##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	931647
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.584876031372396	34.0	31.0	34.0	31.0	34.0
2	32.751112814188204	34.0	31.0	34.0	31.0	34.0
3	32.786980476510955	34.0	31.0	34.0	31.0	34.0
4	36.19485169812172	37.0	37.0	37.0	35.0	37.0
5	36.08560001803258	37.0	35.0	37.0	35.0	37.0
6	36.07509067275481	37.0	35.0	37.0	35.0	37.0
7	36.22051055818352	37.0	37.0	37.0	35.0	37.0
8	36.23240562144245	37.0	37.0	37.0	35.0	37.0
9	38.02168417866424	39.0	39.0	39.0	35.0	39.0
10	37.62592054716003	39.0	38.0	39.0	35.0	39.0
11	37.48593297676051	39.0	37.0	39.0	35.0	39.0
12	37.325709201017126	39.0	37.0	39.0	35.0	39.0
13	37.270277261666706	39.0	37.0	39.0	34.0	39.0
14	38.51905067047927	40.0	38.0	41.0	34.0	41.0
15	38.55480777590654	40.0	38.0	41.0	34.0	41.0
16	38.6311585825962	40.0	38.0	41.0	35.0	41.0
17	38.616398700366126	40.0	38.0	41.0	34.0	41.0
18	38.08062817783989	39.0	38.0	40.0	34.0	41.0
19	37.572106173260906	37.0	37.0	40.0	34.0	41.0
20	36.98859331914341	37.0	35.0	40.0	34.0	41.0
21	36.936421198157674	37.0	35.0	40.0	34.0	41.0
22	36.75603742619254	37.0	35.0	40.0	33.0	41.0
23	36.64709058259191	36.0	35.0	40.0	33.0	41.0
24	36.450389471548775	36.0	35.0	40.0	33.0	41.0
25	36.307595044045655	36.0	35.0	40.0	33.0	41.0
26	36.434609889797315	36.0	35.0	40.0	33.0	41.0
27	36.46545955710693	36.0	35.0	40.0	33.0	41.0
28	36.38156297395902	36.0	35.0	40.0	33.0	41.0
29	36.23831558519482	36.0	35.0	40.0	33.0	41.0
30	35.87777774199885	36.0	35.0	40.0	31.0	41.0
31	35.3044865705573	36.0	35.0	40.0	30.0	41.0
32	34.54150338057226	36.0	35.0	40.0	22.0	41.0
33	33.60077797706642	36.0	34.0	40.0	16.0	41.0
34	32.788671030980616	36.0	33.0	40.0	12.0	41.0
35	32.273372854740046	36.0	31.0	40.0	10.0	41.0
36	32.04288641513363	36.0	31.0	40.0	8.0	41.0
37	31.798593244007655	35.0	30.0	40.0	8.0	41.0
38	31.695502695763523	35.0	30.0	40.0	8.0	41.0
39	31.65899852626585	35.0	30.0	40.0	8.0	41.0
40	31.538000981058275	35.0	29.0	40.0	7.0	41.0
41	31.400475716660925	35.0	29.0	40.0	7.0	41.0
42	31.262305358145305	35.0	27.0	40.0	7.0	41.0
43	31.137513457350263	35.0	27.0	40.0	7.0	41.0
44	31.017408954249838	35.0	25.0	40.0	7.0	41.0
45	30.98581973644524	35.0	25.0	40.0	7.0	41.0
46	30.93141823029538	35.0	24.0	40.0	7.0	41.0
47	30.906985156395073	35.0	25.0	40.0	7.0	41.0
48	30.83217248593083	35.0	25.0	40.0	7.0	41.0
49	30.693434315786988	35.0	24.0	40.0	7.0	41.0
50	30.57652200887246	35.0	24.0	40.0	7.0	41.0
51	29.99812375288065	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	9.0
11	11.0
12	14.0
13	14.0
14	22.0
15	50.0
16	111.0
17	189.0
18	379.0
19	670.0
20	1123.0
21	1964.0
22	2895.0
23	5153.0
24	9808.0
25	20643.0
26	36819.0
27	47168.0
28	44669.0
29	36602.0
30	30710.0
31	27647.0
32	26992.0
33	31997.0
34	61423.0
35	73135.0
36	65980.0
37	82188.0
38	144192.0
39	178950.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.988017993939764	14.983786777609975	17.588850712769965	38.4393445156803
2	52.72812556687243	17.21800209736091	17.76606375590755	12.28780857985911
3	22.940233800999735	17.277466679976428	47.176237351700806	12.606062167323032
