##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	725484
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52889243594621	33.0	31.0	34.0	31.0	34.0
2	32.703050653081256	34.0	31.0	34.0	31.0	34.0
3	32.71271868159739	34.0	31.0	34.0	31.0	34.0
4	36.137483390398685	37.0	35.0	37.0	35.0	37.0
5	36.147977074615014	37.0	35.0	37.0	35.0	37.0
6	36.1418363464942	37.0	36.0	37.0	35.0	37.0
7	36.25097314344631	37.0	37.0	37.0	35.0	37.0
8	36.22887754933258	37.0	37.0	37.0	35.0	37.0
9	37.612923234695735	39.0	38.0	39.0	35.0	39.0
10	37.64092109543422	39.0	37.0	39.0	35.0	39.0
11	37.62534390834257	39.0	37.0	39.0	35.0	39.0
12	37.50857220834643	39.0	37.0	39.0	35.0	39.0
13	37.43801379492863	39.0	37.0	39.0	35.0	39.0
14	38.675259550865356	40.0	38.0	41.0	35.0	41.0
15	38.69902299706127	40.0	38.0	41.0	35.0	41.0
16	38.722145491837175	40.0	38.0	41.0	35.0	41.0
17	38.67550628270231	40.0	38.0	41.0	35.0	41.0
18	38.609001714717344	40.0	38.0	41.0	35.0	41.0
19	38.57852550848813	40.0	38.0	41.0	35.0	41.0
20	38.55841617458138	40.0	38.0	41.0	35.0	41.0
21	38.55242293420668	40.0	38.0	41.0	35.0	41.0
22	38.52930043943078	40.0	38.0	41.0	35.0	41.0
23	38.495335527730454	40.0	38.0	41.0	35.0	41.0
24	38.48016772251352	40.0	38.0	41.0	35.0	41.0
25	38.416730072613596	40.0	37.0	41.0	34.0	41.0
26	38.343293580561394	40.0	37.0	41.0	34.0	41.0
27	38.26104090510611	40.0	37.0	41.0	34.0	41.0
28	38.181153547149215	40.0	37.0	41.0	34.0	41.0
29	38.11240495999912	40.0	37.0	41.0	34.0	41.0
30	38.005548020356066	40.0	37.0	41.0	34.0	41.0
31	37.906309443075244	40.0	36.0	41.0	34.0	41.0
32	37.809433426512506	40.0	36.0	41.0	33.0	41.0
33	37.69463971638244	40.0	36.0	41.0	33.0	41.0
34	37.572912703794984	40.0	36.0	41.0	33.0	41.0
35	37.52529345926306	40.0	36.0	41.0	33.0	41.0
36	37.416257284791946	40.0	36.0	41.0	33.0	41.0
37	37.308682203880444	40.0	35.0	41.0	33.0	41.0
38	37.19204282933876	40.0	35.0	41.0	32.0	41.0
39	37.0832451163637	39.0	35.0	41.0	32.0	41.0
40	36.95212161811977	39.0	35.0	41.0	31.0	41.0
41	36.88328205721973	39.0	35.0	41.0	31.0	41.0
42	36.82446614949468	39.0	35.0	41.0	31.0	41.0
43	36.78889541326893	39.0	35.0	41.0	31.0	41.0
44	36.686093697448875	39.0	35.0	41.0	31.0	41.0
45	36.5549963886178	39.0	35.0	41.0	31.0	41.0
46	36.41895203753632	39.0	35.0	41.0	31.0	41.0
47	36.340591935866264	39.0	35.0	40.0	30.0	41.0
48	36.241977769323654	38.0	35.0	40.0	30.0	41.0
49	36.17197209035623	38.0	35.0	40.0	30.0	41.0
50	36.06329291893412	38.0	35.0	40.0	30.0	41.0
51	34.266689547943166	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	22.0
10	28.0
11	14.0
12	13.0
13	15.0
14	18.0
15	28.0
16	40.0
17	97.0
18	165.0
19	287.0
20	520.0
21	771.0
22	1187.0
23	1820.0
24	2519.0
25	3488.0
26	4498.0
27	5083.0
28	6030.0
29	7216.0
30	8718.0
31	11400.0
32	14660.0
33	20680.0
34	41422.0
35	56627.0
36	48020.0
37	75432.0
38	149514.0
39	265033.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17159303306482	20.66868462984711	25.087941291606707	11.071781045481362
2	31.137419984451757	25.74184957903965	24.715775950951365	18.40495448555723
