##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549908_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	682432
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5760031768733	34.0	31.0	34.0	31.0	34.0
2	32.761664165807	34.0	31.0	34.0	31.0	34.0
3	32.75976214479977	34.0	31.0	34.0	31.0	34.0
4	36.177894647378785	37.0	37.0	37.0	35.0	37.0
5	36.18326954187377	37.0	35.0	37.0	35.0	37.0
6	36.17749460752133	37.0	36.0	37.0	35.0	37.0
7	36.274639817593545	37.0	37.0	37.0	35.0	37.0
8	36.27617110569258	37.0	37.0	37.0	35.0	37.0
9	37.64658896417519	39.0	38.0	39.0	35.0	39.0
10	37.692007408796776	39.0	37.0	39.0	35.0	39.0
11	37.63526036293726	39.0	37.0	39.0	35.0	39.0
12	37.497182136828286	39.0	37.0	39.0	35.0	39.0
13	37.44427576666979	39.0	37.0	39.0	35.0	39.0
14	38.69044534840101	40.0	38.0	41.0	35.0	41.0
15	38.74698284957329	40.0	38.0	41.0	35.0	41.0
16	38.76857327909594	40.0	38.0	41.0	35.0	41.0
17	38.72805788708619	40.0	38.0	41.0	35.0	41.0
18	38.67377409031229	40.0	38.0	41.0	35.0	41.0
19	38.64165367391916	40.0	38.0	41.0	35.0	41.0
20	38.599857568226575	40.0	38.0	41.0	35.0	41.0
21	38.56948970739942	40.0	38.0	41.0	35.0	41.0
22	38.53166469333208	40.0	38.0	41.0	35.0	41.0
23	38.51762812998218	40.0	38.0	41.0	35.0	41.0
24	38.50313437822376	40.0	38.0	41.0	35.0	41.0
25	38.39940096595705	40.0	37.0	41.0	34.0	41.0
26	38.31796721138517	40.0	37.0	41.0	34.0	41.0
27	38.22767982744068	40.0	37.0	41.0	34.0	41.0
28	38.14284353605927	40.0	37.0	41.0	34.0	41.0
29	38.04823191175092	40.0	37.0	41.0	34.0	41.0
30	37.95577141751852	40.0	36.0	41.0	34.0	41.0
31	37.85080711338273	40.0	36.0	41.0	34.0	41.0
32	37.738507279846196	40.0	36.0	41.0	33.0	41.0
33	37.62051603676264	40.0	36.0	41.0	33.0	41.0
34	37.512760245709465	40.0	36.0	41.0	33.0	41.0
35	37.43991049657695	40.0	35.0	41.0	33.0	41.0
36	37.319769588764885	40.0	35.0	41.0	33.0	41.0
37	37.215712334708805	40.0	35.0	41.0	32.0	41.0
38	37.048645139735534	39.0	35.0	41.0	32.0	41.0
39	36.95131236518803	39.0	35.0	41.0	32.0	41.0
40	36.80710019459814	39.0	35.0	41.0	31.0	41.0
41	36.75981929335084	39.0	35.0	41.0	31.0	41.0
42	36.650842867860824	39.0	35.0	41.0	31.0	41.0
43	36.54429452311732	39.0	35.0	41.0	31.0	41.0
44	36.40242397777361	39.0	35.0	41.0	31.0	41.0
45	36.26725007033668	39.0	35.0	41.0	31.0	41.0
46	36.146610944387135	38.0	35.0	40.0	30.0	41.0
47	36.04675484150802	38.0	35.0	40.0	30.0	41.0
48	35.89853348025884	38.0	35.0	40.0	30.0	41.0
49	35.78404295226484	38.0	35.0	40.0	29.0	41.0
50	35.63089069680203	37.0	35.0	40.0	29.0	41.0
51	33.89800302447716	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	24.0
10	20.0
11	27.0
12	13.0
13	11.0
14	16.0
15	37.0
16	49.0
17	87.0
18	171.0
19	323.0
20	491.0
21	784.0
22	1300.0
23	1792.0
24	2519.0
25	3480.0
26	4226.0
27	4971.0
28	5977.0
29	7046.0
30	8433.0
31	10677.0
32	13948.0
33	19800.0
34	39647.0
35	54162.0
36	46770.0
37	74933.0
38	143659.0
39	236922.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.918022601519276	21.163866876113662	25.7631529588296	11.154957563537465
