##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302854
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51763359516876	34.0	31.0	34.0	31.0	34.0
2	32.682478620014216	34.0	31.0	34.0	31.0	34.0
3	32.74466977880868	34.0	31.0	34.0	31.0	34.0
4	36.15658546544739	37.0	37.0	37.0	35.0	37.0
5	36.03330457595402	37.0	35.0	37.0	35.0	37.0
6	36.0167171455896	37.0	35.0	37.0	35.0	37.0
7	36.173725528723864	37.0	36.0	37.0	35.0	37.0
8	36.15506879512209	37.0	37.0	37.0	35.0	37.0
9	37.925891926493684	39.0	38.0	39.0	35.0	39.0
10	37.509317237388075	39.0	37.0	39.0	35.0	39.0
11	37.462618988773876	39.0	37.0	39.0	35.0	39.0
12	37.44946402282988	39.0	37.0	39.0	35.0	39.0
13	37.397416748154434	39.0	37.0	39.0	35.0	39.0
14	38.677820385093035	40.0	38.0	41.0	35.0	41.0
15	38.711300729014916	40.0	38.0	41.0	35.0	41.0
16	38.80015182054167	40.0	38.0	41.0	35.0	41.0
17	38.81460854401184	40.0	38.0	41.0	35.0	41.0
18	38.3793456519303	39.0	38.0	41.0	35.0	41.0
19	37.951392097656374	39.0	37.0	41.0	35.0	41.0
20	37.442114005099576	39.0	35.0	41.0	34.0	41.0
21	37.39247452132012	39.0	35.0	41.0	34.0	41.0
22	37.190930833385785	38.0	35.0	41.0	34.0	41.0
23	37.05218543290346	38.0	35.0	40.0	33.0	41.0
24	36.85634921487749	38.0	35.0	40.0	33.0	41.0
25	36.69437404344616	38.0	35.0	40.0	33.0	41.0
26	36.813493300093484	38.0	35.0	40.0	33.0	41.0
27	36.87652568898741	38.0	35.0	40.0	33.0	41.0
28	36.81937807306114	38.0	35.0	40.0	33.0	41.0
29	36.700994892750835	38.0	35.0	40.0	33.0	41.0
30	36.454552850895034	38.0	35.0	40.0	33.0	41.0
31	35.958795843586465	38.0	35.0	40.0	31.0	41.0
32	35.333520870335434	38.0	35.0	40.0	25.0	41.0
33	34.5036028595683	38.0	35.0	40.0	18.0	41.0
34	33.795022312553826	38.0	34.0	40.0	15.0	41.0
35	33.373259014440606	38.0	33.0	41.0	10.0	41.0
36	33.1669411921827	38.0	33.0	41.0	10.0	41.0
37	32.920292680530586	38.0	33.0	40.0	10.0	41.0
38	32.791505418105174	38.0	33.0	40.0	10.0	41.0
39	32.734629513360666	38.0	32.0	40.0	10.0	41.0
40	32.61366200664081	38.0	32.0	40.0	9.0	41.0
41	32.491464124145914	38.0	31.0	40.0	8.0	41.0
42	32.39452233327756	38.0	31.0	40.0	8.0	41.0
43	32.28684948582113	38.0	31.0	40.0	8.0	41.0
44	32.16036869825783	37.0	31.0	40.0	8.0	41.0
45	32.1059074923207	37.0	30.0	40.0	8.0	41.0
46	32.03841336020767	37.0	30.0	40.0	8.0	41.0
47	32.020562549602644	37.0	30.0	40.0	8.0	41.0
48	31.99307443504798	37.0	30.0	40.0	8.0	41.0
49	31.885942707317934	37.0	30.0	40.0	8.0	41.0
50	31.805373434014864	36.0	29.0	40.0	8.0	41.0
51	31.233797493809742	35.0	28.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	10.0
10	14.0
11	14.0
12	7.0
13	18.0
14	24.0
15	57.0
16	124.0
17	208.0
18	402.0
19	801.0
20	1346.0
21	2290.0
22	3497.0
23	6057.0
24	11643.0
25	24778.0
26	45852.0
27	59854.0
28	56921.0
29	44692.0
30	35355.0
31	31654.0
32	31283.0
33	37918.0
34	63771.0
35	86539.0
36	94678.0
37	129391.0
38	237335.0
39	296118.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.01721298011903	16.592342656966935	17.959264814016	34.43117954889804
2	48.81713530449306	19.04357664020681	17.78365035529691	14.355637700003223
3	24.345475394787137	18.47474851364773	43.195937534059844	13.983838557505294
4	22.126961271178505	19.8130412156696	43.20023579004248	14.859761723109418
5	20.034324644204187	22.484560818019517	41.99764516975809	15.483469368018213
6	20.00623247117482	25.612386345668813	42.095814266218625	12.28556691693774
7	60.20774392218928	4.692313950757337	30.672047673799213	4.427894453254163
8	59.34425499710635	5.122676831018672	30.44915239927114	5.083915772603837
9	54.182893862243965	6.605344881314407	32.684015246528006	6.527746009913621
10	28.326274471276136	22.065711123425956	38.15776748584262	11.450246919455289
11	22.863191117346993	19.616779777319636	42.34925786005185	15.170771245281514
12	21.425117472871097	17.18450417314603	45.53196290605087	15.858415447932
13	18.678685409109537	19.58999243199929	46.46529849085162	15.26602366803955
14	15.011966037637373	22.120360378062315	44.93082110505091	17.936852479249403