3	31.901048127870496	25.34321914749326	25.784993190752658	16.970739533883584
4	29.121937906280497	24.925153414823757	26.17094243291375	19.781966245981994
5	25.47499324588826	30.00093730530239	23.750627167518513	20.773442281290837
6	25.85694515661269	35.23013601954006	24.03154308020577	14.881375743641486
7	79.84131972586577	6.199034024182477	8.751950422063064	5.207695827888692
8	79.70044825247696	6.234320812037206	8.33677930870977	5.728451626776056
9	72.79926228559141	8.077228443356436	10.755302666909264	8.36820660414289
10	37.87002883592195	27.651471293646722	18.087511233879727	16.390988636551597
11	31.21943419841099	24.622872454802586	23.402721493513294	20.754971853273123
12	27.570008435747724	22.763975497736684	29.59803386428922	20.067982202226375
13	25.03004890528254	24.722392223674127	30.54154192235804	19.706016948685292
14	20.198240071455746	29.14123536838855	28.530746370698733	22.12977818945697
15	19.580583444982935	25.5836103897536	34.56575196696274	20.27005419830072
16	21.621703579954897	25.13797685407259	30.489025257621122	22.75129430835139
17	21.62225493601513	24.347470102717633	27.99827425553148	26.032000705735758
18	22.083188602367525	25.23198306234183	29.873160538344056	22.81166779694659
19	23.83774142503487	26.48342348004918	27.021960511878966	22.65687458303698
20	24.893726119390642	26.55330786068335	27.043463398227942	21.509502621698065
21	23.86089837956454	27.028714623616786	28.388910024204534	20.721476972614145
22	21.453539981584708	25.64550010751443	26.530288745168747	26.37067116573212
23	21.917230428238252	26.50423717132287	27.14794537164155	24.430587028797326
24	22.40145888813537	25.30131608691577	27.820324087092203	24.47690093785666
25	21.265389726031174	27.283716801473222	26.73332561434849	24.717567858147113
26	20.446074620529192	26.75124468630597	27.86787854728705	24.934802145877786
27	22.24942245452691	26.00884375120609	27.014517205065857	24.72721658920114
28	19.193255812671264	25.37616267209201	29.82767366337507	25.602907851861655
29	20.623611271923295	24.40908414244835	29.218838733865944	25.748465851762408
30	22.73806176290587	24.78249003423921	27.298879093129553	25.18056910972537
31	23.341934487872923	26.128350177260973	24.559328668861063	25.97038666600504
32	22.151970270881232	25.809252857402782	27.02182267286391	25.016954198852076
33	22.98162330251253	24.87029348683086	26.2038859575125	25.944197253144107
34	20.358684684982713	25.46686074399987	29.10898103886509	25.06547353215233
35	20.65076555788963	23.55503360515187	28.377055868909583	27.417144968048916
36	22.489400179742074	25.09621163251016	27.8943436381781	24.52004454956967
37	20.577848718924194	25.991062518263668	29.087478152516113	24.343610610296025
38	20.604727326860413	26.343930396810954	26.990671055460908	26.060671220867725
39	23.112570366817188	24.583312657481073	28.928687607169834	23.375429368531904
40	23.03744810361083	24.07702995517475	29.62077730177371	23.264744639440703
41	19.33550567621064	25.42964420993433	27.650919937586494	27.583930176268534
42	20.276532632008426	23.549795722579685	29.56274707643449	26.610924568977403