2	31.446942699052798	24.755726577886147	25.57910531745288	18.218225405608177
3	32.01417870205383	24.79089491700272	26.543450482978525	16.651475897964925
4	30.007971490199758	25.69662618400075	25.061984197692954	19.233418128106536
5	25.179065460001876	30.690823408046512	23.896300290724938	20.23381084122667
6	26.57246436274969	35.43620463284254	22.993939322892242	14.99739168151552
7	80.95487902091344	6.491782331426428	7.570131529588296	4.983207118071837
8	82.1878223764419	5.440835130826222	7.303731360780268	5.067611131951608
9	75.18741794054206	8.014864484666605	9.588208055894214	7.209509518897121
10	41.04804581262309	26.757391212604333	16.467721326080838	15.726841648691739
11	31.771517162149486	24.825916721372973	22.602984619713027	20.799581496764514
12	29.07205406546	22.46861225733846	28.43228336303104	20.0270503141705
13	25.395936884554065	24.43276399699897	29.491289974678796	20.68000914376817
14	20.414927787677016	28.70337264372128	28.062283128575448	22.81941644002626
15	20.157905842633404	25.983101613054487	33.1256447528838	20.7333477914283
16	21.87924950764325	24.197429194410578	31.48987737972428	22.433443918221887
17	21.74297219356654	24.48493036668855	27.123288474162994	26.648808965581917
18	23.21447411610241	25.09685946731689	28.807265778861485	22.881400637719214
19	24.76774242708431	26.02193332082903	26.293022601519272	22.91730165056738
20	26.719438713307696	25.45147355340898	25.75992919441058	22.06915853887274
21	25.66629349151271	25.605335037043986	27.231577651692767	21.49679381975054
22	22.829380802775955	25.218190237269063	25.964784769764606	25.98764419019038
23	23.804716074275532	26.040396698865237	26.082745240551443	24.072141986307795
24	24.464562036950202	24.499144237081495	27.4733599831192	23.562933742849104
25	23.18531370158492	25.75978265966426	26.041715511582108	25.01318812716871
26	22.713911422676546	26.672107990246648	26.11835318390697	24.49562740316984
27	23.631951608365377	26.729403076057395	26.492309856513174	23.146335459064055
28	21.788104895432806	25.96874120791522	28.31549517021476	23.927658726437212
29	23.44995545343712	25.50334685360593	26.850587311263247	24.196110381693707
30	23.970739941855012	25.223758557629182	26.76632983212979	24.039171668386007
31	25.426416111788424	25.999073900403264	24.68187306574135	23.89263692206696
32	24.79279986870487	26.794611038169368	25.66746576948326	22.745123323642503
33	24.477457094626278	26.86846455031417	25.028720810278532	23.625357544781018
34	23.836660648973083	24.885116758885868	27.421926287161213	23.85629630497984
35	23.789476460658353	23.563373347088064	27.265573712838787	25.381576479414797
36	25.62892713120135	26.433989027478194	25.231671433930416	22.705412407390043
37	23.876664634718185	25.64739050923755	28.867051955359656	21.60889290068461
38	22.85018873675326	27.353934164869177	26.892496248710497	22.903380849667073
39	24.50940166932383	24.835001875644753	27.219708337240927	23.43588811779049
40	23.966050829972804	24.422799634249273	29.724280221326083	21.886869314451843
