##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1126077
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57749780876441	34.0	31.0	34.0	31.0	34.0
2	32.734173595588935	34.0	31.0	34.0	31.0	34.0
3	32.788869677650816	34.0	31.0	34.0	31.0	34.0
4	36.192943288958034	37.0	37.0	37.0	35.0	37.0
5	36.08281138856402	37.0	35.0	37.0	35.0	37.0
6	36.06883898703197	37.0	35.0	37.0	35.0	37.0
7	36.21473309551656	37.0	37.0	37.0	35.0	37.0
8	36.210347072180674	37.0	37.0	37.0	35.0	37.0
9	37.97882826840438	39.0	39.0	39.0	35.0	39.0
10	37.596849060943434	39.0	38.0	39.0	35.0	39.0
11	37.46437232977851	39.0	37.0	39.0	35.0	39.0
12	37.43260896013328	39.0	37.0	39.0	35.0	39.0
13	37.40415087067758	39.0	37.0	39.0	35.0	39.0
14	38.72204387444198	40.0	38.0	41.0	35.0	41.0
15	38.76035919390947	40.0	38.0	41.0	35.0	41.0
16	38.839156647369585	40.0	38.0	41.0	35.0	41.0
17	38.83007112302267	40.0	38.0	41.0	35.0	41.0
18	38.34461852963874	39.0	38.0	41.0	35.0	41.0
19	37.866016267093634	38.0	37.0	41.0	35.0	41.0
20	37.29106979362868	38.0	35.0	41.0	34.0	41.0
21	37.23957153906882	38.0	35.0	41.0	34.0	41.0
22	37.06254812059921	38.0	35.0	40.0	34.0	41.0
23	36.944035798617676	38.0	35.0	40.0	33.0	41.0
24	36.7353777761201	38.0	35.0	40.0	33.0	41.0
25	36.5959068518405	37.0	35.0	40.0	33.0	41.0
26	36.74212864662008	38.0	35.0	40.0	33.0	41.0
27	36.78055585896879	38.0	35.0	40.0	33.0	41.0
28	36.71125597983087	38.0	35.0	40.0	33.0	41.0
29	36.57389237148082	38.0	35.0	40.0	33.0	41.0
30	36.29503488660189	38.0	35.0	40.0	32.0	41.0
31	35.78803136908044	38.0	35.0	40.0	30.0	41.0
32	35.047907913934836	38.0	35.0	40.0	23.0	41.0
33	34.17206016995286	38.0	34.0	40.0	18.0	41.0
34	33.400325199786515	38.0	33.0	40.0	12.0	41.0
35	32.90604638936769	38.0	33.0	40.0	10.0	41.0
36	32.688007125622846	38.0	33.0	41.0	9.0	41.0
37	32.43852329814036	38.0	31.0	40.0	8.0	41.0
38	32.27747392052231	37.0	31.0	40.0	8.0	41.0
39	32.21056375363319	37.0	31.0	40.0	8.0	41.0
40	32.08938021112233	37.0	30.0	40.0	8.0	41.0
41	31.969140653791882	37.0	30.0	40.0	7.0	41.0
42	31.862211021093586	37.0	30.0	40.0	7.0	41.0
43	31.78693730535301	37.0	29.0	40.0	7.0	41.0
44	31.674339321378557	37.0	29.0	40.0	7.0	41.0
45	31.631528749810183	37.0	29.0	40.0	7.0	41.0
46	31.586757388704324	36.0	28.0	40.0	7.0	41.0
47	31.594485101818083	36.0	29.0	40.0	7.0	41.0
48	31.553568716881706	36.0	28.0	40.0	8.0	41.0
49	31.452799408921415	36.0	27.0	40.0	7.0	41.0
50	31.36711699111162	36.0	27.0	40.0	7.0	41.0
51	30.833244085439983	35.0	26.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	11.0
12	12.0
13	29.0
14	32.0
15	50.0
16	95.0
17	213.0
18	403.0
19	753.0
20	1392.0
21	2217.0
22	3554.0
23	5836.0
24	11012.0
25	23321.0
26	42535.0
27	55223.0
28	51794.0
29	41645.0
30	32965.0
31	28876.0
32	28089.0
33	33582.0
34	56895.0
35	75254.0
36	77992.0
37	102869.0
38	190268.0
39	258975.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.78792746854789	15.628948997271058	18.77553666401143	35.80758687016962
2	49.47068450914103	19.06175154984961	18.634338504382917	12.833225436626448
3	22.842931700052482	19.005805109242086	45.55283519688263	12.598427993822803
