##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549910_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	969437
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55492930432818	34.0	31.0	34.0	31.0	34.0
2	32.73005362906512	34.0	31.0	34.0	31.0	34.0
3	32.74743278830909	34.0	31.0	34.0	31.0	34.0
4	36.15287326561705	37.0	35.0	37.0	35.0	37.0
5	36.16812954322973	37.0	35.0	37.0	35.0	37.0
6	36.16103779822722	37.0	36.0	37.0	35.0	37.0
7	36.25986732505568	37.0	37.0	37.0	35.0	37.0
8	36.23172315477953	37.0	37.0	37.0	35.0	37.0
9	37.61763373999548	39.0	38.0	39.0	35.0	39.0
10	37.6695979212677	39.0	37.0	39.0	35.0	39.0
11	37.63987757842954	39.0	37.0	39.0	35.0	39.0
12	37.52066921316187	39.0	37.0	39.0	35.0	39.0
13	37.454876387016384	39.0	37.0	39.0	35.0	39.0
14	38.72802564787604	40.0	38.0	41.0	35.0	41.0
15	38.7681716295128	40.0	38.0	41.0	35.0	41.0
16	38.788750584101905	40.0	38.0	41.0	35.0	41.0
17	38.72919024134627	40.0	38.0	41.0	35.0	41.0
18	38.68076832223239	40.0	38.0	41.0	35.0	41.0
19	38.65848528578959	40.0	38.0	41.0	35.0	41.0
20	38.627237252137064	40.0	38.0	41.0	35.0	41.0
21	38.60856868471082	40.0	38.0	41.0	35.0	41.0
22	38.56965847187595	40.0	38.0	41.0	35.0	41.0
23	38.53662280271951	40.0	38.0	41.0	35.0	41.0
24	38.50819599416981	40.0	38.0	41.0	35.0	41.0
25	38.423321989979755	40.0	37.0	41.0	35.0	41.0
26	38.33081262629753	40.0	37.0	41.0	34.0	41.0
27	38.253819484917535	40.0	37.0	41.0	34.0	41.0
28	38.15447316328962	40.0	37.0	41.0	34.0	41.0
29	38.081437989265936	40.0	37.0	41.0	34.0	41.0
30	37.97908992538969	40.0	37.0	41.0	34.0	41.0
31	37.885792475426456	40.0	36.0	41.0	34.0	41.0
32	37.78572202216338	40.0	36.0	41.0	33.0	41.0
33	37.67949748152794	40.0	36.0	41.0	33.0	41.0
34	37.587188233995604	40.0	36.0	41.0	33.0	41.0
35	37.517157896799894	40.0	36.0	41.0	33.0	41.0
36	37.40592632631105	40.0	35.0	41.0	33.0	41.0
37	37.29961307439266	40.0	35.0	41.0	33.0	41.0
38	37.1730231051631	40.0	35.0	41.0	32.0	41.0
39	37.061661562329476	39.0	35.0	41.0	32.0	41.0
40	36.93398642717371	39.0	35.0	41.0	31.0	41.0
41	36.844299320120854	39.0	35.0	41.0	31.0	41.0
42	36.76813139997751	39.0	35.0	41.0	31.0	41.0
43	36.669652592174636	39.0	35.0	41.0	31.0	41.0
44	36.57150593591951	39.0	35.0	41.0	31.0	41.0
45	36.470015070602834	39.0	35.0	41.0	31.0	41.0
46	36.3101790008015	38.0	35.0	40.0	30.0	41.0
47	36.246587452304794	38.0	35.0	40.0	30.0	41.0
48	36.13513513513514	38.0	35.0	40.0	30.0	41.0
49	36.05049425594443	38.0	35.0	40.0	30.0	41.0
50	35.944068567632556	38.0	35.0	40.0	30.0	41.0
51	34.21206638492238	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	14.0
9	31.0
10	41.0
11	20.0
12	17.0
13	27.0
14	31.0
15	48.0
16	50.0
17	114.0
18	212.0
19	399.0
20	629.0
21	1086.0
22	1696.0
23	2345.0
24	3379.0
25	4380.0
26	5669.0
27	7068.0
28	8096.0
29	9409.0
30	11984.0
31	14905.0
32	19458.0
33	27613.0
34	55198.0
35	76363.0
36	65912.0
37	105227.0
38	198974.0
39	348846.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83830408783655	21.509185228127254	25.619921665874113	11.032589018162088
2	31.186348365082	26.146928578133494	24.77943383634006	17.887289220444444
3	32.351148140621824	24.63574218850735	26.352408666060818	16.660701004810008
