##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549906_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 503869 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52454705488927 33.0 31.0 34.0 31.0 34.0 2 32.69943576604236 34.0 31.0 34.0 31.0 34.0 3 32.7193298258079 34.0 31.0 34.0 31.0 34.0 4 36.14947734430973 37.0 35.0 37.0 35.0 37.0 5 36.14813374111128 37.0 35.0 37.0 35.0 37.0 6 36.1343404734167 37.0 36.0 37.0 35.0 37.0 7 36.218727486707856 37.0 37.0 37.0 35.0 37.0 8 36.21672696673143 37.0 37.0 37.0 35.0 37.0 9 37.59342011514898 39.0 38.0 39.0 35.0 39.0 10 37.61794831593132 39.0 37.0 39.0 35.0 39.0 11 37.560606030535716 39.0 37.0 39.0 35.0 39.0 12 37.394558903206985 39.0 37.0 39.0 35.0 39.0 13 37.28440725664806 39.0 37.0 39.0 34.0 39.0 14 38.510098855059546 40.0 38.0 41.0 34.0 41.0 15 38.59076069375175 40.0 38.0 41.0 35.0 41.0 16 38.61931771948661 40.0 38.0 41.0 35.0 41.0 17 38.5739289378787 40.0 38.0 41.0 35.0 41.0 18 38.529891698040565 40.0 38.0 41.0 35.0 41.0 19 38.51301628002516 40.0 37.0 41.0 35.0 41.0 20 38.448362570430014 40.0 37.0 41.0 34.0 41.0 21 38.42787113317152 40.0 37.0 41.0 34.0 41.0 22 38.384679351180566 40.0 37.0 41.0 34.0 41.0 23 38.362308457158505 40.0 37.0 41.0 34.0 41.0 24 38.3234114422598 40.0 37.0 41.0 34.0 41.0 25 38.24615723531315 40.0 37.0 41.0 34.0 41.0 26 38.17125284548166 40.0 37.0 41.0 34.0 41.0 27 38.08079480976206 40.0 36.0 41.0 34.0 41.0 28 37.9902157108296 40.0 36.0 41.0 34.0 41.0 29 37.93235543365438 40.0 36.0 41.0 34.0 41.0 30 37.79570285133636 40.0 36.0 41.0 33.0 41.0 31 37.67739233808788 40.0 36.0 41.0 33.0 41.0 32 37.578769481750214 40.0 36.0 41.0 33.0 41.0 33 37.46007593243482 40.0 35.0 41.0 33.0 41.0 34 37.32991114754033 40.0 35.0 41.0 33.0 41.0 35 37.28532416163725 40.0 35.0 41.0 33.0 41.0 36 37.174225840446624 40.0 35.0 41.0 32.0 41.0 37 37.067906539199676 39.0 35.0 41.0 32.0 41.0 38 36.909925000347314 39.0 35.0 41.0 32.0 41.0 39 36.78161784114522 39.0 35.0 41.0 31.0 41.0 40 36.6367627299953 39.0 35.0 41.0 31.0 41.0 41 36.58612258344927 39.0 35.0 41.0 31.0 41.0 42 36.4748158747611 39.0 35.0 41.0 31.0 41.0 43 36.41535399081904 39.0 35.0 41.0 31.0 41.0 44 36.271098241804914 39.0 35.0 41.0 30.0 41.0 45 36.127807029207986 38.0 35.0 40.0 30.0 41.0 46 36.00459643280297 38.0 35.0 40.0 30.0 41.0 47 35.91220733960613 38.0 35.0 40.0 30.0 41.0 48 35.7919657688804 38.0 35.0 40.0 29.0 41.0 49 35.68916127009203 38.0 35.0 40.0 29.0 41.0 50 35.587430463076714 38.0 35.0 40.0 29.0 41.0 51 33.87017855831575 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 2.0 9 15.0 10 19.0 11 10.0 12 11.0 13 18.0 14 15.0 15 15.0 16 37.0 17 78.0 18 156.0 19 266.0 20 428.0 21 690.0 22 987.0 23 1435.0 24 2086.0 25 2698.0 26 3357.0 27 3932.0 28 4488.0 29 5362.0 30 6628.0 31 8581.0 32 10776.0 33 15385.0 34 31169.0 35 44072.0 36 35003.0 37 55604.0 38 101753.0 39 168710.0 40 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46361653525023 20.41244847371043 25.510400520770283 11.613534470269057 2 31.438925593755517 24.72487888717107 25.976196193851976 17.859999325221438 3 31.657434769751664 