##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977950
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64083848867529	34.0	31.0	34.0	31.0	34.0
2	32.827538217700294	34.0	31.0	34.0	31.0	34.0
3	32.81078889513779	34.0	31.0	34.0	31.0	34.0
4	36.219708574057975	37.0	37.0	37.0	35.0	37.0
5	36.225641392709235	37.0	37.0	37.0	35.0	37.0
6	36.220187126131194	37.0	37.0	37.0	35.0	37.0
7	36.314304412291015	37.0	37.0	37.0	35.0	37.0
8	36.31247712050718	37.0	37.0	37.0	35.0	37.0
9	37.704080985735466	39.0	38.0	39.0	35.0	39.0
10	37.717428293880054	39.0	37.0	39.0	35.0	39.0
11	37.66970601769007	39.0	37.0	39.0	35.0	39.0
12	37.55348739710619	39.0	37.0	39.0	35.0	39.0
13	37.50475791195869	39.0	37.0	39.0	35.0	39.0
14	38.801665729331766	40.0	38.0	41.0	35.0	41.0
15	38.83101896824991	40.0	38.0	41.0	35.0	41.0
16	38.843381563474615	40.0	38.0	41.0	35.0	41.0
17	38.78143667876681	40.0	38.0	41.0	35.0	41.0
18	38.72561276138862	40.0	38.0	41.0	35.0	41.0
19	38.696856690014826	40.0	38.0	41.0	35.0	41.0
20	38.671207116928265	40.0	38.0	41.0	35.0	41.0
21	38.63869420727031	40.0	38.0	41.0	35.0	41.0
22	38.601519505087175	40.0	38.0	41.0	35.0	41.0
23	38.56007260084871	40.0	38.0	41.0	35.0	41.0
24	38.54619050053684	40.0	38.0	41.0	35.0	41.0
25	38.45297919116519	40.0	37.0	41.0	35.0	41.0
26	38.36616289176338	40.0	37.0	41.0	34.0	41.0
27	38.29203947032057	40.0	37.0	41.0	34.0	41.0
28	38.18738994836137	40.0	37.0	41.0	34.0	41.0
29	38.107904289585356	40.0	37.0	41.0	34.0	41.0
30	38.012829899279104	40.0	37.0	41.0	34.0	41.0
31	37.91946009509689	40.0	36.0	41.0	34.0	41.0
32	37.81702336520272	40.0	36.0	41.0	33.0	41.0
33	37.682557390459635	40.0	36.0	41.0	33.0	41.0
34	37.549624213916864	40.0	36.0	41.0	33.0	41.0
35	37.48205736489596	40.0	36.0	41.0	33.0	41.0
36	37.38102152461782	40.0	35.0	41.0	33.0	41.0
37	37.28909351193824	40.0	35.0	41.0	33.0	41.0
38	37.14502581931592	40.0	35.0	41.0	32.0	41.0
39	37.03200879390562	39.0	35.0	41.0	32.0	41.0
40	36.892299197300474	39.0	35.0	41.0	31.0	41.0
41	36.781511324709854	39.0	35.0	41.0	31.0	41.0
42	36.654148985121935	39.0	35.0	41.0	31.0	41.0
43	36.597307633314585	39.0	35.0	41.0	31.0	41.0
44	36.49183802852907	39.0	35.0	41.0	31.0	41.0
45	36.357067334730814	39.0	35.0	41.0	31.0	41.0
46	36.19806431821668	38.0	35.0	41.0	30.0	41.0
47	36.121580857917074	38.0	35.0	40.0	30.0	41.0
48	35.983128994324865	38.0	35.0	40.0	30.0	41.0
49	35.87166112786952	38.0	35.0	40.0	30.0	41.0
50	35.75336980418222	38.0	35.0	40.0	29.0	41.0
51	34.09793343217956	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	32.0
10	36.0
11	36.0
12	21.0
13	18.0
14	25.0
15	40.0
16	57.0
17	135.0
18	250.0
19	458.0
20	731.0
21	1094.0
22	1754.0
23	2469.0
24	3482.0
25	4782.0
26	5946.0
27	7314.0
28	8401.0
29	9820.0
30	11851.0
31	14838.0
32	19070.0
33	27572.0
34	56624.0
35	75014.0
36	66369.0
37	103974.0
38	195877.0
39	359617.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38734086609745	20.892683675034508	25.14361674932256	11.57635870954548
