47880.0
47	56842.0
48	65802.0
49	74762.0
50	72165.0
51	69568.0
52	55137.5
53	40707.0
54	34630.0
55	28553.0
56	25216.0
57	21879.0
58	20322.0
59	18765.0
60	16552.0
61	14339.0
62	14024.0
63	13709.0
64	11966.0
65	10223.0
66	8739.0
67	7255.0
68	5781.5
69	4308.0
70	3697.0
71	3086.0
72	2794.0
73	2502.0
74	2031.0
75	1205.0
76	850.0
77	586.5
78	323.0
79	312.0
80	301.0
81	228.5
82	156.0
83	155.5
84	155.0
85	93.5
86	32.0
87	21.5
88	11.0
89	9.0
90	7.0
91	3.5
92	0.0
93	2.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	524906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.823394161727173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.07832033494981	20.30326581217534
2	12.801036862940013	7.635407360845191
3	5.550459621109356	4.966006351772855
4	2.9696542147202396	3.542606726785444
5	1.8079212052237792	2.6959173358366804
6	1.1181398962402607	2.0008036112115613
7	0.8538884624637267	1.782609653034483
8	0.6222307226622079	1.4845625681193098
9	0.49019253014323183	1.3157284537436338
>10	5.077167314373466	35.69708579821409
>50	0.539746050454227	10.062578322780045
>100	0.08160446239010337	4.557000809668814
>500	0.006425548219693178	1.2109204422328528
>1k	0.003212774109846589	2.745506753579693
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	4467	0.8510095140844266	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	4056	0.7727097804178272	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	2786	0.5307616982850263	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1711	0.32596312482615936	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1307	0.24899696326580378	TruSeq Adapter, Index 27 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	825	0.1571709982358746	TruSeq Adapter, Index 27 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	756	0.14402578747432873	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	664	0.12649883979226756	TruSeq Adapter, Index 27 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	653	0.12440322648245591	TruSeq Adapter, Index 27 (95% over 22bp)
AGGCAAGGTGTAAATTTTTTTTATTATTTTAAAGATATGATTTATCCTGAG	619	0.11792587625212894	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	597	0.11373464963250564	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	576	0.10973393331377428	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	553	0.10535219639325898	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	543	0.10344709338433929	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.10154199037541961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8102060178393847E-4	0.0	0.0	0.5806753971187222	0.0
2	3.8102060178393847E-4	0.0	0.0	3.003585403862787	0.0
3	3.8102060178393847E-4	0.0	0.0	4.080159114203305	0.0
4	3.8102060178393847E-4	0.0	0.0	5.2651331857513535	0.0
5	3.8102060178393847E-4	0.0	0.0	8.353114652909284	0.0
6	3.8102060178393847E-4	0.0	0.0	10.01684111059885	0.0
7	3.8102060178393847E-4	0.0	0.0	11.76782128609694	0.0
8	3.8102060178393847E-4	0.0	0.0	14.567370157704428	0.0
9	3.8102060178393847E-4	0.0	0.0	15.5738360773167	0.0
10	3.8102060178393847E-4	0.0	0.0	17.80071098444293	0.0
11	3.8102060178393847E-4	0.0	0.0	20.622168540652993	0.0
12	3.8102060178393847E-4	0.0	0.0	22.81494210391956	0.0
13	3.8102060178393847E-4	0.0	0.0	23.704625209085055	0.0
14	3.8102060178393847E-4	0.0	0.0	24.05135395670844	0.0
15	3.8102060178393847E-4	0.0	0.0	24.70366122696254	0.0
16	3.8102060178393847E-4	0.0	0.0	26.061428141419608	0.0
17	3.8102060178393847E-4	0.0	0.0	27.6580949731952	0.0
18	3.8102060178393847E-4	0.0	0.0	29.699984378155328	0.0
19	3.8102060178393847E-4	0.0	0.0	30.614814843038562	0.0
20	3.8102060178393847E-4	0.0	0.0	31.511165808735278	0.0
21	3.8102060178393847E-4	0.0	0.0	32.60069421953645	0.0
22	3.8102060178393847E-4	0.0	0.0	33.62830678254773	0.0
23	3.8102060178393847E-4	0.0	0.0	34.74736428998716	0.0
24	3.8102060178393847E-4	0.0	0.0	35.5435068374147	0.0
25	3.8102060178393847E-4	0.0	0.0	36.281924763671974	0.0
26	3.8102060178393847E-4	0.0	0.0	36.93918530174926	0.0
27	3.8102060178393847E-4	0.0	0.0	37.536816115647376	0.0
28	3.8102060178393847E-4	0.0	0.0	38.16931031460871	0.0
29	3.8102060178393847E-4	0.0	0.0	38.87762761332505	0.0
30	3.8102060178393847E-4	0.0	0.0	39.729970699515725	0.0
31	3.8102060178393847E-4	0.0	0.0	40.41771288573573	0.0
32	3.8102060178393847E-4	0.0	0.0	41.05916106883899	0.0
33	3.8102060178393847E-4	0.0	0.0	41.67069913470221	0.0
34	5.715309026759077E-4	0.0	0.0	42.274807298830645	0.0
35	5.715309026759077E-4	0.0	0.0	42.953404990607844	0.0
36	5.715309026759077E-4	0.0	0.0	43.55351243841754	0.0
37	5.715309026759077E-4	0.0	0.0	44.15933519525401	0.0
38	5.715309026759077E-4	0.0	0.0	44.6885728111319	0.0
39	5.715309026759077E-4	0.0	0.0	45.229241045063304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.0297794E-4	45.000004	1
CTCGTGC	30	2.1632386E-6	45.000004	45
CAACGAT	20	7.0297794E-4	45.000004	3
CCGATCA	20	7.0297794E-4	45.000004	12
CGTGCCT	20	7.0297794E-4	45.000004	23
GTGATAA	20	7.0297794E-4	45.000004	15
ATTCGCA	40	6.8030204E-9	45.000004	39
AGTGCTC	20	7.0297794E-4	45.000004	12
ACGATAT	20	7.0297794E-4	45.000004	39
CCGTAAA	20	7.0297794E-4	45.000004	1
TACGATA	20	7.0297794E-4	45.000004	38
CTACGAT	20	7.0297794E-4	45.000004	37
TCTCGGA	20	7.0297794E-4	45.000004	13
AGTCTAT	20	7.0297794E-4	45.000004	21
ATCCACT	20	7.0297794E-4	45.000004	21
ATCCACG	20	7.0297794E-4	45.000004	36
CATACAG	20	7.0297794E-4	45.000004	44
TATAGTG	30	2.1632386E-6	45.000004	1
TGTAGCG	45	3.8380676E-10	45.000004	1
CGTTTTA	20	7.0297794E-4	45.000004	6
>>END_MODULE