25.565573591548596 25.388940379344632 17.388051259355112 4 28.393292701079048 26.847057469302538 25.56577205583197 19.19387777378644 5 25.311142380261536 31.223988774860135 24.20986407181232 19.255004773066016 6 26.223284226654147 35.422103761096636 23.000621193206964 15.353990819042252 7 80.78706965500953 7.031788024268212 7.569229303648369 4.611913017073882 8 81.54639400320322 5.585380327029446 7.6914833022075175 5.1767423675598225 9 74.64380622741228 8.274968295330732 10.082779452595814 6.998446024661171 10 39.40984660695538 27.363263070361544 16.7251805528818 16.50170976980128 11 32.29589436936982 25.0372616692037 22.494338806316723 20.17250515510976 12 28.32482252331459 21.84913142106381 29.24470447675884 20.581341578862762 13 24.042955609493738 24.58396924597465 30.4378717484108 20.935203396120816 14 20.592852507298524 28.361736880022388 28.875560909680893 22.1698497029982 15 20.372557152752005 25.194842310203647 33.87884549357075 20.5537550434736 16 22.017826061932762 24.23348132153397 30.895728850157482 22.852963766375783 17 21.172169750470857 24.250350785620867 27.164401858419552 27.413077605488727 18 22.79679837418059 24.965020670055114 29.02063830082819 23.217542654936103 19 23.758953219983766 25.239298309679697 26.694438435386974 24.307310034949563 20 24.952318955919097 25.371872450974358 26.771244113053193 22.904564480053345 21 24.816172457523685 26.03216312176379 27.41883306970661 21.732831351005917 22 22.692406161125213 23.79626450525831 26.656730221545676 26.854599112070797 23 22.39133584324497 26.14231079903705 25.950594301296565 25.515759056421413 24 22.96192065794879 24.70066624459929 28.283938880939292 24.053474216512623 25 22.129164524906276 25.907527551804137 26.01132437200939 25.951983551280193 26 20.880625718192626 26.67379814991595 26.647402400227044 25.79817373166438 27 22.168658917297947 26.124647477816655 26.865713111939808 24.840980492945587 28 20.365809367117247 25.776144196209728 28.388529558278048 25.469516878394984 29 22.055732740057437 24.5859538888084 26.7535807918328 26.604732579301366 30 23.38881733148894 25.88748265918324 25.04817720478934 25.67552280453848 31 23.649202471277256 27.08759618075333 23.09965487061121 26.163546477358203 32 24.108845751574318 26.45032736683543 23.579938436379297 25.860888445210957 33 23.136966155885755 27.06338353818155 23.14669090577114 26.652959400161553 34 20.660727292212858 26.39316965322336 26.02343069329528 26.922672361268507 35 22.035290918869784 24.528994639479706 25.78507508896162 27.650639352688895 36 22.663430375752426 27.454556640714156 24.63279145968496 25.24922152384846 37 22.167666595881073 27.618686603065477 26.34097354669567 23.872673254357778 38 22.183742202834466 28.991464051172034 24.622272852666068 24.20252089332743 39 23.18439911961244 27.302731463932094 25.566565912965473 23.946303503489993 40 22.892458158767457 25.33277498714944 28.07396366912829 23.700803184954818 41 19.29608687972469 26.890322683078338 26.208994798251133 27.60459563894584 42 20.72860207712719 25.794601374563626 26.669431935681697 26.807364612627488 