##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1031305
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.618556101250356	34.0	31.0	34.0	31.0	34.0
2	32.777698159128484	34.0	31.0	34.0	31.0	34.0
3	32.821289531225	34.0	31.0	34.0	31.0	34.0
4	36.203961000867835	37.0	37.0	37.0	35.0	37.0
5	36.10419807913275	37.0	35.0	37.0	35.0	37.0
6	36.102141461546296	37.0	36.0	37.0	35.0	37.0
7	36.25036046562365	37.0	37.0	37.0	35.0	37.0
8	36.2551146363103	37.0	37.0	37.0	35.0	37.0
9	38.02643349930428	39.0	39.0	39.0	35.0	39.0
10	37.64400347133002	39.0	38.0	39.0	35.0	39.0
11	37.55649686562171	39.0	37.0	39.0	35.0	39.0
12	37.44770460726943	39.0	37.0	39.0	35.0	39.0
13	37.37587813498432	39.0	37.0	39.0	35.0	39.0
14	38.65173930117666	40.0	38.0	41.0	35.0	41.0
15	38.73174085260907	40.0	38.0	41.0	35.0	41.0
16	38.820248132220826	40.0	38.0	41.0	35.0	41.0
17	38.82483164534255	40.0	38.0	41.0	35.0	41.0
18	38.33219658587905	39.0	38.0	41.0	35.0	41.0
19	37.82901275568333	38.0	37.0	41.0	35.0	41.0
20	37.26024018112973	38.0	35.0	41.0	34.0	41.0
21	37.20730239841754	38.0	35.0	41.0	34.0	41.0
22	37.03027717309622	38.0	35.0	40.0	34.0	41.0
23	36.913351530342624	38.0	35.0	40.0	34.0	41.0
24	36.70111848580197	37.0	35.0	40.0	33.0	41.0
25	36.563559761661196	37.0	35.0	40.0	33.0	41.0
26	36.66789746971071	37.0	35.0	40.0	33.0	41.0
27	36.69792641362157	37.0	35.0	40.0	33.0	41.0
28	36.612434730753755	37.0	35.0	40.0	33.0	41.0
29	36.489446865864124	37.0	35.0	40.0	33.0	41.0
30	36.14276087093537	37.0	35.0	40.0	32.0	41.0
31	35.58844861607381	37.0	35.0	40.0	30.0	41.0
32	34.822519041408704	37.0	35.0	40.0	22.0	41.0
33	33.79677011165465	37.0	34.0	40.0	15.0	41.0
34	32.975820925914256	37.0	33.0	40.0	10.0	41.0
35	32.490744251215695	37.0	32.0	40.0	10.0	41.0
36	32.270142198476684	37.0	31.0	40.0	8.0	41.0
37	32.04644988630909	37.0	31.0	40.0	8.0	41.0
38	31.90594537988277	37.0	30.0	40.0	8.0	41.0
39	31.847023916300223	37.0	30.0	40.0	8.0	41.0
40	31.696676540887516	36.0	29.0	40.0	7.0	41.0
41	31.544132918971595	36.0	29.0	40.0	7.0	41.0
42	31.4111586775978	36.0	28.0	40.0	7.0	41.0
43	31.26136109104484	36.0	26.0	40.0	7.0	41.0
44	31.123589044947906	36.0	25.0	40.0	7.0	41.0
45	31.062316191621296	35.0	24.0	40.0	7.0	41.0
46	30.983580027247033	35.0	24.0	40.0	7.0	41.0
47	30.932872428621987	35.0	24.0	40.0	7.0	41.0
48	30.865193129093722	35.0	24.0	40.0	7.0	41.0
49	30.733623903694834	35.0	24.0	40.0	7.0	41.0
50	30.637471940890425	35.0	24.0	40.0	7.0	41.0
51	30.123151734937775	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	10.0
11	10.0
12	19.0
13	11.0
14	37.0
15	39.0
16	103.0
17	183.0
18	397.0
19	716.0
20	1269.0
21	1990.0
22	3324.0
23	5657.0
24	10991.0
25	24064.0
26	43850.0
27	54514.0
28	49776.0
29	39005.0
30	29873.0
31	26482.0
32	25430.0
33	30351.0
34	55721.0
35	75129.0
36	69215.0
37	89790.0
38	165334.0
39	227858.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.119023955086032	15.282578868520952	17.79163293109216	36.806764245300855
