SRR3549885_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549885_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1245065
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47472	3.8128129856674153	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2607	0.20938665852786803	RNA PCR Primer, Index 30 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2453	0.19701782637854248	RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1462	0.11742358832671387	No Hit
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	1372	0.1101950500576275	RNA PCR Primer, Index 30 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTC	25	3.890993E-5	45.000004	12
CGCCTAT	20	7.033512E-4	45.000004	38
AGACGTC	20	7.033512E-4	45.000004	16
CTACCGT	25	3.890993E-5	45.000004	19
TTACGTG	20	7.033512E-4	45.000004	23
ATCGCGA	25	3.890993E-5	45.000004	12
GCGTACA	20	7.033512E-4	45.000004	1
TCGAACC	20	7.033512E-4	45.000004	14
ACGATAG	35	1.212029E-7	45.0	1
CGTTTTT	35305	0.0	44.458294	1
CGATAGA	60	3.6379788E-12	41.250004	10
GTTTTTT	39410	0.0	40.152878	2
TTTAGCG	40	3.4585355E-7	39.375004	1
CGATCGC	40	3.4585355E-7	39.375004	10
CCGTTCG	35	6.248709E-6	38.571426	5
CACAACG	65	9.094947E-12	38.076927	12
GGCTACG	65	9.094947E-12	38.076927	1
CGCATGG	125	0.0	37.8	2
CCTACCG	30	1.13997645E-4	37.500004	18
TTATCGG	30	1.13997645E-4	37.500004	2