43 22.915480015638988 23.914549218149954 27.20449164366135 25.965479122549713 44 22.224427380926393 24.638348459619465 25.727718911066173 27.409505248387973 45 22.26868491611907 25.471303056945356 25.29526523759152 26.96474678934406 46 22.697764696776346 25.740817553768935 26.93279403972064 24.628623709734075 47 21.557785853068953 25.054528061857344 28.571712091833394 24.815973993240306 48 21.019550716555297 24.37240631989664 28.186095989235298 26.421946974312764 49 21.74851002939256 24.387291141149785 28.91723840918969 24.946960420267967 50 20.740113005562954 23.35607072473202 29.60729872248541 26.296517547219615 51 20.94810357454021 24.50220196122405 25.932732515792793 28.61696194844295 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 325.0 1 624.5 2 924.0 3 1165.0 4 1406.0 5 1062.0 6 718.0 7 702.5 8 687.0 9 713.5 10 740.0 11 749.0 12 758.0 13 792.0 14 826.0 15 874.0 16 922.0 17 906.0 18 890.0 19 918.0 20 946.0 21 1028.0 22 1110.0 23 1500.5 24 1891.0 25 1941.0 26 2594.0 27 3197.0 28 4209.5 29 5222.0 30 6095.5 31 6969.0 32 7863.5 33 8758.0 34 9420.5 35 10083.0 36 10755.0 37 11427.0 38 12849.0 39 14271.0 40 16769.5 41 19268.0 42 22751.5 43 26235.0 44 30938.0 45 35641.0 46 40077.0 47 44513.0 48 47444.5 49 50376.0 50 49645.5 51 48915.0 52 43593.0 53 38271.0 54 33190.0 55 28109.0 56 27139.0 57 26169.0 58 24670.0 59 23171.0 60 22467.0 61 21763.0 62 20438.5 63 19114.0 64 17283.0 65 15452.0 66 13080.0 67 10708.0 68 8934.5 69 7161.0 70 6235.0 71 5309.0 72 4497.0 73 3685.0 74 2872.5 75 1906.5 76 1753.0 77 1349.5 78 946.0 79 792.0 80 638.0 81 431.0 82 224.0 83 173.0 84 122.0 85 111.0 86 100.0 87 68.5 88 37.0 89 34.0 90 31.0 91 32.0 92 33.0 93 18.5 94 4.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 503869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.319235295658284 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86711130963305 20.635408704924213 2 10.53401537583226 5.966305200725521 3 3.9003897395465468 3.3136816433697 4 1.963943443892098 2.2246950597978317 5 1.153852654392452 1.6338112408129866 6 0.7713161023302679 1.310584931353254 7 0.606154168575904 1.20160757677416 8 0.504179607203449 1.1422384742133633 9 0.42297269905005536 1.0780437049234362 >10 6.688935872457846 47.710940847574555 >50 0.5117114929946033 8.68259103682811 >100 0.07048367672101974 3.6860941326721233 >500 0.0028193470688407897 0.5489120520305594 >1k 0.0021145103016305925 0.8650853940001616 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 1578 0.3131766391661325 RNA PCR Primer, Index 40 (95% over 23bp) CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 1576 0.312779710599382 RNA PCR Primer, Index 46 (96% over 26bp) TGAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC 1180 0.234187854382786 No Hit CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT 948 0.18814414063972978 RNA PCR Primer, Index 46 (96% over 27bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 0.1278109984936561 No Hit TCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 621 0.1232463199760255 RNA PCR Primer, Index 40 (95% over 