FastQCFastQC Report
Sat 18 Jun 2016
SRR3549890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365578
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT505793.70385287402111No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC29820.21836907155797763No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC25170.18431755637539562No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT15630.11445702845242088Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA13670.10010413173030029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG351.2121563E-745.0000041
ATTGTCG207.033753E-445.013
CTAAGCG253.891193E-545.01
TCGATCC253.891193E-545.013
TAGCCGT207.033753E-445.044
ATCGTGT207.033753E-445.030
CCGTAGA207.033753E-445.032
CAAATCG207.033753E-445.014
CGTAACG406.8175723E-945.01
ACGTATC207.033753E-445.037
CGTTTTT379400.044.5077741
AATACGG800.042.18752
GTACACG501.0822987E-940.51
TACGAAT950.040.2631612
GTTTTTT420950.040.248252
ATTACGG900.040.02
CGAATAT900.040.014
TTACGAG451.9286745E-840.01
TCGGCGT1550.039.1935464
ATGGGCG4250.039.1764685