41	21.580318625152394	24.582522507737036	28.48957844884179	25.347580418268777
42	23.62022882865985	24.368728312857545	27.70210072212323	24.308942136359374
43	25.357398246272155	23.85717551345775	26.719438713307696	24.06598752696239
44	23.44189604238957	24.851853371471442	27.145268686110853	24.560981900028132
45	22.99174130169746	24.05851425490012	27.107462721560537	25.842281721841882
46	23.59033574041077	24.917940542061334	27.84101566163369	23.650708055894214
47	23.182822610897496	24.154347978992778	29.946280361999438	22.716549048110288
48	22.676984666604145	23.541832739379164	29.495392947575727	24.285789646440964
49	23.107210681796868	23.696280361999435	29.251705664447154	23.944803291756543
50	21.979186204632843	23.056216590077838	29.671674247397544	25.29292295789178
51	21.973764419019037	23.535531745287443	27.390860920941574	27.09984291475195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	381.0
1	729.5
2	1078.0
3	1539.5
4	2001.0
5	1509.0
6	1017.0
7	942.5
8	868.0
9	937.5
10	1007.0
11	1019.0
12	1031.0
13	1117.0
14	1203.0
15	1236.0
16	1269.0
17	1234.0
18	1199.0
19	1407.5
20	1616.0
21	1787.5
22	1959.0
23	2184.5
24	2410.0
25	2900.5
26	4312.5
27	5234.0
28	6699.0
29	8164.0
30	9104.5
31	10045.0
32	11273.0
33	12501.0
34	13793.0
35	15085.0
36	16025.5
37	16966.0
38	19697.0
39	22428.0
40	25056.0
41	27684.0
42	30862.5
43	34041.0
44	38532.5
45	43024.0
46	49380.5
47	55737.0
48	60294.5
49	64852.0
50	64325.5
51	63799.0
52	55799.5
53	47800.0
54	42614.0
55	37428.0
56	36433.0
57	35438.0
58	34181.5
59	32925.0
60	32671.0
61	32417.0
62	30446.5
63	28476.0
64	25198.0
65	21920.0
66	18390.0
67	14860.0
68	12859.0
69	10858.0
70	9478.5
71	8099.0
72	6413.0
73	4727.0
74	4246.0
75	2654.5
76	1544.0
77	1347.0
78	1150.0
79	823.0
80	496.0
81	384.0
82	272.0
83	185.5
84	99.0
85	80.0
86	61.0
87	49.0
88	37.0
89	31.0
90	25.0
91	27.5
92	30.0
93	21.5
94	13.0
95	7.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	682432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.645716927795107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94844308123425	19.890662908193804
2	10.709880952330598	5.921646742770328
3	3.997314596832821	3.3152588344615084
4	1.9233896593527156	2.1269394425725374
5	1.2014545142374469	1.6607535701115017
6	0.8230087736746711	1.3651600551661047
7	0.6346809179807283	1.228234629857785
8	0.50860260944344	1.1248547015529018
9	0.42730429697106626	1.0631820272483334
>10	7.290424427874967	49.79658034917896
>50	0.46295884025737993	7.728841282560641
>100	0.06667033989880046	3.306904827608977
>500	0.004266901753523229	0.7527421922211543
>1k	0.0016000881575712112	0.7182384364954443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1956	0.2866219638000563	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1795	0.26302986964268965	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	1120	0.16411891587733285	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.1356911750914377	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	709	0.10389313514020446	TruSeq Adapter, Index 22 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.10037630122854732	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.396042389571415E-4	0.0	0.0	0.186392197317828	0.0
