##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571569
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44751027435008	33.0	31.0	34.0	31.0	34.0
2	32.62852778929578	34.0	31.0	34.0	31.0	34.0
3	32.62838432455224	34.0	31.0	34.0	31.0	34.0
4	36.07711579879245	37.0	35.0	37.0	35.0	37.0
5	36.093636988710024	37.0	35.0	37.0	35.0	37.0
6	36.08601761117205	37.0	35.0	37.0	35.0	37.0
7	36.212840444460774	37.0	36.0	37.0	35.0	37.0
8	36.192851606717646	37.0	37.0	37.0	35.0	37.0
9	37.54815079194288	39.0	38.0	39.0	35.0	39.0
10	37.60004303942306	39.0	37.0	39.0	35.0	39.0
11	37.56119383661465	39.0	37.0	39.0	35.0	39.0
12	37.37164891727858	39.0	37.0	39.0	35.0	39.0
13	37.26644377144317	39.0	37.0	39.0	34.0	39.0
14	38.45152553759913	40.0	38.0	41.0	34.0	41.0
15	38.49686914440776	40.0	38.0	41.0	35.0	41.0
16	38.52730991358874	40.0	38.0	41.0	35.0	41.0
17	38.452645262426756	40.0	37.0	41.0	34.0	41.0
18	38.39484121777073	40.0	37.0	41.0	34.0	41.0
19	38.37450596515906	40.0	37.0	41.0	34.0	41.0
20	38.35791829157984	40.0	37.0	41.0	34.0	41.0
21	38.32497738680719	40.0	37.0	41.0	34.0	41.0
22	38.3011429941092	40.0	37.0	41.0	34.0	41.0
23	38.27846856634982	40.0	37.0	41.0	34.0	41.0
24	38.258047584806036	40.0	37.0	41.0	34.0	41.0
25	38.16036209101613	40.0	36.0	41.0	34.0	41.0
26	38.112898005315195	40.0	36.0	41.0	34.0	41.0
27	38.019010828088994	40.0	36.0	41.0	34.0	41.0
28	37.894593653609626	40.0	36.0	41.0	34.0	41.0
29	37.81975404544333	40.0	36.0	41.0	34.0	41.0
30	37.726536953543665	40.0	36.0	41.0	33.0	41.0
31	37.55233401391608	40.0	35.0	41.0	33.0	41.0
32	37.45459953216497	40.0	35.0	41.0	33.0	41.0
33	37.34516042682511	40.0	35.0	41.0	33.0	41.0
34	37.16789748919203	40.0	35.0	41.0	32.0	41.0
35	37.131030549242524	40.0	35.0	41.0	32.0	41.0
36	37.037607008077764	39.0	35.0	41.0	32.0	41.0
37	36.86365425696635	39.0	35.0	41.0	31.0	41.0
38	36.73197986594794	39.0	35.0	41.0	31.0	41.0
39	36.709870549312505	39.0	35.0	41.0	31.0	41.0
40	36.58277478309705	39.0	35.0	41.0	31.0	41.0
41	36.51097942680586	39.0	35.0	41.0	31.0	41.0
42	36.343073189763615	39.0	35.0	41.0	31.0	41.0
43	36.24195853868912	39.0	35.0	40.0	30.0	41.0
44	36.12020420981544	38.0	35.0	40.0	30.0	41.0
45	36.02684890188236	38.0	35.0	40.0	30.0	41.0
46	35.89874888246214	38.0	35.0	40.0	30.0	41.0
47	35.80972900909602	38.0	35.0	40.0	29.0	41.0
48	35.67005908298036	38.0	35.0	40.0	29.0	41.0
49	35.58588201949371	38.0	35.0	40.0	29.0	41.0
50	35.43227676798427	37.0	34.0	40.0	28.0	41.0
51	33.70996852523492	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	18.0
10	29.0
11	27.0
12	21.0
13	13.0
14	24.0
15	33.0
16	33.0
17	85.0
18	166.0
19	292.0
20	506.0
21	781.0
22	1166.0
23	1751.0
24	2547.0
25	3417.0
26	4112.0
27	4704.0
28	5338.0
29	6365.0
30	7882.0
31	9753.0
32	12870.0
33	18614.0
34	37907.0
35	50139.0
36	39716.0
37	62087.0
38	118438.0
39	182650.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46066529150461	22.051405867008185	23.794152587001744	11.69377625448546
2	31.846723667658672	25.45711891302712	24.75204218563288	17.944115233681323
3	30.3524158937941	25.798634985452324	26.49041498051854	17.358534140235037
4	28.200269783700655	26.29026416758082	25.792686447305574	19.716779601412952
5	25.384336799231587	30.157863704994497	24.503603239503892	19.95419625627002
6	25.365091528756807	34.76588128467429	23.691277868463825	16.177749318105075
7	78.73047698528086	6.653964788153312	9.198189544919336	5.417368681646486
8	78.01402805260608	6.378057592346681	8.894464185426433	6.713450169620816
9	71.5938058222192	8.24852292549106	11.9968017859611	8.160869466328649
10	39.41326419032522	25.019551445232334	18.68715763101218	16.88002673343026
11	31.13709805815221	23.914348048966964	24.58723268756703	20.361321205313796
12	27.828311192524435	21.150902165792758	30.529997253175033	20.490789388507775
13	24.63727039080146	24.299078501458265	30.88743441299301	20.176216694747264
14	19.195582685555024	29.045662028556485	29.132790616705943	22.62596466918255
