FastQCFastQC Report
Sat 18 Jun 2016
SRR3549892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931647
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411444.416264958723637No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC28680.3078419186666194No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC24360.26147242464152193No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT18500.198573064690811No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11220.12043188031518376No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT11200.12021720673173422No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG10820.11613840864619325No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC10780.1157090614792942No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC10520.11291830489445036No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTTT10480.11248895772755133No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA9730.10443869834819411No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA9320.10003788988747883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT453.8380676E-1045.00000425
AGTACGG453.8380676E-1045.0000042
AGTCACG207.032599E-445.01
CGTGAAT207.032599E-445.015
TAGCGCG406.8121153E-945.01
GCGCCTA207.032599E-445.09
ATATGCG253.890234E-545.013
CGCCTAG207.032599E-445.010
TACGCTA253.890234E-545.021
ACGATAC207.032599E-445.038
GCTTACG351.2115925E-745.01
TGTTACG253.890234E-545.01
ACTAACG207.032599E-445.01
CGTACGA207.032599E-445.011
GGATTAC253.890234E-545.08
GGTTCGA207.032599E-445.041
TCGACAG351.2115925E-745.01
CGATATC253.890234E-545.039
TGTCGCC207.032599E-445.021
AATACGG351.2115925E-745.02