##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1031842
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50901688436795	34.0	31.0	34.0	31.0	34.0
2	32.67803113267341	34.0	31.0	34.0	31.0	34.0
3	32.738415377548115	34.0	31.0	34.0	31.0	34.0
4	36.14984173933606	37.0	37.0	37.0	35.0	37.0
5	36.04058276364017	37.0	35.0	37.0	35.0	37.0
6	36.02887166833682	37.0	35.0	37.0	35.0	37.0
7	36.192632205318255	37.0	37.0	37.0	35.0	37.0
8	36.193993847895314	37.0	37.0	37.0	35.0	37.0
9	37.96954960158629	39.0	39.0	39.0	35.0	39.0
10	37.521038104671064	39.0	37.0	39.0	35.0	39.0
11	37.3796753766565	39.0	37.0	39.0	34.0	39.0
12	37.34102120285858	39.0	37.0	39.0	34.0	39.0
13	37.294612934926086	39.0	37.0	39.0	34.0	39.0
14	38.532450704662146	40.0	38.0	41.0	34.0	41.0
15	38.59242791047467	40.0	38.0	41.0	34.0	41.0
16	38.69218058578736	40.0	38.0	41.0	35.0	41.0
17	38.725189515449074	40.0	38.0	41.0	35.0	41.0
18	38.25179145644391	39.0	38.0	40.0	35.0	41.0
19	37.787916173212565	38.0	37.0	40.0	34.0	41.0
20	37.24292188145084	38.0	35.0	40.0	34.0	41.0
21	37.19127347016307	38.0	35.0	40.0	34.0	41.0
22	36.99894169843833	38.0	35.0	40.0	34.0	41.0
23	36.87998356337501	38.0	35.0	40.0	33.0	41.0
24	36.66551468151132	37.0	35.0	40.0	33.0	41.0
25	36.521066209749165	37.0	35.0	40.0	33.0	41.0
26	36.655241790894344	37.0	35.0	40.0	33.0	41.0
27	36.6989461564852	38.0	35.0	40.0	33.0	41.0
28	36.648962728789876	38.0	35.0	40.0	33.0	41.0
29	36.52356077771597	38.0	35.0	40.0	33.0	41.0
30	36.23164980685027	38.0	35.0	40.0	32.0	41.0
31	35.71183088108451	38.0	35.0	40.0	30.0	41.0
32	34.97037530939814	38.0	35.0	40.0	23.0	41.0
33	34.04934476402395	38.0	34.0	40.0	17.0	41.0
34	33.342244258326375	38.0	33.0	40.0	12.0	41.0
35	32.889232072352165	38.0	33.0	40.0	10.0	41.0
36	32.707949472884415	38.0	33.0	40.0	9.0	41.0
37	32.49975771484394	38.0	32.0	40.0	8.0	41.0
38	32.36666466377604	37.0	31.0	40.0	8.0	41.0
39	32.33272826653693	37.0	31.0	40.0	8.0	41.0
40	32.229462456461356	37.0	31.0	40.0	8.0	41.0
41	32.106494017494924	37.0	31.0	40.0	8.0	41.0
42	31.975587347675322	37.0	30.0	40.0	7.0	41.0
43	31.857987947767196	37.0	30.0	40.0	7.0	41.0
44	31.723700915450234	37.0	30.0	40.0	7.0	41.0
45	31.679990735015632	37.0	29.0	40.0	7.0	41.0
46	31.633509781536322	36.0	29.0	40.0	7.0	41.0
47	31.607213119838114	36.0	29.0	40.0	7.0	41.0
48	31.582564966341746	36.0	29.0	40.0	7.0	41.0
49	31.49247074649026	36.0	29.0	40.0	7.0	41.0
50	31.397524039533184	36.0	28.0	40.0	7.0	41.0
51	30.836806410283746	35.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	8.0
10	11.0
11	10.0
12	12.0
13	14.0
14	25.0
15	47.0
16	80.0
17	160.0
18	348.0
19	583.0
20	1094.0
21	1884.0
22	3032.0
23	5501.0
24	10756.0
25	23432.0
26	41238.0
27	49360.0
28	44487.0
29	35384.0
30	28266.0
31	25544.0
32	25730.0
33	31895.0
34	57200.0
35	74783.0
36	73439.0
37	95941.0
38	181030.0
39	220416.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.478850444157146	15.587270144072447	17.578854126891517	35.35502528487889
2	50.881530311811304	17.430478697319938	17.40217979109205	14.28581119977671
3	23.83048955169493	17.514406275379372	45.07385820697355	13.581245965952151
4	21.27127990525681	19.81408006264525	44.21946383264104	14.695176199456894
5	18.838640024344812	22.198844396719654	43.728497192399615	15.234018386535922
6	19.804001000153125	25.247857714650113	42.88224359931075	12.065897685886018
7	58.788651750946364	4.74927362910213	32.09192880305318	4.370145816898323
8	58.85377800089549	4.001581637498764	31.79120446735062	5.353435894255129
9	54.35182905910013	5.822887612638369	34.11704505147106	5.708238276790438
10	30.39389751531727	17.842363462623155	39.679815320562646	12.083923701496936
11	23.079890138218836	18.342149282545194	43.91253699694333	14.66542358229264
12	20.74959150722688	16.63064694013231	47.821953361076595	14.797808191564213
13	17.487367251962993	19.710478929913688	48.16144332174887	14.640710496374446
14	14.547769910509556	21.45057092074174	48.16997175924221	15.831687409506495
