##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814837
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.510332741394905	33.0	31.0	34.0	31.0	34.0
2	32.696133091649	34.0	31.0	34.0	31.0	34.0
3	32.69970558528884	34.0	31.0	34.0	31.0	34.0
4	36.12266993275956	37.0	35.0	37.0	35.0	37.0
5	36.131843546623436	37.0	35.0	37.0	35.0	37.0
6	36.13454224587249	37.0	36.0	37.0	35.0	37.0
7	36.25026600412107	37.0	37.0	37.0	35.0	37.0
8	36.24903631032955	37.0	37.0	37.0	35.0	37.0
9	37.635723463711145	39.0	38.0	39.0	35.0	39.0
10	37.64773936382368	39.0	37.0	39.0	35.0	39.0
11	37.609826259730475	39.0	37.0	39.0	35.0	39.0
12	37.48329175037461	39.0	37.0	39.0	35.0	39.0
13	37.41179646972339	39.0	37.0	39.0	35.0	39.0
14	38.638101853499535	40.0	38.0	41.0	35.0	41.0
15	38.684730811192914	40.0	38.0	41.0	35.0	41.0
16	38.709316096348104	40.0	38.0	41.0	35.0	41.0
17	38.65573973690444	40.0	38.0	41.0	35.0	41.0
18	38.608184213529825	40.0	38.0	41.0	35.0	41.0
19	38.58967253573414	40.0	38.0	41.0	35.0	41.0
20	38.569733578617566	40.0	38.0	41.0	35.0	41.0
21	38.52915613797606	40.0	38.0	41.0	35.0	41.0
22	38.49594704216917	40.0	38.0	41.0	35.0	41.0
23	38.488215434497945	40.0	38.0	41.0	35.0	41.0
24	38.468877824644686	40.0	37.0	41.0	35.0	41.0
25	38.38305452501543	40.0	37.0	41.0	34.0	41.0
26	38.32129125211545	40.0	37.0	41.0	34.0	41.0
27	38.23085353266973	40.0	37.0	41.0	34.0	41.0
28	38.16343759549456	40.0	37.0	41.0	34.0	41.0
29	38.109948370042105	40.0	37.0	41.0	34.0	41.0
30	37.99914706867754	40.0	37.0	41.0	34.0	41.0
31	37.88552679861126	40.0	36.0	41.0	34.0	41.0
32	37.79828112861836	40.0	36.0	41.0	34.0	41.0
33	37.695569788804384	40.0	36.0	41.0	33.0	41.0
34	37.57762595463878	40.0	36.0	41.0	33.0	41.0
35	37.516395303600596	40.0	36.0	41.0	33.0	41.0
36	37.41877087073856	40.0	35.0	41.0	33.0	41.0
37	37.321485155926894	40.0	35.0	41.0	33.0	41.0
38	37.19438366200848	40.0	35.0	41.0	32.0	41.0
39	37.1337249044901	39.0	35.0	41.0	32.0	41.0
40	36.98374521530073	39.0	35.0	41.0	32.0	41.0
41	36.90726734303916	39.0	35.0	41.0	32.0	41.0
42	36.805794287691896	39.0	35.0	41.0	31.0	41.0
43	36.78358248336784	39.0	35.0	41.0	31.0	41.0
44	36.70197597801769	39.0	35.0	41.0	31.0	41.0
45	36.56712692231698	39.0	35.0	41.0	31.0	41.0
46	36.413071571369485	39.0	35.0	41.0	31.0	41.0
47	36.33259535342651	38.0	35.0	40.0	31.0	41.0
48	36.23081426101171	38.0	35.0	40.0	30.0	41.0
49	36.1707237152952	38.0	35.0	40.0	30.0	41.0
50	36.04205503677423	38.0	35.0	40.0	30.0	41.0
51	34.374553438295024	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	43.0
10	39.0
11	25.0
12	19.0
13	18.0
14	30.0
15	31.0
16	63.0
17	115.0
18	180.0
19	335.0
20	520.0
21	894.0
22	1329.0
23	2049.0
24	2811.0
25	3737.0
26	4849.0
27	5851.0
28	6657.0
29	8014.0
30	9789.0
31	12510.0
32	16134.0
33	23108.0
34	46907.0
35	65648.0
36	54529.0
37	86180.0
38	166084.0
39	296198.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5897449428536	21.78533866282459	24.5559541356124	11.06896225870941
2	31.521764475594505	24.60872542606681	25.598739379777797	18.27077071856089
