##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982161
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54046434342231	34.0	31.0	34.0	31.0	34.0
2	32.695566205540636	34.0	31.0	34.0	31.0	34.0
3	32.76044151620763	34.0	31.0	34.0	31.0	34.0
4	36.17291564214014	37.0	37.0	37.0	35.0	37.0
5	36.057876458136704	37.0	35.0	37.0	35.0	37.0
6	36.04109611356998	37.0	35.0	37.0	35.0	37.0
7	36.19769467531291	37.0	37.0	37.0	35.0	37.0
8	36.18187853111659	37.0	37.0	37.0	35.0	37.0
9	37.95796615829788	39.0	39.0	39.0	35.0	39.0
10	37.56535232003714	39.0	37.0	39.0	35.0	39.0
11	37.42806729242965	39.0	37.0	39.0	34.0	39.0
12	37.34695228175421	39.0	37.0	39.0	35.0	39.0
13	37.292466306440595	39.0	37.0	39.0	34.0	39.0
14	38.52335615036639	40.0	38.0	41.0	34.0	41.0
15	38.586055646681146	40.0	38.0	41.0	34.0	41.0
16	38.67984373234123	40.0	38.0	41.0	35.0	41.0
17	38.68475535070116	40.0	38.0	41.0	35.0	41.0
18	38.25013516113957	39.0	38.0	41.0	35.0	41.0
19	37.77682783168951	38.0	37.0	41.0	34.0	41.0
20	37.24530601398345	38.0	35.0	41.0	34.0	41.0
21	37.200312372411446	38.0	35.0	41.0	34.0	41.0
22	37.010231520086826	38.0	35.0	40.0	34.0	41.0
23	36.87386996632935	38.0	35.0	40.0	33.0	41.0
24	36.67335090682688	37.0	35.0	40.0	33.0	41.0
25	36.51026053773261	37.0	35.0	40.0	33.0	41.0
26	36.66314585897831	37.0	35.0	40.0	33.0	41.0
27	36.692966835376275	38.0	35.0	40.0	33.0	41.0
28	36.63384720020445	38.0	35.0	40.0	33.0	41.0
29	36.501570516442825	38.0	35.0	40.0	33.0	41.0
30	36.21954648983211	38.0	35.0	40.0	32.0	41.0
31	35.74645093828812	38.0	35.0	40.0	30.0	41.0
32	35.05193954962577	38.0	35.0	40.0	24.0	41.0
33	34.241523538401545	38.0	34.0	40.0	18.0	41.0
34	33.543952569894344	38.0	34.0	40.0	15.0	41.0
35	33.09576841271441	38.0	33.0	40.0	10.0	41.0
36	32.91342865375432	38.0	33.0	40.0	10.0	41.0
37	32.67986409560143	37.0	33.0	40.0	10.0	41.0
38	32.53596813557044	37.0	32.0	40.0	10.0	41.0
39	32.47686479100677	37.0	32.0	40.0	9.0	41.0
40	32.34254974489926	37.0	31.0	40.0	8.0	41.0
41	32.21157732795336	37.0	31.0	40.0	8.0	41.0
42	32.079021667527016	37.0	31.0	40.0	8.0	41.0
43	31.982131239175654	37.0	31.0	40.0	8.0	41.0
44	31.873288595250678	36.0	30.0	40.0	8.0	41.0
45	31.834290915644175	36.0	30.0	40.0	8.0	41.0
46	31.802637245828333	36.0	30.0	40.0	8.0	41.0
47	31.79177650100136	36.0	30.0	40.0	8.0	41.0
48	31.749911674358888	36.0	30.0	40.0	8.0	41.0
49	31.64728695193558	36.0	29.0	40.0	8.0	41.0
50	31.550705026976228	35.0	29.0	40.0	8.0	41.0
51	30.989873350703196	35.0	27.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	10.0
11	7.0
12	15.0
13	23.0
14	25.0
15	44.0
16	81.0
17	143.0
18	325.0
19	611.0
20	1109.0
21	1797.0
22	2926.0
23	4999.0
24	9374.0
25	19559.0
26	35050.0
27	46016.0
28	43055.0
29	34779.0
30	27599.0
31	24949.0
32	24933.0
33	30991.0
34	56099.0
35	74831.0
36	71701.0
37	91575.0
38	163759.0
39	215592.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.46632782201696	15.315004362828496	17.47686988182182	35.74179793333273
2	49.070060814876584	19.109290635649348	17.806143799234544	14.014504750239523