15	13.559149559719414	19.652039750271843	52.103519724919124	14.685290965089617
16	15.55189651128758	19.105153696011598	48.96079050862438	16.38215928407644
17	15.461378776983297	18.511845805850122	46.21802562795467	19.808749789211912
18	16.787453893134803	18.39089705594461	48.37659254033079	16.4450565105898
19	16.526173580838925	20.616431585455913	46.3665948856511	16.490799948054065
20	17.577303501892732	20.170723812366624	47.06195328354535	15.190019402195299
21	16.92041998678092	20.557217093314677	47.092287385084155	15.430075534820254
22	15.240027058406227	19.346178969260798	46.50653879179177	18.907255180541206
23	14.936007644581245	20.28013978884364	46.345855276292305	18.437997290282816
24	16.552631119880758	18.247464243556667	47.346396056760625	17.853508579801947
25	14.423526082481619	20.006745218744733	46.614307229207576	18.955421469566076
26	14.144219754574827	20.598405569844996	47.898709298516636	17.35866537706354
27	14.894140769613953	20.47241728869342	46.34789047160321	18.285551470089413
28	12.514706708972886	19.829392484508286	47.683560079934715	19.972340726584108
29	13.810060067335892	19.866219828229514	47.48052511915584	18.843194985278757
30	14.525382762089546	19.568112172212413	46.70763547132216	19.198869594375882
31	15.566918190963344	21.116411233502806	42.99369477109868	20.322975804435174
32	15.585816433136083	22.30574060757364	41.81037406889814	20.298068890392134
33	15.661215573702176	22.443552404340974	41.090496413210545	20.8047356087463
34	14.691978035396891	22.165602873308124	40.52248309334181	22.619935997953174
35	14.634217254192016	23.6683523252591	38.12318164990376	23.574248770645116
36	16.60632151046381	23.934187598488915	38.51791262615788	20.941578264889394
37	16.516579088658922	24.85477427745721	36.85942227589108	21.769224357992794
38	15.51070803475726	27.368434314555913	34.94255903520113	22.1782986154857
39	16.828254713415426	24.50869416054008	38.02975649372675	20.63329463231774
40	18.025821782792324	23.336906231768044	37.42472200201193	21.212549983427696
41	15.647356862775503	23.66660787213546	35.74927169082088	24.936763574268152
42	16.701200377577187	23.667577012759704	36.00783840936887	23.623384200294232
43	18.11653334522146	23.426745567635354	35.79423981578575	22.662481271357436
44	16.387489557509774	24.601925488592247	35.074555988223004	23.93602896567498
45	16.537706354267417	24.576437090174657	35.114775324129084	23.77108123142884
46	17.85525303292558	23.58200189563906	36.44433934652786	22.118405724907493
47	14.697599051017502	23.36830638799351	38.68295727446644	23.25113728652255
48	15.237507292783198	21.96499076408985	38.44338571215361	24.354116230973347
49	16.585678815167437	20.63717119481471	40.36499774190235	22.412152248115504
50	15.852039362615594	21.424694866074457	38.89665278211199	23.826612989197958
51	15.005688855464305	21.363348264559885	36.43600473715937	27.19495814281644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	525.0
1	748.0
2	971.0
3	38301.0
4	75631.0
5	51596.5
6	27562.0
7	26727.5
8	25893.0
9	25439.0
10	24985.0
11	24073.5
12	23162.0
13	21755.5
14	20349.0
15	18654.0
16	16959.0
17	15806.5
18	14654.0
19	13393.0
20	12132.0
21	11149.0
22	10166.0
23	9685.0
24	9204.0
25	9004.0
26	9094.5
27	9385.0
28	10131.0
29	10877.0
30	11731.5
31	12586.0
32	13576.5
33	14567.0
34	17346.0
35	20125.0
36	20482.0
37	20839.0
38	23577.0
39	26315.0
40	30315.0
41	34315.0
42	39432.5
43	44550.0
44	51336.0
45	58122.0
46	66752.5
47	75383.0
48	85492.5
49	95602.0
50	94489.0
51	93376.0
52	77326.0
53	61276.0
54	53793.5
55	46311.0
56	41251.0
57	36191.0
58	31388.5
59	26586.0
60	24490.5
61	22395.0
62	20617.5
63	18840.0
64	15758.0
65	12676.0
66	10468.0
67	8260.0
68	6492.5
69	4725.0
70	3961.0
71	3197.0
72	2543.5
73	1890.0
74	1562.5
75	943.5
76	652.0
77	490.5
78	329.0
79	228.5
80	128.0
81	96.0
82	64.0
83	43.0
84	22.0
85	19.5
86	17.0
