FastQCFastQC Report
Sat 18 Jun 2016
SRR3549880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727090
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT613243.55071247010868No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC40770.23606181496042475TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC35300.20439004336774577TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT21460.12425525016067489TruSeq Adapter, Index 22 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG207.034272E-445.00000436
CGTTTTT446850.044.3353461
AATAGCG603.6379788E-1241.2499961
GTTTTTT495900.040.108892
ACGGGTA1250.039.6000025
TCGTTAG403.4595723E-739.3750041
CGTAACG403.4595723E-739.3750042
GCGTAAG800.039.3750041
TAACGCC356.250115E-638.57142612
CGGTCTA356.250115E-638.57142631
CGGTAAG700.038.5714261
GTACGCG700.038.5714261
GCGAGAC1600.037.96875421
CGTAAGG1250.037.8000032
CACGTGA1550.037.74193643
CGTATCT301.1401597E-437.49999640
CTACGCG601.5643309E-1037.4999961
ACACGAC1650.036.8181826
TCGACGG552.750312E-936.818182
AACACGT1650.036.8181841
Produced by FastQC (version 0.11.5)