3	23.5427796461069	19.17689665950898	44.08951281918137	13.190810875202741
4	22.176710335678163	19.13739193472353	44.10804338596218	14.577854343636124
5	19.798586993374816	22.174368560755315	42.72293442724767	15.304110018622202
6	20.21389568512698	26.20985765062958	41.547872497482594	12.028374166760846
7	59.991182708333966	4.720305530356021	31.254855364853622	4.033656396456385
8	59.95992510392899	5.030437983181983	30.579507840364258	4.430129072524769
9	55.192682258815	6.329410351256056	32.21070679857987	6.267200591349075
10	31.666702302372013	19.704915996460866	37.66643147101137	10.961950230155749
11	24.591792995242123	18.273989702299318	42.26852827591403	14.86568902654453
12	22.174063111852334	16.52885830327207	45.98930317941763	15.307775405457965
13	19.007677967257912	19.182700188665606	46.50154098971554	15.308080854360945
14	15.180912294420162	21.72281326584949	45.92994427593847	17.166330163791883
15	14.488663263965886	19.42237576120412	50.594658105952085	15.494302868877913
16	15.437285740321597	18.813921546467434	48.11410756484935	17.634685148361623
17	15.511102558541829	19.009307028073806	45.62317176104529	19.856418652339077
18	16.55268331770453	18.978660321474788	46.747020091410675	17.721636269410006
19	17.845037626214033	20.3791435416393	44.680454630147196	17.09536420199947
20	18.990878277594	20.18976522179154	45.71857363507612	15.10078286553834
21	18.11759986397342	20.817869982619957	45.36842737595975	15.696102777446875
22	16.828605493396704	19.094629088306295	44.60633236302399	19.470433055273016
23	16.24611443541334	19.921173819770893	45.237186163979224	18.595525580836544
24	17.174373651570363	18.20444916872081	46.07767972867992	18.543497451028905
25	15.74772364205054	20.667487306052674	44.91453030613107	18.670258745765715
26	15.245158380347009	20.64600406654306	44.87013839889794	19.23869915421199
27	15.859110675337343	20.69202503459209	45.43633884872236	18.01252544134821
28	13.831540857354344	20.159322147794505	47.03699291663994	18.97214407821121
29	15.598766393697163	19.063371483901314	45.22761543168584	20.110246690715677
30	15.812478809482355	20.32395910650087	45.0440406409947	18.819521443022072
31	16.369006710712398	21.593812012490822	41.87256468135062	20.164616595446162
32	17.210314805821042	23.192531570689532	40.58173761735601	19.015416006133414
33	17.073880962489856	22.291660939499735	39.07791085168318	21.55654724632723
34	16.08514286354274	23.120649262188174	39.11700831126465	21.677199563004436
35	16.47051756280284	24.355273728034405	36.88326048376997	22.290948225392782
36	17.49387320408772	24.565015308080852	35.908776666961934	22.03233482086949
37	17.197282319293883	26.30057597481472	37.03506858855116	19.467073117340234
38	16.676593756013524	27.60270464821959	33.25585112827734	22.464850467489548
39	17.823961651908395	25.612094147497206	34.72465308640844	21.83929111418596
40	17.965995391794216	24.962404330858178	36.11128928963785	20.960310987709754
