##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801517
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52933000797238	33.0	31.0	34.0	31.0	34.0
2	32.7012864355965	34.0	31.0	34.0	31.0	34.0
3	32.71304039714691	34.0	31.0	34.0	31.0	34.0
4	36.13983982872478	37.0	35.0	37.0	35.0	37.0
5	36.15025383117264	37.0	35.0	37.0	35.0	37.0
6	36.13540074633476	37.0	36.0	37.0	35.0	37.0
7	36.23407987603507	37.0	37.0	37.0	35.0	37.0
8	36.20035507668583	37.0	37.0	37.0	35.0	37.0
9	37.5790794206486	39.0	38.0	39.0	35.0	39.0
10	37.653841403239106	39.0	37.0	39.0	35.0	39.0
11	37.62222760091177	39.0	37.0	39.0	35.0	39.0
12	37.50066561283167	39.0	37.0	39.0	35.0	39.0
13	37.43553037552541	39.0	37.0	39.0	35.0	39.0
14	38.6987961577858	40.0	38.0	41.0	35.0	41.0
15	38.72728338887385	40.0	38.0	41.0	35.0	41.0
16	38.753516145010025	40.0	38.0	41.0	35.0	41.0
17	38.692011523149226	40.0	38.0	41.0	35.0	41.0
18	38.65079343295276	40.0	38.0	41.0	35.0	41.0
19	38.633085761125464	40.0	38.0	41.0	35.0	41.0
20	38.60130602345303	40.0	38.0	41.0	35.0	41.0
21	38.58929879216536	40.0	38.0	41.0	35.0	41.0
22	38.55883905144869	40.0	38.0	41.0	35.0	41.0
23	38.52917405370067	40.0	38.0	41.0	35.0	41.0
24	38.508551908443614	40.0	38.0	41.0	35.0	41.0
25	38.43105012120766	40.0	37.0	41.0	35.0	41.0
26	38.3482359076601	40.0	37.0	41.0	34.0	41.0
27	38.28001651867646	40.0	37.0	41.0	34.0	41.0
28	38.191305986024005	40.0	37.0	41.0	34.0	41.0
29	38.132538673540296	40.0	37.0	41.0	34.0	41.0
30	38.026769238830866	40.0	37.0	41.0	34.0	41.0
31	37.932547906033186	40.0	37.0	41.0	34.0	41.0
32	37.822643811672116	40.0	36.0	41.0	34.0	41.0
33	37.74861419034156	40.0	36.0	41.0	33.0	41.0
34	37.633642205966936	40.0	36.0	41.0	33.0	41.0
35	37.56846704436712	40.0	36.0	41.0	33.0	41.0
36	37.49956519949047	40.0	36.0	41.0	33.0	41.0
37	37.34971185888759	40.0	35.0	41.0	33.0	41.0
38	37.25403827991172	40.0	35.0	41.0	33.0	41.0
39	37.158002887025475	39.0	35.0	41.0	32.0	41.0
40	37.00273855701127	39.0	35.0	41.0	32.0	41.0
41	36.9518675212129	39.0	35.0	41.0	32.0	41.0
42	36.824178401705765	39.0	35.0	41.0	31.0	41.0
43	36.780677140971434	39.0	35.0	41.0	31.0	41.0
44	36.685764618841525	39.0	35.0	41.0	31.0	41.0
45	36.57014386469657	39.0	35.0	41.0	31.0	41.0
46	36.41155458960945	39.0	35.0	41.0	31.0	41.0
47	36.320836613571515	38.0	35.0	40.0	31.0	41.0
48	36.23548595974883	38.0	35.0	40.0	30.0	41.0
49	36.179512100180034	38.0	35.0	40.0	30.0	41.0
50	36.079107492417506	38.0	35.0	40.0	30.0	41.0
51	34.38525570886207	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	10.0
9	36.0
10	33.0
11	32.0
12	17.0
13	15.0
14	18.0
15	25.0
16	60.0
17	102.0
18	189.0
19	307.0
20	546.0
21	903.0
22	1320.0
23	1969.0
24	2789.0
25	3771.0
26	4573.0
27	5445.0
28	6394.0
29	7728.0
30	9617.0
31	12039.0
32	15910.0
33	22694.0
34	44188.0
35	62520.0
36	54864.0
37	86382.0
38	165474.0
39	291411.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51463162977204	21.201671330739085	24.695795597598057	11.587901441890814
2	29.945465910267654	26.551027613887168	25.421544396438257	18.081962079406924