4	29.142069056576137	24.963664477423496	26.755735545476394	19.138530920523973
5	25.213603359475655	31.258967833907718	23.75471536572258	19.772713440894044
6	26.069770392506165	35.828424126580686	23.377279802607077	14.724525678306069
7	80.47578130399397	6.607959052522237	8.21559317418254	4.700666469301254
8	79.83396548718483	6.315727582091461	7.9533791262351246	5.896927804488585
9	73.30914747425568	8.30626435756011	10.67072950588847	7.713858662295745
10	39.07030575478345	26.214699872193858	17.777328490660043	16.937665882362648
11	30.77672917373692	25.08167111426529	23.329004360262708	20.81259535173508
12	26.782039472394803	22.148009617953512	29.478862473786332	21.591088435865352
13	23.785145398824266	24.839881291925106	30.981177735118425	20.393795574132202
14	20.048027875973375	28.16284090662931	28.84457680076168	22.944554416635636
15	19.345145687651698	25.396699321358685	33.35368879050418	21.904466200485437
16	21.753038103559074	23.995061050898613	30.06250019341123	24.18940065213108
17	21.561174166036576	24.764064090807345	27.515351693818165	26.159410049337918
18	22.834490534196654	24.68649329456169	28.768140683716425	23.710875487525236
19	23.053586772528796	26.777088144974865	26.504558831569252	23.664766250927087
20	24.21467305250367	25.801986101211323	27.187532557556604	22.795808288728406
21	23.98237327438503	26.946361651143913	27.045181894233455	22.026083180237602
22	22.858421950059675	24.77582349342969	25.711005459870005	26.65474909664063
23	22.07219241683575	26.20686027044563	26.685282282396894	25.035665030321724
24	21.976982516656573	24.863503249824383	28.360068782190073	24.799445451328967
25	21.73467693104348	26.791323211307184	26.749546386201477	24.72445347144786
26	20.914407021807502	25.959912815376345	27.92053532101622	25.20514484179993
27	23.188716750031205	24.974082895536277	27.160712867365284	24.676487487067238
28	19.67915398318818	26.114848102558497	28.548322376802204	25.65767553745112
29	20.48395099423686	26.041712870459865	28.25103642629691	25.223299709006362
30	21.93623721809669	25.11333897922196	27.316267070474925	25.634156732206424
31	23.37305054376922	26.212327361138477	23.712938540616875	26.70168355447543
32	21.81400132241703	27.28129832057163	25.237947385957003	25.666752971054336
33	20.96670541768057	25.61218521678046	25.900290581027956	27.520818784511007
34	19.706798894616153	24.83792139148805	27.344840355794137	28.11043935810166
35	20.513762111411058	23.876332345474744	27.31348194880121	28.296423594312987
36	21.052528426292785	25.185236379465607	28.258360264772236	25.503874929469372
37	19.913826272362208	26.335801088673115	27.483683828861494	26.266688810103183
38	20.043489159171767	27.78994406031542	25.93938543711453	26.227181343398282
39	21.59449247346656	24.228289202908492	29.11628089293064	25.06093743069431
40	21.663295294072746	23.356752424345263	29.701053291755937	25.278898989826054
41	18.655157581152775	24.301011824388794	27.91022005555802	29.133610538900413
42	20.39988158075254	23.18386857526585	28.629812973921975	27.78643687005963
43	21.503099221506915	22.846972005401074	29.380248536005947	26.269680237086064
44	20.351915596371914	24.128334280618542	28.09888626078848	27.42086386222106
