##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467075
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55986083605417	34.0	31.0	34.0	31.0	34.0
2	32.742574532997914	34.0	31.0	34.0	31.0	34.0
3	32.74940213027886	34.0	31.0	34.0	31.0	34.0
4	36.156784242359365	37.0	37.0	37.0	35.0	37.0
5	36.17119734518011	37.0	35.0	37.0	35.0	37.0
6	36.16863886956056	37.0	36.0	37.0	35.0	37.0
7	36.26292993630573	37.0	37.0	37.0	35.0	37.0
8	36.265411336509125	37.0	37.0	37.0	35.0	37.0
9	37.65496547663651	39.0	38.0	39.0	35.0	39.0
10	37.703197559278486	39.0	37.0	39.0	35.0	39.0
11	37.64817641706364	39.0	37.0	39.0	35.0	39.0
12	37.45861371300112	39.0	37.0	39.0	35.0	39.0
13	37.357809773590965	39.0	37.0	39.0	35.0	39.0
14	38.60247711823583	40.0	38.0	41.0	35.0	41.0
15	38.66610715623829	40.0	38.0	41.0	35.0	41.0
16	38.69989402130279	40.0	38.0	41.0	35.0	41.0
17	38.65486485039876	40.0	38.0	41.0	35.0	41.0
18	38.5939410158968	40.0	38.0	41.0	35.0	41.0
19	38.55930632125462	40.0	37.0	41.0	35.0	41.0
20	38.51256436332495	40.0	37.0	41.0	35.0	41.0
21	38.490270299202486	40.0	37.0	41.0	35.0	41.0
22	38.4463201841246	40.0	37.0	41.0	35.0	41.0
23	38.4116041320987	40.0	37.0	41.0	35.0	41.0
24	38.39982872129744	40.0	37.0	41.0	35.0	41.0
25	38.3336787453835	40.0	37.0	41.0	34.0	41.0
26	38.240802868918266	40.0	37.0	41.0	34.0	41.0
27	38.14794840229085	40.0	36.0	41.0	34.0	41.0
28	38.0296483434138	40.0	36.0	41.0	34.0	41.0
29	37.9762307980517	40.0	36.0	41.0	34.0	41.0
30	37.88961515816518	40.0	36.0	41.0	34.0	41.0
31	37.73213937804421	40.0	36.0	41.0	33.0	41.0
32	37.624049670823744	40.0	36.0	41.0	33.0	41.0
33	37.527874538350375	40.0	35.0	41.0	33.0	41.0
34	37.39656158004603	40.0	35.0	41.0	33.0	41.0
35	37.350269228710594	40.0	35.0	41.0	33.0	41.0
36	37.2030166461489	40.0	35.0	41.0	33.0	41.0
37	37.068695605630786	39.0	35.0	41.0	32.0	41.0
38	36.944691965958356	39.0	35.0	41.0	32.0	41.0
39	36.875306963549754	39.0	35.0	41.0	32.0	41.0
40	36.78455280201253	39.0	35.0	41.0	31.0	41.0
41	36.6925226141412	39.0	35.0	41.0	31.0	41.0
42	36.51159449767168	39.0	35.0	41.0	31.0	41.0
43	36.45677674891613	39.0	35.0	41.0	31.0	41.0
44	36.33751752930471	39.0	35.0	41.0	31.0	41.0
45	36.24977144998127	38.0	35.0	41.0	31.0	41.0
46	36.107389605523736	38.0	35.0	40.0	30.0	41.0
47	36.01517957501472	38.0	35.0	40.0	30.0	41.0
48	35.86288283466253	38.0	35.0	40.0	30.0	41.0
49	35.771822512444466	38.0	35.0	40.0	29.0	41.0
50	35.659491516351764	38.0	35.0	40.0	29.0	41.0
51	33.95603703901943	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	16.0
10	25.0
11	14.0
12	12.0
13	10.0
14	20.0
15	25.0
16	41.0
17	61.0
18	116.0
19	189.0
20	402.0
21	570.0
22	874.0
23	1279.0
24	1959.0
25	2409.0
26	2984.0
27	3553.0
28	4209.0
29	4910.0
30	5857.0
31	7300.0
32	9510.0
33	13730.0
34	28144.0
35	41573.0
36	31670.0
37	49679.0
38	93465.0
39	162359.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24241288872237	21.68324144944602	25.13022533854306	11.944120323288551
2	31.693625220788952	25.181823047690415	25.61258898463844	17.511962746882194
3	32.66070759513997	24.321789862441793	26.258523791682276	16.758978750735963
4	28.66991382540277	26.279077235989938	25.456939463683565	19.594069474923728
5	24.840550232831987	30.765294652893004	24.85232564363325	19.541829470641762
6	25.592677835465395	34.48525397420114	24.314938714339238	15.607129475994219
7	80.1792003425574	6.831236953380078	8.153080340416421	4.836482363646096
8	80.96815286624204	5.619440132740994	8.022480329711502	5.389926671305465
9	74.33024674838087	7.917572124391158	10.588663490874056	7.163517636353904
10	41.073917465075205	25.620296526253817	17.91361130439437	15.392174704276615
11	32.08563935128191	25.055933201306	22.702563828079004	20.155863619333083
12	27.986083605416688	22.380131670502596	29.892415564952096	19.74136915912862
13	25.054862709414977	25.673607022426804	29.8568752341701	19.41465503398812
14	20.023550821602527	28.94267515923567	28.720012845902694	22.31376117325911
