##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549889_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 776567 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.368314646385954 33.0 31.0 34.0 30.0 34.0 2 32.54428787213467 34.0 31.0 34.0 31.0 34.0 3 32.619677374907766 34.0 31.0 34.0 31.0 34.0 4 36.068263266402 37.0 35.0 37.0 35.0 37.0 5 35.92612227921094 37.0 35.0 37.0 35.0 37.0 6 35.91166505916424 37.0 35.0 37.0 35.0 37.0 7 36.12357079299017 37.0 35.0 37.0 35.0 37.0 8 36.11386783110794 37.0 36.0 37.0 35.0 37.0 9 37.90509898051295 39.0 38.0 39.0 35.0 39.0 10 37.49577950131798 39.0 37.0 39.0 35.0 39.0 11 37.33154898418295 39.0 37.0 39.0 34.0 39.0 12 37.07671199007942 39.0 35.0 39.0 34.0 39.0 13 36.89978327690979 39.0 35.0 39.0 33.0 39.0 14 38.01985533765921 40.0 36.0 41.0 33.0 41.0 15 38.048373160332595 40.0 36.0 41.0 33.0 41.0 16 38.14661452263616 40.0 36.0 41.0 34.0 41.0 17 38.12741463389508 40.0 36.0 41.0 33.0 41.0 18 37.69848963450674 39.0 36.0 40.0 33.0 41.0 19 37.26100645533483 37.0 36.0 40.0 34.0 41.0 20 36.75198920376477 37.0 35.0 40.0 33.0 41.0 21 36.70882229092918 37.0 35.0 40.0 33.0 41.0 22 36.529467515359265 36.0 35.0 40.0 33.0 41.0 23 36.41692088383874 36.0 35.0 40.0 33.0 41.0 24 36.21027548170345 36.0 35.0 40.0 32.0 41.0 25 36.11254920695832 35.0 35.0 40.0 32.0 41.0 26 36.27681706794134 36.0 35.0 40.0 33.0 41.0 27 36.34521168167074 36.0 35.0 40.0 33.0 41.0 28 36.301614670723836 36.0 35.0 40.0 33.0 41.0 29 36.22577961721268 36.0 35.0 40.0 33.0 41.0 30 35.93020692354942 36.0 35.0 40.0 32.0 41.0 31 35.39564776767491 36.0 35.0 40.0 30.0 41.0 32 34.69849736082012 36.0 35.0 40.0 23.0 41.0 33 33.83559177765731 36.0 34.0 40.0 18.0 41.0 34 33.108012573287304 36.0 33.0 40.0 12.0 41.0 35 32.69032935986206 36.0 33.0 40.0 10.0 41.0 36 32.530191213378885 36.0 32.0 40.0 10.0 41.0 37 32.34742269501537 36.0 32.0 40.0 10.0 41.0 38 32.272392465814285 36.0 31.0 40.0 10.0 41.0 39 32.277212397642444 36.0 31.0 40.0 10.0 41.0 40 32.16463228543062 36.0 31.0 40.0 9.0 41.0 41 32.03349871936356 36.0 31.0 40.0 8.0 41.0 42 31.882857499739238 35.0 31.0 40.0 8.0 41.0 43 31.75428134339986 35.0 30.0 40.0 8.0 41.0 44 31.627138418191862 35.0 30.0 40.0 8.0 41.0 45 31.629183315798894 35.0 30.0 40.0 8.0 41.0 46 31.57860944387284 35.0 30.0 40.0 8.0 41.0 47 31.515421077640436 35.0 30.0 40.0 8.0 41.0 48 31.46415827610496 35.0 29.0 40.0 8.0 41.0 49 31.35025696430572 35.0 29.0 40.0 7.0 41.0 50 31.21151426728151 35.0 28.0 40.0 7.0 41.0 51 30.62122134986421 35.0 27.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 8.0 9 7.0 10 7.0 11 7.0 12 10.0 13 16.0 14 22.0 15 38.0 16 69.0 17 159.0 18 338.0 19 615.0 20 1009.0 21 1769.0 22 2738.0 23 4506.0 24 8731.0 25 17318.0 26 28902.0 27 34660.0 28 31835.0 29 27073.0 30 23693.0 31 21662.0 32 22757.0 33 29669.0 34 59667.0 35 70852.0 36 53278.0 37 70111.0 38 128724.0 39 136255.