SRR3549906_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	503869
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1578	0.3131766391661325	RNA PCR Primer, Index 40 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	1576	0.312779710599382	RNA PCR Primer, Index 46 (96% over 26bp)
TGAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC	1180	0.234187854382786	No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	948	0.18814414063972978	RNA PCR Primer, Index 46 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	644	0.1278109984936561	No Hit
TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC	621	0.1232463199760255	RNA PCR Primer, Index 40 (95% over 23bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	537	0.10657532017250516	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	20	7.02951E-4	45.000004	1
TCGCACC	20	7.02951E-4	45.000004	43
GTCGACG	25	3.8876755E-5	45.000004	1
CACGGTT	25	3.8876755E-5	45.000004	4
CAACGAC	20	7.02951E-4	45.000004	12
GTTAGCG	20	7.02951E-4	45.000004	1
CCTAACC	25	3.8876755E-5	45.000004	7
AGTATGA	25	3.8876755E-5	45.000004	31
ATCGTCC	20	7.02951E-4	45.000004	19
TCGTGGC	20	7.02951E-4	45.000004	20
TGATTCG	20	7.02951E-4	45.000004	15
CTACGCC	20	7.02951E-4	45.000004	30
TACGAAG	20	7.02951E-4	45.000004	1
AACGCAC	20	7.02951E-4	45.000004	24
ACTACGC	20	7.02951E-4	45.000004	29
TAGATTG	25	3.8876755E-5	45.000004	1
CGTGTAT	25	3.8876755E-5	45.000004	18
CGCACAT	20	7.02951E-4	45.000004	26
CCGTTCG	20	7.02951E-4	45.000004	17
TACGTTG	25	3.8876755E-5	45.000004	1