FastQCFastQC Report
Sat 18 Jun 2016
SRR3549912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725484
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC31680.4366739997022678No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC26960.3716139845951117No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC18260.2516940414950571No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG17110.23584255476344068No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT16560.2282614089352763No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC15660.21585589758009827No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC11050.15231211163857508No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.11688748476878882No Hit
ACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC8130.11206311924177514No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT207.0315634E-445.00000423
CGTCTCA207.0315634E-445.00000415
CGATTGT207.0315634E-445.00000410
ATCGTGT207.0315634E-445.00000428
CAAGGCG302.16439E-645.0000041
TCTCGAG207.0315634E-445.0000041
TCGTCTC207.0315634E-445.00000414
TCGTAAG207.0315634E-445.0000041
CGGTCTA406.8084773E-945.00000431
CCAACGG207.0315634E-445.0000042
TAGTGCG406.8084773E-945.0000041
CATCCGC207.0315634E-445.00000417
CGTGCAT253.8893777E-545.021
TACGATG253.8893777E-545.01
GCGATAC253.8893777E-545.09
ACGTAAG253.8893777E-545.01
ATCGGTA253.8893777E-545.012
CACGTGC253.8893777E-545.019
TTCGCTA253.8893777E-545.027
CGAATAT700.041.78571714