FastQCFastQC Report
Sat 18 Jun 2016
SRR3549884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031305
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352023.413345227648465No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC25810.2502654403886338No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC24180.23446022272751513No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT13330.12925371252927115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC207.0329476E-445.00000437
CGAAACT207.0329476E-445.00000444
ACGCATG207.0329476E-445.00000426
AACGCAT207.0329476E-445.00000425
TTCGGTA207.0329476E-445.00000425
AACCTCG207.0329476E-445.00000412
TATAGCG207.0329476E-445.0000041
CAGTCGA207.0329476E-445.00000428
CGTCCTC207.0329476E-445.00000431
CGATCTA406.8139343E-945.00000410
TACGTTG406.8139343E-945.0000041
GTCGACG253.890527E-545.030
CCTCGCA253.890527E-545.037
ACGATTC253.890527E-545.017
TCGTCGG253.890527E-545.02
TTGCGAA253.890527E-545.023
ATAACGG253.890527E-545.02
TATCGTG253.890527E-545.01
CAACGTA302.165285E-644.99999635
ACGTTAG302.165285E-644.9999961