##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1365578
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.617320284890354	34.0	31.0	34.0	31.0	34.0
2	32.77232351429212	34.0	31.0	34.0	31.0	34.0
3	32.81283310070901	34.0	31.0	34.0	31.0	34.0
4	36.20951787448245	37.0	37.0	37.0	35.0	37.0
5	36.104547671388964	37.0	35.0	37.0	35.0	37.0
6	36.09936964420927	37.0	36.0	37.0	35.0	37.0
7	36.24538547047477	37.0	37.0	37.0	35.0	37.0
8	36.24291911556865	37.0	37.0	37.0	35.0	37.0
9	38.01656148531977	39.0	39.0	39.0	35.0	39.0
10	37.60053032488807	39.0	38.0	39.0	35.0	39.0
11	37.510867193232464	39.0	37.0	39.0	35.0	39.0
12	37.447862370366245	39.0	37.0	39.0	35.0	39.0
13	37.42485745962515	39.0	37.0	39.0	35.0	39.0
14	38.73581223481925	40.0	38.0	41.0	35.0	41.0
15	38.77378296955575	40.0	38.0	41.0	35.0	41.0
16	38.862192419620115	40.0	38.0	41.0	35.0	41.0
17	38.869450152243225	40.0	38.0	41.0	35.0	41.0
18	38.35676101987583	39.0	38.0	41.0	35.0	41.0
19	37.859739245945676	38.0	37.0	41.0	35.0	41.0
20	37.28724100710468	38.0	35.0	41.0	34.0	41.0
21	37.229954641917196	38.0	35.0	41.0	34.0	41.0
22	37.05062178799014	38.0	35.0	41.0	34.0	41.0
23	36.943965851822455	38.0	35.0	40.0	33.0	41.0
24	36.72813343507291	37.0	35.0	40.0	33.0	41.0
25	36.57912986295913	37.0	35.0	40.0	33.0	41.0
26	36.69440998610112	37.0	35.0	40.0	33.0	41.0
27	36.71478817028394	38.0	35.0	40.0	33.0	41.0
28	36.62632013696764	38.0	35.0	40.0	33.0	41.0
29	36.47280711903677	38.0	35.0	40.0	33.0	41.0
30	36.12693086736898	38.0	35.0	40.0	32.0	41.0
31	35.553604407803874	38.0	35.0	40.0	30.0	41.0
32	34.77203865322962	38.0	35.0	40.0	21.0	41.0
33	33.809171647463565	38.0	34.0	40.0	15.0	41.0
34	33.014109043935974	38.0	33.0	41.0	10.0	41.0
35	32.531514860374145	38.0	32.0	41.0	9.0	41.0
36	32.29909898958536	38.0	31.0	41.0	8.0	41.0
37	32.080956195837956	37.0	31.0	40.0	8.0	41.0
38	31.926292749297367	37.0	30.0	40.0	7.0	41.0
39	31.864191573092125	37.0	30.0	40.0	7.0	41.0
40	31.74635136184092	37.0	29.0	40.0	7.0	41.0
41	31.6094576801911	37.0	29.0	40.0	7.0	41.0
42	31.48375779340323	37.0	27.0	40.0	7.0	41.0
43	31.355289115671166	36.0	27.0	40.0	7.0	41.0
44	31.252092520529768	36.0	26.0	40.0	7.0	41.0
45	31.201900587150643	36.0	25.0	40.0	7.0	41.0
46	31.13614235144386	36.0	25.0	40.0	7.0	41.0
47	31.116060012683274	35.0	25.0	40.0	7.0	41.0
48	31.048242575671253	35.0	25.0	40.0	7.0	41.0
49	30.91115044325553	35.0	24.0	40.0	7.0	41.0
50	30.818583046885642	35.0	24.0	40.0	7.0	41.0
51	30.285506942847643	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	9.0
11	18.0
12	20.0
13	30.0
14	49.0
15	69.0
16	126.0
17	268.0
18	551.0
19	1007.0
20	1626.0
21	2701.0
22	4325.0
23	7664.0
24	14963.0
25	32717.0
26	57767.0
27	72693.0
28	66675.0
29	51387.0
30	38961.0
31	33806.0
32	33252.0
33	39481.0
34	69260.0
35	87113.0
36	93317.0
37	119928.0
38	222433.0
39	313110.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.784107535417238	14.239977504031259	17.10623633362576	37.86967862692574