15	19.275276989776803	24.715302681582187	34.38783921211797	21.621581116523043
16	21.17454370283145	24.628378740031046	30.67687202269443	23.520205534443075
17	22.100305090188943	23.266070759513998	27.379542899962534	27.25408125033453
18	22.45956216881657	24.921051223036986	29.7001552213242	22.919231386822243
19	24.98613713001124	25.144784028260986	26.352298881335972	23.5167799603918
20	24.67440989134507	25.728630305625433	26.88968581062998	22.707273992399507
21	24.60889578761441	26.491034630412674	27.884600974147624	21.015468607825298
22	23.009152705668253	24.258416742493175	26.173740833913183	26.55868971792539
23	21.579189637638496	26.581812342771507	26.494246106085743	25.344751913504254
24	22.448643151528128	25.250334528715946	28.274045924102126	24.026976395653804
25	21.956002783278915	25.860729004977788	26.987100572713164	25.196167639030136
26	21.609591607343575	27.04833270887973	26.89803564737997	24.444040036396725
27	23.73644489642991	26.439865118021732	26.58866349087406	23.2350264946743
28	20.97928598190869	25.00990204999197	29.353744045388858	24.657067922710485
29	22.74773858588021	24.262270513300862	28.013488197826902	24.976502702992025
30	24.652357758389982	24.89707220467805	26.99587860621956	23.454691430712412
31	26.30112936894503	25.756891291548467	24.64828988920409	23.293689450302413
32	25.641064068939677	25.843601134721407	24.547449553069637	23.967885243269283
33	24.59647808167853	25.6061660332923	25.186747310389123	24.610608574640047
34	22.98474549055291	24.97778729326125	27.767489161269605	24.269978054916233
35	23.886099662795054	24.462024300165925	26.15382968474014	25.498046352298882
36	26.10030509018894	26.3936198683295	26.1701011614837	21.33597387999786
37	23.981159342717977	26.82631269068137	27.510999304180274	21.681528662420384
38	21.425895198843868	28.982069260825348	26.052561151849275	23.53947438848151
39	22.723545469143072	25.916608681689233	27.649735053256975	23.710110795910722
40	25.23577583899802	24.367606915377614	27.942835733019322	22.45378151260504
41	22.299202483541187	25.63656800299738	26.410105443451265	25.65412407001017
42	22.653321201091902	26.341808060803938	26.530214633624148	24.474656104480008
43	23.76941604667345	23.757426537493977	27.53776160145587	24.935395814376708
44	22.27415297329123	23.379971096718943	29.097040089921318	25.248835840068512
45	23.211047476315365	23.70689932023765	27.067387464539955	26.014665738907027
46	24.36503773483916	24.689396777819407	28.111117058288283	22.83444842905315
47	22.905529090617136	24.782315473960285	29.06835090724188	23.243804528180696
48	22.0001070491891	24.857678103088368	28.55794037360167	24.58427447412086
49	22.79740940962372	23.21575764063587	30.254884119252797	23.73194883048761
50	21.822619493657335	23.1722956698603	29.870149333618794	25.134935502863563
51	21.762243751003584	23.41444093560991	26.858213349033882	27.965101964352616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	423.0
1	685.5
2	948.0
3	1242.5
4	1537.0
5	1217.5
6	898.0
7	803.5
8	709.0
9	803.5
10	898.0
11	900.0
12	902.0
13	927.5
14	953.0
15	1004.0
16	1055.0
17	1010.5
18	966.0
19	1078.0
20	1190.0
21	1271.0
22	1352.0
23	1462.5
24	1573.0
25	2038.0
26	2722.0
27	2941.0
28	3784.5
29	4628.0
30	5456.0
31	6284.0
32	7111.5
33	7939.0
34	8542.0
35	9145.0
36	10343.5
37	11542.0
38	12229.5
39	12917.0
40	15457.0
41	17997.0
42	21103.0
43	24209.0
44	28552.0
45	32895.0
46	37284.0
47	41673.0
48	45299.5
49	48926.0
50	47707.5
51	46489.0
52	40437.5
53	34386.0
54	30279.5
55	26173.0
56	24851.5
57	23530.0
58	22267.0
59	21004.0
60	19279.5
61	17555.0
62	16604.0
63	15653.0
64	14511.0
65	13369.0
66	11480.5
67	9592.0
68	8538.5
69	7485.0
70	6466.0
71	5447.0
72	4627.5
73	3808.0
74	3233.0
75	1914.0
76	1170.0
77	951.5
78	733.0
79	616.5
80	500.0
81	376.5
82	253.0
83	184.0
84	115.0
85	83.5
86	52.0
87	54.5
88	57.0
89	39.0
90	21.0
91	17.5
92	14.0
