##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1592794
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55763080473683	34.0	31.0	34.0	31.0	34.0
2	32.713402360882824	34.0	31.0	34.0	31.0	34.0
3	32.76933426419236	34.0	31.0	34.0	31.0	34.0
4	36.18679377245268	37.0	37.0	37.0	35.0	37.0
5	36.073327749853405	37.0	35.0	37.0	35.0	37.0
6	36.05801315173211	37.0	35.0	37.0	35.0	37.0
7	36.22795854328934	37.0	37.0	37.0	35.0	37.0
8	36.1999247862561	37.0	37.0	37.0	35.0	37.0
9	37.987262634088275	39.0	39.0	39.0	35.0	39.0
10	37.54163061889987	39.0	37.0	39.0	35.0	39.0
11	37.459636337153455	39.0	37.0	39.0	35.0	39.0
12	37.44634962211058	39.0	37.0	39.0	35.0	39.0
13	37.40940322477358	39.0	37.0	39.0	35.0	39.0
14	38.680587696839645	40.0	38.0	41.0	35.0	41.0
15	38.72071969130974	40.0	38.0	41.0	35.0	41.0
16	38.82526679532946	40.0	38.0	41.0	35.0	41.0
17	38.839009313194296	40.0	38.0	41.0	35.0	41.0
18	38.350582058947985	39.0	38.0	41.0	35.0	41.0
19	37.900572202055	38.0	37.0	41.0	35.0	41.0
20	37.34242595087626	38.0	35.0	41.0	34.0	41.0
21	37.29805298111369	38.0	35.0	41.0	34.0	41.0
22	37.11309120953494	38.0	35.0	40.0	34.0	41.0
23	36.987298420260245	38.0	35.0	40.0	33.0	41.0
24	36.77124160437571	38.0	35.0	40.0	33.0	41.0
25	36.6446709367313	38.0	35.0	40.0	33.0	41.0
26	36.765921393475864	38.0	35.0	40.0	33.0	41.0
27	36.79547386542139	38.0	35.0	40.0	33.0	41.0
28	36.74214995787277	38.0	35.0	40.0	33.0	41.0
29	36.62618078671818	38.0	35.0	40.0	33.0	41.0
30	36.337619930763175	38.0	35.0	40.0	33.0	41.0
31	35.84243850742783	38.0	35.0	40.0	31.0	41.0
32	35.12780372100849	38.0	35.0	40.0	23.0	41.0
33	34.249433385610445	38.0	35.0	41.0	18.0	41.0
34	33.532964714834435	38.0	34.0	41.0	12.0	41.0
35	33.10295493328076	38.0	33.0	41.0	10.0	41.0
36	32.88666707684735	38.0	33.0	41.0	10.0	41.0
37	32.66048151863957	38.0	32.0	41.0	9.0	41.0
38	32.51960893875793	38.0	31.0	41.0	8.0	41.0
39	32.45986486639201	38.0	31.0	40.0	8.0	41.0
40	32.3293282119345	38.0	31.0	40.0	8.0	41.0
41	32.19451919080559	37.0	31.0	40.0	8.0	41.0
42	32.07492745452331	37.0	30.0	40.0	8.0	41.0
43	31.95861297820057	37.0	30.0	40.0	7.0	41.0
44	31.846108787451485	37.0	30.0	40.0	7.0	41.0
45	31.821386820894602	37.0	29.0	40.0	8.0	41.0
46	31.77015106787193	37.0	29.0	40.0	8.0	41.0
47	31.745196805111018	37.0	29.0	40.0	8.0	41.0
48	31.706952060341763	36.0	29.0	40.0	8.0	41.0
49	31.596738812426466	36.0	29.0	40.0	8.0	41.0
50	31.519252960520948	36.0	28.0	40.0	8.0	41.0
51	30.977462873416147	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	12.0
10	9.0
11	17.0
12	25.0
13	24.0
14	33.0
15	61.0
16	123.0
17	237.0
18	463.0
19	958.0
20	1654.0
21	2862.0
22	4501.0
23	7820.0
24	15042.0
25	32251.0
26	59414.0
27	77251.0
28	73062.0
29	57647.0
30	44321.0
31	39172.0
32	38903.0
33	47171.0
34	80219.0
35	103794.0
36	112805.0
37	146239.0
38	277098.0
39	369387.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.58870889769801	14.543311941154977	17.35943254432149	35.50854661682553
2	50.301922282479715	18.6660673006051	17.61671628597295	13.415294130942232
3	23.668409097472743	18.755532730535148	44.75537954060601	12.820678631386107