2	52.105628532387016	17.118099442140984	18.02116027059604	12.755111754875958
3	23.211123787875902	17.712572991070445	45.96266196438431	13.113641256669336
4	21.130612824752596	19.047538844357483	45.99327171351618	13.828576617373741
5	19.401601373191426	21.910502366031086	44.27758795176841	14.410308309009078
6	20.166698643358345	25.0671876670538	43.70625478735012	11.059858902237734
7	59.187684628779905	4.245967641540798	32.91954029722213	3.646807432457172
8	59.66513813198514	4.05762248659542	32.53003490097234	3.7472044804471074
9	55.29394878945033	5.604879399053002	34.00582024607895	5.095351565417721
10	29.764905410016855	20.131695150331947	39.05240125426742	11.050998185383772
11	23.54717196674229	18.71676315816453	43.18252051512253	14.55354435997065
12	22.114372082737127	15.99952547565939	47.21400022554552	14.672102216057961
13	18.80427189073052	17.72033527195078	47.61200019332473	15.86339264399397
14	15.05655480682905	20.56997110381099	47.408423392878326	16.965050696481637
15	14.32755946566216	19.109783549529944	51.523530695427134	15.03912628938076
16	16.0379707347365	17.604999494719454	50.24663549061277	16.11039427993128
17	15.545578502289873	17.88099984036064	47.94255619232296	18.63086546502653
18	15.980925293172563	18.539255904825648	48.58433571718349	16.895483084818295
19	18.035293480123435	19.601223804132754	46.351142153725384	16.012340562018426
20	20.30598032481484	18.551411929600505	46.21083526536016	14.931772480224492
21	18.560419104584287	19.044243536436586	46.44194619421227	15.953391164766861
22	17.166503854045686	18.433952509486826	46.24386157363402	18.155682062833467
23	17.31193677695452	19.16206910187481	46.548714170849266	16.977279950321403
24	17.30234376945147	17.24302822687536	47.96437845366577	17.4902495500074
25	17.009647197011084	18.900568111085562	46.18059166155284	17.90919303035052
26	17.055781507903614	20.032396538315645	45.96808091518756	16.94374103859318
27	17.054390155670347	19.789422500948316	46.49174195835023	16.6644453850311
28	14.996506973603852	20.084389174400876	47.50823460834899	17.410869243646278
29	17.001958145195662	18.678610815347056	46.711648840271295	17.607782199185987
30	16.67521005757269	18.946482734783366	45.833632352015044	18.544674855628898
31	17.182248103001076	21.166128921233355	43.90250868130565	17.74911429445993
32	18.757771434513444	21.481965878184912	42.53048892117477	17.229773766126872
33	17.92120259699556	22.84373356922856	40.48944842403729	18.745615409738587
34	18.02892255147637	22.952991334072458	40.348628932217714	18.669457182233458
35	18.813864898233568	23.80603671119482	36.77285369272206	20.607244697849556
36	18.500957103878356	25.95355226870966	36.1550933011516	19.39039732626038
37	19.144860271621248	25.98284389467317	37.152107019884625	17.72018881382096
38	18.423700440399596	25.797794047648686	35.848117061054005	19.930388450897716
39	18.937695246994313	24.737949791223937	36.72254532512972	19.601809636652025
40	19.796306033049742	24.417352945053302	37.19758226919297	18.58875875270398
