##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713243
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.612338291437844	34.0	31.0	34.0	31.0	34.0
2	32.772838990358125	34.0	31.0	34.0	31.0	34.0
3	32.81575844417681	34.0	31.0	34.0	31.0	34.0
4	36.20630556486359	37.0	37.0	37.0	35.0	37.0
5	36.0920948400475	37.0	35.0	37.0	35.0	37.0
6	36.08566925998573	37.0	36.0	37.0	35.0	37.0
7	36.228463511033404	37.0	37.0	37.0	35.0	37.0
8	36.22716942192212	37.0	37.0	37.0	35.0	37.0
9	38.00396919422974	39.0	39.0	39.0	35.0	39.0
10	37.6689052679101	39.0	38.0	39.0	35.0	39.0
11	37.570261467690536	39.0	37.0	39.0	35.0	39.0
12	37.44423709731466	39.0	37.0	39.0	35.0	39.0
13	37.36253142337184	39.0	37.0	39.0	34.0	39.0
14	38.65973027425436	40.0	38.0	41.0	34.0	41.0
15	38.73131176892027	40.0	38.0	41.0	35.0	41.0
16	38.82564707960681	40.0	38.0	41.0	35.0	41.0
17	38.83786451461844	40.0	38.0	41.0	35.0	41.0
18	38.31637604575159	39.0	38.0	40.0	35.0	41.0
19	37.78788435357936	38.0	37.0	40.0	35.0	41.0
20	37.19787646005639	38.0	35.0	40.0	34.0	41.0
21	37.126081854290895	38.0	35.0	40.0	34.0	41.0
22	36.89531898665672	37.0	35.0	40.0	34.0	41.0
23	36.813775950132005	37.0	35.0	40.0	33.0	41.0
24	36.62418418407191	37.0	35.0	40.0	33.0	41.0
25	36.45249935856363	36.0	35.0	40.0	33.0	41.0
26	36.55200541750848	37.0	35.0	40.0	33.0	41.0
27	36.59429815644879	37.0	35.0	40.0	33.0	41.0
28	36.534083054442874	37.0	35.0	40.0	33.0	41.0
29	36.37334681167568	37.0	35.0	40.0	33.0	41.0
30	36.08653993099126	37.0	35.0	40.0	32.0	41.0
31	35.48294199872974	37.0	35.0	40.0	30.0	41.0
32	34.678199435535994	37.0	35.0	40.0	21.0	41.0
33	33.637934056135144	37.0	34.0	40.0	15.0	41.0
34	32.747581118917395	37.0	33.0	40.0	10.0	41.0
35	32.23225184123784	37.0	31.0	40.0	8.0	41.0
36	31.976920909143168	37.0	31.0	40.0	8.0	41.0
37	31.705260339042933	36.0	30.0	40.0	7.0	41.0
38	31.577766623717302	36.0	29.0	40.0	7.0	41.0
39	31.52665921712516	36.0	29.0	40.0	7.0	41.0
40	31.369656624740795	36.0	27.0	40.0	7.0	41.0
41	31.206963124769537	36.0	26.0	40.0	7.0	41.0
42	31.05734230830166	36.0	25.0	40.0	7.0	41.0
43	30.937025670073172	35.0	24.0	40.0	7.0	41.0
44	30.813135214786545	35.0	23.0	40.0	7.0	41.0
45	30.805785685944343	35.0	23.0	40.0	7.0	41.0
46	30.79120299813668	35.0	23.0	40.0	7.0	41.0
47	30.762591430970932	35.0	23.0	40.0	7.0	41.0
48	30.710091791997957	35.0	23.0	40.0	7.0	41.0
49	30.58064642765509	35.0	24.0	40.0	7.0	41.0
50	30.49417099081239	35.0	24.0	40.0	7.0	41.0
51	29.930874891166123	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	4.0
12	7.0
13	12.0
14	19.0
15	26.0
16	58.0
17	125.0
18	240.0
19	476.0
20	854.0
21	1327.0
22	2230.0
23	3921.0
24	7826.0
25	17119.0
26	30878.0
27	39387.0
28	35488.0
29	27361.0
30	21584.0
31	18840.0
32	18009.0
33	21518.0
34	38728.0
35	53438.0
36	50167.0
37	63849.0
38	116257.0
39	143390.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.188349552677	15.218095375629343	18.10547036563976	39.4880847060539
2	51.967982861381046	17.832071257621877	17.858289531057437	12.341656349939642
