##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1217933
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.560204871696556	34.0	31.0	34.0	31.0	34.0
2	32.71582098522661	34.0	31.0	34.0	31.0	34.0
3	32.76744779885264	34.0	31.0	34.0	31.0	34.0
4	36.178712622122895	37.0	37.0	37.0	35.0	37.0
5	36.062280109004355	37.0	35.0	37.0	35.0	37.0
6	36.048153716173225	37.0	35.0	37.0	35.0	37.0
7	36.19878433378519	37.0	37.0	37.0	35.0	37.0
8	36.19157539864673	37.0	37.0	37.0	35.0	37.0
9	37.93806227436156	39.0	39.0	39.0	35.0	39.0
10	37.571966602432155	39.0	37.0	39.0	35.0	39.0
11	37.50034936240335	39.0	37.0	39.0	35.0	39.0
12	37.450743185380475	39.0	37.0	39.0	35.0	39.0
13	37.43058936739541	39.0	37.0	39.0	35.0	39.0
14	38.739293540777695	40.0	38.0	41.0	35.0	41.0
15	38.7838107679158	40.0	38.0	41.0	35.0	41.0
16	38.86544580038475	40.0	38.0	41.0	35.0	41.0
17	38.889454510223466	40.0	38.0	41.0	35.0	41.0
18	38.39606530080062	39.0	38.0	41.0	35.0	41.0
19	37.90117682992414	38.0	37.0	41.0	35.0	41.0
20	37.33365628486953	38.0	35.0	41.0	34.0	41.0
21	37.26618787732987	38.0	35.0	41.0	34.0	41.0
22	37.095021647332	38.0	35.0	40.0	34.0	41.0
23	36.98172559574295	38.0	35.0	40.0	33.0	41.0
24	36.773354527712115	38.0	35.0	40.0	33.0	41.0
25	36.60602019979753	37.0	35.0	40.0	33.0	41.0
26	36.74627914671825	38.0	35.0	40.0	33.0	41.0
27	36.78750144712394	38.0	35.0	40.0	33.0	41.0
28	36.71455244253994	38.0	35.0	40.0	33.0	41.0
29	36.582605118672376	38.0	35.0	40.0	33.0	41.0
30	36.28141367382278	38.0	35.0	40.0	33.0	41.0
31	35.783139138195615	38.0	35.0	40.0	30.0	41.0
32	35.049152129058	38.0	35.0	40.0	23.0	41.0
33	34.068709855139815	38.0	34.0	40.0	16.0	41.0
34	33.29203494773522	38.0	33.0	40.0	12.0	41.0
35	32.80465674220174	38.0	33.0	40.0	10.0	41.0
36	32.52911695470933	38.0	32.0	40.0	8.0	41.0
37	32.31398032568294	38.0	31.0	40.0	8.0	41.0
38	32.15939218331386	37.0	31.0	40.0	8.0	41.0
39	32.06457498072554	37.0	30.0	40.0	8.0	41.0
40	31.936984218343703	37.0	30.0	40.0	8.0	41.0
41	31.797760632153	37.0	29.0	40.0	7.0	41.0
42	31.642968045040245	37.0	29.0	40.0	7.0	41.0
43	31.504118863681335	37.0	27.0	40.0	7.0	41.0
44	31.402119821041058	37.0	27.0	40.0	7.0	41.0
45	31.38911746376853	37.0	26.0	40.0	7.0	41.0
46	31.334708887927334	36.0	26.0	40.0	7.0	41.0
47	31.312927722625137	36.0	26.0	40.0	7.0	41.0
48	31.246940513148097	36.0	26.0	40.0	7.0	41.0
49	31.11214984732329	36.0	26.0	40.0	7.0	41.0
50	31.00906453803288	35.0	26.0	40.0	7.0	41.0
51	30.459254326797943	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	13.0
11	6.0
12	16.0
13	19.0
14	33.0
15	55.0
16	116.0
17	266.0
18	431.0
19	874.0
20	1469.0
21	2286.0
22	3659.0
23	6337.0
24	12176.0
25	26006.0
26	47724.0
27	62683.0
28	58569.0
29	46260.0
30	35280.0
31	30590.0
32	30107.0
33	35752.0
34	59408.0
35	79481.0
36	86832.0
37	113778.0
38	208225.0
39	269334.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.738718796518366	15.48090083773081	17.129924224074724	36.6504561416761
2	50.98966856140691	17.8958120027949	18.40643122404927	12.708088211748922
3	23.59243078231725	18.13392033880353	45.32810918170376	12.945539697175459
4	21.951289602958454	19.10088650196686	45.118327527047875	13.829496368026811
5	19.245804161641075	22.277087491676472	43.99650883915618	14.480599507526277
6	19.982051557844315	25.328897402402266	43.32003484592338	11.369016193830038
7	58.21904817424275	5.13131674730876	32.648265544984824	4.001369533463663
8	58.04605015218407	4.929088874346947	32.638987530512765	4.385873442956221
9	52.84371143568652	7.160574514361627	34.3632203085063	5.632493741445548
10	28.56314756230433	21.03843150649502	39.5769718038677	10.821449127332947
11	23.382813340306896	18.926000034484655	43.81858443773179	13.872602187476652
12	21.673195487764925	16.70912931992154	47.03493541927183	14.582739773041704
13	18.442804325032657	18.707104578002237	47.7501635968481	15.099927500117003