93	9.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	467075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.798440143646083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37049846724042	21.56527966741894
2	10.923095543867495	6.509824174945256
3	4.066772956599294	3.6355047157506806
4	1.9482680457938644	2.322213949854672
5	1.2659674149679692	1.8861927119364696
6	0.8124339781074494	1.4525559160379462
7	0.6176688067224008	1.2883896876000331
8	0.5249290036520176	1.2513652395990738
9	0.4201539393326596	1.1267938811089229
>10	6.644538838652196	48.586485745826856
>50	0.34503860573078476	6.1422953792625385
>100	0.056303370809625955	2.900145979779969
>500	0.002165514261908691	0.3764188581632386
>1k	0.002165514261908691	0.956534092715384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	2008	0.4299095434352085	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	1418	0.30359150029438525	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	1021	0.2185944441470856	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.14151902799336294	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	545	0.11668361612160788	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	544	0.11646951774340308	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTG	480	0.10276722153829684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2382914949419258	0.0
2	0.0	0.0	0.0	1.2914414173312636	0.0
3	0.0	0.0	0.0	1.780228014772788	0.0
4	0.0	0.0	0.0	2.2855001873360807	0.0
5	0.0	0.0	0.0	3.720173419686346	0.0
6	0.0	0.0	0.0	4.569073489268319	0.0
7	0.0	0.0	0.0	5.452229299363057	0.0
8	0.0	0.0	0.0	6.86485039875823	0.0
9	0.0	0.0	0.0	7.468393726917519	0.0
10	0.0	0.0	0.0	8.61660332922978	0.0
11	0.0	0.0	0.0	10.133062142054275	0.0
12	0.0	0.0	0.0	11.403307819943263	0.0
13	0.0	0.0	0.0	11.886527859551464	0.0
14	0.0	0.0	0.0	12.08906492533319	0.0
15	0.0	0.0	0.0	12.45324626665953	0.0
16	2.140983782047851E-4	0.0	0.0	13.278595514638976	0.0
17	2.140983782047851E-4	0.0	0.0	14.239469036022053	0.0
18	2.140983782047851E-4	0.0	0.0	15.475030776641868	0.0
19	4.281967564095702E-4	0.0	0.0	16.029759674570464	0.0
20	4.281967564095702E-4	0.0	0.0	16.57635283412728	0.0
21	4.281967564095702E-4	0.0	0.0	17.267248300594122	0.0
22	4.281967564095702E-4	0.0	0.0	17.959856554086603	0.0
23	4.281967564095702E-4	0.0	0.0	18.643686774072687	0.0
24	4.281967564095702E-4	0.0	0.0	19.21532944387946	0.0
25	4.281967564095702E-4	0.0	0.0	19.72745276454531	0.0
26	4.281967564095702E-4	0.0	0.0	20.207247230102233	0.0
27	4.281967564095702E-4	0.0	0.0	20.680832842691217	0.0
28	6.422951346143553E-4	0.0	0.0	21.16426698067762	0.0
29	6.422951346143553E-4	0.0	0.0	21.70336669699727	0.0
30	6.422951346143553E-4	0.0	0.0	22.334528715944977	0.0
31	6.422951346143553E-4	0.0	0.0	22.83444842905315	0.0
32	6.422951346143553E-4	0.0	0.0	23.31809666541776	0.0
33	6.422951346143553E-4	0.0	0.0	23.78568752341701	0.0
34	6.422951346143553E-4	0.0	0.0	24.25413477492908	0.0
35	6.422951346143553E-4	0.0	0.0	24.773751538832094	0.0
36	6.422951346143553E-4	0.0	0.0	25.22764010062624	0.0
37	6.422951346143553E-4	0.0	0.0	25.689236204035755	0.0
38	6.422951346143553E-4	0.0	0.0	26.131349355028636	0.0
39	6.422951346143553E-4	0.0	0.0	26.59765562275866	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	30	2.1627238E-6	45.000004	4
AATGACG	30	2.1627238E-6	45.000004	1
CTTGTCG	25	3.887237E-5	45.0	42
CTATGCG	20	7.028982E-4	45.0	1
CGAACGA	20	7.028982E-4	45.0	22
CGACGTC	25	3.887237E-5	45.0	18
CGCGGAA	20	7.028982E-4	45.0	15
GCGAAGT	20	7.028982E-4	45.0	18
GACGTCG	25	3.887237E-5	45.0	19
TAACGCG	20	7.028982E-4	45.0	1
ATCGTAC	25	3.887237E-5	45.0	28
TCGCTAT	25	3.887237E-5	45.0	23
GTCGTGC	20	7.028982E-4	45.0	24
CGAATCC	20	7.028982E-4	45.0	23
AGCGAAC	20	7.028982E-4	45.0	10
TCATCGC	20	7.028982E-4	45.0	32
ATAACGG	40	6.7993824E-9	45.0	2
TAAGCGA	20	7.028982E-4	45.0	25
CCACGAC	20	7.028982E-4	45.0	11
TGTCGGC	25	3.887237E-5	45.0	44
>>END_MODULE