45	21.395717308087065	23.861684668524102	27.150397601907084	27.592200421481746
46	22.602293908732594	23.679723385841474	27.956122986847003	25.76185971857893
47	20.295903704933895	22.94847421750975	30.85079277972679	25.90482929782956
48	19.696483629157953	23.59812963606712	29.343732496283927	27.361654238491003
49	20.647344799094732	23.360259614601052	30.559695988496415	25.4326995978078
50	20.534908405600362	21.935618302169196	30.5265839863756	27.002889305854843
51	20.138389601387196	22.1492474498085	27.336381838118413	30.375981110685895
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	583.0
1	1130.5
2	1678.0
3	2184.5
4	2691.0
5	2051.0
6	1411.0
7	1317.0
8	1223.0
9	1269.0
10	1315.0
11	1333.0
12	1351.0
13	1512.0
14	1673.0
15	1634.0
16	1595.0
17	1682.5
18	1770.0
19	1967.0
20	2164.0
21	2333.0
22	2502.0
23	2827.0
24	3152.0
25	4227.0
26	6286.5
27	7271.0
28	9206.5
29	11142.0
30	12690.0
31	14238.0
32	15685.0
33	17132.0
34	19260.5
35	21389.0
36	22625.5
37	23862.0
38	25493.5
39	27125.0
40	32845.0
41	38565.0
42	43420.5
43	48276.0
44	56694.5
45	65113.0
46	75834.0
47	86555.0
48	96304.5
49	106054.0
50	101210.0
51	96366.0
52	84950.0
53	73534.0
54	65820.0
55	58106.0
56	52247.0
57	46388.0
58	44842.5
59	43297.0
60	40771.5
61	38246.0
62	35170.5
63	32095.0
64	28836.5
65	25578.0
66	22686.0
67	19794.0
68	17230.0
69	14666.0
70	12111.0
71	9556.0
72	8223.5
73	6891.0
74	5585.5
75	3602.5
76	2925.0
77	2087.0
78	1249.0
79	944.0
80	639.0
81	488.5
82	338.0
83	288.5
84	239.0
85	149.5
86	60.0
87	41.0
88	22.0
89	19.0
90	16.0
91	14.5
92	13.0
93	6.5
94	0.0
95	2.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	969437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.60540184298597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.97182805583913	17.916567750580807
2	11.82840137111207	6.057419405349016
3	4.906523738852394	3.769005359563965
4	2.430615848170346	2.4894758207332757
5	1.47641625892453	1.8902115798640307
6	0.9739333988626149	1.4962773627709434
7	0.728142909472194	1.3051074257309538
8	0.5094173779072122	1.043506933369153
9	0.41448084894466153	0.9551653824105024
>10	5.424397376386443	35.52408328593778
>50	1.2235428286724872	19.82518052800976
>100	0.10257002409580056	4.771127270519317
>500	0.004864981380037969	0.8363824202818506
>1k	0.004864981380037969	2.1204894748786596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3905	0.40281111614266835	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3270	0.33730918048310515	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	2226	0.2296178090995083	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTC	1462	0.15080918099886842	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	1455	0.1500871124167945	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1347	0.13894662572193964	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	1306	0.1347173668840781	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1133	0.11687195764139392	No Hit
ACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1119	0.11542782047724608	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1110	0.11449944658600818	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1050	0.10831028731108881	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1044	0.10769137138359687	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	979	0.10098644883576756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0315265458198934E-4	0.0	0.0	0.2537555302716938	0.0
2	1.0315265458198934E-4	0.0	0.0	1.3687325736484166	0.0
3	1.0315265458198934E-4	0.0	0.0	1.8652063001515313	0.0
4	1.0315265458198934E-4	0.0	0.0	2.4980478360120357	0.0
5	1.0315265458198934E-4	0.0	0.0	4.180158174280536	0.0
6	1.0315265458198934E-4	0.0	0.0	5.0812997647087945	0.0
7	1.0315265458198934E-4	0.0	0.0	5.993272383868162	0.0
8	1.0315265458198934E-4	0.0	0.0	7.513536207097522	0.0
9	1.0315265458198934E-4	0.0	0.0	8.032290906990346	0.0
10	1.0315265458198934E-4	0.0	0.0	9.31633515122695	0.0
11	1.0315265458198934E-4	0.0	0.0	10.918708487503572	0.0
12	1.0315265458198934E-4	0.0	0.0	12.392553616171035	0.0
13	2.0630530916397868E-4	0.0	0.0	12.913268216500917	0.0
14	2.0630530916397868E-4	0.0	0.0	13.11720101460951	0.0
15	2.0630530916397868E-4	0.0	0.0	13.56261417709454	0.0
16	2.0630530916397868E-4	0.0	0.0	14.422494705690003	0.0
17	2.0630530916397868E-4	0.0	0.0	15.421734470625735	0.0
18	4.1261061832795735E-4	0.0	0.0	16.60334812886242	0.0
19	4.1261061832795735E-4	0.0	0.0	17.23474552755878	0.0
20	4.1261061832795735E-4	0.0	0.0	17.838704320136326	0.0
21	0.0012378318549838721	0.0	0.0	18.617300556921183	0.0
22	0.0012378318549838721	0.0	0.0	19.34824026728916	0.0
23	0.0012378318549838721	0.0	0.0	20.116933849234144	0.0
24	0.0014441371641478508	0.0	0.0	20.75709922356997	0.0
25	0.0014441371641478508	0.0	0.0	21.329390151190847	0.0
26	0.0014441371641478508	0.0	0.0	21.858253811232704	0.0
27	0.0014441371641478508	0.0	0.0	22.343587051040966	0.0
28	0.0014441371641478508	0.0	0.0	22.85295485936683	0.0
29	0.0014441371641478508	0.0	0.0	23.40667830916295	0.0
30	0.0014441371641478508	0.0	0.0	24.042408119351748	0.0
31	0.0014441371641478508	0.0	0.0	24.600051370021983	0.0
32	0.0014441371641478508	0.0	0.0	25.140158669413278	0.0
33	0.0014441371641478508	0.0	0.0	25.66706242901808	0.0
34	0.0014441371641478508	0.0	0.0	26.199949042588635	0.0
35	0.0014441371641478508	0.0	0.0	26.75975849900509	0.0
36	0.0014441371641478508	0.0	0.0	27.276862756424606	0.0
37	0.0014441371641478508	0.0	0.0	27.77735943645642	0.0
38	0.0014441371641478508	0.0	0.0	28.24721977807738	0.0
39	0.0014441371641478508	0.0	0.0	28.7367822767235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	7.0327386E-4	45.000004	11
GTTAACG	40	6.8139343E-9	45.000004	1
TATAGCG	40	6.8139343E-9	45.000004	1
TACTAGA	35	1.2116652E-7	45.000004	30
GACGCGA	30	2.1651485E-6	45.000004	23
CCGCACG	30	2.1651485E-6	45.000004	38
GTTGCGT	20	7.0327386E-4	45.000004	35
CAACGTA	25	3.890352E-5	45.0	18
TAGAACG	25	3.890352E-5	45.0	2
CGAATAT	115	0.0	44.999996	14
CGGTCTA	85	0.0	42.35294	31
CACGCTT	55	6.002665E-11	40.909092	13
TACGTAG	55	6.002665E-11	40.909092	1
AGTCACG	50	1.0804797E-9	40.5	1
TAACGCC	45	1.927765E-8	40.0	12
ACGATAG	45	1.927765E-8	40.0	1
TACGTTG	45	1.927765E-8	40.0	1
CAGGTCG	40	3.4574805E-7	39.375004	1
GTATCGC	40	3.4574805E-7	39.375004	5
CGTAAGG	160	0.0	39.375004	2
>>END_MODULE