41	15.963676016457587	26.160578560948768	34.61377513462661	23.261970287967042
42	16.795006114068876	25.64436991491212	34.37633952070994	23.184284450309065
43	18.23234683519301	24.269849851500926	34.60583346314912	22.891969850156947
44	17.076731818917672	24.694831091847465	34.27859587175626	23.9498412174786
45	17.044354235201766	24.69554380595442	34.09318838764724	24.166913571196574
46	18.11668351726448	24.98653479419362	34.90975512161448	21.98702656692742
47	16.06111421650829	24.452202846580143	38.43249731968588	21.05418561722569
48	16.166799536939465	23.08755896436531	37.27973315983836	23.465908338856867
49	16.690033507744655	22.11562055508211	38.53838627271903	22.655959664454198
50	16.291219056753427	22.036712921812207	38.13743367940694	23.534634342027427
51	15.672277763014414	22.581531948428008	35.91722741994439	25.828962868613193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	291.0
1	672.5
2	1054.0
3	38494.0
4	75934.0
5	52524.5
6	29115.0
7	27149.0
8	25183.0
9	24038.0
10	22893.0
11	21842.0
12	20791.0
13	19187.0
14	17583.0
15	15916.5
16	14250.0
17	12892.0
18	11534.0
19	10475.5
20	9417.0
21	8590.0
22	7763.0
23	7739.0
24	7715.0
25	7937.0
26	8168.0
27	8177.0
28	9061.0
29	9945.0
30	11824.0
31	13703.0
32	15117.0
33	16531.0
34	16933.5
35	17336.0
36	19585.0
37	21834.0
38	22649.5
39	23465.0
40	27017.5
41	30570.0
42	36257.0
43	41944.0
44	46817.0
45	51690.0
46	63380.5
47	75071.0
48	76141.5
49	77212.0
50	75266.5
51	73321.0
52	64239.0
53	55157.0
54	48072.5
55	40988.0
56	37659.0
57	34330.0
58	32437.0
59	30544.0
60	29454.5
61	28365.0
62	26206.5
63	24048.0
64	20911.0
65	17774.0
66	14989.5
67	12205.0
68	10096.0
69	7987.0
70	7455.5
71	6924.0
72	5844.0
73	4764.0
74	3665.0
75	2130.5
76	1695.0
77	1364.0
78	1033.0
79	913.5
80	794.0
81	507.0
82	220.0
83	170.0
84	120.0
85	113.0
86	106.0
87	81.0
88	56.0
89	29.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	982161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.59172001195604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03347721274747	24.02028323640558
2	10.18723347735126	6.436644554258127
3	4.141575560146552	3.9251848651352965
4	2.029322341525359	2.564391329099047
5	1.200373665671043	1.8960934377802459
6	0.7647913519027484	1.4496644554126181
7	0.5458773277780388	1.2071642590026987
8	0.4035734737999912	1.0199664150833438
9	0.29607633762326396	0.8418204684323569
>10	3.4552664115206806	27.899779175094437
>50	0.8514098810351425	17.165609764189142
>100	0.08182208218680809	4.420698588509141
>500	0.006572054794120409	1.4638425417190313
>1k	0.002300219177942143	1.5957866499755298
>5k	0.0	0.0
>10k+	3.2860273970602044E-4	4.093070259903408
