SRR3549874_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302854
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45747	3.51129136495724	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	5567	0.42729269741659465	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	5077	0.389682957568538	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	3002	0.23041722249768584	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1977	0.1517437871012408	Illumina PCR Primer Index 7 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1921	0.14744553111860578	RNA PCR Primer, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1357	0.10415595300778137	Illumina PCR Primer Index 7 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	35	1.2121018E-7	45.000004	3
AATACGG	45	3.8562575E-10	45.000004	2
CTTCGCA	20	7.0336345E-4	45.0	17
CATGATA	20	7.0336345E-4	45.0	9
ATTACGT	25	3.8910934E-5	45.0	11
CCGTATA	20	7.0336345E-4	45.0	41
ATAAGCG	25	3.8910934E-5	45.0	33
CTACCGT	25	3.8910934E-5	45.0	37
TCCGTAT	20	7.0336345E-4	45.0	40
CGCAAAG	20	7.0336345E-4	45.0	22
CCGTTTG	25	3.8910934E-5	45.0	40
CCGCATT	20	7.0336345E-4	45.0	11
TCGTTAC	30	2.165727E-6	44.999996	37
ATTCGTT	30	2.165727E-6	44.999996	13
ACTACGG	60	0.0	44.999996	2
CGTTTTT	31780	0.0	44.518566	1
GCGTTAG	75	0.0	42.0	1
CGGTCTA	60	3.6379788E-12	41.249996	31
CGATGTT	55	6.184564E-11	40.909092	10
GTTTTTT	35520	0.0	40.122467	2