15	13.941623543390127	20.439819043423128	49.310667196784905	16.307890216401837
16	15.519851034728374	19.89593615247756	47.23990562257935	17.344307190214714
17	15.650947842198743	20.05735101553973	44.633934423964625	19.657766718296905
18	16.131508211971564	20.139171388352032	46.31478277688828	17.414537622788124
19	16.89736532259179	20.703394240644002	44.50122577050076	17.89801466626345
20	18.981558946743075	20.392461473043028	45.026150282380065	15.599829297833834
21	18.2312830140599	21.106278984444916	44.547201758600735	16.115236242894447
22	16.721060072732634	20.168261370805936	43.56597132142205	19.544707235039382
23	15.851507536531336	21.27483202262111	44.70224599226007	18.171414448587488
24	16.635785744219998	19.339158493584087	45.7135642213172	18.311491540878716
25	15.954895943827935	21.18456864698577	44.25891158947971	18.601623819706585
26	15.191341470341266	22.93065838536014	43.89348307638461	17.98451706791398
27	17.855339124721574	21.61876925580303	44.04607116376816	16.47982045570724
28	14.499168747994787	21.257178471263856	45.961865258885496	18.281787521855865
29	15.433809160504556	20.90119077041633	44.214240429088754	19.45075963999036
30	17.006894095577863	20.933427690286095	43.05578368719749	19.003894526938552
31	16.92952548789043	22.948542200430747	41.167851501396164	18.954080810282655
32	17.020249390952475	23.477227686294857	39.04758322881919	20.454939693933472
33	17.022398518943795	23.01017612103889	37.83455398686269	22.13287137315463
34	15.630454371710107	24.82304233628634	38.26737301339981	21.27913027860374
35	17.142519422744222	24.357295598739384	34.78048960205825	23.719695376458144
36	18.777315032996793	26.492607767255578	33.450563148288296	21.279514051459334
37	18.018826361203942	26.779976881523176	35.25821005269969	19.94298670457319
38	16.88255169036592	25.984262242737866	33.6283267349987	23.504859331897514
39	17.987894269043192	25.404611721651083	33.309641755714765	23.29785225359096
40	19.25541925649382	25.44567541719947	34.003119305770255	21.295786020536454
41	16.7093166233515	26.765393513010665	32.434255872108466	24.091033991529365
42	18.261908087936177	25.588055146624257	32.822864265681346	23.327172499758223
43	19.625069271000434	24.828798929120225	33.17777740253321	22.368354397346135
44	18.14301525727365	25.329852769381684	33.26543112275051	23.26170085059416
45	18.006852648109458	24.72333814840343	32.6038067197092	24.66600248377792
46	18.14746702239852	25.839119348752813	33.401823995628064	22.611589633220603
47	16.60677251633721	24.966266365993427	36.21879351024751	22.20816760742186
48	17.12179568854223	22.732324573589977	35.759647665816736	24.38623207205105
49	17.505568544134643	22.17984517067914	36.96415715037909	23.350429134807126
50	16.898593395729684	22.602532593828624	36.44046071163768	24.05841329880401
51	16.469765606890718	22.159965736759453	34.23353652826794	27.136732128081885
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	417.0
1	803.5
2	1190.0
3	46227.0
4	91264.0
5	63547.0
6	35830.0
7	34477.5
8	33125.0
9	31741.0
10	30357.0
11	28676.0
12	26995.0
13	25196.0
14	23397.0
15	21208.0
16	19019.0
17	17364.5
18	15710.0
19	14438.5
20	13167.0
21	12282.0
22	11397.0
23	10532.5
24	9668.0
25	10367.0
26	12111.5
27	13157.0
28	14145.0
29	15133.0
30	16568.0
31	18003.0
32	20284.5
33	22566.0
34	24473.5
35	26381.0
36	27540.0
37	28699.0
38	31782.0
39	34865.0
40	38188.0
41	41511.0
42	45221.5
43	48932.0
44	54258.5
45	59585.0
46	63681.0
47	67777.0
48	78030.0
49	88283.0
50	99733.5
51	111184.0
52	110650.5
53	110117.0
54	91987.0
55	73857.0
56	62884.5
57	51912.0
58	47353.5
59	42795.0
60	40173.5
61	37552.0
62	33510.0
63	29468.0
64	24909.0
65	20350.0
66	16581.5
67	12813.0
68	11499.0
69	10185.0
70	7797.0
71	5409.0
72	4728.0
73	4047.0
74	3369.0
75	2143.5
76	1596.0
77	1190.0
78	784.0
79	568.5
80	353.0
81	237.0
82	121.0
83	73.0
84	25.0
85	55.5
86	86.0
87	47.5
