FastQCFastQC Report
Sat 18 Jun 2016
SRR3549870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217933
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480883.9483288489596715No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC36140.2967322504604112No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC34460.28293838823646295No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT18480.15173248446343107Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGT302.1656087E-645.00000411
TAGGCCG302.1656087E-645.0000041
TACACGG302.1656087E-645.0000042
CGAACGT207.033453E-445.037
ATTAGCG700.045.01
CACGAAC253.8909442E-545.043
TAACGAC253.8909442E-545.010
TTATCCG253.8909442E-545.01
CTGCGTA207.033453E-445.024
GCCGATT207.033453E-445.09
TGCGATA253.8909442E-545.014
CATAGCG207.033453E-445.01
CTACACG207.033453E-445.027
CGGTCTA207.033453E-445.031
CCGCAAT207.033453E-445.033
GATAGTC207.033453E-445.040
CGTTTTT330050.044.3660051
TCTAGCG556.184564E-1140.9090921
TTCGACG556.184564E-1140.90909225
GTACGAG556.184564E-1140.9090921