41	18.079597064393248	25.845319710774483	35.971947409814746	20.103135815017524
42	19.15247609437176	25.476611368958785	35.327385180487674	20.043527356181777
43	18.994374543233707	25.887939026551393	35.246613521893295	19.871072908321604
44	18.850552659752868	25.71870665754721	34.813902977347325	20.616837705352605
45	18.50256814330635	24.495634815440788	34.511027564884614	22.49076947636825
46	18.234476536675313	25.23964211491398	35.46703300726872	21.058848341141992
47	17.5086300452995	24.36462801831898	38.011669783783866	20.115072152597655
48	17.94932255792053	22.9349769841049	37.737280477570664	21.3784199804039
49	17.782140602733786	22.9091271241921	38.50032733392014	20.80840493915397
50	16.83235963086693	22.66439558926696	38.22593802770695	22.27730675215916
51	16.504659565400146	22.694419505879562	36.95526729340982	23.84565363531047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	409.0
1	803.5
2	1198.0
3	53961.0
4	106724.0
5	75521.5
6	44319.0
7	42369.0
8	40419.0
9	38600.0
10	36781.0
11	34574.5
12	32368.0
13	30133.0
14	27898.0
15	25626.5
16	23355.0
17	21304.0
18	19253.0
19	17683.5
20	16114.0
21	15068.5
22	14023.0
23	13210.5
24	12398.0
25	12376.0
26	13125.0
27	13896.0
28	13834.5
29	13773.0
30	15591.0
31	17409.0
32	19334.5
33	21260.0
34	23654.0
35	26048.0
36	28930.5
37	31813.0
38	32811.0
39	33809.0
40	38472.5
41	43136.0
42	47503.5
43	51871.0
44	56742.5
45	61614.0
46	70346.0
47	79078.0
48	84003.5
49	88929.0
50	88944.0
51	88959.0
52	80244.0
53	71529.0
54	63981.5
55	56434.0
56	53719.0
57	51004.0
58	51167.0
59	51330.0
60	50281.5
61	49233.0
62	45955.0
63	42677.0
64	36128.0
65	29579.0
66	23554.0
67	17529.0
68	14927.5
69	12326.0
70	10573.0
71	8820.0
72	7422.5
73	6025.0
74	5196.5
75	3377.0
76	2386.0
77	1950.5
78	1515.0
79	1096.0
80	677.0
81	575.5
82	474.0
83	368.0
84	262.0
85	208.0
86	154.0
87	91.0
88	28.0
89	21.0
90	14.0
91	8.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1365578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.735741571298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07201594264706	25.557453378487516
2	9.433538331414825	6.176277458402589
3	3.435614581414417	3.3740217322729653
4	1.6613181958922911	2.175379325137003
5	0.9835775114900198	1.6099069615738841
6	0.6525494444743107	1.2817013986083068
7	0.41899893332708005	0.9601368560031369
8	0.33867991157462585	0.8869550448557608
9	0.25720055088874416	0.7577685689300468
>10	4.035884710243207	34.300713994185706
>50	0.6224559550533977	12.529298424758451
>100	0.0822426390557574	4.690820932506192
>500	0.0034172841486358367	0.8144687461928833
>1k	0.002278189432423891	1.1130053628955763
>5k	0.0	0.0
>10k+	2.278189432423891E-4	3.7720918151899867
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50579	3.70385287402111	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2982	0.21836907155797763	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	2517	0.18431755637539562	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	1563	0.11445702845242088	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1367	0.10010413173030029	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6614532454389275E-4	0.0	0.0	0.12170670587838996	0.0