2	4.396042389571415E-4	0.0	0.0	1.0200283691268874	0.0
3	4.396042389571415E-4	0.0	0.0	1.469450436087405	0.0
4	4.396042389571415E-4	0.0	0.0	1.9220962674669417	0.0
5	4.396042389571415E-4	0.0	0.0	3.2354871987245617	0.0
6	4.396042389571415E-4	0.0	0.0	3.9523351777173406	0.0
7	4.396042389571415E-4	0.0	0.0	4.680466332176686	0.0
8	4.396042389571415E-4	0.0	0.0	5.802043280502673	0.0
9	4.396042389571415E-4	0.0	0.0	6.200910859983119	0.0
10	4.396042389571415E-4	0.0	0.0	7.1337510550501735	0.0
11	4.396042389571415E-4	0.0	0.0	8.376365703835694	0.0
12	4.396042389571415E-4	0.0	0.0	9.393316843289881	0.0
13	4.396042389571415E-4	0.0	0.0	9.78573689393229	0.0
14	5.861389852761887E-4	0.0	0.0	9.937107286879865	0.0
15	5.861389852761887E-4	0.0	0.0	10.25010550501735	0.0
16	5.861389852761887E-4	0.0	0.0	10.911709884647848	0.0
17	5.861389852761887E-4	0.0	0.0	11.72966683860077	0.0
18	5.861389852761887E-4	0.0	0.0	12.6754020913439	0.0
19	5.861389852761887E-4	0.0	0.0	13.184610334802588	0.0
20	5.861389852761887E-4	0.0	0.0	13.677992825658821	0.0
21	5.861389852761887E-4	0.0	0.0	14.308824908562318	0.0
22	5.861389852761887E-4	0.0	0.0	14.950354027947107	0.0
23	5.861389852761887E-4	0.0	0.0	15.595106911750914	0.0
24	5.861389852761887E-4	0.0	0.0	16.124390415455313	0.0
25	5.861389852761887E-4	0.0	0.0	16.594913485885773	0.0
26	5.861389852761887E-4	0.0	0.0	17.01546820782144	0.0
27	5.861389852761887E-4	0.0	0.0	17.45990809340711	0.0
28	5.861389852761887E-4	0.0	0.0	17.9106489730845	0.0
29	5.861389852761887E-4	0.0	0.0	18.398756213073245	0.0
30	5.861389852761887E-4	0.0	0.0	18.94181398293163	0.0
31	5.861389852761887E-4	0.0	0.0	19.4392994466848	0.0
32	5.861389852761887E-4	0.0	0.0	19.90337498827722	0.0
33	5.861389852761887E-4	0.0	0.0	20.366571321391728	0.0
34	5.861389852761887E-4	0.0	0.0	20.82654389008722	0.0
35	5.861389852761887E-4	0.0	0.0	21.35509472006002	0.0
36	5.861389852761887E-4	0.0	0.0	21.841736612585578	0.0
37	5.861389852761887E-4	0.0	0.0	22.34566960517678	0.0
38	5.861389852761887E-4	0.0	0.0	22.80417682640908	0.0
39	7.326737315952359E-4	0.0	0.0	23.265907812060394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTA	35	1.2109558E-7	45.000004	41
TGCTCGA	30	2.1642009E-6	45.000004	15
CCACGCG	30	2.1642009E-6	45.000004	23
TAGTACG	30	2.1642009E-6	45.000004	1
TTATGCG	20	7.031269E-4	45.0	1
GCACGTT	25	3.8891336E-5	45.0	23
TGCGACA	25	3.8891336E-5	45.0	38
TGTTACG	25	3.8891336E-5	45.0	1
GGACGTA	20	7.031269E-4	45.0	8
CGTACGA	25	3.8891336E-5	45.0	21
GTAAGCG	20	7.031269E-4	45.0	1
CTCTCGA	20	7.031269E-4	45.0	11
TCGACGT	25	3.8891336E-5	45.0	26
TCGGTAA	20	7.031269E-4	45.0	13
TCGAACG	20	7.031269E-4	45.0	1
GCGTAAG	50	2.1827873E-11	45.0	1
TACGTTG	25	3.8891336E-5	45.0	1
CGAATAT	105	0.0	42.857147	14
AGCTACG	105	0.0	42.857147	9
CGTAAGG	60	3.6379788E-12	41.250004	2
>>END_MODULE