##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1727090
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57698614432369	34.0	31.0	34.0	31.0	34.0
2	32.73270124892159	34.0	31.0	34.0	31.0	34.0
3	32.78560179261069	34.0	31.0	34.0	31.0	34.0
4	36.19311211343937	37.0	37.0	37.0	35.0	37.0
5	36.07445008656179	37.0	35.0	37.0	35.0	37.0
6	36.06537760047247	37.0	35.0	37.0	35.0	37.0
7	36.21149158410969	37.0	37.0	37.0	35.0	37.0
8	36.218498746446336	37.0	37.0	37.0	35.0	37.0
9	37.971239483755916	39.0	39.0	39.0	35.0	39.0
10	37.54381763544459	39.0	37.0	39.0	35.0	39.0
11	37.444032447643146	39.0	37.0	39.0	35.0	39.0
12	37.39243177830918	39.0	37.0	39.0	35.0	39.0
13	37.381370976613844	39.0	37.0	39.0	34.0	39.0
14	38.7045156882386	40.0	38.0	41.0	35.0	41.0
15	38.7406660915181	40.0	38.0	41.0	35.0	41.0
16	38.83633626504699	40.0	38.0	41.0	35.0	41.0
17	38.83220909159338	40.0	38.0	41.0	35.0	41.0
18	38.36241249732208	39.0	38.0	41.0	35.0	41.0
19	37.90747442229415	38.0	37.0	41.0	35.0	41.0
20	37.368005141596555	38.0	35.0	41.0	34.0	41.0
21	37.28762137468227	38.0	35.0	41.0	34.0	41.0
22	37.09894967836071	38.0	35.0	41.0	34.0	41.0
23	36.988872033304574	38.0	35.0	40.0	33.0	41.0
24	36.770068728323366	38.0	35.0	40.0	33.0	41.0
25	36.613413892732865	38.0	35.0	40.0	33.0	41.0
26	36.728601867881814	38.0	35.0	40.0	33.0	41.0
27	36.7685436196145	38.0	35.0	40.0	33.0	41.0
28	36.70142899327771	38.0	35.0	40.0	33.0	41.0
29	36.57485655061404	38.0	35.0	40.0	33.0	41.0
30	36.28670712006902	38.0	35.0	40.0	32.0	41.0
31	35.780848131828684	38.0	35.0	40.0	30.0	41.0
32	35.107451261949294	38.0	35.0	40.0	23.0	41.0
33	34.20734414535433	38.0	34.0	40.0	18.0	41.0
34	33.437916379574894	38.0	33.0	40.0	12.0	41.0
35	32.955037085502205	38.0	33.0	41.0	10.0	41.0
36	32.71817623864419	38.0	33.0	41.0	10.0	41.0
37	32.470705637806944	38.0	31.0	40.0	8.0	41.0
38	32.33024741038394	37.0	31.0	40.0	8.0	41.0
39	32.24363987979781	37.0	31.0	40.0	8.0	41.0
40	32.105859567248956	37.0	30.0	40.0	8.0	41.0
41	31.96480669797173	37.0	30.0	40.0	8.0	41.0
42	31.854062613992323	37.0	30.0	40.0	7.0	41.0
43	31.754668257010348	37.0	29.0	40.0	7.0	41.0
44	31.634700565691425	37.0	29.0	40.0	7.0	41.0
45	31.579302178809442	36.0	28.0	40.0	7.0	41.0
46	31.520246773474458	36.0	28.0	40.0	7.0	41.0
47	31.494794133484646	36.0	28.0	40.0	7.0	41.0
48	31.442824056650203	36.0	28.0	40.0	7.0	41.0
49	31.310239188461516	36.0	27.0	40.0	7.0	41.0
50	31.21560196631328	35.0	27.0	40.0	7.0	41.0
51	30.657976712273246	35.0	26.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	13.0
11	22.0
12	24.0
13	32.0
14	40.0
15	95.0
16	169.0
17	362.0
18	667.0
19	1184.0
20	1995.0
21	3253.0
22	5328.0
23	8648.0
24	17007.0
25	35787.0
26	65583.0
27	86025.0
28	81260.0
29	63612.0
30	49130.0
31	42608.0
32	41897.0
33	51335.0
34	88682.0
35	113778.0
36	122133.0
37	163522.0
38	294295.0
39	388362.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.219791672698005	15.88481202485105	17.91927461799906	35.97612168445188
2	50.54421020328993	17.77255383332658	18.489308605805142	13.193927357578355