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.735403384382803 15.477222184306056 18.739142920057123 35.048231511254016 2 48.0281804403226 19.122625607320423 19.003254065650484 13.845939886706491 3 23.153829611611105 19.14181261887255 44.247566533216066 13.45679123630028 4 22.29492110790183 19.272902402497145 44.18987672667007 14.242299762930951 5 18.846667447882798 22.28294532216795 43.596367087450275 15.274020142498973 6 19.894484313652267 25.049223054804028 42.03809845126049 13.01819418028322 7 59.130635218854266 4.404384940385054 31.571132947961996 4.893846892798689 8 58.73594937719475 4.063396976693576 31.221388495776925 5.979265150334743 9 55.14836453261599 4.757091146031186 33.30569030103005 6.78885402032278 10 35.473822606420306 15.360168536649125 37.74239698570761 11.42361187122296 11 27.50889491827492 16.244702646391104 40.91945704620464 15.326945389129335 12 24.524735148416042 15.104041248211681 45.56863734874132 14.80258625463096 13 20.149452655083206 19.45434199495987 45.85090533076991 14.54530001918701 14 16.164477759163084 21.74751180516298 45.47386123798719 16.614149197686743 15 14.980291462294948 18.403177060060496 52.855709809971316 13.760821667673234 16 17.21886199130275 18.537099825256544 47.32856276406285 16.915475419377852 17 15.70759509482118 19.575258799305146 44.73355164461019 19.983594461263486 18 17.921570192913165 18.95264671303313 47.56653321606507 15.559249877988634 19 17.715663941424243 20.673554245802357 44.964697186462985 16.646084626310415 20 19.799321887229304 20.994968882272875 45.25366130675138 13.952047923746438 21 18.55345385523722 22.103694851828624 44.98143753211249 14.361413760821668 22 16.61337656634907 20.22787473585666 43.93297680689496 19.225771890899303 23 16.31192157276835 19.418929725316683 44.989678933047635 19.279469768867337 24 18.442194942612808 17.068456424236413 46.02912562599235 18.46022300715843 25 14.910883413794302 19.140653671866044 43.89370138056343 22.054761533776222 26 12.974798053484115 20.909979435129227 45.375221970544715 20.740000540841937 27 15.148596322017289 20.346731189968153 46.12827998099327 18.37639250702129 28 11.742837385570079 19.974709200880284 48.438061364956276 19.84439204859336 29 14.556116857914384 18.00771858706332 47.16283334213275 20.273331212889552 30 16.265306148728957 18.75034607445333 45.72754186052202 19.256805916295697 31 17.13554657872405 19.58118230622728 44.88254072089079 18.400730394157875 32 19.79790539644358 19.44725954103123 42.59465055816175 18.160184504363436 33 19.34218167910818 20.436098881358593 40.60718521389654 19.61453422563668 34 16.19654195967637 20.58032339772357 42.873828014839674 20.349306627760384 35 17.255433207952436 22.166406762069467 39.011186414050556 21.566973615927537 36 19.57822055276621 23.875982368552876 38.12330423517868 18.422492843502237 37 18.5488180672112 23.838509748675904 40.85623004840535 16.756442135707545 38 18.109062064187636 25.33355138706641 36.34045742350628 20.216929125239677 39 20.10000424947236 22.56869014521606 37.193571192183036 20.137734413128552 40 20.86065980140799 20.582126204178135 36.32564865620095 22.23156533821293 41 19.11039227780732 22.00840365351605 34.50597308409964 24.37523098457699 42 20.497265528924096 20.890277336018656 35.13193323950155 23.480523895555695 43 20.986856253227344 20.14108248225845 36.07145294610768 22.800608318406525 44 17.216930412958572 21.633162367187893 36.7230387075423 24.426868512311238 45 17.557532060981217 20.543237093515433 