FastQCFastQC Report
Sat 18 Jun 2016
SRR3549898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066912
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC30290.2839034522059926No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC25520.2391949851534147No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.1838952041030563No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT18340.17189796346840225Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC10830.10150790318226809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA1050.045.00000431
CTATCGC207.033059E-445.023
CCGTGAT207.033059E-445.039
TATCGAG207.033059E-445.01
ATCGCGC207.033059E-445.023
ATAGTCG302.165356E-644.9999961
CGAATAT1150.043.0434814
CGACGGT1100.042.95454828
TATACGG700.041.7857132
AACACGT2650.040.7547241
ACACGAC2900.039.56896626
CTAGGGT1650.038.1818164
GCGAGAC3100.037.7419421
GTTAGCG301.1398672E-437.4999961
CGCGAGT301.1398672E-437.49999629
ACGATAG301.1398672E-437.4999961
TAGTCGA301.1398672E-437.49999611
TTGGGTA1450.037.241385
GCAATTG850.037.0588231
TACGGGA3350.036.94034