2	32.05378598087837	24.343166828569966	25.621862058387446	17.98118513216422
3	32.24050309320518	24.26115854593793	26.137430338974387	17.36090802188251
4	29.226852088552587	25.649061812976125	25.77115394447569	19.352932153995603
5	25.27429827700803	30.940845646505444	24.309116007975867	19.47574006851066
6	27.101794570274556	35.090751060892686	22.984303901017434	14.823150467815328
7	82.95649061812976	5.939874226698707	6.84932767523902	4.254307479932512
8	84.17894575387291	4.7272355437394555	6.7992228641546095	4.294595838233038
9	77.30333861649369	7.383097295362749	8.841863080934607	6.471701007208957
10	40.98399713686794	27.581778209519914	15.741500076691036	15.69272457692111
11	31.917991717367965	25.686384784498184	22.167288716192036	20.228334781941818
12	29.637609284728256	21.914617311723504	27.629633416841354	20.81813998670689
13	25.89273480239276	23.39403855002812	29.156705353034408	21.556521294544712
14	21.778516284063603	26.560458101129914	28.569661025614806	23.091364589191677
15	21.585050360447873	25.284830512807403	31.510711181553248	21.619407945191472
16	23.995296283041057	23.906743698553097	29.875044736438465	22.22291528196738
17	23.390868653816657	24.357789253029296	27.62973567155785	24.621606421596194
18	25.03307940078736	24.250012781839562	27.70059819009152	23.016309627281558
19	25.38964159721867	26.32527225318268	25.444859144128024	22.840227005470627
20	28.08231504678153	24.74625492100823	25.195357635870952	21.97607239633928
21	26.391022035891403	26.002760877345466	25.569200879390564	22.037016207372563
22	24.70044480801677	24.335293215399563	25.389334833069178	25.574927143514493
23	24.491742931642722	25.393731785878625	26.20747481977606	23.907050462702593
24	24.65197607239634	23.77943657651209	27.419193210286824	24.149394140804745
25	24.42732246024848	25.131448438059206	24.940232118206453	25.500996983485862
26	23.43575847435963	25.773096784089166	25.535865841811955	25.255278899739253
27	23.911651924945037	25.20926427731479	26.19377268776522	24.685311109974947
28	22.413006799938646	26.479574620379363	27.18993813589652	23.91748044378547
29	24.105015593844268	25.282887673193926	25.802034868858325	24.81006186410348
30	24.149905414387238	24.617720742369244	27.354056955877088	23.87831688736643
31	24.948617004959353	25.454266578045914	25.26560662610563	24.331509790889104
32	26.070146735518172	25.40947901221944	25.276547880771	23.243826371491387
33	24.24490004601462	25.866148576103072	25.79651311416739	24.092438263714914
34	23.06559640063398	25.0981134004806	27.77616442558413	24.060125773301294
35	24.31555805511529	24.27148627230431	26.535916969170202	24.877038703410197
36	23.79733115189938	28.146326499309783	25.456516181808887	22.599826166981952
37	24.34122398895649	27.185132164221077	26.202157574518125	22.271486272304312
38	24.57773914821821	26.885934863745593	25.804284472621298	22.7320415154149
39	24.605347921672887	24.98031596707398	26.14233856536633	24.271997545886805