2	3.6614532454389275E-4	0.0	0.0	0.6813964489761845	0.0
3	3.6614532454389275E-4	0.0	0.0	0.979804888479457	0.0
4	3.6614532454389275E-4	0.0	0.0	1.3129971338143995	0.0
5	3.6614532454389275E-4	0.0	0.0	2.1733654174276387	0.0
6	3.6614532454389275E-4	0.0	0.0	2.591210461797129	0.0
7	3.6614532454389275E-4	0.0	0.0	3.0635379304587507	0.0
8	3.6614532454389275E-4	0.0	0.0	3.7515249952767253	0.0
9	3.6614532454389275E-4	0.0	0.0	3.9982337149544	0.0
10	3.6614532454389275E-4	0.0	0.0	4.665204038143555	0.0
11	3.6614532454389275E-4	0.0	0.0	5.537435430272017	0.0
12	5.126034543614499E-4	0.0	0.0	6.3189360109785015	0.0
13	5.126034543614499E-4	0.0	0.0	6.591348132439157	0.0
14	5.126034543614499E-4	0.0	0.0	6.708294949098477	0.0
15	5.126034543614499E-4	0.0	0.0	6.946802013506368	0.0
16	5.126034543614499E-4	0.0	0.0	7.427990199021953	0.0
17	5.126034543614499E-4	0.0	0.0	7.998371385596429	0.0
18	5.126034543614499E-4	0.0	0.0	8.6487919401162	0.0
19	5.858325192702285E-4	0.0	0.0	9.040128062988712	0.0
20	6.59061584179007E-4	0.0	0.0	9.424434195629983	0.0
21	6.59061584179007E-4	0.0	0.0	9.888926154346365	0.0
22	7.322906490877855E-4	0.0	0.0	10.435068520436035	0.0
23	7.322906490877855E-4	0.0	0.0	10.97132496276302	0.0
24	7.322906490877855E-4	0.0	0.0	11.402058322556456	0.0
25	8.055197139965641E-4	0.0	0.0	11.785851851743365	0.0
26	8.787487789053426E-4	0.0	0.0	12.126367003569184	0.0
27	9.519778438141212E-4	0.0	0.0	12.451137906439618	0.0
28	9.519778438141212E-4	0.0	0.0	12.786453794656914	0.0
29	0.0010252069087228997	0.0	0.0	13.140369865361041	0.0
30	0.0010252069087228997	0.0	0.0	13.537710771556073	0.0
31	0.0010252069087228997	0.0	0.0	13.91798930562736	0.0
32	0.0010252069087228997	0.0	0.0	14.295558364297023	0.0
33	0.0010984359736316784	0.0	0.0	14.644861003911897	0.0
34	0.0010984359736316784	0.0	0.0	14.987060424230618	0.0
35	0.0010984359736316784	0.0	0.0	15.364556253835373	0.0
36	0.0010984359736316784	0.0	0.0	15.748789157411734	0.0
37	0.001171665038540457	0.0	0.0	16.131264563430285	0.0
38	0.001171665038540457	0.0	0.0	16.483569594706417	0.0
39	0.0012448941034492354	0.0	0.0	16.847591276367954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2121563E-7	45.000004	1
ATTGTCG	20	7.033753E-4	45.0	13
CTAAGCG	25	3.891193E-5	45.0	1
TCGATCC	25	3.891193E-5	45.0	13
TAGCCGT	20	7.033753E-4	45.0	44
ATCGTGT	20	7.033753E-4	45.0	30
CCGTAGA	20	7.033753E-4	45.0	32
CAAATCG	20	7.033753E-4	45.0	14
CGTAACG	40	6.8175723E-9	45.0	1
ACGTATC	20	7.033753E-4	45.0	37
CGTTTTT	37940	0.0	44.507774	1
AATACGG	80	0.0	42.1875	2
GTACACG	50	1.0822987E-9	40.5	1
TACGAAT	95	0.0	40.26316	12
GTTTTTT	42095	0.0	40.24825	2
ATTACGG	90	0.0	40.0	2
CGAATAT	90	0.0	40.0	14
TTACGAG	45	1.9286745E-8	40.0	1
TCGGCGT	155	0.0	39.193546	4
ATGGGCG	425	0.0	39.176468	5
>>END_MODULE