FastQCFastQC Report
Sat 18 Jun 2016
SRR3549893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592794
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT599033.7608755432278125No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC64920.4075856639339425TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC49260.30926786514765875TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG43510.27316777938641157No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC42980.2698402932205923No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC37030.2324845523024321No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT30840.19362202519597638TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC19210.12060567782148854TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA16440.10321485389824421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG207.0341065E-445.013
CTCGTCA207.0341065E-445.012
CATGCGA207.0341065E-445.025
CACGCGA207.0341065E-445.015
TATTGCG502.1827873E-1145.01
TGCGCGA207.0341065E-445.018
GTTAACG253.891487E-545.01
GGACGTA207.0341065E-445.08
TACTCGT207.0341065E-445.013
CTCGAAT207.0341065E-445.035
TTACGAG453.8562575E-1045.01
AATAGCG207.0341065E-445.01
TCGACCA207.0341065E-445.030
ATCGAAC253.891487E-545.029
TATCGCG600.044.9999961
GCGTACG302.1660326E-644.9999961
TCGAACG302.1660326E-644.9999961
GTTATCG302.1660326E-644.99999627
CGTTTTT407900.044.255331
CGAATAT750.042.014
Produced by FastQC (version 0.11.5)