4	21.828436243702694	19.00669314798189	45.393165831466234	13.771704776849186
5	19.982914134646208	21.618237473991567	43.488233930716994	14.91061446064523
6	20.182012420109814	25.560863067090438	42.57035708925766	11.686767423542085
7	59.14977394973878	4.788748904382205	32.36617034181499	3.6953068040640207
8	59.72646630736619	4.977013117220226	31.266423166444213	4.030097408969368
9	54.854508173064545	6.33011774505651	33.003604549244855	5.8117695326340915
10	29.92965845142028	22.05364286811648	37.815087245365994	10.201611435097245
11	22.825170925256444	18.76541302237769	44.12691139238258	14.282504659983285
12	20.81589447258047	16.9015085114073	47.289483756439395	14.993113259572835
13	18.244134282113922	19.020013729078915	48.084278428562165	14.651573560244993
14	14.910703264519212	21.635909444913626	46.915441839234795	16.53794545133237
15	14.009077531998257	19.525041360404305	51.16550644405311	15.300374663544321
16	15.821031776690225	19.55416903106981	48.4743050430832	16.150494149156764
17	16.40900222631312	18.954831685577453	46.35819753000905	18.27796855810038
18	16.51245873950005	19.79527154892605	47.347472686148464	16.344797025425436
19	17.95614331879614	20.137521679245733	45.45639418974014	16.449940812217992
20	19.028006077737135	19.66979167499203	46.304648793998986	14.997553453271845
21	17.89042845205079	20.56005051164352	46.58367056604477	14.965850470260914
22	17.372257847376336	19.041859482078046	45.54821739543566	18.037665275109962
23	16.58394585805411	20.217977989071795	46.34567618377784	16.85239996909625
24	16.786241082981004	18.378760955067904	47.680309605826245	17.154688356124847
25	15.686849123106148	20.81145427888146	45.82706155973349	17.67463503827891
26	15.31804663446638	20.88231977031766	45.941352145546	17.85828144966996
27	16.36087052661585	20.72176236616146	45.407196843555106	17.510170263667582
28	14.724570344656716	20.49309239066245	47.07928498672826	17.703052277952576
29	16.089130672236447	18.830328654257215	46.650451079277886	18.43008959422846
30	16.962871988327617	19.926701282416744	44.70546863136357	18.404958097892063
31	18.006672723090873	20.986930733866334	42.84946766517743	18.15692887786537
32	17.700476965607148	22.052310810006777	42.81270286134962	17.434509363036454
33	18.63078634942371	22.222015013182936	40.917095367368304	18.23010327002505
34	17.233013372975385	23.052775254267694	40.4740528400811	19.24015853267583
35	16.953547581559697	24.029795475797837	38.016938450922986	20.99971849171948
36	18.989109980933808	25.00788134381574	36.71649452035696	19.286514154893496
37	18.240582127154713	25.6327942050144	38.161067138392845	17.96555652943804
38	18.412328819432418	26.632637022157457	35.693118676609146	19.26191548180098
39	19.43783595615575	25.18557789565012	37.0136322826947	18.362953865499428
40	19.313688140331433	25.42552596314462	36.53178246247814	18.729003434045808
41	16.952659542819895	26.506357913357615	35.00968406245754	21.531298481364953
42	17.877640694197645	25.78900021934557	35.34092251240368	20.992436574053105
43	19.572551432983712	24.699021470112612	35.151148633708	20.57727846319568
