SRR3549903_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801517
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3600	0.449148302531325	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	3372	0.4207022433710077	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	3248	0.40523157961715095	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	3111	0.38813899143748665	No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3025	0.3774093375436828	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	2023	0.25239639333913066	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1143	0.14260458605369566	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.131750168742522	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	891	0.11116420487650293	No Hit
ACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	847	0.10567461451223119	No Hit
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	829	0.10342887299957457	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGA	20	7.0320064E-4	45.000004	32
ACGATAG	30	2.1646756E-6	45.000004	1
ATATACG	20	7.0320064E-4	45.000004	1
CGTAGAA	20	7.0320064E-4	45.000004	31
TACGACA	20	7.0320064E-4	45.000004	20
TTACGAA	20	7.0320064E-4	45.000004	33
ATTGCGT	20	7.0320064E-4	45.000004	1
ATACCTC	20	7.0320064E-4	45.000004	22
GCGTATG	20	7.0320064E-4	45.000004	1
CCGCATG	20	7.0320064E-4	45.000004	30
TCGTTAG	35	1.2113196E-7	45.0	1
TAAACGG	50	2.1827873E-11	45.0	2
GATATGT	25	3.889745E-5	45.0	9
CGATATG	25	3.889745E-5	45.0	10
TGTCGAT	25	3.889745E-5	45.0	11
TACGGGC	190	0.0	43.81579	4
ACGGGTA	90	0.0	42.5	5
GCGTACG	80	0.0	42.187504	1
CACGACG	65	0.0	41.53846	26
CGGTCTA	65	0.0	41.53846	31