3	32.08384008089962	24.936398322609307	26.041895495663546	16.937866100827527
4	29.449570895774247	25.772271018620902	25.051881541952564	19.72627654365229
5	24.130961161557465	30.434184996508506	24.6720509746121	20.76280286732193
6	26.207818250766717	34.742163156557694	23.798379307763394	15.251639284912196
7	80.14375881311231	6.602424779434414	8.034858505443419	5.2189579020098495
8	80.42835560977227	5.525522282370584	8.25109807237521	5.795024035481943
9	73.44462757582191	7.832364018816033	10.888312631851523	7.83469577351053
10	38.8946500956633	26.185850667065928	18.457556541983244	16.461942695287522
11	30.715468247023637	25.236581058542995	23.284779655317568	20.7631710391158
12	26.7379856339366	21.871613586520986	29.983665444745387	21.40673533479702
13	24.272461854334058	23.936075558670016	31.623625338564647	20.16783724843128
14	20.28651128998806	28.19741862482926	29.010832841414906	22.505237243767773
15	20.251044073845446	24.625661328584737	34.315574771395994	20.807719826173823
16	21.720049531378667	24.519873299813337	30.489656213451276	23.270420955356716
17	21.773311717558236	24.16213304010495	27.241644647947993	26.822910594388816
18	23.211881640131708	24.541963607445417	29.409317446311352	22.83683730611153
19	23.427262139544474	25.67654635221523	26.80450200469542	24.09168950354488
20	24.83367839212014	25.31230172414851	27.315401730652876	22.538618153078467
21	23.552072377665716	26.73369029634148	28.37524560126749	21.33899172472531
22	22.160505720776058	24.55975857748237	26.505055612349466	26.77468008939211
23	22.226163024015847	25.291438655829324	27.319206172522847	25.163192147631978
24	22.84923242317175	24.390399552303098	28.151151702733184	24.609216321791965
25	22.237699073557042	25.83117850563978	26.695891325504366	25.235231095298815
26	20.569021779816087	26.443080027048353	27.259685065847528	25.728213127288036
27	21.93432551540983	25.770798331445434	28.529386858966888	23.765489294177854
28	20.208458869688048	25.155337816029466	29.621261675648014	25.014941638634475
29	21.372740805829878	23.87360907764375	28.383836276457746	26.36981384006863
30	23.503350976943853	24.073771809576638	28.57896732720777	23.84390988627173
31	24.620384199539295	25.716922525609416	26.096016749362143	23.566676525489147
32	23.368845548250754	26.024591421351754	26.58237168906174	24.02419134133575
33	23.792856730855373	24.536072858743527	26.72117245534997	24.949897955051135
34	22.586480486281303	23.46825193259511	29.0799264147308	24.86534116639279
35	21.884990495031523	23.990442260231188	27.971361143394326	26.153206101342967
36	23.736649170324863	25.42925763066724	26.99570588964419	23.83838730936371
37	22.34716882026712	26.156396923556493	29.604693944923955	21.891740311252434
38	21.766684625268613	27.632029473379337	27.10983914574326	23.49144675560879
39	23.91938510401467	23.824028609402863	27.072653794562594	25.183932492019878
40	24.626029500378603	22.88997676835981	28.692732411512978	23.791261319748614