15	18.23804300093252	25.144645703318407	35.89382909150076	20.723482204248306
16	20.391588767060494	25.04457029684955	31.206555988865738	23.35728494722422
17	20.459821998743806	24.3534901298006	28.37137773392189	26.8153101375337
18	21.855279065169732	24.551716415690844	30.55361644875772	23.0393880703817
19	23.015069046781754	26.434253782133037	27.354702581840513	23.195974589244695
20	24.107150667723406	25.972017376729667	27.92681198595445	21.994019969592475
21	23.06388205098597	27.42660991061447	28.23298674350778	21.276521294891783
22	20.695838997566348	25.959245515414587	26.781893349709307	26.563022137309755
23	21.089317300273457	26.55217480304215	27.551529211696224	24.806978684988167
24	23.029240564131364	24.17450911438514	28.288448113876015	24.50780220760748
25	21.020034326564247	27.101714753599303	26.57421938558599	25.30403153425046
26	20.731880140455484	26.468895268987648	27.541906576458835	25.257318014098036
27	23.316170051209912	26.311433965103078	27.076695901982088	23.295700081704922
28	20.71998306416198	27.18954316976603	28.20044474070497	23.890029025367017
29	22.84081187048283	26.752850486992823	25.777115273921435	24.629222368602914
30	25.281462080693668	25.36544144276544	25.794610974353056	23.558485502187835
31	24.26548675662956	28.262379520232905	24.06236167461846	23.409772048519077
32	24.881860282835493	30.031544747878208	22.48634897973823	22.600245989548068
33	25.06923923445813	26.335927945707343	23.47748040918944	25.117352410645083
34	21.892369950084767	26.35622295820802	28.03073644651827	23.720670645188946
35	23.69652657859331	26.58086775175001	25.06748966441497	24.655116005241712
36	25.967468494617446	27.425035297575622	24.035243338949453	22.57225286885748
37	23.095899182775835	28.98705143211056	27.444105611046087	20.472943774067524
38	21.447104374100064	31.110329636491834	24.243792088094352	23.19877390131375
39	23.899126789591456	25.982339839984327	25.924429071555664	24.194104298868552
40	24.512176132715386	23.754787261030604	28.601971065610627	23.131065540643387
41	21.756953228744035	25.88471383157589	26.550425232998993	25.807907706681082
42	24.858765958265757	25.37786339007189	26.548325748947192	23.215044902715157
43	25.30035743715982	24.192179771821078	26.36654542146268	24.14091736955643
44	22.479175742561267	25.09880696818757	25.976041387828943	26.445975901422226
45	22.77187881078225	24.09455376341264	25.878240422416194	27.255327003388917
46	23.964385752201398	24.746268604490446	27.411563608243274	23.877782035064882
47	21.29769109241404	24.69658081526465	30.16818616824915	23.83754192407216
48	21.67559822173701	24.282282629043912	28.815593567880693	25.22652558133839
49	23.696351621588995	22.49089786185045	29.81704746058656	23.995703055973994
50	21.44413010502669	22.366153517773007	30.081757408116953	26.10795896908335
51	21.17557110340134	22.352856785444978	26.73273043149646	29.738841679657224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	594.0
1	906.0
2	1218.0
3	1506.0
4	1794.0
5	1411.0
6	1028.0
7	991.5
8	955.0
9	996.0
10	1037.0
11	1131.0
12	1225.0
13	1271.5
14	1318.0
15	1317.0
16	1316.0
17	1267.5
18	1219.0
19	1410.5
20	1602.0
21	1588.5
22	1575.0
23	1705.0
24	1835.0
25	2460.0
26	3873.0
27	4661.0
28	5167.5
29	5674.0
30	6570.0
31	7466.0
32	8184.5
33	8903.0
34	10256.0
35	11609.0
36	12205.5
37	12802.0
38	13916.5
39	15031.0
40	17723.0
41	20415.0
42	24431.5
43	28448.0
44	33675.5
45	38903.0
46	47271.0
47	55639.0
48	61110.5
49	66582.0
50	64994.5
51	63407.0
52	53996.0
53	44585.0
54	38383.0
55	32181.0
56	29186.5
57	26192.0
58	24010.0
59	21828.0
60	21664.0
61	21500.0
62	20170.5
63	18841.0
64	16734.0
65	14627.0
66	12811.0
67	10995.0
68	8663.5
69	6332.0
70	5901.0
71	5470.0
72	4664.5
73	3859.0
74	3256.0
75	2026.0
76	1399.0
77	1146.0
78	893.0
79	684.5
80	476.0
81	331.5
82	187.0
83	141.0
84	95.0
85	79.5
86	64.0
87	41.0
88	18.0
89	15.0
90	12.0
91	7.5
92	3.0
93	9.0
94	15.0