4	21.110034165300807	18.77213150474375	46.163729395361116	13.954104934594325
5	18.838250968446204	21.240663040829844	45.81488482225564	14.106201168468315
6	19.444489168107662	24.371140571482545	45.015011050322705	11.169359210087082
7	57.9130292911371	4.514048775984896	33.913274019022225	3.6596479138557845
8	58.262947232159824	3.8621924398404115	33.755488935186825	4.119371392812943
9	54.124040543252974	5.426304168853654	35.3388139499188	5.110841337974576
10	32.36322340972493	17.05270343810478	40.13172371080463	10.452349441365667
11	25.471986707411716	16.95524163121869	43.716665217619976	13.856106443749619
12	23.239810786703547	15.491275128884652	47.16346427348556	14.10544981092624
13	19.37257351765207	18.12220723085031	47.57971635179419	14.925502899703428
14	14.980566673858231	19.873836335006715	48.898026827757725	16.247570163377333
15	15.47184716958247	17.973223763936343	52.02077610940624	14.534152957074944
16	17.845492981783874	17.183868997592437	50.15676538431401	14.813872636309675
17	17.07084335590626	17.856978018498424	47.35173300617079	17.720445619424524
18	17.424625421431077	18.06499672086101	48.963824281085	15.546553576622907
19	18.3068265126169	19.701882794663643	46.486705801660925	15.504584891058522
20	19.041761525556353	19.610861195281046	47.73782344600476	13.609553833157836
21	18.712666922128232	19.851080935161065	47.29752792634979	14.138724216360918
22	16.9388191020848	19.054212593396425	46.69772993419181	17.30923837032696
23	16.91660038619778	18.37230195556901	48.058760453261804	16.65233720497141
24	18.26120837613388	17.063866464444153	48.14398586589127	16.530939293530704
25	16.373905567237376	18.527618293194738	47.015768848072284	18.0827072914956
26	15.22486521182379	19.342733889552587	47.37663514185094	18.055765756772683
27	15.840871059532205	19.28960217764883	48.16878066477969	16.70074609803928
28	14.220729525238637	19.268993513637675	48.53211570476801	17.97816125635568
29	17.09445745008571	18.655671085722382	47.22078212026658	17.029089343925328
30	17.546882027205584	19.34069449050982	47.27788529346416	15.834538188820444
31	18.80905536109707	20.74594776777041	43.99166207801882	16.4533347931137
32	19.818021203309836	21.413367938714988	42.681079851059465	16.087531006915707
33	19.578230810596718	22.29964782798635	41.60814128097874	16.51398008043819
34	17.71411274871276	23.99009496085964	40.02567496058057	18.270117329847036
35	18.782435836749325	24.210135383895405	38.42871817329954	18.578710606055726
36	19.63941278187983	27.010659616786185	36.58563812259364	16.764289478740338
37	18.83685559015378	27.648562170006453	36.42366690388098	17.090915335958794
38	19.397905000499115	26.898814679808986	34.83272097693654	18.870559342755357
39	20.143681029402767	25.997400302904428	36.34015887991911	17.518759787773696
40	19.942209871335386	25.090941096788804	36.178187661206444	18.788661370669363
41	17.44587810619258	26.534728282278586	34.903455922683165	21.11593768884567
42	18.693668309992947	25.30175055573624	34.94370721957995	21.060873914690863
43	19.388030015660437	23.772630620825268	35.82805504660026	21.011284316914026