3	22.4124176472815	17.343317775288366	47.5403193581991	12.703945219231032
4	21.421170624878197	18.13449273249089	46.871963692598456	13.572372950032458
5	18.710733929390123	20.994808221041076	46.5236952903849	13.770762559183897
6	19.132048965079225	24.236200004766957	45.11267548367106	11.519075546482755
7	56.02017825621842	4.867205146072236	34.87030366929644	4.242312928412898
8	55.64036380307974	4.8090202076991995	34.716218736110974	4.83439725311009
9	51.423708329419284	6.541669529178695	36.57252857721702	5.462093564184997
10	29.082654859563988	19.98267070269179	41.09819514527307	9.83647929247115
11	24.140019600612973	18.25576977271421	44.24032202208784	13.363888604584972
12	21.90515714840524	16.285333329594543	48.372293874598135	13.437215647402079
13	18.319142283906046	19.645478469469733	49.03111562258585	13.00426362403837
14	13.68369545863051	22.38353548510115	48.009163777282076	15.923605278986264
15	13.123998412883125	19.611128325129023	52.9881120459647	14.276761216023152
16	14.532774944864514	18.356857340345435	51.790063134163255	15.320304580626798
17	14.504734010708834	18.62759255961853	47.74389654016934	19.123776889503297
18	15.84228656993479	18.856967401012	49.5092135499402	15.79153247911301
19	17.205496583913195	19.467278332910382	47.724127681589586	15.603097401586835
20	18.691245480151924	19.562196895027363	47.43895138122631	14.307606243594398
21	17.71135503608167	19.446528041635176	47.976496088990714	14.86562083329244
22	15.638849592635328	19.670575105539065	46.726571449001256	17.964003852824355
23	15.28959975772633	20.033004179501233	47.679402391611276	16.99799367116116
24	16.720108013678367	17.923484702969393	49.07682234525961	16.27958493809263
25	15.117007807998114	19.72371267576408	47.414415563840095	17.74486395239771
26	14.193339436910001	22.3812922103687	47.18055417298172	16.244814179739585
27	16.073484072048377	21.283910252186143	47.98967532804388	14.652930347721604
28	14.071221168662012	19.536118826262577	50.227622283008735	16.16503772206667
29	14.191236366848326	20.301916738054214	48.988493402669214	16.518353492428247
30	17.27013093714204	20.11165899980792	46.85836383953295	15.75984622351709
31	17.393511047427033	20.706968031933016	45.65624899227893	16.243271928361022
32	18.566463323159148	22.016339452332513	43.94168607332985	15.47551115117849
33	20.18358399591724	22.431064868495028	40.76913478295616	16.61621635263157
34	17.186849362699668	23.481197852625264	41.5517572552412	17.78019552943387
35	18.96380336014514	24.046923699216116	38.05365071932007	18.93562222131868
36	21.52688494664511	25.2609559434863	36.47536674036759	16.736792369500996
37	19.207198668616446	25.944593918201793	38.64068767586923	16.207519737312527
38	19.24982088853308	26.352729714837718	35.956048639804386	18.441400756824812
39	20.413239246652264	25.324188250007364	36.74708339233613	17.515489111004243
40	20.880681619027456	24.588394137762307	36.855742012189395	17.675182231020845