44	18.22433101821634	25.156805440480532	35.25673643987045	21.362127101432673
45	18.356915202068773	24.195414700770908	35.092449273007084	22.355220824153232
46	18.916912431387907	24.82831991062778	36.14814972688369	20.106617931100626
47	17.199267900862907	24.322137828940647	38.81173312304576	19.666861147150684
48	17.767967909832098	23.09202656656694	38.036208891576685	21.103796632024274
49	17.619132617041284	22.253185172949983	39.45387393579657	20.673808274212156
50	17.394636423619346	22.498816688379215	38.85418137480829	21.252365513193148
51	17.086131765412134	22.26908106639244	37.34469312489288	23.300094043302543
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	393.0
1	771.0
2	1149.0
3	45044.5
4	88940.0
5	61552.5
6	34165.0
7	32202.0
8	30239.0
9	28855.0
10	27471.0
11	26226.0
12	24981.0
13	23382.5
14	21784.0
15	19648.5
16	17513.0
17	15742.5
18	13972.0
19	13033.0
20	12094.0
21	11248.5
22	10403.0
23	9830.5
24	9258.0
25	9859.0
26	10640.0
27	10820.0
28	12281.5
29	13743.0
30	15365.5
31	16988.0
32	18199.5
33	19411.0
34	21370.5
35	23330.0
36	24327.0
37	25324.0
38	28144.5
39	30965.0
40	35061.0
41	39157.0
42	44693.0
43	50229.0
44	55293.5
45	60358.0
46	69595.5
47	78833.0
48	81423.0
49	84013.0
50	83403.5
51	82794.0
52	71318.0
53	59842.0
54	53668.0
55	47494.0
56	43051.0
57	38608.0
58	36283.0
59	33958.0
60	32811.0
61	31664.0
62	29255.0
63	26846.0
64	22324.0
65	17802.0
66	14893.0
67	11984.0
68	9974.0
69	7964.0
70	6416.5
71	4869.0
72	3780.0
73	2691.0
74	2079.0
75	1252.0
76	1037.0
77	736.5
78	436.0
79	358.0
80	280.0
81	270.0
82	260.0
83	150.5
84	41.0
85	30.0
86	19.0
87	16.0
88	13.0
89	9.5
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1126077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.72898829376341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8910617372792	25.49295647788214
2	9.712952291033613	6.3579020366224315
3	3.53751304269587	3.4733766889028557
4	1.7413475238632001	2.2797017089557023
5	0.9521019263897116	1.5580666401639232
6	0.6345714417366926	1.2461328772894065
7	0.44171573509861783	1.0119836386249632
8	0.34563944234827926	0.9049943409983795
9	0.2708986511322981	0.7979614903534752
>10	3.6567197654778236	30.674401576358274
>50	0.7276027520942657	15.000814453383512
>100	0.0809672245887442	4.758107609642998
>500	0.004421418407449701	1.0179342155456708
>1k	0.0022107092037248503	1.4979143916363749
>5k	0.0	0.0
>10k+	2.763386504656063E-4	3.9277518536399283
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43428	3.8565746392120612	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3379	0.30006829017909076	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3017	0.2679212877982589	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	2313	0.20540336051619915	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	2133	0.18941866319976344	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	1817	0.1613566390220207	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	1700	0.15096658576633748	No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1162	0.10319010156499067	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7760774796039702E-4	0.0	0.0	0.1609126196521197	0.0