23bp) AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 537 0.10657532017250516 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.9846428337524238E-4 0.0 0.0 0.21414296176188652 0.0 2 1.9846428337524238E-4 0.0 0.0 1.1687561647968023 0.0 3 1.9846428337524238E-4 0.0 0.0 1.6101407310233413 0.0 4 1.9846428337524238E-4 0.0 0.0 2.1055075823279465 0.0 5 1.9846428337524238E-4 0.0 0.0 3.5584646009180956 0.0 6 1.9846428337524238E-4 0.0 0.0 4.387052984009733 0.0 7 1.9846428337524238E-4 0.0 0.0 5.215045974251244 0.0 8 1.9846428337524238E-4 0.0 0.0 6.649942743054246 0.0 9 1.9846428337524238E-4 0.0 0.0 7.1564235942278644 0.0 10 1.9846428337524238E-4 0.0 0.0 8.273777509630479 0.0 11 1.9846428337524238E-4 0.0 0.0 9.86605645515005 0.0 12 1.9846428337524238E-4 0.0 0.0 11.029851012862471 0.0 13 1.9846428337524238E-4 0.0 0.0 11.561139899457993 0.0 14 1.9846428337524238E-4 0.0 0.0 11.735590004544832 0.0 15 1.9846428337524238E-4 0.0 0.0 12.099771964538402 0.0 16 1.9846428337524238E-4 0.0 0.0 12.858103991315202 0.0 17 1.9846428337524238E-4 0.0 0.0 13.768658123440815 0.0 18 1.9846428337524238E-4 0.0 0.0 14.984251859114174 0.0 19 1.9846428337524238E-4 0.0 0.0 15.502243638723558 0.0 20 1.9846428337524238E-4 0.0 0.0 16.058935953591114 0.0 21 1.9846428337524238E-4 0.0 0.0 16.694418588958637 0.0 22 1.9846428337524238E-4 0.0 0.0 17.31005479598864 0.0 23 1.9846428337524238E-4 0.0 0.0 17.951094431290674 0.0 24 1.9846428337524238E-4 0.0 0.0 18.469086210900056 0.0 25 1.9846428337524238E-4 0.0 0.0 18.961277633670658 0.0 26 1.9846428337524238E-4 0.0 0.0 19.371106378840533 0.0 27 1.9846428337524238E-4 0.0 0.0 19.812689409350448 0.0 28 1.9846428337524238E-4 0.0 0.0 20.244547689974972 0.0 29 1.9846428337524238E-4 0.0 0.0 20.714114184440795 0.0 30 1.9846428337524238E-4 0.0 0.0 21.28390514201112 0.0 31 1.9846428337524238E-4 0.0 0.0 21.746326922275433 0.0 32 1.9846428337524238E-4 0.0 0.0 22.239709130746284 0.0 33 1.9846428337524238E-4 0.0 0.0 22.686055304057206 0.0 34 1.9846428337524238E-4 0.0 0.0 23.15145404857215 0.0 35 1.9846428337524238E-4 0.0 0.0 23.655156399778512 0.0 36 1.9846428337524238E-4 0.0 0.0 24.110631930124693 0.0 37 3.9692856675048476E-4 0.0 0.0 24.550230317800857 0.0 38 3.9692856675048476E-4 0.0 0.0 24.975340812790627 0.0 39 3.9692856675048476E-4 0.0 0.0 25.415336129033538 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTAG 20 7.02951E-4 45.000004 1 TCGCACC 20 7.02951E-4 45.000004 43 GTCGACG 25 3.8876755E-5 45.000004 1 CACGGTT 25 3.8876755E-5 45.000004 4 CAACGAC 20 7.02951E-4 45.000004 12 GTTAGCG 20 7.02951E-4 45.000004 1 CCTAACC 25 3.8876755E-5 45.000004 7 AGTATGA 25 3.8876755E-5 45.000004 31 ATCGTCC 20 7.02951E-4 45.000004 19 TCGTGGC 20 7.02951E-4 45.000004 20 TGATTCG 20 7.02951E-4 45.000004 15 CTACGCC 20 7.02951E-4 45.000004 30 TACGAAG 20 7.02951E-4 45.000004 1 AACGCAC 20 7.02951E-4 45.000004 24 ACTACGC 20 7.02951E-4 45.000004 29 TAGATTG 25 3.8876755E-5 45.000004 1 CGTGTAT 25 3.8876755E-5 45.000004 18 CGCACAT 20 7.02951E-4 45.000004 26 CCGTTCG 20 7.02951E-4 45.000004 17 TACGTTG 25 3.8876755E-5 45.000004 1 >>END_MODULE