40	25.128176287131243	23.620839511222457	27.962677028477938	23.288307173168363
41	21.71583414284984	25.724525793752235	27.039521447926784	25.520118615471137
42	22.967227363362134	26.352983281353854	26.22332430083338	24.45646505445064
43	24.037731990388057	24.538575591799173	27.25548340917225	24.168209008640524
44	23.174804437854696	24.641852855462957	27.092182626923666	25.09116007975868
45	23.90909555703257	23.805409274502786	26.46904238457999	25.816452783884657
46	23.664502275167443	25.29229510711182	26.511989365509486	24.53121325221126
47	22.807403241474514	25.209877805613782	28.119535763587095	23.86318318932461
48	22.75177667569917	24.438672733779846	28.15430236719669	24.6552482233243
49	23.057416023314076	24.791553760417198	28.281507234521193	23.869522981747533
50	21.825860217802546	24.111048622117696	28.653407638427325	25.409683521652436
51	22.331305281456107	23.123983843754793	27.02520578761695	27.519505087172146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	390.0
1	955.0
2	1520.0
3	2784.5
4	4049.0
5	2913.5
6	1778.0
7	1611.0
8	1444.0
9	1425.0
10	1406.0
11	1427.0
12	1448.0
13	1440.0
14	1432.0
15	1447.5
16	1463.0
17	1577.5
18	1692.0
19	1907.0
20	2122.0
21	2186.0
22	2250.0
23	2730.5
24	3211.0
25	3995.0
26	5878.0
27	6977.0
28	8845.0
29	10713.0
30	12503.0
31	14293.0
32	15838.0
33	17383.0
34	19810.5
35	22238.0
36	24267.5
37	26297.0
38	27994.5
39	29692.0
40	33232.5
41	36773.0
42	40368.5
43	43964.0
44	50043.0
45	56122.0
46	62607.5
47	69093.0
48	77453.0
49	85813.0
50	83261.5
51	80710.0
52	75069.0
53	69428.0
54	64962.0
55	60496.0
56	57930.5
57	55365.0
58	54891.5
59	54418.0
60	52916.5
61	51415.0
62	48307.0
63	45199.0
64	40386.5
65	35574.0
66	29349.0
67	23124.0
68	20331.5
69	17539.0
70	15130.5
71	12722.0
72	11133.0
73	9544.0
74	7773.5
75	4915.5
76	3828.0
77	2898.5
78	1969.0
79	1530.0
80	1091.0
81	857.5
82	624.0
83	423.5
84	223.0
85	200.5
86	178.0
87	121.0
88	64.0
89	58.5
90	53.0
91	33.5
92	14.0
93	17.5
94	21.0
95	12.0
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	977950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.07536829879983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0880213007706	20.23897747950862
2	9.21969840962598	5.17692856908217
3	3.313449709624276	2.790789628117587
4	1.7580508712730099	1.9743170279606277
5	1.1764728235116717	1.651495390680956
6	0.9553215673434663	1.609260290817271
7	0.7994292941282652	1.5710990303050436
8	0.6838582019759722	1.5359656707704372
9	0.604723160955415	1.528004291637392
>10	9.188658251059733	55.23343255983549
>50	0.14825543859356144	2.6372701920087716
>100	0.060034281523665196	2.9587448245436097
>500	0.002928501537739425	0.5695704546048685
>1k	0.0010981880766522845	0.5241445901271796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.21729127255994685	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1563	0.15982412188762204	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1412	0.1443836596963035	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	995	0.10174344291630452	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0450943299759702E-4	0.0	0.0	0.1163658673756327	0.0