2	51.06549468876811	17.463892834806387	17.493660944143585	13.976951532281914
3	23.64382990482932	18.202665554806774	45.4471761506053	12.706328389758607
4	21.64102763004155	18.976248539471836	45.56440626196906	13.818317568517557
5	18.998453415817824	22.057587231711278	44.41760681854544	14.526352533925463
6	20.156985566830375	24.41043144365634	43.8083787046509	11.624204284862383
7	59.03510600646753	4.660018132366274	32.569511444238124	3.7353644169280673
8	59.35954930888534	4.2151448892422705	32.4872855265901	3.9380202752822875
9	54.811525203504296	6.1055652789426995	34.10155094758582	4.981358569967178
10	30.49437363340622	19.723069315091074	39.12062871798352	10.661928333519182
11	25.336636591502998	19.218562888767142	42.621920770286195	12.822879749443667
12	23.811772463044395	16.474757709891836	46.07250037573753	13.640969451326232
13	18.692627302301453	19.307382394151098	46.35728518721426	15.64270511633319
14	13.402145824949944	22.78559688937802	46.63150086540839	17.180756420263645
15	13.940783764259844	19.492778566961277	52.23605044094618	14.330387227832697
16	15.867662815558928	18.096683328404303	50.77896451583188	15.256689340204884
17	14.732402150673177	17.752459262778714	47.869640891879705	19.645497694668407
18	16.782426149393245	19.20896340073984	47.40236884335866	16.60624160650826
19	18.923693766635477	20.41006297845933	45.19516534875716	15.471077906148038
20	21.489762970217345	19.091927218427138	45.18925051269993	14.229059298655585
21	18.905270506785094	19.991176228176922	45.617930680060695	15.485622584977285
22	17.048011984815357	19.7943382413544	44.96031726792753	18.197332505902715
23	16.84913774295674	20.759426163937924	45.684060486471026	16.70737560663431
24	17.790275427734763	17.75604695022326	47.418174061019776	17.0355035610222
25	16.955701756512383	19.582761646651573	45.33944856274332	18.122088034092727
26	16.1902637919917	21.358860860754092	45.82126529009362	16.629610057160587
27	16.534390893091764	21.68592220536117	45.80138756236031	15.978299339186758
28	14.488924227071525	21.9666345067657	47.76462831073252	15.779812955430256
29	17.092518702032862	19.539515468265936	46.66592327197095	16.702042557730255
30	16.73423478020566	20.573933026602216	45.98629891254284	16.705533280649274
31	16.73268334779721	22.031309845293098	43.54647752119887	17.689529285710822
32	19.213908591541784	22.32297913808233	41.809067152782156	16.65404511759373
33	18.976927291150535	24.609984437193653	39.04887496909256	17.364213302563257
34	17.900330164209425	24.241325311134922	38.80956652008862	19.04877800456703
35	17.975477671493884	25.22299416758379	35.96394858940857	20.83757957151376
36	18.732285793242543	28.509800689417773	34.38313592972011	18.37477758761957
37	19.039857268218423	27.34932924789466	35.9548339240089	17.65597955987802
38	18.738976345503996	27.008789834239145	34.49086351758209	19.761370302674766
39	20.181905449891158	25.415565715283062	34.87532786130194	19.527200973523836
40	20.2463868593675	24.83057873277062	35.60644038378559	19.31659402407629