14	14.661890268183884	21.212825336040652	47.02434370363559	17.10094069213988
15	14.023595715035228	19.968668227234172	50.77890163087789	15.228834426852709
16	15.323092485383022	19.146948149036113	50.22690082295167	15.303058542629191
17	15.977644090438472	18.86959299074744	47.060798910941735	18.091964007872356
18	16.093742430823372	19.329552610857903	47.66304878839805	16.913656169920678
19	17.781520001510756	20.304400981006342	46.30147963804249	15.612599379440411
20	19.907088485162976	19.411658933619503	46.54123010050635	14.140022480711172
21	18.178504072063078	19.823175823300627	46.53154155442048	15.466778550215817
22	16.711346190636103	19.52939940045963	44.963885533933315	18.795368874970954
23	16.677928917272133	20.21777881049286	46.12191311016288	16.982379162072135
24	17.23304976546329	18.627789870214535	47.32608443978445	16.81307592453772
25	16.372164971307946	19.96940718413903	45.98832612302976	17.670101721523267
26	16.353526836041063	20.998363620987362	45.836675744889085	16.81143379808249
27	16.921127845291984	20.980546548948094	45.88897747248822	16.209348133271696
28	15.148534443191867	20.753604672835042	47.49604452790096	16.60181635607213
29	17.01357956472154	19.584739062000946	46.362566742177115	17.039114631100396
30	16.610191200993814	20.634139973216918	44.94163472046492	17.81403410532435
31	18.037034877944848	21.41415003945209	42.92970138751475	17.619113695088316
32	19.54105849829178	22.309683701812823	41.22394253214257	16.925315267752826
33	18.291728691151317	23.887849331613477	40.05827906789618	17.76214290933902
34	18.876736240827697	23.75935293649158	39.26078035491279	18.10313046776793
35	20.31080527418175	23.299803847994923	36.059947468374695	20.32944340944863
36	19.399342985205262	25.91792816189396	36.017170074215905	18.665558778684872
37	20.092320349313137	26.096345201254913	36.454057817630364	17.357276631801586
38	19.51650870778606	26.425837874497198	35.07754531653219	18.98010810118455
39	20.669199373036122	25.455587458423413	35.427564570464874	18.44764859807559
40	21.517357687163415	25.53736535589396	35.522890011191095	17.42238694575153
41	19.111724536571387	25.98927855637379	34.82728524475484	20.07171166229998
42	20.0697411105537	25.595003994472602	34.66816319124287	19.66709170373083
43	20.542345104369453	25.330046890920926	34.55920810093823	19.56839990377139
44	19.071410332095443	26.718218489851246	34.04152773592636	20.16884344212695
45	19.55403129728811	25.28809055998975	33.38484136647911	21.77303677624303
46	19.50099061278412	26.136905724699144	34.263214807382674	20.098888855134067
47	19.156800907767504	24.562106454131712	37.42907040042432	18.852022237676458
48	19.049898475531904	22.952411996390605	37.04095381273026	20.956735715347232
49	18.693557034746576	23.11916993791941	38.049055243597145	20.138217783736874
50	17.8650221317593	23.295698531856843	37.62234868420513	21.216930652178732
51	17.685209284911405	23.24569578129503	35.835222462976205	23.23387247081736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	340.0
1	771.5
2	1203.0
3	48221.5
4	95240.0
5	66590.5
6	37941.0
7	36092.5
8	34244.0
9	32845.5
10	31447.0
11	30090.0
12	28733.0
13	27004.0
14	25275.0
15	22711.0
16	20147.0
17	18588.5
18	17030.0
19	15595.5
20	14161.0
21	13059.0
22	11957.0
23	11060.5
24	10164.0
25	10667.0
26	11915.0
27	12660.0
28	14508.5
29	16357.0
30	16938.5
31	17520.0
32	19830.5
33	22141.0
34	22668.0
35	23195.0
36	25348.0
37	27501.0
38	29414.5
39	31328.0
40	34557.0
41	37786.0
42	42181.0
43	46576.0
44	50684.5
45	54793.0
46	58190.0
47	61587.0
48	67319.5
49	73052.0
50	80272.5
51	87493.0
52	86562.5
53	85632.0
54	73294.5
55	60957.0
56	53932.5
57	46908.0
58	44885.0
59	42862.0
60	42306.5
61	41751.0
62	36594.5
63	31438.0
64	25893.0
65	20348.0
66	16680.0
67	13012.0
68	11044.0
69	9076.0
70	7254.0
71	5432.0
72	4834.5
73	4237.0