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1302854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.64072538822006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35123442619329	24.595189505687685
2	10.493280484841078	6.850165734545325
3	4.206338643719122	4.118938336784817
4	2.099676914271987	2.7413991025094875
5	1.2527409404305787	2.0445186509587527
6	0.8077478922932338	1.58192862811542
7	0.584022445788866	1.3344041391485708
8	0.4346591469372644	1.135007188212579
9	0.3266600111187748	0.9596177746416754
>10	3.708231472615858	29.946647909133784
>50	0.6411783847812103	13.095188658148302
>100	0.08679639644900343	5.05214821995181
>500	0.00527491910690502	1.3153554387863329
>1k	0.001438614301883187	0.8161981240615614
>5k	4.79538100627729E-4	0.8330245308765232
>10k+	2.397690503138645E-4	3.5802680584374587
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45747	3.51129136495724	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	5567	0.42729269741659465	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	5077	0.389682957568538	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	3002	0.23041722249768584	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1977	0.1517437871012408	Illumina PCR Primer Index 7 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1921	0.14744553111860578	RNA PCR Primer, Index 16 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1357	0.10415595300778137	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24668919157480423	0.0
2	0.0	0.0	0.0	1.3981612674942856	0.0
3	0.0	0.0	0.0	2.0020662330545096	0.0
4	0.0	0.0	0.0	2.563602675357331	0.0
5	0.0	0.0	0.0	4.035678594838716	0.0
6	0.0	0.0	0.0	4.776129942418721	0.0
7	0.0	0.0	0.0	5.54367565360355	0.0
8	0.0	0.0	0.0	6.756014104419989	0.0
9	0.0	0.0	0.0	7.242177557884459	0.0
10	0.0	0.0	0.0	8.34867145512851	0.0
11	0.0	0.0	0.0	9.887600606054093	0.0
12	0.0	0.0	0.0	11.194577443059622	0.0
13	0.0	0.0	0.0	11.69455671932542	0.0
14	0.0	0.0	0.0	11.906629599325788	0.0
15	0.0	0.0	0.0	12.32440472992369	0.0
16	0.0	0.0	0.0	13.143529512900141	0.0
17	0.0	0.0	0.0	14.077402379698723	0.0
18	0.0	0.0	0.0	15.11474040836502	0.0
19	0.0	0.0	0.0	15.702757177703718	0.0
20	0.0	0.0	0.0	16.269129157987003	0.0
21	0.0	0.0	0.0	16.94978869466571	0.0
22	0.0	0.0	0.0	17.693310225090457	0.0
23	0.0	0.0	0.0	18.3953075325401	0.0
24	7.675457111848297E-5	0.0	0.0	18.942644379186003	0.0
25	7.675457111848297E-5	0.0	0.0	19.436176271477848	0.0
26	7.675457111848297E-5	0.0	0.0	19.88833744993683	0.0
27	7.675457111848297E-5	0.0	0.0	20.329599479296988	0.0
28	7.675457111848297E-5	0.0	0.0	20.773624673217412	0.0
29	1.5350914223696594E-4	0.0	0.0	21.242211329895753	0.0
30	1.5350914223696594E-4	0.0	0.0	21.766905578061703	0.0
31	1.5350914223696594E-4	0.0	0.0	22.254373859235187	0.0
32	1.5350914223696594E-4	0.0	0.0	22.70461617341621	0.0
33	1.5350914223696594E-4	0.0	0.0	23.12354262258089	0.0
34	1.5350914223696594E-4	0.0	0.0	23.54339012659899	0.0
35	2.3026371335544888E-4	0.0	0.0	24.01497021155095	0.0
36	3.0701828447393187E-4	0.0	0.0	24.463370416025125	0.0
37	3.0701828447393187E-4	0.0	0.0	24.903250863105153	0.0
38	3.0701828447393187E-4	0.0	0.0	25.305368061194883	0.0
39	3.0701828447393187E-4	0.0	0.0	25.723066437221668	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	35	1.2121018E-7	45.000004	3
AATACGG	45	3.8562575E-10	45.000004	2
CTTCGCA	20	7.0336345E-4	45.0	17
CATGATA	20	7.0336345E-4	45.0	9
ATTACGT	25	3.8910934E-5	45.0	11
CCGTATA	20	7.0336345E-4	45.0	41
ATAAGCG	25	3.8910934E-5	45.0	33
CTACCGT	25	3.8910934E-5	45.0	37
TCCGTAT	20	7.0336345E-4	45.0	40
CGCAAAG	20	7.0336345E-4	45.0	22
CCGTTTG	25	3.8910934E-5	45.0	40
CCGCATT	20	7.0336345E-4	45.0	11
TCGTTAC	30	2.165727E-6	44.999996	37
ATTCGTT	30	2.165727E-6	44.999996	13
ACTACGG	60	0.0	44.999996	2
CGTTTTT	31780	0.0	44.518566	1
GCGTTAG	75	0.0	42.0	1
CGGTCTA	60	3.6379788E-12	41.249996	31
CGATGTT	55	6.184564E-11	40.909092	10
GTTTTTT	35520	0.0	40.122467	2
>>END_MODULE