3	31.47306919254364	25.504137778737068	26.26232506609342	16.76046796262587
4	28.558221472532708	26.018911638804916	26.19782237931323	19.225044509349146
5	26.25508878788597	30.1283690801318	23.45726915336793	20.159272978614304
6	25.49964629571176	35.91814022659532	23.54772263096104	15.034490846731885
7	79.05122411626952	6.737474064804615	9.072296657463285	5.139005161462577
8	79.0384982476978	6.5957428226725066	8.33032861436501	6.03543031526468
9	71.89005348607702	8.326211421591806	10.623230698787424	9.160504393543743
10	39.46279367748906	26.28815109348897	17.72064722270395	16.528408006318017
11	29.987261655086545	24.489935958937863	23.811347731863453	21.71145465411214
12	26.679908224030186	21.708585095512632	30.45412636288438	21.1573803175728
13	24.53322886476519	23.59351080513576	31.860709130311648	20.012551199787403
14	20.10861903116216	28.73613410570206	28.854035535116534	22.301211328019242
15	19.955160027797287	24.501289429918515	34.23370932868548	21.309841213598713
16	20.86568344776218	24.39835961058842	30.604341517397636	24.131615424251763
17	20.588958188035935	24.585255209808402	28.18555314484908	26.640233457306582
18	21.781696458091343	25.189983493799883	29.75619980611765	23.272120241991125
19	23.538739664910413	26.132321585194074	26.906852880225873	23.42208586966964
20	24.10142267724827	25.936068729671362	27.13454611692578	22.827962476154593
21	23.353590753533613	26.80067921204416	28.027477895041525	21.8182521393807
22	21.378710619986848	24.761670681969317	26.259330744076543	27.60028795396729
23	21.18744830115893	26.20942537712862	27.35363067782717	25.249495643885282
24	22.674004419120244	24.09481021612767	27.896102016551115	25.335083348200975
25	22.14301131479432	27.21639091872038	25.521105603499368	25.11949216298594
26	20.704613875937753	26.345292738644343	27.165113154181388	25.784980231236514
27	21.89123873854204	26.99381298213263	26.853329374174223	24.26161890515111
28	19.524476711036698	26.62139418128374	28.982541855007444	24.87158725267212
29	21.35687702194713	25.897641597121456	27.450946143375628	25.294535237555788
30	21.79828999260153	26.807166909747394	26.651087874617758	24.743455223033322
31	23.27324311274745	26.98894720885521	22.847051279012174	26.890758399385167
32	21.886372965264616	29.732494756817385	23.74010782054529	24.64102445737271
33	21.04322179067942	27.04796030527113	22.93725522977055	28.9715626742789
34	19.88978399709551	25.01431660214319	26.639110586550252	28.456788814211052
35	19.632521830478954	27.011654150816515	25.920722829334874	27.43510118936966
36	20.98508203818509	26.791696245993535	24.986993413739196	27.23622830208218
37	19.92858541989752	29.189399601006592	25.563899455657207	25.318115523438678
38	19.839753866730213	29.08098019131222	23.44791189706519	27.631354044892376
39	21.210529533372345	25.27731788595875	25.288172303269924	28.22398027739898
40	21.38033254441266	24.550321452944853	28.25829021717568	25.811055785466806
41	18.811952834437697	25.607192361484536	26.295512135113793	29.285342668963978
42	19.896770748468217	26.220778848109273	26.989446262524687	26.893004140897826