44	18.02839487488287	23.783364299997743	35.77427931394616	22.413961511173223
45	18.765905970823713	24.05031090101723	35.61434749427627	21.56943563388279
46	18.859074306040807	24.828609977813485	37.107509603959436	19.20480611218627
47	17.749963237148833	23.63427349629205	38.97270103376064	19.643062232798474
48	17.34873830968167	22.392601489619995	39.455394586146895	20.80326561455143
49	18.736817700266304	21.35368868251602	40.0214888257033	19.888004791514383
50	17.532069549947565	21.53787861711571	39.74069577855131	21.189356054385406
51	16.454086150655776	21.93513208328906	38.0310353599593	23.57974640609587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	761.0
2	1157.0
3	45051.0
4	88945.0
5	59251.0
6	29557.0
7	27090.0
8	24623.0
9	23727.0
10	22831.0
11	22081.5
12	21332.0
13	19932.5
14	18533.0
15	17079.0
16	15625.0
17	14117.5
18	12610.0
19	11581.0
20	10552.0
21	9667.5
22	8783.0
23	8351.5
24	7920.0
25	8341.5
26	8449.0
27	8135.0
28	8870.0
29	9605.0
30	10679.5
31	11754.0
32	12061.5
33	12369.0
34	14461.0
35	16553.0
36	17211.5
37	17870.0
38	18518.0
39	19166.0
40	22939.0
41	26712.0
42	30277.5
43	33843.0
44	38869.0
45	43895.0
46	49616.5
47	55338.0
48	61466.0
49	67594.0
50	67014.0
51	66434.0
52	57999.5
53	49565.0
54	44667.0
55	39769.0
56	38324.0
57	36879.0
58	35259.5
59	33640.0
60	32662.5
61	31685.0
62	29149.0
63	26613.0
64	23585.0
65	20557.0
66	16136.0
67	11715.0
68	9612.0
69	7509.0
70	6178.5
71	4848.0
72	4171.0
73	3494.0
74	2610.5
75	1609.5
76	1492.0
77	1128.5
78	765.0
79	568.0
80	371.0
81	226.0
82	81.0
83	49.0
84	17.0
85	17.0
86	17.0
87	21.0
88	25.0
89	18.0
90	11.0
91	6.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	931647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.465681112926994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30264638450669	25.869857130892594
2	9.878894951493974	6.6120789638960344
3	3.6834730720694986	3.6980980565379413
4	1.795486153542306	2.403486682284907
5	1.0496720465063736	1.7563994990767888
6	0.6946950285982969	1.394906537868378
7	0.46173454019745686	1.081658261675044
8	0.3612871850717862	0.9672577380639548
9	0.28633616683800095	0.862419136544856
>10	3.8640831867584824	32.29437029812469
>50	0.5345168762568101	11.176746337759914
>100	0.07963677136446524	4.892923973530065
>500	0.004260403406329207	0.9796157382729098
>1k	0.0029495100505356046	1.4977197180760027
>5k	0.0	0.0
>10k+	3.2772333894840047E-4	4.512461927395947
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41144	4.416264958723637	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	2868	0.3078419186666194	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	2436	0.26147242464152193	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	1850	0.198573064690811	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1122	0.12043188031518376	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT	1120	0.12021720673173422	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1082	0.11613840864619325	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCC	1078	0.1157090614792942	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	1052	0.11291830489445036	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTTT	1048	0.11248895772755133	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	973	0.10443869834819411	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	932	0.10003788988747883	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.073367917247627E-4	0.0	0.0	0.21177549007295682	0.0