87	11.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1031842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.27151429743764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85557565410967	24.905583556186354
2	10.555416077016936	7.023893538437442
3	4.4567762080868345	4.448510799835232
4	2.385317237012026	3.1745246622068035
5	1.4654856599370558	2.4379463543642794
6	0.887844927087338	1.7723967111296306
7	0.6395551008644582	1.4895276677687697
8	0.468656918426129	1.2474340289606414
9	0.32617333200640647	0.9767052611364626
>10	3.1383169541924265	25.4637734717159
>50	0.7278903783402478	15.768493521817364
>100	0.08645540543086778	4.94422302180063
>500	0.0038622689711085697	0.8382384586483438
>1k	0.0023767809052975815	1.8466946797722121
>5k	0.0	0.0
>10k+	2.970976131621977E-4	3.6620542662199522
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37047	3.590375270632519	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	4804	0.46557515588626947	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	4484	0.4345626559104979	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	2783	0.26971183572678764	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1722	0.16688601549462032	No Hit
ACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	1321	0.12802347646248166	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCC	1317	0.1276358202127845	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTG	1238	0.11997960928126594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28667179665103765	0.0
2	0.0	0.0	0.0	1.55663367065888	0.0
3	0.0	0.0	0.0	2.158663826438544	0.0
4	0.0	0.0	0.0	2.846753669650974	0.0
5	0.0	0.0	0.0	4.443800504340781	0.0
6	0.0	0.0	0.0	5.28201023024843	0.0
7	0.0	0.0	0.0	6.146386753010636	0.0
8	0.0	0.0	0.0	7.4975626113300295	0.0
9	0.0	0.0	0.0	7.988238509384189	0.0
10	0.0	0.0	0.0	9.073579094473766	0.0
11	0.0	0.0	0.0	10.73701206192421	0.0
12	0.0	0.0	0.0	12.0478716702751	0.0
13	0.0	0.0	0.0	12.519940068343796	0.0
14	0.0	0.0	0.0	12.7130897947554	0.0
15	0.0	0.0	0.0	13.098420106954359	0.0
16	0.0	0.0	0.0	13.94438295785595	0.0
17	0.0	0.0	0.0	14.97235041799035	0.0
18	0.0	0.0	0.0	16.208392370149692	0.0
19	0.0	0.0	0.0	16.877293229002113	0.0
20	9.691406242428589E-5	0.0	0.0	17.520414947249677	0.0
21	9.691406242428589E-5	0.0	0.0	18.318114595063975	0.0
22	1.9382812484857178E-4	0.0	0.0	19.10108330538978	0.0
23	1.9382812484857178E-4	0.0	0.0	19.870484046976184	0.0
24	1.9382812484857178E-4	0.0	0.0	20.484240804309188	0.0
25	1.9382812484857178E-4	0.0	0.0	21.05302943667732	0.0
26	2.907421872728577E-4	0.0	0.0	21.552815256599363	0.0
27	2.907421872728577E-4	0.0	0.0	22.034865803097762	0.0
28	2.907421872728577E-4	0.0	0.0	22.536008419893744	0.0
29	2.907421872728577E-4	0.0	0.0	23.05653384917458	0.0
30	2.907421872728577E-4	0.0	0.0	23.63695216903363	0.0
31	3.8765624969714355E-4	0.0	0.0	24.20903587952419	0.0
32	3.8765624969714355E-4	0.0	0.0	24.73925271504746	0.0
33	3.8765624969714355E-4	0.0	0.0	25.244078066215565	0.0
34	3.8765624969714355E-4	0.0	0.0	25.737370643955177	0.0
35	3.8765624969714355E-4	0.0	0.0	26.25566704980026	0.0
36	3.8765624969714355E-4	0.0	0.0	26.759814002531396	0.0
37	3.8765624969714355E-4	0.0	0.0	27.23837564278252	0.0
38	3.8765624969714355E-4	0.0	0.0	27.73564169708153	0.0
39	3.8765624969714355E-4	0.0	0.0	28.205674899839316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	30	2.165285E-6	45.000004	26
AGCGTTC	35	1.2117744E-7	45.0	7
CGTATGT	20	7.0329505E-4	45.0	44
CGAGTAA	20	7.0329505E-4	45.0	33
CCGTGCG	20	7.0329505E-4	45.0	16
CGTCTAG	20	7.0329505E-4	45.0	40
CTAGTCG	20	7.0329505E-4	45.0	1
GTTCCGC	25	3.8905273E-5	45.0	14
ACGTAGG	55	1.8189894E-12	45.0	2
ATCGGTC	20	7.0329505E-4	45.0	26
CGACTTA	20	7.0329505E-4	45.0	25
CACGTAG	25	3.8905273E-5	45.0	1
TAAATCG	20	7.0329505E-4	45.0	27
ACATACC	20	7.0329505E-4	45.0	30
GTGCGAC	20	7.0329505E-4	45.0	8
CGTTTTT	26035	0.0	44.37776	1
CACAACG	125	0.0	41.399998	12
CGCACGG	105	0.0	40.714287	2
ACGGGTC	45	1.9279469E-8	40.0	5
TCATCGA	45	1.9279469E-8	40.0	16
>>END_MODULE