2	2.0450943299759702E-4	0.0	0.0	0.589191676466077	0.0
3	2.0450943299759702E-4	0.0	0.0	0.9000460146224245	0.0
4	2.0450943299759702E-4	0.0	0.0	1.1923922490924894	0.0
5	2.0450943299759702E-4	0.0	0.0	2.0300628866506467	0.0
6	2.0450943299759702E-4	0.0	0.0	2.6789713175520222	0.0
7	2.0450943299759702E-4	0.0	0.0	3.2639705506416483	0.0
8	2.0450943299759702E-4	0.0	0.0	4.186614857610308	0.0
9	2.0450943299759702E-4	0.0	0.0	4.5762053274707295	0.0
10	2.0450943299759702E-4	0.0	0.0	5.32849327675239	0.0
11	2.0450943299759702E-4	0.0	0.0	6.27659900812925	0.0
12	2.0450943299759702E-4	0.0	0.0	7.006902193363669	0.0
13	2.0450943299759702E-4	0.0	0.0	7.330538371082366	0.0
14	2.0450943299759702E-4	0.0	0.0	7.465821361010277	0.0
15	2.0450943299759702E-4	0.0	0.0	7.716549925865331	0.0
16	2.0450943299759702E-4	0.0	0.0	8.205634234879083	0.0
17	2.0450943299759702E-4	0.0	0.0	8.779998977452834	0.0
18	2.0450943299759702E-4	0.0	0.0	9.442711795081548	0.0
19	2.0450943299759702E-4	0.0	0.0	9.807556623549262	0.0
20	2.0450943299759702E-4	0.0	0.0	10.184262999130835	0.0
21	2.0450943299759702E-4	0.0	0.0	10.681220921314996	0.0
22	2.0450943299759702E-4	0.0	0.0	11.1743954189887	0.0
23	2.0450943299759702E-4	0.0	0.0	11.687611841096171	0.0
24	2.0450943299759702E-4	0.0	0.0	12.093051792013906	0.0
25	2.0450943299759702E-4	0.0	0.0	12.458407894064115	0.0
26	2.0450943299759702E-4	0.0	0.0	12.791042486834705	0.0
27	2.0450943299759702E-4	0.0	0.0	13.151899381358966	0.0
28	2.0450943299759702E-4	0.0	0.0	13.519300577739148	0.0
29	2.0450943299759702E-4	0.0	0.0	13.899790377831177	0.0
30	2.0450943299759702E-4	0.0	0.0	14.371593639756634	0.0
31	2.0450943299759702E-4	0.0	0.0	14.753412751163147	0.0
32	2.0450943299759702E-4	0.0	0.0	15.14555958893604	0.0
33	2.0450943299759702E-4	0.0	0.0	15.53443427578097	0.0
34	3.067641494963955E-4	0.0	0.0	15.893757349557749	0.0
35	3.067641494963955E-4	0.0	0.0	16.299095045758985	0.0
36	3.067641494963955E-4	0.0	0.0	16.682856996778977	0.0
37	3.067641494963955E-4	0.0	0.0	17.06620992893297	0.0
38	3.067641494963955E-4	0.0	0.0	17.450892172401453	0.0
39	3.067641494963955E-4	0.0	0.0	17.851730661076743	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	40	6.8139343E-9	45.0	1
CCCGTAG	20	7.03277E-4	45.0	27
GTTAACG	20	7.03277E-4	45.0	1
CGCAATA	20	7.03277E-4	45.0	32
CGGTCTA	155	0.0	42.09677	31
GCGTAAG	95	0.0	40.26316	1
AATAGCG	40	3.457517E-7	39.375	1
TCACGAC	175	0.0	38.571426	25
TATAGCG	65	9.094947E-12	38.07692	1
ATAACGG	65	9.094947E-12	38.07692	2
ATAGGGT	220	0.0	37.840908	4
CTCACGA	185	0.0	37.7027	24
CGTAAGG	150	0.0	37.500004	2
GGATCGA	30	1.1397974E-4	37.499996	8
CGCGAGG	120	0.0	37.499996	2
ATCGACG	30	1.1397974E-4	37.499996	15
CCCGAAT	30	1.1397974E-4	37.499996	29
CGTTAGG	195	0.0	36.923077	2
TACGGGA	265	0.0	36.509434	4
TAAGGGA	715	0.0	36.503498	4
>>END_MODULE