41	18.80929781294734	25.741437350243885	35.403642236937486	20.045622599871294
42	19.78974907570071	26.086761454371093	34.73724943672774	19.386240033200465
43	21.641291958000288	24.20633640989116	35.30297528331859	18.849396348789963
44	18.74536448307239	25.287314421592637	35.616613131849874	20.350707963485096
45	18.64105220801326	24.69242600347988	35.26105408675584	21.405467701751014
46	19.949862809729645	24.95839426394651	36.00960682404173	19.08213610228211
47	18.225485563826073	24.31681769046454	38.99428385557236	18.463412890137022
48	19.03586856092524	22.632118366391257	37.971070168231584	20.36094290445192
49	18.58539095371423	22.095134477309976	39.24104407614235	20.078430492833437
50	17.633120829787323	22.683152866554597	39.069012945097256	20.614713358560827
51	17.286955497635446	22.359139872385708	36.51728793693033	23.836616693048512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432.0
1	617.5
2	803.0
3	29726.0
4	58649.0
5	40711.5
6	22774.0
7	21674.0
8	20574.0
9	19939.5
10	19305.0
11	18702.0
12	18099.0
13	16986.0
14	15873.0
15	14499.0
16	13125.0
17	11974.5
18	10824.0
19	9920.0
20	9016.0
21	8422.0
22	7828.0
23	6930.5
24	6033.0
25	6334.0
26	6937.0
27	7239.0
28	7745.5
29	8252.0
30	9191.5
31	10131.0
32	10787.0
33	11443.0
34	12197.5
35	12952.0
36	13689.0
37	14426.0
38	15662.0
39	16898.0
40	20991.0
41	25084.0
42	28556.0
43	32028.0
44	35535.0
45	39042.0
46	43345.5
47	47649.0
48	51593.5
49	55538.0
50	54229.5
51	52921.0
52	44314.0
53	35707.0
54	31363.0
55	27019.0
56	24862.5
57	22706.0
58	21687.5
59	20669.0
60	19145.0
61	17621.0
62	16512.0
63	15403.0
64	13222.0
65	11041.0
66	8861.0
67	6681.0
68	5773.0
69	4865.0
70	4107.5
71	3350.0
72	2754.0
73	2158.0
74	1676.0
75	924.5
76	655.0
77	489.0
78	323.0
79	223.5
80	124.0
81	120.0
82	116.0
83	69.0
84	22.0
85	14.5
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.74276820238458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64688026715628	27.753144430272762
2	9.959274718950685	7.119440954866464
3	3.7645880393363447	4.036703930024052
4	1.8888005223027224	2.700438369968366
5	1.0754026691393448	1.9218934163636638
6	0.6647631458679867	1.4256285019348451
7	0.4601526022576068	1.1512989460152396
8	0.33897724764572923	0.9692788150786893
9	0.28426363922397196	0.9144332428633838
>10	3.317128531990343	29.704569554149113
>50	0.5210514827026121	11.757287612733553
>100	0.07352260177892778	4.451436311098977
>500	0.0031966348599533814	0.7633767672877321
>1k	0.0015983174299766907	1.2028361336009878
>5k	0.0	0.0
>10k+	3.9957935749417267E-4	4.12823301374217
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28905	4.052616008849719	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	3425	0.4802009974160279	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2310	0.3238727894981093	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1466	0.20554004736113776	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1221	0.17118990302042922	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	912	0.1278666597499029	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	803	0.11258435063505705	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.402046707784023E-4	0.0	0.0	0.21647601168185318	0.0
