FastQCFastQC Report
Sat 18 Jun 2016
SRR3549899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467075
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC20080.4299095434352085No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC14180.30359150029438525No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT10210.2185944441470856No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.14151902799336294No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5450.11668361612160788No Hit
TCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC5440.11646951774340308No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTG4800.10276722153829684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT302.1627238E-645.0000044
AATGACG302.1627238E-645.0000041
CTTGTCG253.887237E-545.042
CTATGCG207.028982E-445.01
CGAACGA207.028982E-445.022
CGACGTC253.887237E-545.018
CGCGGAA207.028982E-445.015
GCGAAGT207.028982E-445.018
GACGTCG253.887237E-545.019
TAACGCG207.028982E-445.01
ATCGTAC253.887237E-545.028
TCGCTAT253.887237E-545.023
GTCGTGC207.028982E-445.024
CGAATCC207.028982E-445.023
AGCGAAC207.028982E-445.010
TCATCGC207.028982E-445.032
ATAACGG406.7993824E-945.02
TAAGCGA207.028982E-445.025
CCACGAC207.028982E-445.011
TGTCGGC253.887237E-545.044