3	23.70160211685552	18.562089989519944	44.4294738548657	13.306834038758836
4	22.200522265776538	19.674770857338068	44.078999936309046	14.045706940576345
5	19.826760620465638	22.30949168833124	43.15797092218703	14.70577676901609
6	20.68971506985739	25.104018898841403	42.46206046008025	11.744205571220956
7	60.53801481104054	4.867435976121684	30.925371578782805	3.6691776340549707
8	61.20810148863116	4.133889953621409	30.706853725051964	3.951154832695459
9	56.25427742619088	6.206914520957217	32.464550197152434	5.0742578556994715
10	30.730650979393083	20.350010711659497	37.89958832487016	11.019749984077261
11	24.21599337614137	19.34149349483814	42.244584821867996	14.197928307152493
12	21.945005761135782	17.21595284553787	45.434748623407	15.404292769919344
13	18.654962972398657	18.79606737344319	46.61934236200777	15.92962729215038
14	15.618989166748692	21.28273569993457	45.92430041283315	17.173974720483585
15	14.609545536133034	19.778934508334828	49.79769438766943	15.813825567862704
16	17.019668922870263	18.830344683832344	48.11069486824659	16.039291525050807
17	16.585817762826487	19.03687705909941	45.84387611531536	18.533429062758742
18	17.22469587572159	19.340567081043837	46.41472071519145	17.020016328043123
19	18.638229623239088	19.69162000822192	44.832116450213945	16.838033918325042
20	20.82086052261318	19.154010503216394	44.76251961391705	15.262609360253373
21	18.879618317516748	19.813732926483276	45.40226623974431	15.904382516255666
22	17.92981257490924	19.105663283326287	44.480426613552275	18.484097528212196
23	17.670069307331985	19.932892900775293	45.12891626956325	17.26812152232947
24	17.72837547551083	18.50083087737176	45.881916981743856	17.888876665373548
25	17.158109884256177	19.749173465192897	44.78295861825383	18.3097580322971
26	16.86675274594839	21.165544354955447	44.83657481659902	17.13112808249715
27	17.437365742375903	20.56493871193742	44.580421402474684	17.41727414321199
28	15.564736058919918	20.697763289695384	45.723558123780464	18.013942527604236
29	17.727680665165106	19.25991118007747	45.02620014012009	17.986208014637338
30	17.802662281641375	19.819696715284092	43.85961356964605	18.518027433428482
31	18.011510691394193	20.750742578556995	42.56888755073563	18.66885917931318
32	19.391346137143984	20.89659485029732	42.03255186469727	17.679507147861433
33	18.63249743788685	22.512492111007532	39.87921880156796	18.975791649537662
34	18.661505769820913	22.98670017196556	39.087192908302406	19.264601149911122
35	18.771575308756347	23.35651297847825	37.35317788881877	20.51873382394664
36	19.74894186174432	25.638675459877597	35.41616244665883	19.19622023171925
37	19.11486952040716	25.633348580560366	36.427632607449524	18.824149291582952
38	18.992003890937937	25.763741322108284	34.97281554522347	20.27143924173031
39	20.307279875397345	24.677173743117034	35.83177483512729	19.183771546358326
40	20.635172457717896	24.31361422971588	36.70937820264143	18.341835109924787
