FastQCFastQC Report
Sat 18 Jun 2016
SRR3549872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713243
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289054.052616008849719No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC34250.4802009974160279No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC23100.3238727894981093No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT14660.20554004736113776Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG12210.17118990302042922No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC9120.1278666597499029No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC8030.11258435063505705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT650.045.00000414
CCGATAC207.031484E-445.011
TACGGAC207.031484E-445.038
TATTGCG207.031484E-445.01
CACTCGA207.031484E-445.038
CGAGCTA207.031484E-445.07
CAGCTTA207.031484E-445.031
ACGTCCA207.031484E-445.029
TTACGCG207.031484E-445.01
GTAAGCG207.031484E-445.01
TTCGCGC207.031484E-445.031
TTACCGA207.031484E-445.036
GATTACC207.031484E-445.034
ACGGTAG453.8380676E-1045.01
TAGGTCG253.889313E-544.9999961
TATAGCG600.044.9999961
CGTAAAT253.889313E-544.99999628
TGTAGCG302.164339E-644.9999961
CGTTTTT206950.044.554241
AAGCTAC750.042.0000048