41	20.372172594028008	25.140365496412166	26.65465608459115	27.832805824968677
42	20.75335312461265	24.445748045314584	27.967065805799194	26.833833024273567
43	22.115466037992874	22.283843271721828	29.41484002321937	26.185850667065928
44	22.220763171039117	22.421539522628454	29.02555971316963	26.332137593162802
45	23.089157708842382	22.206159023215687	28.060581441441663	26.644101826500272
46	24.04628164896783	23.786843258222195	28.188582501776427	23.978292591033544
47	21.367218228921857	23.545445285376093	30.511010177495624	24.576326308206426
48	20.277920614797807	23.90797177840476	29.710113801901482	26.103993804895946
49	22.346923372404543	21.79478840553387	30.932566881474454	24.92572134058714
50	21.55400405234421	21.484296859371874	30.882740965370985	26.078958122912926
51	21.56492648222896	21.827678419119405	27.631047681929022	28.976347416722607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	460.0
1	854.5
2	1249.0
3	1707.0
4	2165.0
5	1671.0
6	1177.0
7	1092.0
8	1007.0
9	1024.5
10	1042.0
11	1140.0
12	1238.0
13	1249.0
14	1260.0
15	1406.0
16	1552.0
17	1601.0
18	1650.0
19	1523.5
20	1397.0
21	1620.0
22	1843.0
23	1976.5
24	2110.0
25	2900.0
26	4912.5
27	6135.0
28	7318.0
29	8501.0
30	9545.5
31	10590.0
32	12545.0
33	14500.0
34	14712.5
35	14925.0
36	17384.0
37	19843.0
38	21955.0
39	24067.0
40	28252.5
41	32438.0
42	36814.0
43	41190.0
44	46792.5
45	52395.0
46	62363.0
47	72331.0
48	82723.5
49	93116.0
50	90232.0
51	87348.0
52	75207.5
53	63067.0
54	55263.5
55	47460.0
56	44059.5
57	40659.0
58	37235.5
59	33812.0
60	34338.5
61	34865.0
62	31637.5
63	28410.0
64	24990.0
65	21570.0
66	18380.5
67	15191.0
68	13444.5
69	11698.0
70	9374.5
71	7051.0
72	5911.5
73	4772.0
74	3926.0
75	2518.5
76	1957.0
77	1452.0
78	947.0
79	734.0
80	521.0
81	408.0
82	295.0
83	213.0
84	131.0
85	89.5
86	48.0
87	40.5
88	33.0
89	25.5
90	18.0
91	17.0
92	16.0
93	11.0
94	6.0
95	7.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	814837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.617412668992298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05175181755342	19.34648138131204
2	11.904307535514185	6.5753234749377985
3	4.772957221641865	3.9545018772459093
4	2.427372132933655	2.681509515857631
5	1.4860657866463396	2.052064604154131
6	0.9509558970803137	1.5757764863807275
7	0.6740454110893277	1.3030773192986165
8	0.5324955923790332	1.1764920415321016
9	0.45603249026191794	1.133499372662844
>10	5.877422336939306	40.9436496679951
>50	0.7835935500410531	13.197330184524503
>100	0.07719913672088179	4.106827447387141
>500	0.0040161400606239074	0.7454747097960873
>1k	0.0017849511380550703	1.207991916915399
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3411	0.4186113296278888	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	3114	0.38216232203495915	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	2063	0.2531794702498782	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	1214	0.14898685258524097	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	964	0.11830586976290963	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	929	0.11401053216778326	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2272393128932534E-4	0.0	0.0	0.2798105633396618	0.0
