SRR3549908_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549908_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682432
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1956	0.2866219638000563	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1795	0.26302986964268965	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	1120	0.16411891587733285	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.1356911750914377	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	709	0.10389313514020446	TruSeq Adapter, Index 22 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.10037630122854732	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTA	35	1.2109558E-7	45.000004	41
TGCTCGA	30	2.1642009E-6	45.000004	15
CCACGCG	30	2.1642009E-6	45.000004	23
TAGTACG	30	2.1642009E-6	45.000004	1
TTATGCG	20	7.031269E-4	45.0	1
GCACGTT	25	3.8891336E-5	45.0	23
TGCGACA	25	3.8891336E-5	45.0	38
TGTTACG	25	3.8891336E-5	45.0	1
GGACGTA	20	7.031269E-4	45.0	8
CGTACGA	25	3.8891336E-5	45.0	21
GTAAGCG	20	7.031269E-4	45.0	1
CTCTCGA	20	7.031269E-4	45.0	11
TCGACGT	25	3.8891336E-5	45.0	26
TCGGTAA	20	7.031269E-4	45.0	13
TCGAACG	20	7.031269E-4	45.0	1
GCGTAAG	50	2.1827873E-11	45.0	1
TACGTTG	25	3.8891336E-5	45.0	1
CGAATAT	105	0.0	42.857147	14
AGCTACG	105	0.0	42.857147	9
CGTAAGG	60	3.6379788E-12	41.250004	2