41	18.153309902784454	25.698313347885748	35.47290529155979	20.675471457770005
42	19.17763405497108	25.611056748634986	34.821173187268755	20.390136009125175
43	19.843030762728056	24.83339026918111	34.45141828161821	20.872160686472622
44	19.432861055301114	25.461383019993168	33.80385503940154	21.301900885304182
45	19.39979966301698	24.395659751373696	34.13215292775709	22.072387657852225
46	19.377913137126612	25.179811127387687	34.92365771326335	20.518618022222352
47	18.300841299526947	24.145470126050178	37.329843841374796	20.223844733048075
48	18.26494276499777	23.043500917728664	37.14143443595875	21.550121881314812
49	18.52480183429931	22.8979960511612	37.994487837923906	20.58271427661558
50	17.720732561707845	22.495353455812957	37.65484138058815	22.129072601891043
51	17.868669264485348	22.300285451250367	36.16464689159222	23.66639839267207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	357.0
1	853.5
2	1350.0
3	63419.5
4	125489.0
5	88555.0
6	51621.0
7	49017.0
8	46413.0
9	44745.5
10	43078.0
11	40802.0
12	38526.0
13	35714.5
14	32903.0
15	30079.0
16	27255.0
17	24898.0
18	22541.0
19	20999.0
20	19457.0
21	17862.5
22	16268.0
23	15340.0
24	14412.0
25	14250.5
26	15314.0
27	16539.0
28	17551.0
29	18563.0
30	20619.0
31	22675.0
32	25792.5
33	28910.0
34	30877.0
35	32844.0
36	35130.0
37	37416.0
38	40971.0
39	44526.0
40	49988.5
41	55451.0
42	60833.0
43	66215.0
44	74643.5
45	83072.0
46	93586.5
47	104101.0
48	111580.5
49	119060.0
50	120902.5
51	122745.0
52	108350.5
53	93956.0
54	84876.0
55	75796.0
56	71638.0
57	67480.0
58	66839.0
59	66198.0
60	64505.0
61	62812.0
62	56936.5
63	51061.0
64	43066.0
65	35071.0
66	28998.5
67	22926.0
68	19168.5
69	15411.0
70	13155.5
71	10900.0
72	9471.0
73	8042.0
74	6577.0
75	4462.0
76	3812.0
77	2666.0
78	1520.0
79	1112.0
80	704.0
81	467.0
82	230.0
83	158.5
84	87.0
85	60.0
86	33.0
87	35.0
88	37.0
89	24.0
90	11.0
91	10.5
92	10.0
93	7.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1727090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.390589569319854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4632795868371	24.316180162038766
2	9.601871827363555	6.028168352599691
3	3.6078506975872235	3.3975768142603453
4	1.8535901583647603	2.3274115156383512
5	1.0068357682919447	1.5802584183081632
6	0.6889700686136949	1.297630598963918
7	0.47399786208881195	1.041535064189548
8	0.36774544479918425	0.9234997058942531
9	0.2892488899367699	0.8171723868647864
>10	3.6363434699549764	29.10168963868581
>50	0.9163683773465946	18.392598240137715
>100	0.08729860465464348	5.045388723358006
>500	0.004525195995990977	0.9792473310291452
>1k	0.0018854983316629072	1.1220653310154447
>5k	0.0	0.0
>10k+	1.8854983316629072E-4	3.6295777170160957
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61324	3.55071247010868	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	4077	0.23606181496042475	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	3530	0.20439004336774577	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	2146	0.12425525016067489	TruSeq Adapter, Index 22 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7370258643151196E-4	0.0	0.0	0.13224556913652444	0.0