2	1.073367917247627E-4	0.0	0.0	1.0311845580997954	0.0
3	1.073367917247627E-4	0.0	0.0	1.4925180889328253	0.0
4	1.073367917247627E-4	0.0	0.0	1.970381485691469	0.0
5	1.073367917247627E-4	0.0	0.0	3.097525135593202	0.0
6	1.073367917247627E-4	0.0	0.0	3.66157997610683	0.0
7	1.073367917247627E-4	0.0	0.0	4.289070860529793	0.0
8	1.073367917247627E-4	0.0	0.0	5.180717589387397	0.0
9	1.073367917247627E-4	0.0	0.0	5.517540441819702	0.0
10	1.073367917247627E-4	0.0	0.0	6.258486315095739	0.0
11	1.073367917247627E-4	0.0	0.0	7.454218174909595	0.0
12	1.073367917247627E-4	0.0	0.0	8.367117588528702	0.0
13	1.073367917247627E-4	0.0	0.0	8.711990700340365	0.0
14	1.073367917247627E-4	0.0	0.0	8.864838291756428	0.0
15	1.073367917247627E-4	0.0	0.0	9.150783504911194	0.0
16	1.073367917247627E-4	0.0	0.0	9.741350532980839	0.0
17	1.073367917247627E-4	0.0	0.0	10.432170124521413	0.0
18	1.073367917247627E-4	0.0	0.0	11.225496352159134	0.0
19	1.073367917247627E-4	0.0	0.0	11.689513302785283	0.0
20	1.073367917247627E-4	0.0	0.0	12.158360409039046	0.0
21	1.073367917247627E-4	0.0	0.0	12.730358172140306	0.0
22	1.073367917247627E-4	0.0	0.0	13.31233825687197	0.0
23	1.073367917247627E-4	0.0	0.0	13.87982787472079	0.0
24	1.073367917247627E-4	0.0	0.0	14.348674980974554	0.0
25	1.073367917247627E-4	0.0	0.0	14.771259929994944	0.0
26	1.073367917247627E-4	0.0	0.0	15.143289250112971	0.0
27	1.073367917247627E-4	0.0	0.0	15.485049594964616	0.0
28	1.073367917247627E-4	0.0	0.0	15.854610168872975	0.0
29	2.146735834495254E-4	0.0	0.0	16.238661209664176	0.0
30	2.146735834495254E-4	0.0	0.0	16.677239340651557	0.0
31	2.146735834495254E-4	0.0	0.0	17.091237346333966	0.0
32	2.146735834495254E-4	0.0	0.0	17.49568237755287	0.0
33	2.146735834495254E-4	0.0	0.0	17.913115160570474	0.0
34	2.146735834495254E-4	0.0	0.0	18.313159383328664	0.0
35	3.2201037517428814E-4	0.0	0.0	18.728982114470394	0.0
36	3.2201037517428814E-4	0.0	0.0	19.111208429802275	0.0
37	3.2201037517428814E-4	0.0	0.0	19.49944560547074	0.0
38	3.2201037517428814E-4	0.0	0.0	19.903568626314474	0.0
39	3.2201037517428814E-4	0.0	0.0	20.35352445722468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	45	3.8380676E-10	45.000004	25
AGTACGG	45	3.8380676E-10	45.000004	2
AGTCACG	20	7.032599E-4	45.0	1
CGTGAAT	20	7.032599E-4	45.0	15
TAGCGCG	40	6.8121153E-9	45.0	1
GCGCCTA	20	7.032599E-4	45.0	9
ATATGCG	25	3.890234E-5	45.0	13
CGCCTAG	20	7.032599E-4	45.0	10
TACGCTA	25	3.890234E-5	45.0	21
ACGATAC	20	7.032599E-4	45.0	38
GCTTACG	35	1.2115925E-7	45.0	1
TGTTACG	25	3.890234E-5	45.0	1
ACTAACG	20	7.032599E-4	45.0	1
CGTACGA	20	7.032599E-4	45.0	11
GGATTAC	25	3.890234E-5	45.0	8
GGTTCGA	20	7.032599E-4	45.0	41
TCGACAG	35	1.2115925E-7	45.0	1
CGATATC	25	3.890234E-5	45.0	39
TGTCGCC	20	7.032599E-4	45.0	21
AATACGG	35	1.2115925E-7	45.0	2
>>END_MODULE