4	21.69696771836157	18.78836811288842	44.90856946974938	14.606094699000623
5	19.800049472813182	21.70261816656768	43.1515312086811	15.34580115193804
6	19.81059697613125	25.196729771709336	42.88489283611063	12.10778041604878
7	59.436185721442946	4.011441529789791	32.645401728032624	3.9069710207346335
8	59.15447948698953	4.7972933097437584	31.54004849340216	4.508178709864553
9	54.63782510481582	5.562552345124354	33.330424398886485	6.469198151173347
10	30.194048947949327	18.751640199548717	39.29434691491806	11.759963937583894
11	23.599034150053303	18.416066358863734	43.26491686935034	14.719982621732628
12	21.640526019058335	15.856601669770228	46.89834341415148	15.604528897019954
13	18.58746328778235	18.098008907617682	47.71910240746763	15.595425397132335
14	14.530001996491698	21.625520939933224	46.760221346891065	17.084255716684016
15	13.723557471964359	19.59110845470287	51.50314478834049	15.182189284992283
16	15.341720272678074	18.74040208589435	49.280258464057496	16.63761917737008
17	15.43407370946902	18.507791968076223	46.814591215185395	19.243543107269364
18	15.347182372610646	19.627459671495497	47.68840163888111	17.336956317012746
19	17.816302673164262	19.912681740388276	45.30133840283175	16.96967718361571
20	19.45405369432582	19.521796290041273	46.23950115331926	14.784648862313645
21	18.048787225466693	19.9527371398938	46.36286927248596	15.635606362153547
22	16.401556007870447	19.040440885638695	45.126300073958085	19.43170303253277
23	16.266635861260152	20.28429288407666	46.02340290081454	17.425668353848643
24	17.290371510691276	17.963904936859382	46.480021898625935	18.265701653823406
25	16.33513185006975	20.307648069995242	44.86763511163402	18.489584968300985
26	15.269582883913424	20.66984180000678	46.51348510855767	17.54709020752213
27	17.634672154716803	20.761253495430044	45.27923887207009	16.324835477783065
28	14.641253043394187	20.42473791337737	46.61023333839781	18.32377570483063
29	16.277120581820373	18.446704344692407	47.33870167768085	17.937473395806364
30	17.186654394730265	19.084891078193415	44.998411596226504	18.730042930849816
31	16.668319945956604	20.123317892960422	42.53117477840826	20.677187382674724
32	17.233301983809582	21.47829537278518	43.23170479044999	18.056697852955246
33	17.76456968069945	21.807716503201295	40.128164721866106	20.299549094233154
34	16.640883880778055	22.19590229496093	39.72830133714717	21.434912487113838
35	17.53308965252255	21.977732211447307	38.75008318715415	21.739094948876
36	18.28773840182723	24.582588834463216	36.67410851622997	20.455564247479586
37	18.64151924228745	24.465875687628156	37.40703443132006	19.485570638764337
38	17.122741547243397	24.753044022014144	36.309089562115375	21.815124868627077
39	18.468050482359928	23.13619965921519	36.89108572734453	21.504664131080354
40	18.40884634171148	23.216310458226236	37.92160191462298	20.453241285439297
41	16.60114239506176	24.57875908623463	36.83219550048531	21.9879030182183
42	18.056823418470938	24.176823870506794	37.24831961948626	20.518033091536005