74	3297.5
75	1960.0
76	1562.0
77	1138.5
78	715.0
79	541.0
80	367.0
81	257.5
82	148.0
83	105.5
84	63.0
85	39.0
86	15.0
87	10.0
88	5.0
89	5.0
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1217933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.39782417401355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72404951950624	25.958141399447914
2	9.61257709468325	6.420783193347623
3	3.647958393544658	3.6550161906516414
4	1.803294285227894	2.4090442188817853
5	1.0757464231365699	1.796379494786957
6	0.6602071324793143	1.3229689037384287
7	0.4959055483674054	1.1593516417904663
8	0.37133292382247557	0.9921369359876324
9	0.29785038473182474	0.8952799301492195
>10	3.5517355101719876	30.0050527472687
>50	0.6665219681394121	14.058430176866029
>100	0.08606566245297391	5.059204991196648
>500	0.0045034358240413205	1.0398831667766153
>1k	0.0020015270329072537	1.210175002364461
>5k	0.0	0.0
>10k+	2.501908791134067E-4	4.018152006745993
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48088	3.9483288489596715	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3614	0.2967322504604112	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3446	0.28293838823646295	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1848	0.15173248446343107	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16273473171348507	0.0
2	0.0	0.0	0.0	0.960397657342399	0.0
3	0.0	0.0	0.0	1.3701903142455292	0.0
4	0.0	0.0	0.0	1.7767808245609569	0.0
5	0.0	0.0	0.0	2.8711760006502822	0.0
6	0.0	0.0	0.0	3.4202209809570805	0.0
7	0.0	0.0	0.0	3.982074547614688	0.0
8	0.0	0.0	0.0	4.841974065896893	0.0
9	0.0	0.0	0.0	5.176393118504877	0.0
10	0.0	0.0	0.0	5.946878851299702	0.0
11	0.0	0.0	0.0	7.027808590456125	0.0
12	0.0	0.0	0.0	7.927201249986657	0.0
13	0.0	0.0	0.0	8.271801486617079	0.0
14	0.0	0.0	0.0	8.42238448256185	0.0
15	0.0	0.0	0.0	8.715421948497989	0.0
16	0.0	0.0	0.0	9.331958326114819	0.0
17	0.0	0.0	0.0	10.02197986260328	0.0
18	0.0	0.0	0.0	10.83639247807556	0.0
19	0.0	0.0	0.0	11.288962529137482	0.0
20	8.21063227615969E-5	0.0	0.0	11.74243574974978	0.0
21	1.642126455231938E-4	0.0	0.0	12.30379667847082	0.0
22	1.642126455231938E-4	0.0	0.0	12.876898811346765	0.0
23	2.463189682847907E-4	0.0	0.0	13.474468628405667	0.0
24	2.463189682847907E-4	0.0	0.0	13.921291236874278	0.0
25	2.463189682847907E-4	0.0	0.0	14.319589008590784	0.0
26	2.463189682847907E-4	0.0	0.0	14.697031774325845	0.0
27	3.284252910463876E-4	0.0	0.0	15.058628019767918	0.0
28	4.1053161380798453E-4	0.0	0.0	15.436891848730594	0.0
29	4.1053161380798453E-4	0.0	0.0	15.813924082851848	0.0
30	4.1053161380798453E-4	0.0	0.0	16.236525326105788	0.0
31	4.926379365695814E-4	0.0	0.0	16.66290346020676	0.0
32	4.926379365695814E-4	0.0	0.0	17.073024542400937	0.0
33	4.926379365695814E-4	0.0	0.0	17.451863115622945	0.0
34	4.926379365695814E-4	0.0	0.0	17.819453122626616	0.0
35	4.926379365695814E-4	0.0	0.0	18.225304676037187	0.0
36	5.747442593311784E-4	0.0	0.0	18.639120542755634	0.0
37	5.747442593311784E-4	0.0	0.0	19.045793159393824	0.0
38	5.747442593311784E-4	0.0	0.0	19.427177028621443	0.0
39	5.747442593311784E-4	0.0	0.0	19.79517756723892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAGT	30	2.1656087E-6	45.000004	11
TAGGCCG	30	2.1656087E-6	45.000004	1
TACACGG	30	2.1656087E-6	45.000004	2
CGAACGT	20	7.033453E-4	45.0	37
ATTAGCG	70	0.0	45.0	1
CACGAAC	25	3.8909442E-5	45.0	43
TAACGAC	25	3.8909442E-5	45.0	10
TTATCCG	25	3.8909442E-5	45.0	1
CTGCGTA	20	7.033453E-4	45.0	24
GCCGATT	20	7.033453E-4	45.0	9
TGCGATA	25	3.8909442E-5	45.0	14
CATAGCG	20	7.033453E-4	45.0	1
CTACACG	20	7.033453E-4	45.0	27
CGGTCTA	20	7.033453E-4	45.0	31
CCGCAAT	20	7.033453E-4	45.0	33
GATAGTC	20	7.033453E-4	45.0	40
CGTTTTT	33005	0.0	44.366005	1
TCTAGCG	55	6.184564E-11	40.909092	1
TTCGACG	55	6.184564E-11	40.909092	25
GTACGAG	55	6.184564E-11	40.909092	1
>>END_MODULE