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39428	4.014413115568629	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3520	0.3583933794968442	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	2827	0.28783468290840303	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	2371	0.2414064496554027	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	2239	0.2279666979242711	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	1770	0.1802148527583563	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1592	0.16209155118152727	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1053	0.107212564946073	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	998	0.10161266839143482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17227318128086944	0.0
2	0.0	0.0	0.0	1.0568532043117167	0.0
3	0.0	0.0	0.0	1.4816308120562718	0.0
4	0.0	0.0	0.0	1.9534475508597877	0.0
5	0.0	0.0	0.0	3.3234876970272693	0.0
6	0.0	0.0	0.0	3.9378472572215757	0.0
7	0.0	0.0	0.0	4.639463387367244	0.0
8	0.0	0.0	0.0	5.669437088216697	0.0
9	0.0	0.0	0.0	6.023859631974799	0.0
10	0.0	0.0	0.0	7.085090937229232	0.0
11	0.0	0.0	0.0	8.334580583020502	0.0
12	0.0	0.0	0.0	9.550267216881958	0.0
13	1.0181630099342165E-4	0.0	0.0	9.945619913639414	0.0
14	1.0181630099342165E-4	0.0	0.0	10.095289876099743	0.0
15	1.0181630099342165E-4	0.0	0.0	10.44543613521612	0.0
16	1.0181630099342165E-4	0.0	0.0	11.108565703586276	0.0
17	1.0181630099342165E-4	0.0	0.0	11.895809342867413	0.0
18	1.0181630099342165E-4	0.0	0.0	12.778658488781371	0.0
19	1.0181630099342165E-4	0.0	0.0	13.328059248941875	0.0
20	1.0181630099342165E-4	0.0	0.0	13.834289897481167	0.0
21	1.0181630099342165E-4	0.0	0.0	14.432562482118513	0.0
22	1.0181630099342165E-4	0.0	0.0	15.096302948294628	0.0
23	1.0181630099342165E-4	0.0	0.0	15.731331217590599	0.0
24	1.0181630099342165E-4	0.0	0.0	16.257416044823607	0.0
25	1.0181630099342165E-4	0.0	0.0	16.729843681433085	0.0
26	2.036326019868433E-4	0.0	0.0	17.14118153744651	0.0
27	2.036326019868433E-4	0.0	0.0	17.556184780295695	0.0
28	2.036326019868433E-4	0.0	0.0	17.96721718740614	0.0
29	2.036326019868433E-4	0.0	0.0	18.400140099230168	0.0
30	2.036326019868433E-4	0.0	0.0	18.88376752894892	0.0
31	2.036326019868433E-4	0.0	0.0	19.325141193755403	0.0
32	2.036326019868433E-4	0.0	0.0	19.788507179576463	0.0
33	2.036326019868433E-4	0.0	0.0	20.236091638743545	0.0
34	2.036326019868433E-4	0.0	0.0	20.655880247739425	0.0
35	2.036326019868433E-4	0.0	0.0	21.117413540142604	0.0
36	2.036326019868433E-4	0.0	0.0	21.543922025004047	0.0
37	2.036326019868433E-4	0.0	0.0	21.979186711750923	0.0
38	3.0544890298026494E-4	0.0	0.0	22.389302772152426	0.0
39	3.0544890298026494E-4	0.0	0.0	22.80481509650658	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	35	1.2116834E-7	45.000004	1
CGGGTAC	40	6.8139343E-9	45.0	6
CGAACCG	20	7.032784E-4	45.0	1
ATTAGCG	45	3.8380676E-10	45.0	1
CTATCGT	85	0.0	45.0	31
ACCGCAA	20	7.032784E-4	45.0	33
ACATTCG	20	7.032784E-4	45.0	29
CGCGAAT	20	7.032784E-4	45.0	32
CCGTAGA	20	7.032784E-4	45.0	25
CCGTAAG	20	7.032784E-4	45.0	43
TACGACA	20	7.032784E-4	45.0	17
ACGCTAG	25	3.8903898E-5	45.0	1
TCGTAGC	20	7.032784E-4	45.0	28
CTATTAC	25	3.8903898E-5	45.0	37
TGTAGCG	20	7.032784E-4	45.0	1
CGCACTC	20	7.032784E-4	45.0	44
AATCGAC	25	3.8903898E-5	45.0	24
TCTATCG	85	0.0	45.0	30
CGTTTTT	23950	0.0	44.351772	1
ATCGTGT	90	0.0	42.5	33
>>END_MODULE