43	22.32867988818499	22.07271283722315	29.070661792679093	26.527945481912763
44	21.022655220514856	23.36660767156822	29.00711800673757	26.603619101179348
45	21.693104189754703	23.024904753240598	27.578416615666228	27.703574441338475
46	22.812494831036936	23.786878828478645	28.227087020526987	25.173539319957435
47	19.957297473135178	23.793357262186348	31.22467208098318	25.0246731836953
48	19.75880929145233	23.455238158250218	29.652204597206833	27.133747953090626
49	20.833402252840862	21.436861460762746	32.15549894966671	25.574237336729688
50	20.65076555788963	21.531419025092212	30.901439590673263	26.916375826344897
51	20.43780427962574	21.711436778757353	27.800888785969093	30.049870155647817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	505.0
1	827.5
2	1150.0
3	1641.5
4	2133.0
5	1637.0
6	1141.0
7	1093.0
8	1045.0
9	1035.0
10	1025.0
11	1031.0
12	1037.0
13	1147.0
14	1257.0
15	1282.0
16	1307.0
17	1339.0
18	1371.0
19	1689.5
20	2008.0
21	1886.0
22	1764.0
23	2338.0
24	2912.0
25	3435.0
26	4505.0
27	5052.0
28	6957.0
29	8862.0
30	9434.5
31	10007.0
32	10820.5
33	11634.0
34	12983.5
35	14333.0
36	16551.0
37	18769.0
38	20914.5
39	23060.0
40	25083.0
41	27106.0
42	32897.5
43	38689.0
44	43765.0
45	48841.0
46	58621.5
47	68402.0
48	74343.5
49	80285.0
50	78242.5
51	76200.0
52	66134.5
53	56069.0
54	49240.5
55	42412.0
56	38517.5
57	34623.0
58	32740.0
59	30857.0
60	29670.5
61	28484.0
62	26365.0
63	24246.0
64	21060.5
65	17875.0
66	15110.0
67	12345.0
68	10561.0
69	8777.0
70	7504.0
71	6231.0
72	5148.0
73	4065.0
74	3260.0
75	1861.0
76	1267.0
77	1070.5
78	874.0
79	685.5
80	497.0
81	384.5
82	272.0
83	216.5
84	161.0
85	113.0
86	65.0
87	48.0
88	31.0
89	22.5
90	14.0
91	10.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	725484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.105816258593723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.19954329768547	19.730154653619422
2	11.911634596958205	6.6957242664323084
3	4.7515452147293935	4.006381702487538
4	2.426030898724917	2.72742314708934
5	1.4447663281043548	2.0303168477152065
6	0.9241802888237554	1.5584904832496727
7	0.6587688034021633	1.29606644617204
8	0.5195376563675627	1.1681623927429698
9	0.43255901743188274	1.0941681838444504
>10	5.989141117723571	41.91178365397168
>50	0.6554287313204258	11.215805887140007
>100	0.07896731702577943	3.8754212463329862
>500	0.004441911582700093	0.785820794633702
>1k	0.0034548201198778498	1.9042802945686603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	3168	0.4366739997022678	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2696	0.3716139845951117	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	1826	0.2516940414950571	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	1711	0.23584255476344068	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	1656	0.2282614089352763	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	1566	0.21585589758009827	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1105	0.15231211163857508	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	848	0.11688748476878882	No Hit
ACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	813	0.11206311924177514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.13517045172602E-4	0.0	0.0	0.2558292119467831	0.0
2	4.13517045172602E-4	0.0	0.0	1.4599908474894001	0.0
3	4.13517045172602E-4	0.0	0.0	2.0307822088426484	0.0
4	4.13517045172602E-4	0.0	0.0	2.6830364280948995	0.0
5	4.13517045172602E-4	0.0	0.0	4.656891123718786	0.0
6	4.13517045172602E-4	0.0	0.0	5.629069696919574	0.0
7	5.51356060230136E-4	0.0	0.0	6.643289169712909	0.0
8	5.51356060230136E-4	0.0	0.0	8.218789111820522	0.0
9	5.51356060230136E-4	0.0	0.0	8.811772554598035	0.0
10	5.51356060230136E-4	0.0	0.0	10.331585534622404	0.0
11	5.51356060230136E-4	0.0	0.0	12.017770205821217	0.0
12	5.51356060230136E-4	0.0	0.0	13.643443549409774	0.0
13	5.51356060230136E-4	0.0	0.0	14.209961901296237	0.0
14	5.51356060230136E-4	0.0	0.0	14.447596363255427	0.0
15	5.51356060230136E-4	0.0	0.0	14.89736506938816	0.0
16	5.51356060230136E-4	0.0	0.0	15.831086557387895	0.0
17	5.51356060230136E-4	0.0	0.0	16.919876937327356	0.0
18	5.51356060230136E-4	0.0	0.0	18.261326231867276	0.0
19	5.51356060230136E-4	0.0	0.0	18.932602235197468	0.0
20	5.51356060230136E-4	0.0	0.0	19.599053873000646	0.0
21	6.891950752876701E-4	0.0	0.0	20.40816889138837	0.0
22	6.891950752876701E-4	0.0	0.0	21.240716542335875	0.0
23	6.891950752876701E-4	0.0	0.0	22.031498971720946	0.0
24	6.891950752876701E-4	0.0	0.0	22.691472175816422	0.0
25	6.891950752876701E-4	0.0	0.0	23.29562057881359	0.0
26	8.27034090345204E-4	0.0	0.0	23.845322570863036	0.0
27	8.27034090345204E-4	0.0	0.0	24.364286462554652	0.0
28	8.27034090345204E-4	0.0	0.0	24.922534473537667	0.0
29	8.27034090345204E-4	0.0	0.0	25.50917732162253	0.0
30	8.27034090345204E-4	0.0	0.0	26.183623622299045	0.0
31	8.27034090345204E-4	0.0	0.0	26.78763418628116	0.0
32	8.27034090345204E-4	0.0	0.0	27.363525591191536	0.0
33	8.27034090345204E-4	0.0	0.0	27.88869223856074	0.0
34	9.648731054027381E-4	0.0	0.0	28.44542402037812	0.0
35	9.648731054027381E-4	0.0	0.0	29.03110199535758	0.0
36	9.648731054027381E-4	0.0	0.0	29.59183110861163	0.0
37	9.648731054027381E-4	0.0	0.0	30.134365471878084	0.0
38	9.648731054027381E-4	0.0	0.0	30.691510770740635	0.0
39	9.648731054027381E-4	0.0	0.0	31.23183970976617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTT	20	7.0315634E-4	45.000004	23
CGTCTCA	20	7.0315634E-4	45.000004	15
CGATTGT	20	7.0315634E-4	45.000004	10
ATCGTGT	20	7.0315634E-4	45.000004	28
CAAGGCG	30	2.16439E-6	45.000004	1
TCTCGAG	20	7.0315634E-4	45.000004	1
TCGTCTC	20	7.0315634E-4	45.000004	14
TCGTAAG	20	7.0315634E-4	45.000004	1
CGGTCTA	40	6.8084773E-9	45.000004	31
CCAACGG	20	7.0315634E-4	45.000004	2
TAGTGCG	40	6.8084773E-9	45.000004	1
CATCCGC	20	7.0315634E-4	45.000004	17
CGTGCAT	25	3.8893777E-5	45.0	21
TACGATG	25	3.8893777E-5	45.0	1
GCGATAC	25	3.8893777E-5	45.0	9
ACGTAAG	25	3.8893777E-5	45.0	1
ATCGGTA	25	3.8893777E-5	45.0	12
CACGTGC	25	3.8893777E-5	45.0	19
TTCGCTA	25	3.8893777E-5	45.0	27
CGAATAT	70	0.0	41.785717	14
>>END_MODULE