2	1.7370258643151196E-4	0.0	0.0	0.7432154664782958	0.0
3	1.7370258643151196E-4	0.0	0.0	1.0609753979236751	0.0
4	1.7370258643151196E-4	0.0	0.0	1.3882310707606436	0.0
5	1.7370258643151196E-4	0.0	0.0	2.3133131452327325	0.0
6	1.7370258643151196E-4	0.0	0.0	2.823477641582083	0.0
7	1.7370258643151196E-4	0.0	0.0	3.3587132112397153	0.0
8	1.7370258643151196E-4	0.0	0.0	4.215298565795645	0.0
9	1.7370258643151196E-4	0.0	0.0	4.531610975687428	0.0
10	1.7370258643151196E-4	0.0	0.0	5.242228256778743	0.0
11	1.7370258643151196E-4	0.0	0.0	6.226079706326828	0.0
12	1.7370258643151196E-4	0.0	0.0	7.028469853916125	0.0
13	1.7370258643151196E-4	0.0	0.0	7.343624246565032	0.0
14	1.7370258643151196E-4	0.0	0.0	7.464868651894227	0.0
15	1.7370258643151196E-4	0.0	0.0	7.729591393615851	0.0
16	1.7370258643151196E-4	0.0	0.0	8.249483234805366	0.0
17	1.7370258643151196E-4	0.0	0.0	8.900231024439954	0.0
18	2.316034485753493E-4	0.0	0.0	9.609458684839817	0.0
19	2.895043107191866E-4	0.0	0.0	10.009669443978021	0.0
20	2.895043107191866E-4	0.0	0.0	10.405827142766157	0.0
21	2.895043107191866E-4	0.0	0.0	10.900474208060958	0.0
22	2.895043107191866E-4	0.0	0.0	11.421234562182631	0.0
23	2.895043107191866E-4	0.0	0.0	11.937189144746366	0.0
24	3.4740517286302393E-4	0.0	0.0	12.33259413232663	0.0
25	3.4740517286302393E-4	0.0	0.0	12.695343033657771	0.0
26	3.4740517286302393E-4	0.0	0.0	13.027578180639109	0.0
27	3.4740517286302393E-4	0.0	0.0	13.364734900902674	0.0
28	4.0530603500686126E-4	0.0	0.0	13.701486315131232	0.0
29	4.0530603500686126E-4	0.0	0.0	14.055665888865086	0.0
30	4.0530603500686126E-4	0.0	0.0	14.440185514362309	0.0
31	4.0530603500686126E-4	0.0	0.0	14.816946424332258	0.0
32	4.0530603500686126E-4	0.0	0.0	15.178305704971947	0.0
33	4.632068971506986E-4	0.0	0.0	15.534975015777984	0.0
34	5.211077592945359E-4	0.0	0.0	15.870278908452947	0.0
35	5.211077592945359E-4	0.0	0.0	16.234591133061972	0.0
36	5.211077592945359E-4	0.0	0.0	16.611120439583345	0.0
37	5.211077592945359E-4	0.0	0.0	16.97589587108952	0.0
38	5.211077592945359E-4	0.0	0.0	17.342639931908586	0.0
39	5.790086214383732E-4	0.0	0.0	17.685123531489385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	20	7.034272E-4	45.000004	36
CGTTTTT	44685	0.0	44.335346	1
AATAGCG	60	3.6379788E-12	41.249996	1
GTTTTTT	49590	0.0	40.10889	2
ACGGGTA	125	0.0	39.600002	5
TCGTTAG	40	3.4595723E-7	39.375004	1
CGTAACG	40	3.4595723E-7	39.375004	2
GCGTAAG	80	0.0	39.375004	1
TAACGCC	35	6.250115E-6	38.571426	12
CGGTCTA	35	6.250115E-6	38.571426	31
CGGTAAG	70	0.0	38.571426	1
GTACGCG	70	0.0	38.571426	1
GCGAGAC	160	0.0	37.968754	21
CGTAAGG	125	0.0	37.800003	2
CACGTGA	155	0.0	37.741936	43
CGTATCT	30	1.1401597E-4	37.499996	40
CTACGCG	60	1.5643309E-10	37.499996	1
ACACGAC	165	0.0	36.81818	26
TCGACGG	55	2.750312E-9	36.81818	2
AACACGT	165	0.0	36.81818	41
>>END_MODULE