95	9.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	571569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.181481483711252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.58182345932563	21.30263997824213
2	12.059963352655142	7.279751212447949
3	4.744077202396477	4.29549834724278
4	2.394626533030984	2.8909350546831325
5	1.3754411281118215	2.075642547002093
6	0.9189453558892259	1.664107934598787
7	0.641764654661046	1.3558585629086894
8	0.48253668538147515	1.1650937628041917
9	0.4055585918667607	1.1016323217887982
>10	5.93198723439929	43.40999082624161
>50	0.38849736256953393	7.174726429012514
>100	0.06659954786906287	3.6463429886701877
>500	0.005842065602549375	1.1598473974599854
>1k	0.00233682624101975	1.477932636897172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2848	0.49827754829250714	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2750	0.4811317618695206	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	1758	0.3075744135878608	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1026	0.17950588642841023	No Hit
ACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	898	0.15711138987593798	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.131217753237142	No Hit
AGCCGAGGATTGATATTTATTTTTGCATTGCAATAGCTGAAGGCGTTTTAT	735	0.12859339817239912	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	681	0.1191457199393249	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	645	0.1128472677839421	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	642	0.11232239677099352	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	638	0.11162256875372878	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33311813621802444	0.0
2	0.0	0.0	0.0	1.756043452321592	0.0
3	0.0	0.0	0.0	2.4467737053619074	0.0
4	0.0	0.0	0.0	3.244052774030782	0.0
5	0.0	0.0	0.0	5.309070295974764	0.0
6	0.0	0.0	0.0	6.462036954418451	0.0
7	0.0	0.0	0.0	7.622176850039103	0.0
8	0.0	0.0	0.0	9.527808541051037	0.0
9	0.0	0.0	0.0	10.263502744200613	0.0
10	0.0	0.0	0.0	11.853511999426141	0.0
11	0.0	0.0	0.0	13.86271823699326	0.0
12	0.0	0.0	0.0	15.557351780799868	0.0
13	0.0	0.0	0.0	16.20469269676977	0.0
14	0.0	0.0	0.0	16.461004708092986	0.0
15	0.0	0.0	0.0	16.944060997009984	0.0
16	0.0	0.0	0.0	17.917871683033894	0.0
17	0.0	0.0	0.0	19.09113335397826	0.0
18	0.0	0.0	0.0	20.597688118144966	0.0
19	0.0	0.0	0.0	21.340555558471504	0.0
20	0.0	0.0	0.0	22.10879176442389	0.0
21	0.0	0.0	0.0	22.980952430940096	0.0
22	0.0	0.0	0.0	23.825819804782974	0.0
23	0.0	0.0	0.0	24.640769530887784	0.0
24	0.0	0.0	0.0	25.324851417764084	0.0
25	0.0	0.0	0.0	25.94944792317288	0.0
26	0.0	0.0	0.0	26.522082198299767	0.0
27	0.0	0.0	0.0	27.08124478409431	0.0
28	0.0	0.0	0.0	27.609964851137832	0.0
29	0.0	0.0	0.0	28.212166859994156	0.0
30	0.0	0.0	0.0	28.963432236527872	0.0
31	0.0	0.0	0.0	29.56231006230219	0.0
32	1.749570043161893E-4	0.0	0.0	30.17325992137432	0.0
33	1.749570043161893E-4	0.0	0.0	30.762165197902615	0.0
34	1.749570043161893E-4	0.0	0.0	31.340748011176252	0.0
35	1.749570043161893E-4	0.0	0.0	31.916006641367883	0.0
36	1.749570043161893E-4	0.0	0.0	32.450675246558156	0.0
37	1.749570043161893E-4	0.0	0.0	33.019985338603036	0.0
38	1.749570043161893E-4	0.0	0.0	33.58422167752275	0.0
39	1.749570043161893E-4	0.0	0.0	34.16735337290861	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAAGC	20	7.030306E-4	45.000004	14
GACGTAG	25	3.8883354E-5	45.000004	1
AGCCACG	25	3.8883354E-5	45.000004	1
CGTCTAC	35	1.210501E-7	45.000004	25
TAAGTCG	20	7.030306E-4	45.000004	17
GGTCAAT	30	2.1635788E-6	45.000004	8
ACCTCGT	20	7.030306E-4	45.000004	24
TATTCGG	20	7.030306E-4	45.000004	42
AATTCGT	30	2.1635788E-6	45.000004	31
TTAACGG	25	3.8883354E-5	45.000004	2
ACTCGAA	20	7.030306E-4	45.000004	45
CGGTCTA	20	7.030306E-4	45.000004	31
ACCGTAG	20	7.030306E-4	45.000004	17
CACACCG	35	1.210501E-7	45.000004	17
CGACTCA	20	7.030306E-4	45.000004	34
AGTAGAT	20	7.030306E-4	45.000004	19
CGTCAGT	20	7.030306E-4	45.000004	15
TGCACTA	20	7.030306E-4	45.000004	41
ATCGACG	25	3.8883354E-5	45.000004	1
TGCAACG	20	7.030306E-4	45.000004	1
>>END_MODULE