43	19.463533890760512	24.263777990123018	35.89365605345073	20.379032065665744
44	18.377454962788658	23.933917380401986	35.279075636899684	22.40955201990967
45	18.068752142461612	22.62948002064297	34.88994810377237	24.411819733123053
46	18.518716167941367	23.69961212812203	35.98695123160936	21.794720472327246
47	16.56592126791035	23.003979171192256	39.4531245095097	20.976975051387686
48	16.885046026039777	21.661683808452317	38.45732718731989	22.995942978188015
49	16.650929122033357	21.297229899158335	40.21650006215493	21.83534091665338
50	16.464966593294548	21.099652560218082	38.99041558418728	23.444965262300084
51	16.36495366004643	20.571398435704804	37.12118453484883	25.94246336939994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	538.0
1	955.5
2	1373.0
3	61499.0
4	121625.0
5	84592.5
6	47560.0
7	45311.5
8	43063.0
9	41536.5
10	40010.0
11	37422.0
12	34834.0
13	31965.0
14	29096.0
15	26513.5
16	23931.0
17	22077.0
18	20223.0
19	18831.5
20	17440.0
21	16208.5
22	14977.0
23	13949.0
24	12921.0
25	13379.5
26	14206.5
27	14575.0
28	15622.0
29	16669.0
30	18287.0
31	19905.0
32	22359.5
33	24814.0
34	26268.0
35	27722.0
36	30493.0
37	33264.0
38	35537.5
39	37811.0
40	43447.0
41	49083.0
42	53963.0
43	58843.0
44	66487.5
45	74132.0
46	94456.5
47	114781.0
48	122023.5
49	129266.0
50	129536.5
51	129807.0
52	110267.0
53	90727.0
54	80700.0
55	70673.0
56	63361.0
57	56049.0
58	53924.5
59	51800.0
60	51154.5
61	50509.0
62	46496.0
63	42483.0
64	35886.0
65	29289.0
66	24198.0
67	19107.0
68	15331.0
69	11555.0
70	9033.0
71	6511.0
72	5652.0
73	4793.0
74	4058.0
75	2606.5
76	1890.0
77	1499.5
78	1109.0
79	872.0
80	635.0
81	387.5
82	140.0
83	78.0
84	16.0
85	34.0
86	52.0
87	35.0
88	18.0
89	12.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1592794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.318992475676264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35836563555523	22.847897206836596
2	10.365169819095119	6.285230115085032
3	4.119651071608272	3.7471100952750858
4	2.1541567015796055	2.6124744330647864
5	1.3675928714988113	2.0732018990380476
6	0.8705941433856852	1.5837322369607048
7	0.5572528369960073	1.1826741200351836
8	0.47251247667589374	1.1460881779999663
9	0.33915506308569104	0.9254555825204317
>10	3.314417603155686	24.759778892353662
>50	0.9616155412555828	19.22600762084916
>100	0.11042031080209705	6.021095937888379
>500	0.0052883286665252145	1.0556574717724665
>1k	0.0033845303465761378	2.2753715542857575
>5k	2.115331466610086E-4	0.4163625945775731
>10k+	2.115331466610086E-4	3.841862061457233
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59903	3.7608755432278125	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	6492	0.4075856639339425	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4926	0.30926786514765875	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	4351	0.27316777938641157	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	4298	0.2698402932205923	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	3703	0.2324845523024321	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	3084	0.19362202519597638	TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1921	0.12060567782148854	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1644	0.10321485389824421	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.278275784564733E-5	0.0	0.0	0.2047973560925016	0.0