41	17.662088324986303	26.545008508637114	34.24331308390825	21.549590082468328
42	19.746922588371042	26.289506983869952	33.25437188804476	20.709198539714247
43	20.002715006714794	27.12621387465396	32.9270196498611	19.944051468770148
44	19.017652391872435	27.28921124206709	32.82510993353082	20.86802643252966
45	18.853297521101904	26.069397510920627	32.49766073082163	22.579644237155836
46	18.30719331332632	27.32576686819127	32.91751712635932	21.44952269212309
47	17.668487983671174	25.787133777107645	35.54496487460062	20.999413364620555
48	17.97751392652998	23.80159118786392	35.784370288130084	22.436524597476012
49	18.12005177905663	23.686494296061785	36.56891026417985	21.624543660701733
50	16.296924770072867	24.344204672720483	36.13450918981291	23.22436136739374
51	16.26832023504201	24.20506057858732	34.416103868399745	25.110515317970922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	663.5
2	1027.0
3	39539.0
4	78051.0
5	53865.5
6	29680.0
7	28753.0
8	27826.0
9	27317.5
10	26809.0
11	25500.0
12	24191.0
13	22435.5
14	20680.0
15	19319.0
16	17958.0
17	16689.5
18	15421.0
19	14436.0
20	13451.0
21	12111.0
22	10771.0
23	10059.5
24	9348.0
25	9431.0
26	9777.0
27	10040.0
28	10752.0
29	11464.0
30	12095.5
31	12727.0
32	14133.5
33	15540.0
34	18180.5
35	20821.0
36	22103.0
37	23385.0
38	25002.5
39	26620.0
40	31135.0
41	35650.0
42	40335.5
43	45021.0
44	48065.5
45	51110.0
46	56096.5
47	61083.0
48	65464.5
49	69846.0
50	69735.5
51	69625.0
52	62381.5
53	55138.0
54	50208.0
55	45278.0
56	41147.5
57	37017.0
58	35625.0
59	34233.0
60	35914.5
61	37596.0
62	35201.5
63	32807.0
64	27462.5
65	22118.0
66	16477.0
67	10836.0
68	8805.5
69	6775.0
70	5285.5
71	3796.0
72	3212.5
73	2629.0
74	2270.0
75	1897.5
76	1884.0
77	1313.5
78	743.0
79	546.0
80	349.0
81	254.0
82	159.0
83	108.0
84	57.0
85	30.0
86	3.0
87	7.5
88	12.0
89	7.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1031305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.38788508172846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13715344420427	26.08834299808475
2	9.54436547488976	6.37332355307272
3	3.5648540710462147	3.5706881417166865
4	1.7381548909164755	2.321332630086542
5	1.0286786531775678	1.717270232915992
6	0.6408537264923666	1.2838050344594747
7	0.46596753355939297	1.0890369323608224
8	0.33516690282563927	0.8952411227793035
9	0.2655220963530971	0.7978699115727154
>10	3.4112292162136773	29.16707541454848
>50	0.7709125941057047	16.342720353826277
>100	0.0921066287291332	5.289125673187373
>500	0.0038501163134287563	0.9571825331636757
>1k	8.884883800220207E-4	0.6261239462802061
>5k	0.0	0.0
>10k+	2.961627933406736E-4	3.480861521945012
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35202	3.413345227648465	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	2581	0.2502654403886338	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	2418	0.23446022272751513	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	1333	0.12925371252927115	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.908935765850064E-4	0.0	0.0	0.13691391004600967	0.0
