SRR3549891_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982161
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39428	4.014413115568629	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3520	0.3583933794968442	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2827	0.28783468290840303	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	2371	0.2414064496554027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	2239	0.2279666979242711	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	1770	0.1802148527583563	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1592	0.16209155118152727	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1053	0.107212564946073	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	998	0.10161266839143482	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	35	1.2116834E-7	45.000004	1
CGGGTAC	40	6.8139343E-9	45.0	6
CGAACCG	20	7.032784E-4	45.0	1
ATTAGCG	45	3.8380676E-10	45.0	1
CTATCGT	85	0.0	45.0	31
ACCGCAA	20	7.032784E-4	45.0	33
ACATTCG	20	7.032784E-4	45.0	29
CGCGAAT	20	7.032784E-4	45.0	32
CCGTAGA	20	7.032784E-4	45.0	25
CCGTAAG	20	7.032784E-4	45.0	43
TACGACA	20	7.032784E-4	45.0	17
ACGCTAG	25	3.8903898E-5	45.0	1
TCGTAGC	20	7.032784E-4	45.0	28
CTATTAC	25	3.8903898E-5	45.0	37
TGTAGCG	20	7.032784E-4	45.0	1
CGCACTC	20	7.032784E-4	45.0	44
AATCGAC	25	3.8903898E-5	45.0	24
TCTATCG	85	0.0	45.0	30
CGTTTTT	23950	0.0	44.351772	1
ATCGTGT	90	0.0	42.5	33