2	6.278275784564733E-5	0.0	0.0	1.1889170853230235	0.0
3	6.278275784564733E-5	0.0	0.0	1.6642453449724195	0.0
4	6.278275784564733E-5	0.0	0.0	2.2081323761892624	0.0
5	6.278275784564733E-5	0.0	0.0	3.703240971525508	0.0
6	6.278275784564733E-5	0.0	0.0	4.343813449824648	0.0
7	6.278275784564733E-5	0.0	0.0	5.083143206214991	0.0
8	6.278275784564733E-5	0.0	0.0	6.135193879434503	0.0
9	6.278275784564733E-5	0.0	0.0	6.480938526890483	0.0
10	6.278275784564733E-5	0.0	0.0	7.639217626384831	0.0
11	6.278275784564733E-5	0.0	0.0	8.931914610426709	0.0
12	6.278275784564733E-5	0.0	0.0	10.256568018212022	0.0
13	6.278275784564733E-5	0.0	0.0	10.674261706159115	0.0
14	6.278275784564733E-5	0.0	0.0	10.8417661040913	0.0
15	6.278275784564733E-5	0.0	0.0	11.2023902651567	0.0
16	1.2556551569129466E-4	0.0	0.0	11.881448574015222	0.0
17	1.2556551569129466E-4	0.0	0.0	12.702082001815677	0.0
18	1.2556551569129466E-4	0.0	0.0	13.613248166429557	0.0
19	1.2556551569129466E-4	0.0	0.0	14.224375531299088	0.0
20	1.2556551569129466E-4	0.0	0.0	14.752001828233908	0.0
21	1.2556551569129466E-4	0.0	0.0	15.390879172071216	0.0
22	1.2556551569129466E-4	0.0	0.0	16.117401245861046	0.0
23	1.2556551569129466E-4	0.0	0.0	16.79024406169285	0.0
24	1.2556551569129466E-4	0.0	0.0	17.321825672371943	0.0
25	1.2556551569129466E-4	0.0	0.0	17.77499161850183	0.0
26	1.2556551569129466E-4	0.0	0.0	18.20593246835435	0.0
27	1.2556551569129466E-4	0.0	0.0	18.628648776929094	0.0
28	1.2556551569129466E-4	0.0	0.0	19.04784925106448	0.0
29	1.2556551569129466E-4	0.0	0.0	19.482870980176973	0.0
30	1.2556551569129466E-4	0.0	0.0	19.980047639556652	0.0
31	1.2556551569129466E-4	0.0	0.0	20.46058686810724	0.0
32	1.2556551569129466E-4	0.0	0.0	20.9183987383177	0.0
33	1.2556551569129466E-4	0.0	0.0	21.328558495323314	0.0
34	1.2556551569129466E-4	0.0	0.0	21.734323459279732	0.0
35	1.2556551569129466E-4	0.0	0.0	22.16231351951351	0.0
36	1.2556551569129466E-4	0.0	0.0	22.61924643111413	0.0
37	1.2556551569129466E-4	0.0	0.0	23.074358642737227	0.0
38	1.2556551569129466E-4	0.0	0.0	23.483074396312393	0.0
39	1.2556551569129466E-4	0.0	0.0	23.897566163609355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	20	7.0341065E-4	45.0	13
CTCGTCA	20	7.0341065E-4	45.0	12
CATGCGA	20	7.0341065E-4	45.0	25
CACGCGA	20	7.0341065E-4	45.0	15
TATTGCG	50	2.1827873E-11	45.0	1
TGCGCGA	20	7.0341065E-4	45.0	18
GTTAACG	25	3.891487E-5	45.0	1
GGACGTA	20	7.0341065E-4	45.0	8
TACTCGT	20	7.0341065E-4	45.0	13
CTCGAAT	20	7.0341065E-4	45.0	35
TTACGAG	45	3.8562575E-10	45.0	1
AATAGCG	20	7.0341065E-4	45.0	1
TCGACCA	20	7.0341065E-4	45.0	30
ATCGAAC	25	3.891487E-5	45.0	29
TATCGCG	60	0.0	44.999996	1
GCGTACG	30	2.1660326E-6	44.999996	1
TCGAACG	30	2.1660326E-6	44.999996	1
GTTATCG	30	2.1660326E-6	44.999996	27
CGTTTTT	40790	0.0	44.25533	1
CGAATAT	75	0.0	42.0	14
>>END_MODULE