2	2.908935765850064E-4	0.0	0.0	0.8204168504952463	0.0
3	2.908935765850064E-4	0.0	0.0	1.1847125729052026	0.0
4	3.8785810211334186E-4	0.0	0.0	1.5298093192605486	0.0
5	4.848226276416773E-4	0.0	0.0	2.4539782120711138	0.0
6	4.848226276416773E-4	0.0	0.0	2.950048724674078	0.0
7	4.848226276416773E-4	0.0	0.0	3.472396623695221	0.0
8	4.848226276416773E-4	0.0	0.0	4.277299150105934	0.0
9	4.848226276416773E-4	0.0	0.0	4.571586485084432	0.0
10	4.848226276416773E-4	0.0	0.0	5.246653511812704	0.0
11	4.848226276416773E-4	0.0	0.0	6.250818138184146	0.0
12	4.848226276416773E-4	0.0	0.0	7.012377521683692	0.0
13	5.817871531700128E-4	0.0	0.0	7.335366356218578	0.0
14	5.817871531700128E-4	0.0	0.0	7.465298820426547	0.0
15	5.817871531700128E-4	0.0	0.0	7.762689020221952	0.0
16	5.817871531700128E-4	0.0	0.0	8.304138930772178	0.0
17	5.817871531700128E-4	0.0	0.0	8.975909163632485	0.0
18	5.817871531700128E-4	0.0	0.0	9.756279665084529	0.0
19	5.817871531700128E-4	0.0	0.0	10.161688346318499	0.0
20	5.817871531700128E-4	0.0	0.0	10.571460431201245	0.0
21	6.787516786983482E-4	0.0	0.0	11.072476134606154	0.0
22	6.787516786983482E-4	0.0	0.0	11.599769224429243	0.0
23	6.787516786983482E-4	0.0	0.0	12.154891133078962	0.0
24	6.787516786983482E-4	0.0	0.0	12.57280823810609	0.0
25	6.787516786983482E-4	0.0	0.0	12.970750650874377	0.0
26	6.787516786983482E-4	0.0	0.0	13.316332219857365	0.0
27	6.787516786983482E-4	0.0	0.0	13.663174327672222	0.0
28	6.787516786983482E-4	0.0	0.0	14.021264320448363	0.0
29	6.787516786983482E-4	0.0	0.0	14.380808781107431	0.0
30	6.787516786983482E-4	0.0	0.0	14.797077489200577	0.0
31	6.787516786983482E-4	0.0	0.0	15.20287402853666	0.0
32	6.787516786983482E-4	0.0	0.0	15.61991845283403	0.0
33	6.787516786983482E-4	0.0	0.0	16.002928328670954	0.0
34	6.787516786983482E-4	0.0	0.0	16.376144787429517	0.0
35	6.787516786983482E-4	0.0	0.0	16.769432902972447	0.0
36	6.787516786983482E-4	0.0	0.0	17.18579857559112	0.0
37	6.787516786983482E-4	0.0	0.0	17.585680278869976	0.0
38	6.787516786983482E-4	0.0	0.0	17.989634492221022	0.0
39	6.787516786983482E-4	0.0	0.0	18.346948768792938	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0329476E-4	45.000004	37
CGAAACT	20	7.0329476E-4	45.000004	44
ACGCATG	20	7.0329476E-4	45.000004	26
AACGCAT	20	7.0329476E-4	45.000004	25
TTCGGTA	20	7.0329476E-4	45.000004	25
AACCTCG	20	7.0329476E-4	45.000004	12
TATAGCG	20	7.0329476E-4	45.000004	1
CAGTCGA	20	7.0329476E-4	45.000004	28
CGTCCTC	20	7.0329476E-4	45.000004	31
CGATCTA	40	6.8139343E-9	45.000004	10
TACGTTG	40	6.8139343E-9	45.000004	1
GTCGACG	25	3.890527E-5	45.0	30
CCTCGCA	25	3.890527E-5	45.0	37
ACGATTC	25	3.890527E-5	45.0	17
TCGTCGG	25	3.890527E-5	45.0	2
TTGCGAA	25	3.890527E-5	45.0	23
ATAACGG	25	3.890527E-5	45.0	2
TATCGTG	25	3.890527E-5	45.0	1
CAACGTA	30	2.165285E-6	44.999996	35
ACGTTAG	30	2.165285E-6	44.999996	1
>>END_MODULE