2	1.402046707784023E-4	0.0	0.0	1.307969373691715	0.0
3	1.402046707784023E-4	0.0	0.0	1.855749022422933	0.0
4	1.402046707784023E-4	0.0	0.0	2.3391747272668644	0.0
5	1.402046707784023E-4	0.0	0.0	3.644900826226125	0.0
6	1.402046707784023E-4	0.0	0.0	4.276382663412049	0.0
7	1.402046707784023E-4	0.0	0.0	4.961282480164544	0.0
8	1.402046707784023E-4	0.0	0.0	5.957997484728207	0.0
9	1.402046707784023E-4	0.0	0.0	6.344962376076596	0.0
10	1.402046707784023E-4	0.0	0.0	7.215072562927362	0.0
11	1.402046707784023E-4	0.0	0.0	8.461632290818136	0.0
12	1.402046707784023E-4	0.0	0.0	9.49606235182119	0.0
13	1.402046707784023E-4	0.0	0.0	9.915134112777833	0.0
14	1.402046707784023E-4	0.0	0.0	10.08926831388461	0.0
15	1.402046707784023E-4	0.0	0.0	10.409215372600922	0.0
16	1.402046707784023E-4	0.0	0.0	11.07294428406588	0.0
17	1.402046707784023E-4	0.0	0.0	11.876457252296904	0.0
18	4.2061401233520695E-4	0.0	0.0	12.854104421634702	0.0
19	4.2061401233520695E-4	0.0	0.0	13.34664343007923	0.0
20	4.2061401233520695E-4	0.0	0.0	13.855446180334052	0.0
21	4.2061401233520695E-4	0.0	0.0	14.459027288035074	0.0
22	4.2061401233520695E-4	0.0	0.0	15.094995674685906	0.0
23	4.2061401233520695E-4	0.0	0.0	15.707830290658302	0.0
24	4.2061401233520695E-4	0.0	0.0	16.212987719472885	0.0
25	4.2061401233520695E-4	0.0	0.0	16.63752746258989	0.0
26	4.2061401233520695E-4	0.0	0.0	17.04103650509013	0.0
27	4.2061401233520695E-4	0.0	0.0	17.437395109380674	0.0
28	4.2061401233520695E-4	0.0	0.0	17.834174327683552	0.0
29	4.2061401233520695E-4	0.0	0.0	18.25759243343433	0.0
30	4.2061401233520695E-4	0.0	0.0	18.713257613464133	0.0
31	4.2061401233520695E-4	0.0	0.0	19.150976595634308	0.0
32	4.2061401233520695E-4	0.0	0.0	19.57972247887466	0.0
33	4.2061401233520695E-4	0.0	0.0	19.978604767239215	0.0
34	4.2061401233520695E-4	0.0	0.0	20.396274481488078	0.0
35	4.2061401233520695E-4	0.0	0.0	20.814785423761606	0.0
36	4.2061401233520695E-4	0.0	0.0	21.227688179204	0.0
37	4.2061401233520695E-4	0.0	0.0	21.62909415164257	0.0
38	4.2061401233520695E-4	0.0	0.0	22.011993107538384	0.0
39	4.2061401233520695E-4	0.0	0.0	22.39503226810498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	65	0.0	45.000004	14
CCGATAC	20	7.031484E-4	45.0	11
TACGGAC	20	7.031484E-4	45.0	38
TATTGCG	20	7.031484E-4	45.0	1
CACTCGA	20	7.031484E-4	45.0	38
CGAGCTA	20	7.031484E-4	45.0	7
CAGCTTA	20	7.031484E-4	45.0	31
ACGTCCA	20	7.031484E-4	45.0	29
TTACGCG	20	7.031484E-4	45.0	1
GTAAGCG	20	7.031484E-4	45.0	1
TTCGCGC	20	7.031484E-4	45.0	31
TTACCGA	20	7.031484E-4	45.0	36
GATTACC	20	7.031484E-4	45.0	34
ACGGTAG	45	3.8380676E-10	45.0	1
TAGGTCG	25	3.889313E-5	44.999996	1
TATAGCG	60	0.0	44.999996	1
CGTAAAT	25	3.889313E-5	44.999996	28
TGTAGCG	30	2.164339E-6	44.999996	1
CGTTTTT	20695	0.0	44.55424	1
AAGCTAC	75	0.0	42.000004	8
>>END_MODULE