43	21.869405140502323	23.94035310542384	26.74129182537613	27.448949928697708
44	20.676916397281655	24.043407688171307	26.975971813448744	28.303704101098294
45	21.095622425974746	23.779657823851522	26.102004074773212	29.022715675400523
46	22.72902508618033	24.7042795099792	26.576604114447978	25.990091289392492
47	19.84823777911136	24.47920630504406	29.19201963277136	26.480536283073224
48	19.700018839276023	25.03926928561715	27.465293936373154	27.795417938733674
49	20.343548546069513	23.193144998796033	29.940225846738123	26.523080608396327
50	20.402561642485438	22.677996848476077	28.706440412368046	28.213001096670435
51	20.14018417575672	22.74823865245528	26.179107866707756	30.93246930508024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	506.0
1	901.0
2	1296.0
3	1779.5
4	2263.0
5	1774.0
6	1285.0
7	1159.0
8	1033.0
9	1125.0
10	1217.0
11	1273.5
12	1330.0
13	1363.0
14	1396.0
15	1428.5
16	1461.0
17	1523.5
18	1586.0
19	1548.0
20	1510.0
21	1763.0
22	2016.0
23	1992.0
24	1968.0
25	3117.0
26	4881.5
27	5497.0
28	6974.0
29	8451.0
30	9842.5
31	11234.0
32	12189.5
33	13145.0
34	15293.0
35	17441.0
36	18008.5
37	18576.0
38	20388.0
39	22200.0
40	26850.5
41	31501.0
42	35752.5
43	40004.0
44	47110.0
45	54216.0
46	67945.5
47	81675.0
48	85574.5
49	89474.0
50	86243.5
51	83013.0
52	69861.5
53	56710.0
54	50188.5
55	43667.0
56	39953.5
57	36240.0
58	34072.5
59	31905.0
60	31226.0
61	30547.0
62	28832.0
63	27117.0
64	25216.0
65	23315.0
66	20132.5
67	16950.0
68	14128.5
69	11307.0
70	9899.5
71	8492.0
72	7618.5
73	6745.0
74	5345.0
75	3077.5
76	2210.0
77	1768.0
78	1326.0
79	1041.0
80	756.0
81	557.5
82	359.0
83	271.5
84	184.0
85	144.5
86	105.0
87	70.5
88	36.0
89	28.0
90	20.0
91	12.5
92	5.0
93	5.0
94	5.0
95	6.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	801517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.790025854546474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71210184577737	19.650911385234767
2	11.678270824434122	6.490788962948402
3	4.550011693461269	3.7933482779933234
4	2.2427382673254406	2.4930301773581904
5	1.3543733516576957	1.8819035229638064
6	0.9353217503990848	1.5595569375546137
7	0.6417511597782025	1.2483996925695005
8	0.5224760547962707	1.1615698456935841
9	0.41417081013512397	1.03588357696682
>10	6.29032054700376	43.77768889297993
>50	0.5769439080921481	9.626980759886141
>100	0.07116091916048403	3.514431232365123
>500	0.006755783464602913	1.1899176511305682
>1k	0.0036030845144548874	2.5755890843551943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3600	0.449148302531325	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	3372	0.4207022433710077	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	3248	0.40523157961715095	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	3111	0.38813899143748665	No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3025	0.3774093375436828	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	2023	0.25239639333913066	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1143	0.14260458605369566	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.131750168742522	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	891	0.11116420487650293	No Hit
ACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	847	0.10567461451223119	No Hit
CGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTG	829	0.10342887299957457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2716099596140818	0.0
2	0.0	0.0	0.0	1.4396450730302663	0.0
3	0.0	0.0	0.0	1.975503950633611	0.0
4	0.0	0.0	0.0	2.6392453310410136	0.0
5	0.0	0.0	0.0	4.653800231311376	0.0
6	0.0	0.0	0.0	5.627828230717502	0.0
7	0.0	0.0	0.0	6.6980488249157535	0.0
8	0.0	0.0	0.0	8.311115048090059	0.0
9	0.0	0.0	0.0	8.932062576339616	0.0
10	0.0	0.0	0.0	10.620984957274768	0.0
11	1.247634173698125E-4	0.0	0.0	12.307412069862524	0.0
12	1.247634173698125E-4	0.0	0.0	14.085166003964982	0.0
13	1.247634173698125E-4	0.0	0.0	14.651092865154451	0.0
14	1.247634173698125E-4	0.0	0.0	14.893383421686627	0.0
15	1.247634173698125E-4	0.0	0.0	15.34402888522639	0.0
16	1.247634173698125E-4	0.0	0.0	16.232219653482083	0.0
17	1.247634173698125E-4	0.0	0.0	17.268130307903636	0.0
18	1.247634173698125E-4	0.0	0.0	18.49230895913624	0.0
19	1.247634173698125E-4	0.0	0.0	19.14357399780666	0.0
20	1.247634173698125E-4	0.0	0.0	19.758033828352985	0.0
21	1.247634173698125E-4	0.0	0.0	20.509608654588735	0.0
22	1.247634173698125E-4	0.0	0.0	21.302105881721786	0.0
23	1.247634173698125E-4	0.0	0.0	22.0587960080697	0.0
24	1.247634173698125E-4	0.0	0.0	22.72952413984981	0.0
25	1.247634173698125E-4	0.0	0.0	23.259893427088883	0.0
26	1.247634173698125E-4	0.0	0.0	23.761691891750267	0.0
27	1.247634173698125E-4	0.0	0.0	24.27259808587965	0.0
28	1.247634173698125E-4	0.0	0.0	24.79872541692815	0.0
29	1.247634173698125E-4	0.0	0.0	25.34968066803324	0.0
30	1.247634173698125E-4	0.0	0.0	25.985974096619287	0.0
31	1.247634173698125E-4	0.0	0.0	26.531190230525365	0.0
32	1.247634173698125E-4	0.0	0.0	27.06018712017337	0.0
33	1.247634173698125E-4	0.0	0.0	27.583195365787624	0.0
34	1.247634173698125E-4	0.0	0.0	28.090233893978542	0.0
35	1.247634173698125E-4	0.0	0.0	28.634701447380404	0.0
36	1.247634173698125E-4	0.0	0.0	29.135002751033355	0.0
37	1.247634173698125E-4	0.0	0.0	29.60935326387338	0.0
38	1.247634173698125E-4	0.0	0.0	30.10740882601367	0.0
39	1.247634173698125E-4	0.0	0.0	30.592239465912762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGA	20	7.0320064E-4	45.000004	32
ACGATAG	30	2.1646756E-6	45.000004	1
ATATACG	20	7.0320064E-4	45.000004	1
CGTAGAA	20	7.0320064E-4	45.000004	31
TACGACA	20	7.0320064E-4	45.000004	20
TTACGAA	20	7.0320064E-4	45.000004	33
ATTGCGT	20	7.0320064E-4	45.000004	1
ATACCTC	20	7.0320064E-4	45.000004	22
GCGTATG	20	7.0320064E-4	45.000004	1
CCGCATG	20	7.0320064E-4	45.000004	30
TCGTTAG	35	1.2113196E-7	45.0	1
TAAACGG	50	2.1827873E-11	45.0	2
GATATGT	25	3.889745E-5	45.0	9
CGATATG	25	3.889745E-5	45.0	10
TGTCGAT	25	3.889745E-5	45.0	11
TACGGGC	190	0.0	43.81579	4
ACGGGTA	90	0.0	42.5	5
GCGTACG	80	0.0	42.187504	1
CACGACG	65	0.0	41.53846	26
CGGTCTA	65	0.0	41.53846	31
>>END_MODULE