FastQCFastQC Report
Sat 18 Jun 2016
SRR3549878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275580
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470263.6866366672415682No Hit
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC19540.1531852177048872RNA PCR Primer, Index 17 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC18330.14369933677229182RNA PCR Primer, Index 47 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG14760.11571206823562616No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA13200.10348233744649493No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC12790.10026811332883864No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATC207.033579E-445.011
TATAGCG207.033579E-445.01
TCGGTAA253.891047E-545.033
CTATGCG302.1656906E-644.9999961
ACGTTAG302.1656906E-644.9999961
TATACGG302.1656906E-644.9999962
CGTTTTT358700.044.435461
CAGTACG556.184564E-1140.9090921
GTACGAG950.040.2631571
GTTTTTT397850.040.1929132
ACTAACG403.4586265E-739.3751
AACGTAG301.13999224E-437.4999961
TACCGGC301.13999224E-437.49999625
TAACGGG2850.037.105263
ACAACGA1650.036.8181813
CACAACG1650.036.8181812
ACCGGGT504.879621E-836.04
TACGTCC250.00210719536.033
GTTGATC1400.035.35714316
TCGACAC2500.035.10000234
Produced by FastQC (version 0.11.5)