2	1.2272393128932534E-4	0.0	0.0	1.4664282549761487	0.0
3	1.2272393128932534E-4	0.0	0.0	2.0400399098224553	0.0
4	1.2272393128932534E-4	0.0	0.0	2.6513278115745846	0.0
5	1.2272393128932534E-4	0.0	0.0	4.435488324658797	0.0
6	1.2272393128932534E-4	0.0	0.0	5.4073391365389645	0.0
7	1.2272393128932534E-4	0.0	0.0	6.365567592046016	0.0
8	1.2272393128932534E-4	0.0	0.0	7.937906599724853	0.0
9	1.2272393128932534E-4	0.0	0.0	8.501209444342857	0.0
10	1.2272393128932534E-4	0.0	0.0	9.763915973378724	0.0
11	1.2272393128932534E-4	0.0	0.0	11.455910814064653	0.0
12	1.2272393128932534E-4	0.0	0.0	12.801701444583395	0.0
13	1.2272393128932534E-4	0.0	0.0	13.351872828553441	0.0
14	1.2272393128932534E-4	0.0	0.0	13.55596272628759	0.0
15	1.2272393128932534E-4	0.0	0.0	14.018018327591898	0.0
16	1.2272393128932534E-4	0.0	0.0	14.957347297680395	0.0
17	1.2272393128932534E-4	0.0	0.0	16.052412936575045	0.0
18	1.2272393128932534E-4	0.0	0.0	17.36163183556957	0.0
19	2.454478625786507E-4	0.0	0.0	18.00715971415142	0.0
20	2.454478625786507E-4	0.0	0.0	18.691836526814566	0.0
21	2.454478625786507E-4	0.0	0.0	19.46573363752505	0.0
22	2.454478625786507E-4	0.0	0.0	20.251166797776733	0.0
23	2.454478625786507E-4	0.0	0.0	21.018682264060175	0.0
24	2.454478625786507E-4	0.0	0.0	21.676850707564824	0.0
25	2.454478625786507E-4	0.0	0.0	22.259421209395253	0.0
26	2.454478625786507E-4	0.0	0.0	22.80762901046467	0.0
27	2.454478625786507E-4	0.0	0.0	23.328837546650433	0.0
28	2.454478625786507E-4	0.0	0.0	23.86219575203384	0.0
29	2.454478625786507E-4	0.0	0.0	24.42819852314021	0.0
30	2.454478625786507E-4	0.0	0.0	25.111402648627884	0.0
31	2.454478625786507E-4	0.0	0.0	25.69102777610737	0.0
32	2.454478625786507E-4	0.0	0.0	26.262307676259177	0.0
33	2.454478625786507E-4	0.0	0.0	26.80904279015312	0.0
34	2.454478625786507E-4	0.0	0.0	27.340928308361057	0.0
35	2.454478625786507E-4	0.0	0.0	27.916626270039284	0.0
36	2.454478625786507E-4	0.0	0.0	28.47219750698606	0.0
37	2.454478625786507E-4	0.0	0.0	29.020528031986764	0.0
38	2.454478625786507E-4	0.0	0.0	29.530936862219068	0.0
39	3.6817179386797606E-4	0.0	0.0	30.084421792333927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.032075E-4	45.000004	28
CGTTCCA	20	7.032075E-4	45.000004	39
ACTCGAC	20	7.032075E-4	45.000004	45
TTACGTC	20	7.032075E-4	45.000004	30
CAATCGC	20	7.032075E-4	45.000004	33
CCACGAA	20	7.032075E-4	45.000004	43
CGACCGA	25	3.8898026E-5	45.0	14
CGTTAGT	35	1.2113378E-7	45.0	44
TGATTCG	45	3.8380676E-10	45.0	15
TGCGTCA	35	1.2113378E-7	45.0	12
GATTCGC	30	2.164721E-6	44.999996	10
GCGTAAG	30	2.164721E-6	44.999996	1
CGCATGG	95	0.0	42.63158	2
GCCGACC	55	6.002665E-11	40.909092	14
CATACGA	215	0.0	40.813953	18
CGACGGT	100	0.0	40.5	28
ATAGTAG	50	1.0804797E-9	40.5	1
CGTTCGG	195	0.0	40.384617	45
GATCTAT	45	1.9270374E-8	40.0	9
GGATTAC	45	1.9270374E-8	40.0	9
>>END_MODULE