35.9495059666455 25.949724878857843 46 19.940713422022828 21.23744635041149 36.83519902339399 21.986641204171693 47 16.14606337894863 20.412533625559675 40.700544833864946 22.74085816162675 48 17.938439310452285 18.672567853127934 39.83558405134392 23.553408785075852 49 19.273675033834813 17.225171813893713 41.78119853148537 21.7199546207861 50 18.195854317785845 17.911397213633855 39.65556094966693 24.237187518913373 51 16.671195144784672 18.42056126515806 36.841895161653795 28.066348428403476 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 642.0 1 766.5 2 891.0 3 28555.5 4 56220.0 5 37102.5 6 17985.0 7 17452.0 8 16919.0 9 17062.0 10 17205.0 11 16865.0 12 16525.0 13 15537.0 14 14549.0 15 13425.0 16 12301.0 17 11052.0 18 9803.0 19 9077.5 20 8352.0 21 7644.5 22 6937.0 23 6563.5 24 6190.0 25 5779.5 26 5641.0 27 5913.0 28 6277.5 29 6642.0 30 7718.5 31 8795.0 32 9037.5 33 9280.0 34 9347.5 35 9415.0 36 10422.0 37 11429.0 38 13108.0 39 14787.0 40 18167.5 41 21548.0 42 25529.0 43 29510.0 44 36480.5 45 43451.0 46 52283.5 47 61116.0 48 68928.5 49 76741.0 50 76278.0 51 75815.0 52 63409.5 53 51004.0 54 44007.5 55 37011.0 56 32671.0 57 28331.0 58 26667.0 59 25003.0 60 23407.0 61 21811.0 62 20619.5 63 19428.0 64 16080.0 65 12732.0 66 9872.0 67 7012.0 68 5372.0 69 3732.0 70 2980.0 71 2228.0 72 1806.5 73 1385.0 74 1329.0 75 922.5 76 572.0 77 437.5 78 303.0 79 245.0 80 187.0 81 145.5 82 104.0 83 76.0 84 48.0 85 30.0 86 12.0 87 32.5 88 53.0 89 27.5 90 2.0 91 3.0 92 4.0 93 2.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 776567.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.718931752800074 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0020343719397 24.2126751218386 2 10.831439718886939 7.087862738936594 3 4.66187613151268 4.5759482096091295 4 2.4995549348754564 3.2713106930625866 5 1.49400338230859 2.4441097352103616 6 0.9667975975910424 1.8979550768611428 7 0.7132746762901764 1.633630981817719 8 0.5044517005312877 1.3204096609813711 9 0.36352900599066623 1.0704852663454671 >10 3.179319601386206 24.868712936262618 >50 0.680988703259115 14.804474294915535 >100 0.09309922148177587 5.38885226871209 >500 0.005216766720961579 1.130604655748357 >1k 0.003611607729896477 2.3021741997319345 >5k 4.0128974776627526E-4 0.6681740904776899 >10k+ 4.0128974776627526E-4 3.3226200694887837 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25306 3.258701438510779 No Hit GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC 5089 0.6553201462333579 No Hit CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC 4812 0.6196503328109487 No Hit CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT 2886 0.3716356734190353 Illumina Single End Adapter 2 (95% over 21bp) TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC 2036 0.2621795672491878 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC 1403 0.18066696112505426 No Hit CGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG 1397 0.17989432978738473 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG 1336 0.17203924452107802 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC 1290 0.1661157375989451 No Hit ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC 1234 0.15890451178069634 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC 1140 0.1467999541572073 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTTTTTTTTTT 850 0.10945610616984755 No Hit GCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG 793 0.1021161084619872 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2877188961158534E-4 0.0 0.0 0.39146654441921946 0.0 2 1.2877188961158534E-4 0.0 0.0 2.1927277363060753 0.0 3 1.2877188961158534E-4 0.0 0.0 3.0266544934307023 0.0 4 1.2877188961158534E-4 0.0 0.0 3.885305453360753 0.0 5 1.2877188961158534E-4 0.0 0.0 6.114475634426907 0.0 6 1.2877188961158534E-4 0.0 0.0 7.124948652209017 0.0 7 1.2877188961158534E-4 0.0 0.0 8.340555290142383 0.0 8 1.2877188961158534E-4 0.0 0.0 10.06481089204151 0.0 9 1.2877188961158534E-4 0.0 0.0 10.668622282430235 0.0 10 1.2877188961158534E-4 0.0 0.0 12.192251280314512 0.0 11 2.575437792231707E-4 0.0 0.0 14.257108530236284 0.0 12 2.575437792231707E-4 0.0 0.0 16.050514636856832 0.0 13 2.575437792231707E-4 0.0 0.0 16.666301812979434 0.0 14 2.575437792231707E-4 0.0 0.0 16.905044896319314 0.0 15 2.575437792231707E-4 0.0 0.0 17.361670016881995 0.0 16 2.575437792231707E-4 0.0 0.0 18.29294832255298 0.0 17 2.575437792231707E-4 0.0 0.0 19.47391532218083 0.0 18 2.575437792231707E-4 0.0 0.0 20.906760137888938 0.0 19 2.575437792231707E-4 0.0 0.0 21.728196021721242 0.0 20 2.575437792231707E-4 0.0 0.0 22.456916145033205 0.0 21 2.575437792231707E-4 0.0 0.0 23.29818289986569 0.0 22 2.575437792231707E-4 0.0 0.0 24.100560544035478 0.0 23 2.575437792231707E-4 0.0 0.0 24.87151784714004 0.0 24 2.575437792231707E-4 0.0 0.0 25.53198886895786 0.0 25 3.8631566883475606E-4 0.0 0.0 26.091760273099425 0.0 26 3.8631566883475606E-4 0.0 0.0 26.62448958042255 0.0 27 3.8631566883475606E-4 0.0 0.0 27.103392237888038 0.0 28 5.150875584463414E-4 0.0 0.0 27.592210330853614 0.0 29 5.150875584463414E-4 0.0 0.0 28.09622350679336 0.0 30 5.150875584463414E-4 0.0 0.0 28.650586491571236 0.0 31 5.150875584463414E-4 0.0 0.0 29.17893755464757 0.0 32 5.150875584463414E-4 0.0 0.0 29.648182320392188 0.0 33 5.150875584463414E-4 0.0 0.0 30.152324268221545 0.0 34 5.150875584463414E-4 0.0 0.0 30.651315340466436 0.0 35 5.150875584463414E-4 0.0 0.0 31.15352570995162 0.0 36 5.150875584463414E-4 0.0 0.0 31.640669768352247 0.0 37 5.150875584463414E-4 0.0 0.0 32.138501893590636 0.0 38 6.438594480579268E-4 0.0 0.0 32.72299750053762 0.0 39 6.438594480579268E-4 0.0 0.0 33.29538854986112 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGT 65 0.0 45.000004 4 GTTCGCG 70 0.0 45.000004 1 CGAATTT 35 1.2112469E-7 45.000004 1 CGAATCT 35 1.2112469E-7 45.000004 27 CTCGAGG 35 1.2112469E-7 45.000004 2 ACGCGAA 20 7.0318725E-4 45.0 11 CTTCGTC 20 7.0318725E-4 45.0 24 CTTCGGA 20 7.0318725E-4 45.0 13 CGGAATT 20 7.0318725E-4 45.0 14 AGTCCGA 20 7.0318725E-4 45.0 11 TCCGCTT 20 7.0318725E-4 45.0 20 CGTATTT 25 3.8896324E-5 45.0 1 CTATCAG 25 3.8896324E-5 45.0 1 GACACGT 20 7.0318725E-4 45.0 23 AGCCCGT 20 7.0318725E-4 45.0 34 GTTTACG 20 7.0318725E-4 45.0 1 TAGCGTG 20 7.0318725E-4 45.0 1 CGCATAC 25 3.8896324E-5 45.0 30 TTGTCGC 25 3.8896324E-5 45.0 43 ACCGAGT 20 7.0318725E-4 45.0 11 >>END_MODULE