SRR3549913_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814837
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3411	0.4186113296278888	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3114	0.38216232203495915	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	2063	0.2531794702498782	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1214	0.14898685258524097	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	964	0.11830586976290963	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	929	0.11401053216778326	TruSeq Adapter, Index 21 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.032075E-4	45.000004	28
CGTTCCA	20	7.032075E-4	45.000004	39
ACTCGAC	20	7.032075E-4	45.000004	45
TTACGTC	20	7.032075E-4	45.000004	30
CAATCGC	20	7.032075E-4	45.000004	33
CCACGAA	20	7.032075E-4	45.000004	43
CGACCGA	25	3.8898026E-5	45.0	14
CGTTAGT	35	1.2113378E-7	45.0	44
TGATTCG	45	3.8380676E-10	45.0	15
TGCGTCA	35	1.2113378E-7	45.0	12
GATTCGC	30	2.164721E-6	44.999996	10
GCGTAAG	30	2.164721E-6	44.999996	1
CGCATGG	95	0.0	42.63158	2
GCCGACC	55	6.002665E-11	40.909092	14
CATACGA	215	0.0	40.813953	18
CGACGGT	100	0.0	40.5	28
ATAGTAG	50	1.0804797E-9	40.5	1
CGTTCGG	195	0.0	40.384617	45
GATCTAT	45	1.9270374E-8	40.0	9
GGATTAC	45	1.9270374E-8	40.0	9