2	1.7760774796039702E-4	0.0	0.0	0.9625451900713716	0.0
3	1.7760774796039702E-4	0.0	0.0	1.3326797368208392	0.0
4	1.7760774796039702E-4	0.0	0.0	1.7490811019139898	0.0
5	1.7760774796039702E-4	0.0	0.0	2.9053963450101548	0.0
6	1.7760774796039702E-4	0.0	0.0	3.439285235379108	0.0
7	1.7760774796039702E-4	0.0	0.0	4.016776827872339	0.0
8	1.7760774796039702E-4	0.0	0.0	4.863521766273532	0.0
9	1.7760774796039702E-4	0.0	0.0	5.165632545554168	0.0
10	1.7760774796039702E-4	0.0	0.0	6.027651750279954	0.0
11	1.7760774796039702E-4	0.0	0.0	7.0120426933504545	0.0
12	1.7760774796039702E-4	0.0	0.0	8.009043786526144	0.0
13	1.7760774796039702E-4	0.0	0.0	8.332112280066106	0.0
14	1.7760774796039702E-4	0.0	0.0	8.478993887629354	0.0
15	1.7760774796039702E-4	0.0	0.0	8.767162458695099	0.0
16	1.7760774796039702E-4	0.0	0.0	9.319344947103973	0.0
17	1.7760774796039702E-4	0.0	0.0	9.9851963942075	0.0
18	1.7760774796039702E-4	0.0	0.0	10.735145109970277	0.0
19	1.7760774796039702E-4	0.0	0.0	11.232979627503271	0.0
20	1.7760774796039702E-4	0.0	0.0	11.666164924778679	0.0
21	2.664116219405955E-4	0.0	0.0	12.200409030643552	0.0
22	3.5521549592079405E-4	0.0	0.0	12.75418998878407	0.0
23	3.5521549592079405E-4	0.0	0.0	13.29092060312039	0.0
24	3.5521549592079405E-4	0.0	0.0	13.730766190944314	0.0
25	3.5521549592079405E-4	0.0	0.0	14.120881609339326	0.0
26	3.5521549592079405E-4	0.0	0.0	14.497143623393427	0.0
27	3.5521549592079405E-4	0.0	0.0	14.858752998240796	0.0
28	3.5521549592079405E-4	0.0	0.0	15.220273569214184	0.0
29	3.5521549592079405E-4	0.0	0.0	15.604794343548443	0.0
30	3.5521549592079405E-4	0.0	0.0	16.038246052445793	0.0
31	3.5521549592079405E-4	0.0	0.0	16.45740033763233	0.0
32	3.5521549592079405E-4	0.0	0.0	16.83836895700738	0.0
33	3.5521549592079405E-4	8.880387398019851E-5	0.0	17.203530486813957	0.0
34	3.5521549592079405E-4	8.880387398019851E-5	0.0	17.57224417157974	0.0
35	3.5521549592079405E-4	8.880387398019851E-5	0.0	17.97816667954323	0.0
36	3.5521549592079405E-4	8.880387398019851E-5	0.0	18.369880567669885	0.0
37	3.5521549592079405E-4	8.880387398019851E-5	0.0	18.750049952179115	0.0
38	3.5521549592079405E-4	1.7760774796039702E-4	0.0	19.11041607279076	0.0
39	3.5521549592079405E-4	1.7760774796039702E-4	0.0	19.475133583227436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	45	3.8562575E-10	45.000004	2
GTCATCG	20	7.0332276E-4	45.0	43
TCCGCGA	20	7.0332276E-4	45.0	15
CTCGTAG	40	6.8157533E-9	45.0	1
CGCGGTC	20	7.0332276E-4	45.0	31
CGACGGT	20	7.0332276E-4	45.0	28
GCGAACG	20	7.0332276E-4	45.0	1
CGGTTGA	20	7.0332276E-4	45.0	41
GGCGTAC	20	7.0332276E-4	45.0	8
ATTCGTG	20	7.0332276E-4	45.0	12
TCTAGCG	25	3.890755E-5	45.0	1
TACGCGC	20	7.0332276E-4	45.0	34
ACGGAAC	20	7.0332276E-4	45.0	20
CTCGAAC	20	7.0332276E-4	45.0	21
CCACGCG	20	7.0332276E-4	45.0	17
TGTCGTA	20	7.0332276E-4	45.0	18
GTCGGTA	20	7.0332276E-4	45.0	41
GTTTACG	30	2.1654632E-6	44.999996	1
ACGGTTG	30	2.1654632E-6	44.999996	1
CGTTTTT	29250	0.0	44.376923	1
>>END_MODULE